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The map label for this gene is gyrB [H]

Identifier: 170680955

GI number: 170680955

Start: 4154373

End: 4156787

Strand: Reverse

Name: gyrB [H]

Synonym: EcSMS35_4064

Alternate gene names: 170680955

Gene position: 4156787-4154373 (Counterclockwise)

Preceding gene: 170684195

Following gene: 170682981

Centisome position: 82.01

GC content: 54.87

Gene sequence:

>2415_bases
ATGTCGAATTCTTATGACTCCTCCAGTATCAAAGTCCTGAAAGGGCTGGATGCGGTGCGTAAGCGCCCGGGTATGTATAT
CGGCGACACGGATGACGGCACCGGTCTGCACCACATGGTATTCGAGGTGGTAGATAACGCTATCGACGAAGCGCTCGCGG
GTCACTGTAAAGAAATTATCGTCACCATTCACGCCGATAACTCTGTCTCTGTACAGGATGACGGGCGCGGCATTCCGACC
GGTATTCACCCGGAAGAGGGCGTATCGGCGGCGGAAGTGATCATGACCGTTCTGCACGCAGGCGGTAAATTTGACGATAA
CTCCTATAAAGTGTCCGGCGGTCTGCACGGCGTTGGTGTTTCGGTAGTAAACGCCCTGTCGCAAAAACTGGAGCTGGTTA
TCCAGCGCGAGGGTAAAATTCACCGTCAGATCTACGAACACGGTGTACCGCAGGCCCCGCTGGCGGTTACCGGCGAGACT
GAAAAAACCGGCACCATGGTGCGTTTCTGGCCAAGCCTTGAAACCTTCACCAATGTGACCGAGTTCGAATATGACATTCT
GGCGAAACGTCTGCGTGAGTTGTCGTTCCTCAACTCCGGCGTTTCCATTCGTCTGCGCGATAAGCGCGACGGCAAAGAAG
ACCACTTCCACTATGAAGGCGGCATCAAGGCGTTCGTTGAATATCTGAACAAGAACAAAACGCCGATCCACCCGAATATC
TTCTACTTCTCCACTGAAAAAGACGGTATTGGCGTCGAAGTGGCGTTGCAGTGGAACGATGGCTTCCAGGAAAACATCTA
CTGCTTTACCAACAACATTCCGCAGCGTGACGGCGGTACTCACCTGGCAGGCTTCCGTGCGGCGATGACCCGTACCCTGA
ACGCCTACATGGACAAAGAAGGCTACAGCAAAAAAGCCAAAGTCAGCGCCACCGGTGACGATGCGCGTGAAGGCCTGATT
GCGGTCGTTTCCGTGAAAGTGCCGGACCCGAAATTCTCCTCCCAGACCAAAGACAAACTGGTTTCTTCTGAGGTGAAATC
GGCGGTTGAACAGCAGATGAACGAACTGCTGGCGGAATACCTGCTGGAAAACCCAACCGACGCGAAAATCGTGGTTGGCA
AAATTATCGATGCTGCCCGTGCCCGTGAAGCTGCGCGTCGCGCGCGTGAAATGACCCGCCGTAAAGGTGCGCTCGACTTA
GCGGGCCTGCCGGGCAAACTGGCAGACTGCCAGGAACGCGATCCGGCGCTCTCTGAACTGTACCTGGTGGAAGGGGACTC
CGCGGGCGGCTCTGCGAAGCAGGGGCGTAACCGCAAGAACCAGGCGATTCTGCCGCTGAAGGGTAAAATCCTCAACGTTG
AGAAAGCGCGCTTCGATAAGATGCTCTCTTCTCAGGAAGTGGCGACGCTGATCACCGCGCTTGGCTGTGGTATCGGTCGT
GACGAGTACAACCCGGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCGGACGTCGACGGCTCGCACATTCG
TACGCTGCTGTTGACCTTCTTCTATCGTCAGATGCCGGAAATCGTTGAACGCGGTCACGTCTACATCGCTCAGCCGCCGC
TGTACAAAGTGAAGAAAGGCAAGCAGGAACAGTACATTAAAGACGACGAAGCGATGGATCAGTACCAGATCTCTATCGCG
CTGGATGGCGCAACGCTGCACACCAACGCCAGTGCACCGGCGCTGGCTGGCGAAGCGTTAGAGAAACTGGTGTCTGAGTA
CAACGCGACGCAGAAAATGATCAACCGCATGGAGCGTCGTTATCCGAAAGCAATGCTGAAAGAGCTTATCTATCAGCCGA
CGTTGACGGAAACCGACCTTTCTGACGAGCAAACCGTTACCCGCTGGGTGAACGCGCTGGTCAGCGAACTGAACGATAAA
GAACAGCACGGCAGCCAGTGGAAGTTTGATGTCCATACCAATGCCGAACAAAACCTGTTCGAGCCGATTGTTCGCGTGCG
TACCCACGGTGTGGATACTGACTATCCGCTGGATCACGAATTTATCACCGGCGGCGAATATCGTCGTATCTGCACGCTGG
GTGAGAAACTGCGTGGCTTGCTGGAAGAAGATGCATTTATCGAACGTGGCGAACGTCGTCAGCCGGTAGCCAGCTTCGAG
CAGGCGCTGGACTGGCTGGTGAAAGAGTCCCGTCGCGGCCTCTCCATCCAGCGTTATAAAGGTCTGGGCGAGATGAACCC
GGAACAGCTGTGGGAAACCACCATGGATCCGGAAAGCCGTCGTATGCTGCGCGTTACCGTTAAAGATGCGATTGCTGCCG
ACCAGTTGTTCACTACGCTTATGGGCGACGCCGTTGAACCGCGCCGTGCGTTTATCGAAGAGAACGCCCTGAAAGCGGCA
AATATCGATATTTAA

Upstream 100 bases:

>100_bases
GAACACGTTATAGACATGTCGGACGAAAATTCGAAGATGTTTACCGTGGAAAAGGGTAAAATAACGGATTAACCCAAGTA
TAAATGAGCGAGAAACGTTG

Downstream 100 bases:

>100_bases
TGGCTTTTTGTTGCTGATAAAATGACTTAGTCGTCAGTCCGGGCGGAGATCATCTCCGCCCTTTTTATTTCTGCAATCCG
CAATTATCATCCCGCAGATC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 804; Mature: 803

Protein sequence:

>804_residues
MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPT
GIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
EKTGTMVRFWPSLETFTNVTEFEYDILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHYEGGIKAFVEYLNKNKTPIHPNI
FYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQRDGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLI
AVVSVKVPDPKFSSQTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMTRRKGALDL
AGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGR
DEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIA
LDGATLHTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTETDLSDEQTVTRWVNALVSELNDK
EQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFITGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFE
QALDWLVKESRRGLSIQRYKGLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALKAA
NIDI

Sequences:

>Translated_804_residues
MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPT
GIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
EKTGTMVRFWPSLETFTNVTEFEYDILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHYEGGIKAFVEYLNKNKTPIHPNI
FYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQRDGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLI
AVVSVKVPDPKFSSQTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMTRRKGALDL
AGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGR
DEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIA
LDGATLHTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTETDLSDEQTVTRWVNALVSELNDK
EQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFITGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFE
QALDWLVKESRRGLSIQRYKGLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALKAA
NIDI
>Mature_803_residues
SNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTG
IHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGETE
KTGTMVRFWPSLETFTNVTEFEYDILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHYEGGIKAFVEYLNKNKTPIHPNIF
YFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQRDGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIA
VVSVKVPDPKFSSQTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMTRRKGALDLA
GLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGRD
EYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIAL
DGATLHTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTETDLSDEQTVTRWVNALVSELNDKE
QHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFITGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQ
ALDWLVKESRRGLSIQRYKGLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALKAAN
IDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=551, Percent_Identity=26.1343012704174, Blast_Score=132, Evalue=2e-30,
Organism=Homo sapiens, GI19913408, Length=571, Percent_Identity=24.6935201401051, Blast_Score=128, Evalue=2e-29,
Organism=Escherichia coli, GI48994957, Length=804, Percent_Identity=99.6268656716418, Blast_Score=1662, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=555, Percent_Identity=41.0810810810811, Blast_Score=392, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17535065, Length=542, Percent_Identity=26.7527675276753, Blast_Score=135, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI212645845, Length=522, Percent_Identity=26.0536398467433, Blast_Score=132, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI212645657, Length=153, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=589, Percent_Identity=25.9762308998302, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17136538, Length=527, Percent_Identity=25.9962049335863, Blast_Score=130, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 89994; Mature: 89863

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCKEII
CCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEE
VTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGV
EEEECCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
SVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGETEKTGTMVRFWPSLETFTNVT
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEECCCCCCCCEEEECCCHHHHHCHH
EFEYDILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHYEGGIKAFVEYLNKNKTPIHPNI
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCHHHHHHHHHCCCCCCCCCCE
FYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQRDGGTHLAGFRAAMTRTLNAYMDKE
EEEECCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
GYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSSQTKDKLVSSEVKSAVEQQMNELLAEY
CCCCCCEEECCCCHHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LLENPTDAKIVVGKIIDAARAREAARRAREMTRRKGALDLAGLPGKLADCQERDPALSEL
HHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCEE
YLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGR
EEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPEIVERGHVYIAQPPLYKVKKG
CCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHCC
KQEQYIKDDEAMDQYQISIALDGATLHTNASAPALAGEALEKLVSEYNATQKMINRMERR
CHHHHCCCCCCCCEEEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YPKAMLKELIYQPTLTETDLSDEQTVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLF
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCCCHHHHH
EPIVRVRTHGVDTDYPLDHEFITGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFE
HHHHHHHHCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
QALDWLVKESRRGLSIQRYKGLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTL
HHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHH
MGDAVEPRRAFIEENALKAANIDI
HCCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCKEII
CCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEE
VTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGV
EEEECCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHH
SVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGETEKTGTMVRFWPSLETFTNVT
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEECCCCCCCCEEEECCCHHHHHCHH
EFEYDILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHYEGGIKAFVEYLNKNKTPIHPNI
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEECCHHHHHHHHHCCCCCCCCCCE
FYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQRDGGTHLAGFRAAMTRTLNAYMDKE
EEEECCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
GYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSSQTKDKLVSSEVKSAVEQQMNELLAEY
CCCCCCEEECCCCHHHCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LLENPTDAKIVVGKIIDAARAREAARRAREMTRRKGALDLAGLPGKLADCQERDPALSEL
HHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCEE
YLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALGCGIGR
EEEECCCCCCCCCCCCCCCCCEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DEYNPDKLRYHNIIIMTDADVDGSHIRTLLLTFFYRQMPEIVERGHVYIAQPPLYKVKKG
CCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHCC
KQEQYIKDDEAMDQYQISIALDGATLHTNASAPALAGEALEKLVSEYNATQKMINRMERR
CHHHHCCCCCCCCEEEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YPKAMLKELIYQPTLTETDLSDEQTVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLF
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCCCHHHHH
EPIVRVRTHGVDTDYPLDHEFITGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFE
HHHHHHHHCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
QALDWLVKESRRGLSIQRYKGLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTL
HHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHH
MGDAVEPRRAFIEENALKAANIDI
HCCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]