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The map label for this gene is cusS [H]

Identifier: 170681031

GI number: 170681031

Start: 595937

End: 597379

Strand: Reverse

Name: cusS [H]

Synonym: EcSMS35_0587

Alternate gene names: 170681031

Gene position: 597379-595937 (Counterclockwise)

Preceding gene: 170681461

Following gene: 170680338

Centisome position: 11.79

GC content: 51.28

Gene sequence:

>1443_bases
ATGGTCAGTAAGCCATTTCAGCGCCCGTTTTCGCTGGCAACCCGTCTGACCTTTTTTATCAGCCTGGCCACCATCGCGGC
GTTTTTCGCCTTTGCATGGATCATGATCCACTCGGTAAAAGTGCATTTTGCCGAGCAGGATATTAATGATTTAAAAGAGA
TTAGCGCCACCCTTGAACGGGTACTTAATCACCCTGACGAAACGCAAGCCCGACGCTTAATGACGCTGGAAGATATCGTC
AGTGGTTATTCCAACGTGTTGATTTCTCTGGTAGATAGCCACGGTAAAACGGTGTATCACTCCCCCGGTGCGCCGGATAT
CCGCGAGTTTGCACGTGATGCCATACCCGATAAAGACGCTCGGGGCGGCGAGGTGTATCTCCTTTCCGGCCCGACAATGA
TGATGCCAGGCCACGGTCACGGACATATGGAACACAGCAACTGGCGGATGATTAACTTGCCGGTTGGCCCGCTGGTGGAC
GGCAAACCGATTTATACGCTCTACATCGCGCTGTCTATCGATTTTCATCTTCATTACATTAATGATTTGATGAATAAACT
TATTATGACCGCATCGGTAATCAGCATTCTGATCGTCTTTATCGTGCTGTTGGCGGTACATAAAGGTCACGCGCCGATCC
GCAGCGTCAGCCGTCAAATCCAGAACATTACCTCGAAAGATCTCGACGTTCGCCTCGATCCACAGACCGTGCCCATTGAA
CTCGAACAACTGGTGCTGTCGTTCAACCATATGATCGAGCGTATTGAGGATGTCTTTACCCGCCAGTCCAATTTCTCAGC
GGATATCGCCCACGAAATTCGTACACCAATTACGAATCTCATAACGCAAACGGAAATCGCCCTCAGCCAGTCGCGCAGCC
AGAAGGAGCTGGAAGATGTGCTCTACTCTAATCTCGAAGAGCTGACGCGAATGGCGAAAATGGTCAGCGATATGTTGTTT
CTCGCCCAGGCCGATAACAACCAGTTAATCCCCGAAAAGAAAATGCTCAACCTGGCGGATGAAGTCGGCAAAGTGTTCGA
TTTTTTCGAGGCGTTAGCGGAAGATCGCGGCGTGGAGTTGCGGTTTGTTGGTGACGAGTGTCAGGTTGCGGGCGATCCGC
TGATGTTGCGTCGGGCGTTAAGCAACCTACTTTCTAATGCCCTGCGTTATACGCCAACCGGAGAGACAATTGTCGTGCGC
TGCCAGACGGTCGATCACCTGGTGCAAGTTACCGTCGAAAACCCTGGTACGCCCATTGCGCCTGAGCACTTACCGCGATT
GTTTGACCGTTTCTACCGCATTGATCCTTCCCGCCAGCGAAAAGGCGAAGGTAGCGGTATTGGCCTGGCGATAGTGAAAT
CGATTGTTGTCGCGCATAAAGGCACGGTTGCGGTAACGTCAGATGCGCGGGGAACAAGGTTTGTTATCATATTACCCGCT
TAA

Upstream 100 bases:

>100_bases
GATGTGGCGGTGAAGCGGCTACGCGGCAAAATCGACAACGACTTTGAGCCGAAGCTGATTCAGACCGTGCGCGGCGTGGG
CTATATGCTTGAGGTGCCGG

Downstream 100 bases:

>100_bases
TCCTTCAGCAAACGGCAACTTTTATAACCAGTGTAAAAATAACGTGCCGCAATAATCCTACCTATTAATGATAAAAAGCT
GTCACAATTCATAAAAAACT

Product: sensor kinase CusS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLERVLNHPDETQARRLMTLEDIV
SGYSNVLISLVDSHGKTVYHSPGAPDIREFARDAIPDKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLF
LAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTPTGETIVVR
CQTVDHLVQVTVENPGTPIAPEHLPRLFDRFYRIDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVIILPA

Sequences:

>Translated_480_residues
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLERVLNHPDETQARRLMTLEDIV
SGYSNVLISLVDSHGKTVYHSPGAPDIREFARDAIPDKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLF
LAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTPTGETIVVR
CQTVDHLVQVTVENPGTPIAPEHLPRLFDRFYRIDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVIILPA
>Mature_480_residues
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLERVLNHPDETQARRLMTLEDIV
SGYSNVLISLVDSHGKTVYHSPGAPDIREFARDAIPDKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLF
LAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTPTGETIVVR
CQTVDHLVQVTVENPGTPIAPEHLPRLFDRFYRIDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVIILPA

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=480, Percent_Identity=98.3333333333333, Blast_Score=972, Evalue=0.0,
Organism=Escherichia coli, GI1788279, Length=296, Percent_Identity=33.445945945946, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1786600, Length=263, Percent_Identity=33.0798479087452, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1788393, Length=271, Percent_Identity=30.6273062730627, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1790346, Length=332, Percent_Identity=30.1204819277108, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1787894, Length=311, Percent_Identity=29.5819935691318, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1789403, Length=282, Percent_Identity=31.2056737588652, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI145693157, Length=237, Percent_Identity=27.0042194092827, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1790551, Length=298, Percent_Identity=28.1879194630872, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789808, Length=283, Percent_Identity=27.5618374558304, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789149, Length=236, Percent_Identity=27.5423728813559, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790436, Length=220, Percent_Identity=25.9090909090909, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081816, Length=251, Percent_Identity=26.6932270916335, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788713, Length=230, Percent_Identity=28.695652173913, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1787374, Length=347, Percent_Identity=23.6311239193084, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790861, Length=298, Percent_Identity=25.503355704698, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI48994928, Length=240, Percent_Identity=25.4166666666667, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87082128, Length=296, Percent_Identity=24.3243243243243, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786912, Length=242, Percent_Identity=25.6198347107438, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53789; Mature: 53789

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLER
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHH
VLNHPDETQARRLMTLEDIVSGYSNVLISLVDSHGKTVYHSPGAPDIREFARDAIPDKDA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCC
RGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVDGKPIYTLYIALSIDFHLHYI
CCCEEEEEECCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEEHHHHHHH
NDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHH
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVEL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
RFVGDECQVAGDPLMLRRALSNLLSNALRYTPTGETIVVRCQTVDHLVQVTVENPGTPIA
EEECCHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCCCC
PEHLPRLFDRFYRIDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVIILPA
HHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECC
>Mature Secondary Structure
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLER
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHH
VLNHPDETQARRLMTLEDIVSGYSNVLISLVDSHGKTVYHSPGAPDIREFARDAIPDKDA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCC
RGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVDGKPIYTLYIALSIDFHLHYI
CCCEEEEEECCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEEHHHHHHH
NDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHH
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDV
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVEL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
RFVGDECQVAGDPLMLRRALSNLLSNALRYTPTGETIVVRCQTVDHLVQVTVENPGTPIA
EEECCHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCCCC
PEHLPRLFDRFYRIDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVIILPA
HHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]