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The map label for this gene is epsJ [H]

Identifier: 170681557

GI number: 170681557

Start: 2283048

End: 2284208

Strand: Reverse

Name: epsJ [H]

Synonym: EcSMS35_2262

Alternate gene names: 170681557

Gene position: 2284208-2283048 (Counterclockwise)

Preceding gene: 170679591

Following gene: 170681490

Centisome position: 45.07

GC content: 44.96

Gene sequence:

>1161_bases
ATGTCTGAAAAAGAGAGTATAGCTGTTTCCGTTATTATCCCTGTGCACAATGCGGCGGGATATATTTCCGACACATTGAG
CACTGTTTTGTCGCAAACATTAAATGATATTGAAATCATTATTGTCAACGATTGTTCTGACGATAATACCTTAGAGATTG
TCTCGGCGCTGGCTGAAACTGATTCACGAATTAGAGTCATTAACAACACGACAAATCTTGGTGGGGGAGGCTCAAGAAAC
ATCGGGTTGGATGCTGCTACCGGTGAGTACGTTATTTTTCTTGATGACGACGATTATGTCGATAACATGATGTTGGAGCG
CATGTACGCACGTGCTAGCGATATACAAGCGGATGTCGTTATCTGTCGCAGCCAGTCTTTTGATCCCGCCTTGCAGGTTT
ATGCTCCGATGCCGTGGTCAGTGCGCCAGGAGCTGTTACCTGATCTCAAGTTTTTTTCATCCCAGGATATTCCCTCTGAC
TTTTTCCGCACCTTTGTCTGGTGGCCATGGGACAAGCTTCTCAGGCGTCAATTTATTACTAGTCATCAGCTTCGCTTTCA
GGAAATCAGGACGACCAACGATCTTTTCTTCGTCTGCGCATTTATGCTCATGGCGAACAGAATTTCGGTCTTAAATGAAA
CGTTAATTTCTCATACCATCAACCGTAGTGAATCCCTATCCGCCACGCGAGCAGAGTCGCACCGCTGTGCTGTTGAAGCA
TTGGTGGCGCTTAAAGCGTTTATCTGTCAGCAGGGGATGATGGAACACCGTCTCAGAGATTATAAAAACTATGTTGTCGT
GTTCCTTGAGTGGCATTTAAACACGATTTCTGGTCCGGCATTTCACCCGTTTTATCAGCAAGTGAAAGAGTTTGTTGTTG
CCCTGGATGCAAAGAGTGATGATTTTTATGATGAATTTATCGCCGCCGCCCATCATAGGATCACCACGCTGTCAGCTGAA
GAGTACCTATTCTCTCTTAAAGATCGGGTTCTGAAGGAACTTGAGTTTTTCCAGGCCAGGAGCTCCGCATTACAGCAAGA
AGTTGAGACGCTTACCCACTCATTAGCCGGGCAAAAGGATGAAAATGCGATATTGCATAATCAATTGCATGAGATTGAAG
AGCGGGTAACGGCTTTGTTGAATAAATCGAACTTTTGCTGA

Upstream 100 bases:

>100_bases
TTTCATCTTACCCATCCTTAATAAACTGGCAACCTACAAATTAAACGCTACCCATAAATAAATATAATTACGTTTATAAG
CTAAGCGTCAAGGTGATTGC

Downstream 100 bases:

>100_bases
GTTGAAGGATCAGATCACGTATCTTCCCGACAACGCAGACCGTTCCGTGGCAAAGCAAAAGTTCAAAATCACCAACTGGC
CCACCTACAATAAAGCCCTC

Product: glycosyl transferase, group 2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MSEKESIAVSVIIPVHNAAGYISDTLSTVLSQTLNDIEIIIVNDCSDDNTLEIVSALAETDSRIRVINNTTNLGGGGSRN
IGLDAATGEYVIFLDDDDYVDNMMLERMYARASDIQADVVICRSQSFDPALQVYAPMPWSVRQELLPDLKFFSSQDIPSD
FFRTFVWWPWDKLLRRQFITSHQLRFQEIRTTNDLFFVCAFMLMANRISVLNETLISHTINRSESLSATRAESHRCAVEA
LVALKAFICQQGMMEHRLRDYKNYVVVFLEWHLNTISGPAFHPFYQQVKEFVVALDAKSDDFYDEFIAAAHHRITTLSAE
EYLFSLKDRVLKELEFFQARSSALQQEVETLTHSLAGQKDENAILHNQLHEIEERVTALLNKSNFC

Sequences:

>Translated_386_residues
MSEKESIAVSVIIPVHNAAGYISDTLSTVLSQTLNDIEIIIVNDCSDDNTLEIVSALAETDSRIRVINNTTNLGGGGSRN
IGLDAATGEYVIFLDDDDYVDNMMLERMYARASDIQADVVICRSQSFDPALQVYAPMPWSVRQELLPDLKFFSSQDIPSD
FFRTFVWWPWDKLLRRQFITSHQLRFQEIRTTNDLFFVCAFMLMANRISVLNETLISHTINRSESLSATRAESHRCAVEA
LVALKAFICQQGMMEHRLRDYKNYVVVFLEWHLNTISGPAFHPFYQQVKEFVVALDAKSDDFYDEFIAAAHHRITTLSAE
EYLFSLKDRVLKELEFFQARSSALQQEVETLTHSLAGQKDENAILHNQLHEIEERVTALLNKSNFC
>Mature_385_residues
SEKESIAVSVIIPVHNAAGYISDTLSTVLSQTLNDIEIIIVNDCSDDNTLEIVSALAETDSRIRVINNTTNLGGGGSRNI
GLDAATGEYVIFLDDDDYVDNMMLERMYARASDIQADVVICRSQSFDPALQVYAPMPWSVRQELLPDLKFFSSQDIPSDF
FRTFVWWPWDKLLRRQFITSHQLRFQEIRTTNDLFFVCAFMLMANRISVLNETLISHTINRSESLSATRAESHRCAVEAL
VALKAFICQQGMMEHRLRDYKNYVVVFLEWHLNTISGPAFHPFYQQVKEFVVALDAKSDDFYDEFIAAAHHRITTLSAEE
YLFSLKDRVLKELEFFQARSSALQQEVETLTHSLAGQKDENAILHNQLHEIEERVTALLNKSNFC

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790044, Length=267, Percent_Identity=26.5917602996255, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 44099; Mature: 43968

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKESIAVSVIIPVHNAAGYISDTLSTVLSQTLNDIEIIIVNDCSDDNTLEIVSALAET
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHC
DSRIRVINNTTNLGGGGSRNIGLDAATGEYVIFLDDDDYVDNMMLERMYARASDIQADVV
CCCEEEEECCCCCCCCCCCCCCCEECCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEE
ICRSQSFDPALQVYAPMPWSVRQELLPDLKFFSSQDIPSDFFRTFVWWPWDKLLRRQFIT
EEECCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHH
SHQLRFQEIRTTNDLFFVCAFMLMANRISVLNETLISHTINRSESLSATRAESHRCAVEA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LVALKAFICQQGMMEHRLRDYKNYVVVFLEWHLNTISGPAFHPFYQQVKEFVVALDAKSD
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCC
DFYDEFIAAAHHRITTLSAEEYLFSLKDRVLKELEFFQARSSALQQEVETLTHSLAGQKD
HHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ENAILHNQLHEIEERVTALLNKSNFC
CCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SEKESIAVSVIIPVHNAAGYISDTLSTVLSQTLNDIEIIIVNDCSDDNTLEIVSALAET
CCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHC
DSRIRVINNTTNLGGGGSRNIGLDAATGEYVIFLDDDDYVDNMMLERMYARASDIQADVV
CCCEEEEECCCCCCCCCCCCCCCEECCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEE
ICRSQSFDPALQVYAPMPWSVRQELLPDLKFFSSQDIPSDFFRTFVWWPWDKLLRRQFIT
EEECCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHH
SHQLRFQEIRTTNDLFFVCAFMLMANRISVLNETLISHTINRSESLSATRAESHRCAVEA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LVALKAFICQQGMMEHRLRDYKNYVVVFLEWHLNTISGPAFHPFYQQVKEFVVALDAKSD
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCC
DFYDEFIAAAHHRITTLSAEEYLFSLKDRVLKELEFFQARSSALQQEVETLTHSLAGQKD
HHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ENAILHNQLHEIEERVTALLNKSNFC
CCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]