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The map label for this gene is fumB [H]

Identifier: 170681722

GI number: 170681722

Start: 4690644

End: 4692290

Strand: Reverse

Name: fumB [H]

Synonym: EcSMS35_4588

Alternate gene names: 170681722

Gene position: 4692290-4690644 (Counterclockwise)

Preceding gene: 170682748

Following gene: 170681045

Centisome position: 92.58

GC content: 54.4

Gene sequence:

>1647_bases
ATGTCAAACAAACCCTTTATCTACCAGGCACCTTTCCCGATGGGGAAAGACAATACCGAATACTATCTACTCACTTCCGA
TTACGTTAGCGTTGCCGACTTCGACGGTGAAACCATCCTGAAAGTGGAACCAGAAGCCCTGACTCTGCTGGCGCAGCAAG
CCTTTCACGATGCTTCTTTCATGCTCCGCCCGGCACACCAGAAACAGGTTGCGGCTATTCTTCACGATCCAGAAGCCAGC
GAAAACGACAAGTACGTGGCGCTGCAATTCTTAAGAAACTCCGAAATCGCCGCCAAAGGCGTGCTGCCGACCTGCCAGGA
TACCGGCACCGCGATCATCGTCGGTAAAAAAGGTCAGCGCGTGTGGACCGGTGGCGGTGATGAAGAAGCGCTGTCGAAAG
GCGTCTATAACACCTATATCGAAGATAACCTGCGCTATTCACAGAACGCGCCGCTGGACATGTATAAAGAGGTCAACACC
GGCACGAACCTGCCTGCGCAAATCGACCTGTACGCGGTAGATGGCGATGAGTACAAATTCCTCTGTGTCTCTAAAGGCGG
CGGCTCTGCCAACAAAACGTATCTCTACCAGGAAACCAAAGCCCTGCTGACGCCCGGCAAACTGAAAAACTTCCTCGTCG
AGAAAATGCGTACCCTCGGTACTGCAGCCTGCCCGCCGTACCATATCGCGTTTGTGATTGGCGGTACGTCTGCGGAAACC
AACCTGAAAACCGTCAAGTTAGCAAGCGCTCACTATTACGATGAACTGCCGACGGAAGGGAACGAACATGGTCAGGCGTT
CCGCGATATCCAGCTGGAACAGGAACTGCTGGAAGAGGCCCAGAAACTCGGTCTTGGCGCGCAGTTTGGCGGTAAATACT
TCGCGCACGACATCCGCGTTATCCGTCTGCCACGTCACGGCGCATCCTGCCCGGTAGGCATGGGCGTCTCCTGCTCCGCT
GACCGTAACATTAAAGCGAAAATCAACCGCGAAGGTATCTGGATCGAAAAACTGGAACACAATCCAGGCCAGTACATTCC
GGAAGAACTGCGCAAGGCCGGTGAAGGCGAAGCGGTGAAAGTTGACCTTAACCGCCCGATGAAAGAGATCCTCGCCCAGC
TTTCGCAATACCCGGTATCCACCCGCTTGTCGCTCACCGGCACCATTATCGTGGGTCGCGATATTGCACACGCCAAGCTG
AAAGAGCTGATTGACGCCGGTAAAGAACTGCCGCAGTACATCAAAGATCACCCGATCTACTACGCGGGTCCGGCGAAAAC
CCCTGCCGGTTATCCATCAGGTTCACTTGGCCCAACCACCGCAGGTCGTATGGACTCCTACGTGGATCTGCTGCAATCCC
ACGGCGGCAGCATGATCATGCTGGCGAAAGGTAACCGCAGTCAGCAGGTTACTGACGCGTGCCATAAACACGGCGGCTTC
TACCTCGGTAGCATCGGCGGTCCGGCGGCGGTACTGGCGCAGCAGAGCATCAAGCATTTGGAATGCGTCGCTTATCCGGA
GCTGGGTATGGAAGCTATCTGGAAAATCGAAGTAGAAGATTTCCCGGCGTTTATCCTGGTCGATGACAAAGGTAACGACT
TCTTCCAGCAAATCGTCAACAAACAGTGCGCGAACTGCACTAAGTAA

Upstream 100 bases:

>100_bases
CCGCGATGTACGGGTTCTTATAAATGCACTTTGCGTGCCGCCCGTGACTACGCGGCACGCCATTTTCGAATAACAAATAC
AGAGTTACAGGCTGGAAGCT

Downstream 100 bases:

>100_bases
CCTCTTCGGCCCAGCGCCTGGCAGCATGCTGCCAGGTGATCCCCCTGGGCCACCTCTTTTGCGATTGTAATTTCACGCTT
ACTGGTCAATAGTCAGTATT

Product: fumarate hydratase class I, anaerobic

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEPEALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEAS
ENDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
GTNLPAQIDLYAVDGDEYKFLCVSKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRTLGTAACPPYHIAFVIGGTSAET
NLKTVKLASAHYYDELPTEGNEHGQAFRDIQLEQELLEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSA
DRNIKAKINREGIWIEKLEHNPGQYIPEELRKAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVGRDIAHAKL
KELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGF
YLGSIGGPAAVLAQQSIKHLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK

Sequences:

>Translated_548_residues
MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEPEALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEAS
ENDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
GTNLPAQIDLYAVDGDEYKFLCVSKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRTLGTAACPPYHIAFVIGGTSAET
NLKTVKLASAHYYDELPTEGNEHGQAFRDIQLEQELLEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSA
DRNIKAKINREGIWIEKLEHNPGQYIPEELRKAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVGRDIAHAKL
KELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGF
YLGSIGGPAAVLAQQSIKHLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK
>Mature_547_residues
SNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEPEALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASE
NDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNTG
TNLPAQIDLYAVDGDEYKFLCVSKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRTLGTAACPPYHIAFVIGGTSAETN
LKTVKLASAHYYDELPTEGNEHGQAFRDIQLEQELLEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSAD
RNIKAKINREGIWIEKLEHNPGQYIPEELRKAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVGRDIAHAKLK
ELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGFY
LGSIGGPAAVLAQQSIKHLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=548, Percent_Identity=98.9051094890511, Blast_Score=1130, Evalue=0.0,
Organism=Escherichia coli, GI1787897, Length=548, Percent_Identity=90.3284671532847, Blast_Score=1034, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=181, Percent_Identity=29.8342541436464, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 60133; Mature: 60002

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEPEALTLLAQQAFHDASF
CCCCCEEEECCCCCCCCCCEEEEEECCCEEEEECCCCEEEEECHHHHHHHHHHHHCCCCE
MLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQR
EECCCHHHHHHHEEECCCCCCCCCEEEEEEECCCCCEECCCCCCCCCCCCEEEEECCCCE
VWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNTGTNLPAQIDLYAVDGDEYKF
EECCCCCHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEEECCCCEEE
LCVSKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRTLGTAACPPYHIAFVIGGTSAET
EEEECCCCCCCCEEEEECHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
NLKTVKLASAHYYDELPTEGNEHGQAFRDIQLEQELLEEAQKLGLGAQFGGKYFAHDIRV
CEEEEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECEEE
IRLPRHGASCPVGMGVSCSADRNIKAKINREGIWIEKLEHNPGQYIPEELRKAGEGEAVK
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHCCCCCEEE
VDLNRPMKEILAQLSQYPVSTRLSLTGTIIVGRDIAHAKLKELIDAGKELPQYIKDHPIY
EECCCCHHHHHHHHHHCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCEE
YAGPAKTPAGYPSGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGF
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQQSIKHLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIVN
EEECCCCHHHHHHHHHHHHHEEEECCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHH
KQCANCTK
HHHCCCCC
>Mature Secondary Structure 
SNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEPEALTLLAQQAFHDASF
CCCCEEEECCCCCCCCCCEEEEEECCCEEEEECCCCEEEEECHHHHHHHHHHHHCCCCE
MLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVLPTCQDTGTAIIVGKKGQR
EECCCHHHHHHHEEECCCCCCCCCEEEEEEECCCCCEECCCCCCCCCCCCEEEEECCCCE
VWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNTGTNLPAQIDLYAVDGDEYKF
EECCCCCHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEEECCCCEEE
LCVSKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRTLGTAACPPYHIAFVIGGTSAET
EEEECCCCCCCCEEEEECHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
NLKTVKLASAHYYDELPTEGNEHGQAFRDIQLEQELLEEAQKLGLGAQFGGKYFAHDIRV
CEEEEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECEEE
IRLPRHGASCPVGMGVSCSADRNIKAKINREGIWIEKLEHNPGQYIPEELRKAGEGEAVK
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHCCCCCEEE
VDLNRPMKEILAQLSQYPVSTRLSLTGTIIVGRDIAHAKLKELIDAGKELPQYIKDHPIY
EECCCCHHHHHHHHHHCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCEE
YAGPAKTPAGYPSGSLGPTTAGRMDSYVDLLQSHGGSMIMLAKGNRSQQVTDACHKHGGF
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQQSIKHLECVAYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIVN
EEECCCCHHHHHHHHHHHHHEEEECCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHH
KQCANCTK
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]