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The map label for this gene is yafC [H]

Identifier: 170681248

GI number: 170681248

Start: 239093

End: 240007

Strand: Reverse

Name: yafC [H]

Synonym: EcSMS35_0221

Alternate gene names: 170681248

Gene position: 240007-239093 (Counterclockwise)

Preceding gene: 170681258

Following gene: 170681875

Centisome position: 4.74

GC content: 49.29

Gene sequence:

>915_bases
ATGAAAGCCACGTCGGAAGAACTCGCCATTTTTGTTTCAGTCGTCGAAAGCGGCAGCTTTAGCCGGGCAGCGGAACAATT
AGGGCAAGCAAACTCAGCGGTAAGCCGGGCGGTGAAAAAGCTGGAGATGAAACTTGGCGTTAGCCTGCTTAATCGGACCA
CGCGGCAACTTAGCCTGACGGAAGAAGGCGAGCGTTATTTTCGTCGCGTACAGTCAATTTTGCAGGAGATGGCAGCGGCA
GAATCAGAAATTATGGAGACGCGTAATACACCGCGTGGACTGTTACGGATCGATGCCGCAACTCCAGTGGTGCTGCACTT
TCTGATGCCGTTGATTAAGCCTTTTCGTGAACGCTATCCGGAAGTCACTTTATCGCTAGTCTCCTCCGAAACGATTATTA
ATTTGATCGAAAGAAAAGTGGATGTCGCGATACGCGCCGGTACGTTAACGGATTCCAGCTTACGTGCCAGGCCGTTATTT
AACAGTTATCGAAAAATTATCGCCTCCCCCGATTATATTTCCCGCTACGGGAAGCCAGAAACGATCGACGATTTAAAGCA
ACATATTTGCCTGGGATTCACTGAACCCGCTTCCCTCAATACCTGGCCGATAGCCTGTAGCGATGGACAATTACATGAGG
TGAAGTACGGTTTGTCATCCAATAGTGGGGAAACACTGAAACAGCTTTGCCTGAGTGGCAACGGGATTGCGTGTTTGTCC
GACTACATGATCGACAGAGAAATCGCTCGCGGAGAATTGGTGGAGTTAATGGCAGATAAAGTATTGCCAGTGGAAATGCC
ATTCAGTGCCGTATATTACAGCGACCGTGCGGTAAGTACACGCATCCGGGCTTTTATCGATTTCCTTAGCGAGCATGTAA
AAACAGCTCCCGGAGGAGCTGTCAGAGAGGCTTAA

Upstream 100 bases:

>100_bases
CATTATTGAGAACATTAAATCGGATGACATATCTCGCATCCTGATGGTTTAATGACAATTAAATTCAAAATATCTCTTCT
ATTTTGCAACAGGAGCAAAA

Downstream 100 bases:

>100_bases
TCCCATTCAGGAGCCAGACCTTCCGGGCTAACCAGGCGGTCGTTGCAATCCAGTGCGGCGATCGCTTTTTTATCTTCGGC
ATCAAGCTGTAAATTTTGTG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAA
ESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIACSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLS
DYMIDREIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPGGAVREA

Sequences:

>Translated_304_residues
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAA
ESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIACSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLS
DYMIDREIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPGGAVREA
>Mature_304_residues
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAA
ESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIACSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLS
DYMIDREIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPGGAVREA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=304, Percent_Identity=99.3421052631579, Blast_Score=616, Evalue=1e-178,
Organism=Escherichia coli, GI87081978, Length=289, Percent_Identity=33.5640138408304, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787128, Length=296, Percent_Identity=28.7162162162162, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=29.3515358361775, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1789639, Length=289, Percent_Identity=31.4878892733564, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1789440, Length=290, Percent_Identity=28.6206896551724, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1787589, Length=288, Percent_Identity=28.8194444444444, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1789173, Length=284, Percent_Identity=28.169014084507, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1788706, Length=298, Percent_Identity=25.8389261744966, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI157672245, Length=171, Percent_Identity=32.7485380116959, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788481, Length=292, Percent_Identity=24.3150684931507, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87082132, Length=304, Percent_Identity=27.6315789473684, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786448, Length=254, Percent_Identity=27.1653543307087, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI145693105, Length=165, Percent_Identity=29.0909090909091, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1788748, Length=126, Percent_Identity=34.1269841269841, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786508, Length=81, Percent_Identity=40.7407407407407, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787806, Length=169, Percent_Identity=25.4437869822485, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1789506, Length=174, Percent_Identity=27.5862068965517, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33751; Mature: 33751

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLT
CCCCHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
EEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
EVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLFNSYRKIIASPDYISRYGKPE
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCC
TIDDLKQHICLGFTEPASLNTWPIACSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLS
CHHHHHHHHHCCCCCCCCCCCCCEEECCCCEEHEEECCCCCCCHHHHHHHHCCCCHHHHH
DYMIDREIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPGGA
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEECCHHHHHHHHHHHHHHHHHHHCCCCCC
VREA
CCCC
>Mature Secondary Structure
MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMKLGVSLLNRTTRQLSLT
CCCCHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
EEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATPVVLHFLMPLIKPFRERYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
EVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLFNSYRKIIASPDYISRYGKPE
HHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCC
TIDDLKQHICLGFTEPASLNTWPIACSDGQLHEVKYGLSSNSGETLKQLCLSGNGIACLS
CHHHHHHHHHCCCCCCCCCCCCCEEECCCCEEHEEECCCCCCCHHHHHHHHCCCCHHHHH
DYMIDREIARGELVELMADKVLPVEMPFSAVYYSDRAVSTRIRAFIDFLSEHVKTAPGGA
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEECCHHHHHHHHHHHHHHHHHHHCCCCCC
VREA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]