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The map label for this gene is pflB1 [H]

Identifier: 170681495

GI number: 170681495

Start: 3490352

End: 3492646

Strand: Reverse

Name: pflB1 [H]

Synonym: EcSMS35_3410

Alternate gene names: 170681495

Gene position: 3492646-3490352 (Counterclockwise)

Preceding gene: 170680069

Following gene: 170680786

Centisome position: 68.91

GC content: 52.9

Gene sequence:

>2295_bases
ATGAAGGTAGATATTGATACCAGCGATAAGCTGTACGCCGACGCATGGCTTGGCTTTAAAGGTACGGACTGGAAAAACGA
AATTAATGTCCGCGATTTTATTCAACATAACTATACACCGTATGAAGGTGATGAATCTTTTCTCGCCGAAGCAACGCCAG
CCACCACGGAATTGTGGGAAAAAGTGATGGAAGGCATCCGTATCGAAAACGCAACCCACGCGCCGGTTGATTTCGATACT
AATATTGCTACCACCATTACCGCTCATGATGCGGGATATATTAACCAGCCGCTGGAAAAAATAGTGGGCCTGCAAACGGA
TGCGCCATTAAAGCGTGCGCTGCATCCGTTCGGTGGCATTAATATGATTAAAAGTTCATTCCACGCCTATGGTCGTGAAA
TGGACAGTGAATTTGAATATCTGTTTACCGATCTGCGTAAAACCCATAACCAGGGCGTATTTGATGTTTACTCACCGGAT
ATGCTGCGCTGCCGTAAATCTGGCGTGCTGACCGGTTTACCAGATGGCTATGGTCGTGGGCGCATTATCGGTGACTATCG
CCGCGTGGCGCTATATGGCATCAGTTATCTGGTGCGTGAACGCGAACTGCAATTTGCCGATCTCCAGTCCCGTCTGGAAA
AAGGCGAGGATCTGGAAGCCACCATCCGTCTGCGTGAGGAGCTGGCGGAACATCGTCGTGCGCTGTTGCAGATTCAGGAA
ATGGCGGCGAAATACGGTTTTGATATCTCCCGTCCGGCGCAGAATGCGCAGGAAGCGGTGCAGTGGCTCTACTTCGCTTA
CCTGGCGGCAGTGAAATCACAGAACGGCGGCGCAATGTCTCTTGGCCGTACGGCATCGTTCCTCGATATCTACATTGAGC
GCGACTTTAAAGCTGGCGTACTCAATGAACAGCAGGCACAGGAACTGATCGACCACTTCATTATGAAGATCCGTATGGTG
CGCTTCCTGCGTACGCCGGAATTTGATTCGCTGTTCTCCGGCGACCCAATCTGGGCGACGGAAGTGATCGGCGGGATGGG
GCTGGACGGTCGTACGCTGGTGACCAAAAACTCTTTCCGTTATCTGCACACCCTGCACACTATGGGGCCAGCACCGGAAC
CAAACCTGACCATTCTGTGGTCGGAAGAATTACCGATCGCCTTCAAAAAATATGCCGCACAGGTGTCTATCGTCACCTCT
TCCTTGCAGTATGAAAACGACGATCTGATGCGTACTGACTTCAACAGCGACGATTACGCGATTGCCTGCTGCGTTAGCCC
GATGGTGATTGGTAAGCAAATGCAGTTCTTTGGTGCACGCGCTAACCTGGCGAAAACGCTGCTCTACGCAATTAACGGCG
GGGTGGATGAGAAGCTGAAGATTCAGGTCGGGCCGAAAACAGCACCATTGATGGACGATGTGCTGGACTACGACAAAGTG
ATGGACAGCCTCGATCATTTCATGGATTGGCTGGCAGTGCAGTACATCAGCGCGCTGAATATCATCCACTACATGCACGA
CAAGTACAGCTATGAAGCCTCGCTGATGGCGCTGCACGATCGTGATGTTTATCGCACGATGGCATGCGGCATCGCGGGCC
TGTCGGTGGCGACGGACTCCCTGTCTGCCATCAAATATGCCCGCGTGAAACCAATCCGTGACGAAAACGGCCTGGCGGTG
GACTTTGAAATCGACGGCGAATATCCGCAGTACGGTAACAACGACGAGCGCGTAGACAGCATTGCCTGCGACCTGGTTGA
ACGCTTTATGAAGAAAATTAAAGCGCTGCCAACCTATCGCAACGCCGTCCCTACCCAGTCGATTCTGACTATCACTTCTA
ACGTGGTGTACGGCCAGAAAACCGGTAACACGCCGGACGGTCGTCGCGCCGGAACACCGTTCGCACCGGGCGCTAACCCG
ATGCACGGTCGTGACCGCAAAGGTGCGGTAGCCTCGCTGACGTCGGTGGCGAAACTGCCGTTCACCTACGCCAAAGACGG
TATTTCGTACACCTTCTCAATTGTCCCGGCGGCGCTGGGCAAAGAAGATCCAGTACGTAAAACCAACCTTGTCGGCCTGC
TGGATGGGTATTTCCACCACGAAGCGGATGTCGAAGGCGGTCAACACCTCAACGTCAACGTAATGAATCGGGAAATGCTG
CTGGATGCCATCGAGCACCCGGAAAAATATCCTAACCTGACAATCCGTGTCTCTGGCTACGCCGTGCGCTTCAACGCACT
GACCCGTGAACAGCAGCAGGATGTTATTTCACGTACCTTTACCCAGGCGCTCTGA

Upstream 100 bases:

>100_bases
AGAAAAAATGATTGCTTTGGATGCCATTCATTTAGGCAAAGTGAACGCGCCCGCAGAATTTGCATAATTTAGTTGAAGTA
TTGTAGAGAGATTATTTTTC

Downstream 100 bases:

>100_bases
CGCCGGAGGATGTATGAAAAAGATTATCGAAACGCAACGTGCCCCAGGCGCAATCGGCCCTTATGTTCAAGGCGTTGATT
TAGGCAGCATGGTCTTCACC

Product: formate acetyltransferase

Products: CoA; pyruvate

Alternate protein names: Keto-acid formate-lyase [H]

Number of amino acids: Translated: 764; Mature: 764

Protein sequence:

>764_residues
MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGDESFLAEATPATTELWEKVMEGIRIENATHAPVDFDT
NIATTITAHDAGYINQPLEKIVGLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDLEATIRLREELAEHRRALLQIQE
MAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNGGAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMV
RFLRTPEFDSLFSGDPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYAAQVSIVTS
SLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLMDDVLDYDKV
MDSLDHFMDWLAVQYISALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAV
DFEIDGEYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANP
MHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKEDPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREML
LDAIEHPEKYPNLTIRVSGYAVRFNALTREQQQDVISRTFTQAL

Sequences:

>Translated_764_residues
MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGDESFLAEATPATTELWEKVMEGIRIENATHAPVDFDT
NIATTITAHDAGYINQPLEKIVGLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDLEATIRLREELAEHRRALLQIQE
MAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNGGAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMV
RFLRTPEFDSLFSGDPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYAAQVSIVTS
SLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLMDDVLDYDKV
MDSLDHFMDWLAVQYISALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAV
DFEIDGEYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANP
MHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKEDPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREML
LDAIEHPEKYPNLTIRVSGYAVRFNALTREQQQDVISRTFTQAL
>Mature_764_residues
MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGDESFLAEATPATTELWEKVMEGIRIENATHAPVDFDT
NIATTITAHDAGYINQPLEKIVGLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDLEATIRLREELAEHRRALLQIQE
MAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNGGAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMV
RFLRTPEFDSLFSGDPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYAAQVSIVTS
SLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLMDDVLDYDKV
MDSLDHFMDWLAVQYISALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAV
DFEIDGEYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANP
MHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKEDPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREML
LDAIEHPEKYPNLTIRVSGYAVRFNALTREQQQDVISRTFTQAL

Specific function: Anaerobic degradation of L-threonine to propionate. [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI48994926, Length=764, Percent_Identity=99.869109947644, Blast_Score=1588, Evalue=0.0,
Organism=Escherichia coli, GI1787131, Length=752, Percent_Identity=79.6542553191489, Blast_Score=1295, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=588, Percent_Identity=28.4013605442177, Blast_Score=182, Evalue=7e-47,
Organism=Escherichia coli, GI1790388, Length=641, Percent_Identity=25.8970358814353, Blast_Score=149, Evalue=9e-37,
Organism=Escherichia coli, GI1788933, Length=64, Percent_Identity=76.5625, Blast_Score=107, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: 2.3.1.54

Molecular weight: Translated: 85956; Mature: 85956

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGDESFLAEATPATTELWE
CEECCCCCCCEEEHEECCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHCCCCCHHHHHH
KVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIVGLQTDAPLKRALHPFGGI
HHHHCCEECCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCHHHHCCCCCCH
NMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPDMLRCRKSGVLTGLPDGYGRG
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHCCCCCCCCCCCCCC
RIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDLEATIRLREELAEHRRALLQIQE
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
MAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNGGAMSLGRTASFLDIYIERDFKAGV
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEECCCCCCC
LNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSGDPIWATEVIGGMGLDGRTLVTKNSFR
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHCCCCCCCCEEEECCCHH
YLHTLHTMGPAPEPNLTILWSEELPIAFKKYAAQVSIVTSSLQYENDDLMRTDFNSDDYA
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHEEEHHHCCCCCCCEECCCCCCCCE
IACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLMDDVLDYDKV
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHH
MDSLDHFMDWLAVQYISALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVATDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHH
LSAIKYARVKPIRDENGLAVDFEIDGEYPQYGNNDERVDSIACDLVERFMKKIKALPTYR
HHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
NAVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLP
CCCCCHHHHEEHHCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
FTYAKDGISYTFSIVPAALGKEDPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREML
CHHHCCCCEEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECHHHHH
LDAIEHPEKYPNLTIRVSGYAVRFNALTREQQQDVISRTFTQAL
HHHHHCHHHCCCEEEEEECEEEEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGDESFLAEATPATTELWE
CEECCCCCCCEEEHEECCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHCCCCCHHHHHH
KVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIVGLQTDAPLKRALHPFGGI
HHHHCCEECCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCHHHHCCCCCCH
NMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPDMLRCRKSGVLTGLPDGYGRG
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHCCCCCCCCCCCCCC
RIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDLEATIRLREELAEHRRALLQIQE
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
MAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNGGAMSLGRTASFLDIYIERDFKAGV
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEECCCCCCC
LNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSGDPIWATEVIGGMGLDGRTLVTKNSFR
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHCCCCCCCCEEEECCCHH
YLHTLHTMGPAPEPNLTILWSEELPIAFKKYAAQVSIVTSSLQYENDDLMRTDFNSDDYA
HHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHEEEHHHCCCCCCCEECCCCCCCCE
IACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLMDDVLDYDKV
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHH
MDSLDHFMDWLAVQYISALNIIHYMHDKYSYEASLMALHDRDVYRTMACGIAGLSVATDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHH
LSAIKYARVKPIRDENGLAVDFEIDGEYPQYGNNDERVDSIACDLVERFMKKIKALPTYR
HHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
NAVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLP
CCCCCHHHHEEHHCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
FTYAKDGISYTFSIVPAALGKEDPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREML
CHHHCCCCEEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECHHHHH
LDAIEHPEKYPNLTIRVSGYAVRFNALTREQQQDVISRTFTQAL
HHHHHCHHHCCCEEEEEECEEEEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA; formate

Specific reaction: acetyl-CoA + formate = CoA + pyruvate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 9484901 [H]