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The map label for this gene is lpfB [H]

Identifier: 170681205

GI number: 170681205

Start: 4189983

End: 4190714

Strand: Reverse

Name: lpfB [H]

Synonym: EcSMS35_4095

Alternate gene names: 170681205

Gene position: 4190714-4189983 (Counterclockwise)

Preceding gene: 170680528

Following gene: 170684256

Centisome position: 82.68

GC content: 43.85

Gene sequence:

>732_bases
ATGTCCAGAAAAAGTTGTTTAATCAGGCTCTTTAAAACAACCGTTTCATGTAAGTTATTTACCGCGATTATACTCAGTGT
TTTTTTAGTCCAACCAGCATTAACCTATGCAGGCGTTGTCATTGGTGGTACACGGGTAGTTTACTTAAGTAATAACCCAG
ATAAATCTATTTCTGTATTTAGCAAGGAAGAAAAAATTCCTTATCTCATCCAGGCATGGGTAGATCCGTTTAATAAAGAG
GATAAAAGCAAAGCACCGTTTACCGTTATTCCTCCGGTATCACGATTAGAACCCGCGCAAGAAAAAGTATTACGCATTAT
TCATACCAAAGGTGTGTCATTGCCTGATGATCGCGAGTCAGTCTTCTGGCTGAATATTAAAAATATTCCGCCCTCTGCGA
GCAACAAAGCGGCGAACTCACTGGAAATCGCAGTTAAAACGCGTATCAAGCTGTTCTGGCGACCGGCAAGCATTCGCCTG
ATTCCCGAAGATGCTGCACCGAAGGTGAAGTGGCACCGGGAAGGGCGAAACCTGATTGCAGAGAATCCCAACCCGATTCA
TATCAGTGTGATGGATGTCATCGTTGATGGTCATGATGTGCCCCTCAACATGCTCCGTCCTTTTGAAACGCTCACGTTAC
CTTTACCTGCTAATTCTGCGGGAGGCCAAATGATCTGGCGATTTATTAATGATTACGGCGCAGTCAGTGACCCGATAAAA
ATGACGCTGTAA

Upstream 100 bases:

>100_bases
CGGCGGGTACGGCGAACGCCGATTCACAATTTACTGTCGAATATAAGAAATAATTTATCAAGGGCCTTTTTATTCAGTTG
GCTTTGGCCAGGGAGTCGTT

Downstream 100 bases:

>100_bases
GCAAGGGAAAAGGATAGGCGTATTATGATGACGACCAGAATAAAGGTTGGCCTCACGGCAGGGACGTGTCTGATTTTCTC
GCAAAGCCTGATGGCCGAGG

Product: long polar fimbrial operon protein LpfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MSRKSCLIRLFKTTVSCKLFTAIILSVFLVQPALTYAGVVIGGTRVVYLSNNPDKSISVFSKEEKIPYLIQAWVDPFNKE
DKSKAPFTVIPPVSRLEPAQEKVLRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKAANSLEIAVKTRIKLFWRPASIRL
IPEDAAPKVKWHREGRNLIAENPNPIHISVMDVIVDGHDVPLNMLRPFETLTLPLPANSAGGQMIWRFINDYGAVSDPIK
MTL

Sequences:

>Translated_243_residues
MSRKSCLIRLFKTTVSCKLFTAIILSVFLVQPALTYAGVVIGGTRVVYLSNNPDKSISVFSKEEKIPYLIQAWVDPFNKE
DKSKAPFTVIPPVSRLEPAQEKVLRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKAANSLEIAVKTRIKLFWRPASIRL
IPEDAAPKVKWHREGRNLIAENPNPIHISVMDVIVDGHDVPLNMLRPFETLTLPLPANSAGGQMIWRFINDYGAVSDPIK
MTL
>Mature_242_residues
SRKSCLIRLFKTTVSCKLFTAIILSVFLVQPALTYAGVVIGGTRVVYLSNNPDKSISVFSKEEKIPYLIQAWVDPFNKED
KSKAPFTVIPPVSRLEPAQEKVLRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKAANSLEIAVKTRIKLFWRPASIRLI
PEDAAPKVKWHREGRNLIAENPNPIHISVMDVIVDGHDVPLNMLRPFETLTLPLPANSAGGQMIWRFINDYGAVSDPIKM
TL

Specific function: Involved in the biogenesis of the F1C fimbriae [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1790771, Length=237, Percent_Identity=33.3333333333333, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1789532, Length=228, Percent_Identity=36.4035087719298, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1787171, Length=231, Percent_Identity=33.3333333333333, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1786743, Length=222, Percent_Identity=29.7297297297297, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1786333, Length=240, Percent_Identity=34.5833333333333, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1786936, Length=202, Percent_Identity=30.6930693069307, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1788677, Length=199, Percent_Identity=33.6683417085427, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788428, Length=234, Percent_Identity=31.1965811965812, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87082203, Length=215, Percent_Identity=25.1162790697674, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081806, Length=207, Percent_Identity=29.4685990338164, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 27206; Mature: 27075

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKSCLIRLFKTTVSCKLFTAIILSVFLVQPALTYAGVVIGGTRVVYLSNNPDKSISVF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCEEEEEEECCCCCCEEEE
SKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPAQEKVLRIIHTKGVSLPDDRES
ECCCCCCHHHHHHCCCCCCCCCCCCCEEEECCHHHCCHHHHHHHHHHHHCCCCCCCCCCC
VFWLNIKNIPPSASNKAANSLEIAVKTRIKLFWRPASIRLIPEDAAPKVKWHREGRNLIA
EEEEEECCCCCCCCCCCCCEEEEEEEEEEEEEEECCEEEEECCCCCCCCEEECCCCCEEC
ENPNPIHISVMDVIVDGHDVPLNMLRPFETLTLPLPANSAGGQMIWRFINDYGAVSDPIK
CCCCEEEEEEEEEEECCCCCCHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCEE
MTL
ECC
>Mature Secondary Structure 
SRKSCLIRLFKTTVSCKLFTAIILSVFLVQPALTYAGVVIGGTRVVYLSNNPDKSISVF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCEEEEEEECCCCCCEEEE
SKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPAQEKVLRIIHTKGVSLPDDRES
ECCCCCCHHHHHHCCCCCCCCCCCCCEEEECCHHHCCHHHHHHHHHHHHCCCCCCCCCCC
VFWLNIKNIPPSASNKAANSLEIAVKTRIKLFWRPASIRLIPEDAAPKVKWHREGRNLIA
EEEEEECCCCCCCCCCCCCEEEEEEEEEEEEEEECCEEEEECCCCCCCCEEECCCCCEEC
ENPNPIHISVMDVIVDGHDVPLNMLRPFETLTLPLPANSAGGQMIWRFINDYGAVSDPIK
CCCCEEEEEEEEEEECCCCCCHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCEE
MTL
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]