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The map label for this gene is hyaD [H]

Identifier: 170680751

GI number: 170680751

Start: 2151224

End: 2151811

Strand: Reverse

Name: hyaD [H]

Synonym: EcSMS35_2142

Alternate gene names: 170680751

Gene position: 2151811-2151224 (Counterclockwise)

Preceding gene: 170682734

Following gene: 170681401

Centisome position: 42.46

GC content: 58.16

Gene sequence:

>588_bases
ATGAGCGAGCAACGCGTGGTGGTAATGGGGCTGGGCAACCTGCTGTGGGCCGATGAAGGTTTCGGCGTGCGGGTGGCGGA
ACGGTTGTATGCCCATTACCACTGGCCCGAGTATGTGGAGATTGTCGATGGCGGTATTCAGGGACTGAACTTGCTGGGGT
ATGTCGAAAGCGCCAGCCATCTGTTGATTCTCGATGCCATTGACTACGGGCTGGAACCTGGAACGCTGCGAACCTATGCC
GGAGAACGTATTCCGGCTTATCTCAGCGCGAAGAAAATGAGCCTGCACCAGAACAGTTTCTCCGAAGTGCTGGCGCTGGC
AGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCAATGCTCGACGACTACGGCGGCAGCC
TGAGCGAACTGGCACGGGAGCAACTGCCCGCAGCGGAACAGGCGGCGCTGGCGCAGTTGGCTGCGTGGGGAATTGTGCCG
CAACCGGCTAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTA
CAGGATGACGCTGGAGGAGCAGGAATGA

Upstream 100 bases:

>100_bases
CGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACTATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAA
ATAAGTAACAAGGAGCGTTC

Downstream 100 bases:

>100_bases
GCAACGACACGCCATTTGATGCGTTGTGGCAACGTATGCTGGCGCGCGGCTGGACGCCGATCAGTGAATCCCGTCTTGAC
GACTGGCTTACGCAAGCGCC

Product: hydrogenase 1 maturation protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDGGIQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYA
GERIPAYLSAKKMSLHQNSFSEVLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQE

Sequences:

>Translated_195_residues
MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDGGIQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYA
GERIPAYLSAKKMSLHQNSFSEVLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQE
>Mature_194_residues
SEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDGGIQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAG
ERIPAYLSAKKMSLHQNSFSEVLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVPQ
PANESRCLNYDCLSMENYEGVRLRQYRMTLEEQE

Specific function: Protease involved in the C-terminal processing of hyaB, the large subunit of hydrogenase 1 (Potential) [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1787209, Length=194, Percent_Identity=98.9690721649485, Blast_Score=389, Evalue=1e-110,
Organism=Escherichia coli, GI1789367, Length=155, Percent_Identity=38.7096774193548, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 21616; Mature: 21485

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDGGIQGLNLLGYVESASH
CCCCEEEEEECCCEEECCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCHHHHEEEECCCCE
LLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSEVLALADIRGHLPAHIALV
EEEEEEHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
GLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVPQPANESRCLNYDCLSMENYE
ECCCHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCC
GVRLRQYRMTLEEQE
CCEEEEEECHHCCCC
>Mature Secondary Structure 
SEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDGGIQGLNLLGYVESASH
CCCEEEEEECCCEEECCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCHHHHEEEECCCCE
LLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSEVLALADIRGHLPAHIALV
EEEEEEHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
GLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVPQPANESRCLNYDCLSMENYE
ECCCHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCC
GVRLRQYRMTLEEQE
CCEEEEEECHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ni [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2180913; 8905232; 9278503 [H]