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The map label for this gene is livH [H]

Identifier: 170680877

GI number: 170680877

Start: 3806725

End: 3807651

Strand: Reverse

Name: livH [H]

Synonym: EcSMS35_3739

Alternate gene names: 170680877

Gene position: 3807651-3806725 (Counterclockwise)

Preceding gene: 170683091

Following gene: 170681266

Centisome position: 75.13

GC content: 55.77

Gene sequence:

>927_bases
ATGTCTGAGCAGTTTTTGTATTTCTTGCAGCAGATGTTTAACGGCGTCACGCTGGGCAGTACCTACGCGCTGATAGCCAT
CGGCTACACCATGGTTTACGGCATTATCGGCATGATCAACTTCGCCCACGGCGAGGTTTACATGATTGGCAGCTACGTCT
CCTTTATGATCATCGCTGCACTGATGATGCTGGGAATTGATACCGGCTGGCTGCTGGTGGCGGCGGGATTCGTTGGCGCA
ATCGTCATTGCCAGCGCCTACGGCTGGAGTATCGAACGGGTGGCCTATCGCCCGGTGCGTAACTCTAAGCGCCTGATTGC
GCTTATTTCCGCAATCGGCATGTCTATCTTCCTGCAAAACTACGTCAGCCTGACCGAAGGTTCGCGCGACGTGGCGCTGC
CGAGCCTGTTTAACGGTCAGTGGGTGGTAGGCCATAGCGAAAACTTCTCTGCCTCTATTACTACCATGCAGGCGGTTATC
TGGATTGTTACTTTCCTCGCCATGCTGGCGCTGACGATTTTCATTCGCTATTCCCGTATGGGGCGAGCCTGCCGTGCCTG
CGCGGAAGATCTGAAAATGGCGAGCCTGCTTGGCATCAACACCGACCGGGTGATTGCGCTGACCTTTGTGATTGGCGCGG
CGATGGCGGCGGTGGCGGGTGTGCTGCTCGGTCAGTTCTACGGCGTAATTAACCCCTACATCGGCTTTATGGCCGGGATG
AAAGCCTTTACCGCTGCGGTGCTCGGTGGGATTGGCAGCATTCCGGGAGCGATGATTGGCGGCCTGATTCTGGGGATTGC
GGAGGCGCTCTCTTCTGCCTATCTGAGTACGGAATATAAAGATGTGGTCTCATTCGCCCTGCTGATTCTGGTGTTGCTGG
TGATGCCGACCGGTATTCTGGGTCGCCCGGAGGTAGAGAAAGTATGA

Upstream 100 bases:

>100_bases
ATTTTGGTGTCTTCCAGTGGCACGCCGACGGTTCATCCACGGCAGCCAAGTGATCATCCCACTGCCCGTGAAAAACGGGC
GGGTTTAGAAAGGTTACCTT

Downstream 100 bases:

>100_bases
AACCGATGCATATTGCAATGGCGCTGCTCTCTGCCGCCATGTTCTTTGTGCTGGCGGGCGTCTTTATGGGCGTGCAACTG
GAGCTGGATGGCACCAAACT

Product: branched-chain amino acid transporter permease subunit LivH

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein H [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHGEVYMIGSYVSFMIIAALMMLGIDTGWLLVAAGFVGA
IVIASAYGWSIERVAYRPVRNSKRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAVAGVLLGQFYGVINPYIGFMAGM
KAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLSTEYKDVVSFALLILVLLVMPTGILGRPEVEKV

Sequences:

>Translated_308_residues
MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHGEVYMIGSYVSFMIIAALMMLGIDTGWLLVAAGFVGA
IVIASAYGWSIERVAYRPVRNSKRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAVAGVLLGQFYGVINPYIGFMAGM
KAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLSTEYKDVVSFALLILVLLVMPTGILGRPEVEKV
>Mature_307_residues
SEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHGEVYMIGSYVSFMIIAALMMLGIDTGWLLVAAGFVGAI
VIASAYGWSIERVAYRPVRNSKRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVIW
IVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAVAGVLLGQFYGVINPYIGFMAGMK
AFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLSTEYKDVVSFALLILVLLVMPTGILGRPEVEKV

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=308, Percent_Identity=99.6753246753247, Blast_Score=607, Evalue=1e-175,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 32965; Mature: 32834

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHGEVYMIGSYVSFMIIAA
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
LMMLGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNSKRLIALISAIGMSIFLQN
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
YVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVIWIVTFLAMLALTIFIRYSRM
HHHHHCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAVAGVLLGQFYGVINPYIGFMAGM
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLSTEYKDVVSFALLILVLLVMPTGIL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GRPEVEKV
CCCCCCCC
>Mature Secondary Structure 
SEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHGEVYMIGSYVSFMIIAA
CHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
LMMLGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNSKRLIALISAIGMSIFLQN
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
YVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVIWIVTFLAMLALTIFIRYSRM
HHHHHCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAVAGVLLGQFYGVINPYIGFMAGM
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLSTEYKDVVSFALLILVLLVMPTGIL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GRPEVEKV
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]