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The map label for this gene is cysW [H]

Identifier: 170681289

GI number: 170681289

Start: 2632273

End: 2633148

Strand: Reverse

Name: cysW [H]

Synonym: EcSMS35_2578

Alternate gene names: 170681289

Gene position: 2633148-2632273 (Counterclockwise)

Preceding gene: 170681836

Following gene: 170679900

Centisome position: 51.95

GC content: 56.74

Gene sequence:

>876_bases
ATGGCGGAAGTGACACAATTGAAGCGTTATGACGCGCGCCCGATTAACTGGGGCAAATGGTTTCTGATTGGCATCGGGAT
GCTGGTTTCGGCGTTCATCCTGCTGGTGCCGATGATTTACATCTTCGTGCAGGCGTTCAGCAAAGGGCTGATGCCGGTTT
TACAGAATCTGGCCGATCCGGACATGCTGCACGCCATCTGGCTGACGGTGATGATTGCGCTGATTGCCGTGCCGGTGAAC
CTGGTGTTCGGCATTCTGCTGGCCTGGCTGGTGACGCGCTTTAACTTCCCCGGACGCCAGTTGCTGCTGACGCTGCTGGA
CATTCCGTTTGCCGTGTCGCCGGTGGTTGCCGGTCTGGTGTATCTGCTGTTTTACGGCTCTAACGGCCCGCTCGGTGGCT
GGCTGGATGAACATAATCTGCAAATTATGTTCTCCTGGCCGGGAATGGTGCTGGTCACCATCTTCGTGACCTGTCCGTTT
GTGGTGCGCGAGCTGGTGCCGGTAATGTTAAGCCAGGGCAGCCAGGAAGACGAAACAGCGATTTTGCTGGGCGCATCTGG
CTGGCAGATGTTCCGTCGCGTCACGCTGCCGAACATCCGCTGGGCGCTGCTTTATGGCGTGGTGCTGACCAACGCCCGCG
CGATTGGCGAGTTTGGTGCGGTGTCGGTAGTTTCCGGCTCGATTCGCGGCGAAACACTTTCGCTGCCGTTACAGATTGAA
TTGCTGGAGCAGGACTACAACACCGTCGGCTCCTTTACCGCTGCCGCGTTGTTGACGCTGATGGCGATTATCACCCTGTT
TTTAAAGAGTATGTTGCAGTGGCGTCTGGAGAATCAGGAAAAACGCGCGCAGCAGGAGGAACATCATGAGCATTGA

Upstream 100 bases:

>100_bases
CGAGCGCGATTGCTTCGGTGATCCTCGCGGCATCCCTGTTGCTGCTGTTCTCAATTAACACTCTGCAAAGTCGCTTTGGT
CGGCGTGTGGTAGGTCATTA

Downstream 100 bases:

>100_bases
GATTGCCAATATTAAGAAGTCTTTTGGTCGCACCCAGGTGCTGAACGATATCTCACTGGATATTCCTTCTGGTCAGATGG
TCGCGTTGCTGGGGCCGTCC

Product: sulfate/thiosulfate transporter permease subunit

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQAFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVN
LVFGILLAWLVTRFNFPGRQLLLTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
VVRELVPVMLSQGSQEDETAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEFGAVSVVSGSIRGETLSLPLQIE
LLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRLENQEKRAQQEEHHEH

Sequences:

>Translated_291_residues
MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQAFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVN
LVFGILLAWLVTRFNFPGRQLLLTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
VVRELVPVMLSQGSQEDETAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEFGAVSVVSGSIRGETLSLPLQIE
LLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRLENQEKRAQQEEHHEH
>Mature_290_residues
AEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQAFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNL
VFGILLAWLVTRFNFPGRQLLLTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPFV
VRELVPVMLSQGSQEDETAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEFGAVSVVSGSIRGETLSLPLQIEL
LEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRLENQEKRAQQEEHHEH

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=291, Percent_Identity=99.6563573883162, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI1788764, Length=192, Percent_Identity=40.1041666666667, Blast_Score=147, Evalue=6e-37,
Organism=Escherichia coli, GI1786980, Length=232, Percent_Identity=34.051724137931, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32568; Mature: 32437

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQAFSKGLMPVLQNLADP
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
DMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLLLTLLDIPFAVSPVVAGLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHH
YLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPFVVRELVPVMLSQGSQEDETA
HHHHCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
ILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEFGAVSVVSGSIRGETLSLPLQIE
EEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCEEECCEEHH
LLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRLENQEKRAQQEEHHEH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC
>Mature Secondary Structure 
AEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQAFSKGLMPVLQNLADP
CCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
DMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLLLTLLDIPFAVSPVVAGLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHH
YLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPFVVRELVPVMLSQGSQEDETA
HHHHCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
ILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEFGAVSVVSGSIRGETLSLPLQIE
EEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCEEECCEEHH
LLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRLENQEKRAQQEEHHEH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]