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The map label for this gene is glnE [H]

Identifier: 170680866

GI number: 170680866

Start: 3422418

End: 3425258

Strand: Reverse

Name: glnE [H]

Synonym: EcSMS35_3345

Alternate gene names: 170680866

Gene position: 3425258-3422418 (Counterclockwise)

Preceding gene: 170682741

Following gene: 170683743

Centisome position: 67.58

GC content: 55.76

Gene sequence:

>2841_bases
ATGAAGCCGCTCTCTTCACCGTTACAGCAGTACTGGCAGACCGTTGTTGAGCGGCTGCCAGAGCCTTTAGCCGACGAATC
ACTTAGCGCACAGGCGAAGTCGGTACTTACTTTCAGTGATTTTGTGCAGGACAGCGTGATTGCGCATCCAGAGTGGCTGA
CGGAACTGGAAAGCCAACCGCCGCAGGCCGACGAATGGCAGCATTACTCTGCATGGTGGCAGGAGGCGCTCAGTAATGTG
AGTGACGAAGCCGGGTTAATGCGCGAGCTGCGGCTATTCCGGCGGCGCATTATGGTGCGCATCGCCTGGGCGCAAACGTT
GGCACTGGTTACTGAAGAGAGCATATTGCTGCAGCTCAGCCATCTGGCGGAGACGCTGATTGTTGCGGCGCGTGACTGGC
TGTATGACGCCTGCTGCCGCGAGTGGGGAACGCCGTGCAATGCGCAGGGCGAAGCGCAACCGCTGCTGATTTTAGGCATG
GGTAAGCTGGGCGGAGGGGAACTGAATTTCTCCTCAGATATCGATCTGATTTTTGCCTGGCCGGAACATGGTTGTACGCA
GGGCGGACGCCGGGAACTGGATAACGCCCAGTTTTTTACCCGCATGGGGCAGCGGCTGATTAAAGTGCTGGATCAACCAA
CGCAGGATGGCTTCGTCTATCGCGTGGATATGCGACTGCGTCCGTTTGGCGAAAGTGGCCCGCTGGTGCTGAGCTTTGCG
GCACTGGAAGATTATTACCAGGAGCAGGGGCGCGACTGGGAGCGTTACGCGATGGTCAAGGCGCGGATTATGGGCGATAG
CGAAGGCGTCTATGCTAACGAGTTGCGTGCGATGCTGCGCCCGTTTGTTTTCCGTCGTTACATCGATTTCAGCGTGATTC
AGTCGCTGCGCAACATGAAAGGGATGATTGCCCGTGAAGTGCGTCGGCGCGGTTTGACCGACAACATCAAACTTGGCGCG
GGTGGCATTCGTGAAATTGAATTTATCGTCCAGGTATTCCAGCTCATTCGCGGCGGGCGCGAACCGTCGCTGCAATCGCG
CTCTTTACTGCCAACGCTCAGCGCCATTGCCGAGCTGCATCTGCTTTCTGAAAACGATGCTGAACAATTGCGAGTGGCGT
ATCTGTTCCTGCGGCGTCTGGAAAACCTGCTGCAAAGCATTAACGACGAACAAACCCAGACGCTTCCTTCTGATGAGCTT
AACCGTGCGCGGCTGGCGTGGGCGATGGACTTTGCTGACTGGCAGCAACTGACTGGGGCGCTGATCGGACATATGACCAA
TGTGCGCCGGGTGTTTAATGAATTGATTGGCGACGACGAAAGTGAAACTCAGGAAGAGTCGCTGTCGGAACAGTGGCGTG
AGCTGTGGCAGGACGCGTTGCAGGAAGATGACACTACACCAGTGCTGGCGCATCTTAGCGAGGACGATCGCAAACAGGTG
CTGACGCTGATTGCCGATTTCCGCAAAGAGCTGGATAAGCGCACAATCGGGCCACGCGGACGTCAGGTGCTCGACCATCT
GATGCCACATCTGCTAAGAGATGTCTGTACACGTGAAGACGCTGCTGTAACGCTGTCGCGCATTACGGCCTTGCTGGTGG
GGATTGTTACCCGCACCACCTATTTAGAATTGCTCAGTGAATTTCCTGCGGCGCTTAAGCATTTGATTTCTCTGTGTGCC
GCATCGCCGATGATTGCCAGCCAGCTGGCGCGCTATCCGTTATTGCTGGATGAACTGCTCGATCCAAACACTCTCTACCA
GCCGACGGCGACCGATGCCTACCGTGATGAACTGCGCCAGTATTTGCTGCGCGTGCCGGAAGATGACGAAGAACAACAGC
TTGAGGCGCTGCGTCAGTTCAAACAGGCGCAACTGTTACGCATTGCGGCAGCGGATATCGCCGGTACGCTACCGGTAATG
AAAGTGAGCGATCACTTAACCTGGCTGGCGGAAGCTATGATAGATGCCGTCGTTCAGCAGGCGTGGGTTCAAATGGTTGC
CCGCTACGGTAAACCGAATCACCTGAACGAGCGCGAAGGGCGTGGTTTTGCGGTGGTCGGCTACGGCAAGCTGGGCGGCT
GGGAGTTAGGCTACAGTTCCGATCTTGACCTGATCTTCCTCCATGATTGCCCAATGGATGCGATGACTGACGGCGAGCGG
GAAATCGACGGGCGACAGTTTTATCTGCGTCTGGCGCAACGCATTATGCATCTGTTCAGTACGCGAACCTCTTCCGGCAT
TTTGTATGAAGTGGATGCTCGACTGCGTCCATCCGGGGCGGCGGGAATGCTGGTGACATCCGCAGAAGCATTTGCTGATT
ATCAGAAAAACGAGGCCTGGACGTGGGAACATCAGGCGCTGGTGCGTGCGCGTGTAGTGTACGGCGATCCGCAGCTCACC
GCGCACTTTGACGCGGTGCGTCGCGAGATTATGACGCTGCCGCGAGAAGGTAAAACTCTGCAAACGGAAGTGCGGGAAAT
GCGCGAGAAAATGCGCGCTCATCTCGGTAATAAACATCGCGATCGCTTTGATATCAAAGCCGATGAAGGCGGAATTACCG
ATATCGAATTTATTACCCAATATCTGGTGTTGCGCTACGCTCATGAAAAACCGAAGTTAACGCGCTGGTCAGACAACGTA
CGTATTCTGGAACTGCTGGCGCAAAACGACATTATGGAAGAGCAGGAAGCGATGGCGCTGACCCGTGCTTACACTACGCT
GCGCGATGAACTTCATCATCTGGCATTACAGGAATTGCCGGGCCATGTGCCGGAGGATTGCTTCACCGCAGAGCGTGAAC
AGGTGCGGGTAAGCTGGCAGAAGTGGCTGGTGGAAGAATGA

Upstream 100 bases:

>100_bases
CAACGCATTGAAATTGAACATTTCCGTAATGAGGCAAACAATCAGGAACCGTTCTGGTTGCACAGCGGAAAACGTTAATC
AGGCAAGGATAAAATCGCTT

Downstream 100 bases:

>100_bases
AGTATGGTATTATCGCGCGCAAATTTTGAATCTCTCAGGAGACAGGAATGAAAGTAACGCTGCCAGAGTTTGAACGTGCA
GGAGTGATGGTGGTTGGTGA

Product: bifunctional glutamine-synthetase adenylyltransferase/deadenyltransferase

Products: NA

Alternate protein names: Glutamine-synthetase adenylyltransferase; ATase; [Glutamate--ammonia-ligase] adenylyltransferase [H]

Number of amino acids: Translated: 946; Mature: 946

Protein sequence:

>946_residues
MKPLSSPLQQYWQTVVERLPEPLADESLSAQAKSVLTFSDFVQDSVIAHPEWLTELESQPPQADEWQHYSAWWQEALSNV
SDEAGLMRELRLFRRRIMVRIAWAQTLALVTEESILLQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGFVYRVDMRLRPFGESGPLVLSFA
ALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELRAMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGA
GGIREIEFIVQVFQLIRGGREPSLQSRSLLPTLSAIAELHLLSENDAEQLRVAYLFLRRLENLLQSINDEQTQTLPSDEL
NRARLAWAMDFADWQQLTGALIGHMTNVRRVFNELIGDDESETQEESLSEQWRELWQDALQEDDTTPVLAHLSEDDRKQV
LTLIADFRKELDKRTIGPRGRQVLDHLMPHLLRDVCTREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCA
ASPMIASQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQLLRIAAADIAGTLPVM
KVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRGFAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGER
EIDGRQFYLRLAQRIMHLFSTRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQLT
AHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIEFITQYLVLRYAHEKPKLTRWSDNV
RILELLAQNDIMEEQEAMALTRAYTTLRDELHHLALQELPGHVPEDCFTAEREQVRVSWQKWLVEE

Sequences:

>Translated_946_residues
MKPLSSPLQQYWQTVVERLPEPLADESLSAQAKSVLTFSDFVQDSVIAHPEWLTELESQPPQADEWQHYSAWWQEALSNV
SDEAGLMRELRLFRRRIMVRIAWAQTLALVTEESILLQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGFVYRVDMRLRPFGESGPLVLSFA
ALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELRAMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGA
GGIREIEFIVQVFQLIRGGREPSLQSRSLLPTLSAIAELHLLSENDAEQLRVAYLFLRRLENLLQSINDEQTQTLPSDEL
NRARLAWAMDFADWQQLTGALIGHMTNVRRVFNELIGDDESETQEESLSEQWRELWQDALQEDDTTPVLAHLSEDDRKQV
LTLIADFRKELDKRTIGPRGRQVLDHLMPHLLRDVCTREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCA
ASPMIASQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQLLRIAAADIAGTLPVM
KVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRGFAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGER
EIDGRQFYLRLAQRIMHLFSTRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQLT
AHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIEFITQYLVLRYAHEKPKLTRWSDNV
RILELLAQNDIMEEQEAMALTRAYTTLRDELHHLALQELPGHVPEDCFTAEREQVRVSWQKWLVEE
>Mature_946_residues
MKPLSSPLQQYWQTVVERLPEPLADESLSAQAKSVLTFSDFVQDSVIAHPEWLTELESQPPQADEWQHYSAWWQEALSNV
SDEAGLMRELRLFRRRIMVRIAWAQTLALVTEESILLQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGFVYRVDMRLRPFGESGPLVLSFA
ALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELRAMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGA
GGIREIEFIVQVFQLIRGGREPSLQSRSLLPTLSAIAELHLLSENDAEQLRVAYLFLRRLENLLQSINDEQTQTLPSDEL
NRARLAWAMDFADWQQLTGALIGHMTNVRRVFNELIGDDESETQEESLSEQWRELWQDALQEDDTTPVLAHLSEDDRKQV
LTLIADFRKELDKRTIGPRGRQVLDHLMPHLLRDVCTREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCA
ASPMIASQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQLLRIAAADIAGTLPVM
KVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRGFAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGER
EIDGRQFYLRLAQRIMHLFSTRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQLT
AHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIEFITQYLVLRYAHEKPKLTRWSDNV
RILELLAQNDIMEEQEAMALTRAYTTLRDELHHLALQELPGHVPEDCFTAEREQVRVSWQKWLVEE

Specific function: Adenylation and deadenylation of glutamate--ammonia ligase [H]

COG id: COG1391

COG function: function code OT; Glutamine synthetase adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glnE family [H]

Homologues:

Organism=Escherichia coli, GI1789433, Length=946, Percent_Identity=98.5200845665962, Blast_Score=1903, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023057
- InterPro:   IPR005190
- InterPro:   IPR013546 [H]

Pfam domain/function: PF08335 GlnD_UR_UTase; PF03710 GlnE [H]

EC number: =2.7.7.42 [H]

Molecular weight: Translated: 108619; Mature: 108619

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLSSPLQQYWQTVVERLPEPLADESLSAQAKSVLTFSDFVQDSVIAHPEWLTELESQP
CCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
PQADEWQHYSAWWQEALSNVSDEAGLMRELRLFRRRIMVRIAWAQTLALVTEESILLQLS
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGMGKLGGGELNFSSDIDLIFAW
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEC
PEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGFVYRVDMRLRPFGESGPLVLSFA
CCCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCEEEEHH
ALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELRAMLRPFVFRRYIDFSVIQSLRNMK
HHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQLIRGGREPSLQSRSLLPTLSAIAELH
HHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LLSENDAEQLRVAYLFLRRLENLLQSINDEQTQTLPSDELNRARLAWAMDFADWQQLTGA
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LIGHMTNVRRVFNELIGDDESETQEESLSEQWRELWQDALQEDDTTPVLAHLSEDDRKQV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHH
LTLIADFRKELDKRTIGPRGRQVLDHLMPHLLRDVCTREDAAVTLSRITALLVGIVTRTT
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YLELLSEFPAALKHLISLCAASPMIASQLARYPLLLDELLDPNTLYQPTATDAYRDELRQ
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
YLLRVPEDDEEQQLEALRQFKQAQLLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQ
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
AWVQMVARYGKPNHLNEREGRGFAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGER
HHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCHHHCCCCH
EIDGRQFYLRLAQRIMHLFSTRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAW
HCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEECHHHHHHHHCCCCC
TWEHQALVRARVVYGDPQLTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHR
CHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCC
DRFDIKADEGGITDIEFITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMAL
CCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TRAYTTLRDELHHLALQELPGHVPEDCFTAEREQVRVSWQKWLVEE
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPLSSPLQQYWQTVVERLPEPLADESLSAQAKSVLTFSDFVQDSVIAHPEWLTELESQP
CCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
PQADEWQHYSAWWQEALSNVSDEAGLMRELRLFRRRIMVRIAWAQTLALVTEESILLQLS
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGMGKLGGGELNFSSDIDLIFAW
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEC
PEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGFVYRVDMRLRPFGESGPLVLSFA
CCCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCEEEEHH
ALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELRAMLRPFVFRRYIDFSVIQSLRNMK
HHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQLIRGGREPSLQSRSLLPTLSAIAELH
HHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
LLSENDAEQLRVAYLFLRRLENLLQSINDEQTQTLPSDELNRARLAWAMDFADWQQLTGA
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LIGHMTNVRRVFNELIGDDESETQEESLSEQWRELWQDALQEDDTTPVLAHLSEDDRKQV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHH
LTLIADFRKELDKRTIGPRGRQVLDHLMPHLLRDVCTREDAAVTLSRITALLVGIVTRTT
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YLELLSEFPAALKHLISLCAASPMIASQLARYPLLLDELLDPNTLYQPTATDAYRDELRQ
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
YLLRVPEDDEEQQLEALRQFKQAQLLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQ
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
AWVQMVARYGKPNHLNEREGRGFAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGER
HHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCHHHCCCCH
EIDGRQFYLRLAQRIMHLFSTRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAW
HCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEECHHHHHHHHCCCCC
TWEHQALVRARVVYGDPQLTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHR
CHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCC
DRFDIKADEGGITDIEFITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMAL
CCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TRAYTTLRDELHHLALQELPGHVPEDCFTAEREQVRVSWQKWLVEE
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]