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The map label for this gene is pepB [H]

Identifier: 170681265

GI number: 170681265

Start: 2750709

End: 2751992

Strand: Reverse

Name: pepB [H]

Synonym: EcSMS35_2675

Alternate gene names: 170681265

Gene position: 2751992-2750709 (Counterclockwise)

Preceding gene: 170682979

Following gene: 170682971

Centisome position: 54.3

GC content: 56.93

Gene sequence:

>1284_bases
ATGACAGAAGCGATGAAAATTACCCTCTCTACCCAACCTGCCGATGCGCGCTGGGGAGAAAAAGCAACTTACAGCATTAA
TAATGATGGCATTACCCTGCACTTGAACGGAGCGGACGATCTGGGGCTGATCCAGCGTGCGGCGCGCAAGATTGACGGTC
TGGGCATCAAACATGTTCAGCTAAGCGGTGAAGGCTGGGATGCGGATCGTTGCTGGGCATTCTGGCAAGGTTACAAAGCC
CCGAAAGGCACGCGTAAAGTGGAGTGGCCGGATCTGGACGATGTCCAGCGCCAGGAACTGGATAACCGCCTGATGATCAT
CGACTGGGTGCGTGACACCATTAACGCACCGGCGGAAGAGCTGGGGCCATCGCAACTGGCACAGCGTGCTGTTGATCTGA
TCAGCAATGTCGCGGGCGATCGTGTGACTTACCGGATCACCAAAGGCGAAGATCTGCGTGATCAAGGTTATATGGGGCTG
CACACGGTCGGACGCGGTTCAGAACGTTCTCCGGTATTGCTGGCGCTGGATTACAACCCGACTGGCGATAAAGAAGCGCC
AGTGTACGCGTGCCTGGTAGGTAAAGGTATCACTTTTGACTCCGGCGGCTACAGCATCAAACAGACCGCATTTATGGACT
CGATGAAGTCGGACATGGGCGGCGCGGCAACGGTTACCGGGGCGCTGGCGTTTGCCATTACGCGCGGACTGAACAAGCGC
GTGAAGCTGTTCCTCTGCTGTGCGGATAACCTGATCAGCGGCAACGCGTTCAAACTGGGCGATATTATTACCTATCGCAA
CGGTAAAAAAGTTGAAGTGATGAACACTGATGCGGAAGGGCGTCTGGTGCTGGCCGATGGCCTGATTGATGCCAGTGCGC
AGAAACCGGAACTGATCATTGATGCGGCGACCCTCACCGGGGCGGCGAAAACTGCGCTGGGTAATGATTATCACGCGCTG
TTCAGTTTTGACGATGCGCTTGCCGGTCGCTTGCTGGCGAGTGCCGCGCAGGAGAATGAACCGTTCTGGCGTCTGCCGCT
GGCGGAGTTCCACCGCAGCCAGCTGCCGTCTAACTTTGCCGAACTGAACAATACCGGAAGCGCGGCGTACCCGGCAGGCG
CGAGCACGGCGGCGGGCTTCCTGTCGCACTTTGTTGAGAACTATCAGCAAGGCTGGCTGCATATCGACTGCTCGGCGACT
TACCGTAAAGCGCCGGTTGAACAGTGGTCTGCGGGTGCTACGGGACTTGGTGTGCGCACGATTGCTAATCTGTTAACGGC
GTAA

Upstream 100 bases:

>100_bases
CTCGAAGCGATTTTGTTGGTCTGGCTGGACGAAGCCGAGTGAATGAACGGGCTGCCTGAGGGTAGCCCGTTTTGCTGTTA
AACGAATAAGGATAACTAAA

Downstream 100 bases:

>100_bases
ACTAATATGCCGGATGCGGCGTAAACGCCTTATCCGGACTACAAATTGCGAAAATCCAATAGATTGCGACTCCCTTGTAG
GCCTGATAAGCGTGGCGCAT

Product: aminopeptidase B

Products: NA

Alternate protein names: Aminopeptidase B [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLIQRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKA
PKGTRKVEWPDLDDVQRQELDNRLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGL
HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSDMGGAATVTGALAFAITRGLNKR
VKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNTDAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHAL
FSFDDALAGRLLASAAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGWLHIDCSAT
YRKAPVEQWSAGATGLGVRTIANLLTA

Sequences:

>Translated_427_residues
MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLIQRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKA
PKGTRKVEWPDLDDVQRQELDNRLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGL
HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSDMGGAATVTGALAFAITRGLNKR
VKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNTDAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHAL
FSFDDALAGRLLASAAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGWLHIDCSAT
YRKAPVEQWSAGATGLGVRTIANLLTA
>Mature_426_residues
TEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLIQRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAP
KGTRKVEWPDLDDVQRQELDNRLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGLH
TVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSDMGGAATVTGALAFAITRGLNKRV
KLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNTDAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALF
SFDDALAGRLLASAAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGWLHIDCSATY
RKAPVEQWSAGATGLGVRTIANLLTA

Specific function: Probably plays an important role in intracellular peptide degradation [H]

COG id: COG0260

COG function: function code E; Leucyl aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M17 family [H]

Homologues:

Organism=Homo sapiens, GI41393561, Length=317, Percent_Identity=36.2776025236593, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI47155554, Length=286, Percent_Identity=35.6643356643357, Blast_Score=137, Evalue=3e-32,
Organism=Escherichia coli, GI87082123, Length=427, Percent_Identity=99.0632318501171, Blast_Score=872, Evalue=0.0,
Organism=Escherichia coli, GI1790710, Length=315, Percent_Identity=38.0952380952381, Blast_Score=187, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17556903, Length=327, Percent_Identity=32.7217125382263, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17565172, Length=207, Percent_Identity=31.4009661835749, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI221379063, Length=344, Percent_Identity=31.9767441860465, Blast_Score=158, Evalue=8e-39,
Organism=Drosophila melanogaster, GI221379062, Length=344, Percent_Identity=31.9767441860465, Blast_Score=158, Evalue=8e-39,
Organism=Drosophila melanogaster, GI21357381, Length=344, Percent_Identity=31.9767441860465, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI24662227, Length=322, Percent_Identity=29.1925465838509, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI21355725, Length=289, Percent_Identity=29.757785467128, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24661038, Length=320, Percent_Identity=28.4375, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI161077148, Length=344, Percent_Identity=28.7790697674419, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI20130057, Length=344, Percent_Identity=28.7790697674419, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI20129969, Length=294, Percent_Identity=29.2517006802721, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21355645, Length=292, Percent_Identity=27.3972602739726, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24662223, Length=292, Percent_Identity=27.3972602739726, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19922386, Length=292, Percent_Identity=27.7397260273973, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI20129963, Length=288, Percent_Identity=28.125, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24646701, Length=228, Percent_Identity=29.8245614035088, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24646703, Length=228, Percent_Identity=29.8245614035088, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21358201, Length=228, Percent_Identity=29.8245614035088, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008330
- InterPro:   IPR011356
- InterPro:   IPR000819 [H]

Pfam domain/function: PF00883 Peptidase_M17 [H]

EC number: =3.4.11.23 [H]

Molecular weight: Translated: 46207; Mature: 46075

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00631 CYTOSOL_AP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLIQRAARKIDGLGIKHVQ
CCCEEEEEEECCCCCCCCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEE
LSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDVQRQELDNRLMIIDWVRDTINAPAEE
ECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEHHHHHCCCHHH
LGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGLHTVGRGSERSPVLLALDYNP
CCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCEEEEEECCC
TGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSDMGGAATVTGALAFAITRGLNKR
CCCCCCCEEEEEECCCEEECCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCE
VKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNTDAEGRLVLADGLIDASAQKPELII
EEEEEEECCCCCCCCCEEECCEEEECCCCEEEEEECCCCCEEEEECCCCCCCCCCCEEEE
DAATLTGAAKTALGNDYHALFSFDDALAGRLLASAAQENEPFWRLPLAEFHRSQLPSNFA
EEHHHCCHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEECCHHHHHHHHCCHHHH
ELNNTGSAAYPAGASTAAGFLSHFVENYQQGWLHIDCSATYRKAPVEQWSAGATGLGVRT
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHCCCCCCCHHHH
IANLLTA
HHHHHCC
>Mature Secondary Structure 
TEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLIQRAARKIDGLGIKHVQ
CCEEEEEEECCCCCCCCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEE
LSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDVQRQELDNRLMIIDWVRDTINAPAEE
ECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEHHHHHCCCHHH
LGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGLHTVGRGSERSPVLLALDYNP
CCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCEEEEEECCC
TGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSDMGGAATVTGALAFAITRGLNKR
CCCCCCCEEEEEECCCEEECCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCE
VKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNTDAEGRLVLADGLIDASAQKPELII
EEEEEEECCCCCCCCCEEECCEEEECCCCEEEEEECCCCCEEEEECCCCCCCCCCCEEEE
DAATLTGAAKTALGNDYHALFSFDDALAGRLLASAAQENEPFWRLPLAEFHRSQLPSNFA
EEHHHCCHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEECCHHHHHHHHCCHHHH
ELNNTGSAAYPAGASTAAGFLSHFVENYQQGWLHIDCSATYRKAPVEQWSAGATGLGVRT
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHCCCCCCCHHHH
IANLLTA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]