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The map label for this gene is citT [H]

Identifier: 170680678

GI number: 170680678

Start: 644517

End: 645947

Strand: Reverse

Name: citT [H]

Synonym: EcSMS35_0631

Alternate gene names: 170680678

Gene position: 645947-644517 (Counterclockwise)

Preceding gene: 170683009

Following gene: 170679706

Centisome position: 12.74

GC content: 53.39

Gene sequence:

>1431_bases
TTGGCGCCCCTGGTGGTGATGGGTGTCATGTTTCTTATCCCTGTACCTGACGGTATGCCGCCGCAGGCGTGGCATTACTT
TGCAGTGTTTGTGGCAATGATTGTCGGCATGATCCTCGAGCCAATTCCGGCAACGGCGATCAGTTTTATTGCGGTTACTA
TTTGCGTTATTGGCAGTAATTACCTGCTCTTTGATGCCAAAGAATTAGCTGACCCAGCGTTTAATGCGCAAAAACAGGCG
CTGAAATGGGGGCTGGCAGGTTTCTCCAGCACCACCGTCTGGCTGGTGTTTGGCGCATTTATTTTTGCGCTGGGCTATGA
AGTCACCGGTCTGGGCCGTCGTATCGCCCTTTTCCTGGTGAAATTCATGGGTAAACGCACGCTGACGCTGGGTTACGCGA
TTGTCATTATCGACATTCTGCTGGCACCGTTTACACCGTCCAACACCGCGCGTACCGGGGGTACGGTTTTTCCGGTCATT
AAAAACCTGCCGCCGCTGTTTAAATCATTCCCGAACGATCCGTCCGCGCGTCGTATTGGCGGCTATTTGATGTGGATGAT
GGTCATTAGTACCAGTCTGAGTTCGTCCATGTTTGTCACCGGTGCGGCACCAAACGTGCTGGGTCTGGAGTTCGTCAGCA
AAATCGCAGGGATCCAGATTAGCTGGCTGCAATGGTTCCTGAGCTTCCTGCCGGTCGGTATTATTTTGCTGATCGTTGCT
CCGTGGCTCTCCTATGTGCTGTACAAGCCGGAAGTGACTCACAGTGCCGAAGTGGCAGCATGGGCCGGTGATGAACTGAA
AACGATGGGTGCATTGAGCCGTAAAGAGTGGACCCTGATAGGTCTGGTGCTGCTGAGCTTAGGCTTATGGGTATTCGGCG
GCGAAATGATCGACGCCACGGCGGTAGGTCTGCTGGCGGTTTCGCTGATGCTGGCCCTGCACGTTGTACCGTGGAAAGAC
ATTACCCGCTACAACAGCGCCTGGAACACACTGGTCAACCTGGCAACGCTGGTTGTTATGGCGAACGGTTTGACCCGCTC
TGGTTTTATCGACTGGTTCGCTAGCACCATGAGCACGCACCTGGAAGGCTTCTCACCGAACGCAACGGTAATTGTACTGG
TTCTGGTGTTCTACTTTGCACACTACCTGTTTGCCAGCCTGTCTGCGCACACCGCAACCATGCTGCCGGTTATTCTGGCC
GTCGGTAAAGGTATTCCGGGCGTACCAATGGAACAACTGTGTATCCTGCTGGTGCTGTCTATCGGTATCATGGGCTGTCT
GACGCCGTATGCAACCGGTCCTGGGGTGATTATTTACGGCTGTGGCTATGTGAAATCAAAAGATTACTGGCGTCTTGGCG
CAATCTTCGGGGTGATTTACATCTCTATGCTGCTGTTGGTTGGCTGGCCGATTCTCGCCATGTGGAACTAA

Upstream 100 bases:

>100_bases
TTTTTAGCACAGATTTAATAATTTAAGCACTTGATAAAATTGGAATATTAATTGTCGGAGAACCCGTATGTCTTTAGCAA
AAGATAATATATGGAAATTA

Downstream 100 bases:

>100_bases
TACGTTCGCCAAACGCTGAATAAATCATCACGCCCGCCAGGTAAGCCCACCTGGCGGGCTTTTTTATGATTTAATAGATA
GTATTCAGTATATGTATGAG

Product: sodium:sulfate symporter family protein

Products: NA

Alternate protein names: Citrate transporter; Citrate/succinate antiporter [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQA
LKWGLAGFSSTTVWLVFGAFIFALGYEVTGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNTARTGGTVFPVI
KNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQISWLQWFLSFLPVGIILLIVA
PWLSYVLYKPEVTHSAEVAAWAGDELKTMGALSRKEWTLIGLVLLSLGLWVFGGEMIDATAVGLLAVSLMLALHVVPWKD
ITRYNSAWNTLVNLATLVVMANGLTRSGFIDWFASTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSAHTATMLPVILA
VGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN

Sequences:

>Translated_476_residues
MAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQA
LKWGLAGFSSTTVWLVFGAFIFALGYEVTGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNTARTGGTVFPVI
KNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQISWLQWFLSFLPVGIILLIVA
PWLSYVLYKPEVTHSAEVAAWAGDELKTMGALSRKEWTLIGLVLLSLGLWVFGGEMIDATAVGLLAVSLMLALHVVPWKD
ITRYNSAWNTLVNLATLVVMANGLTRSGFIDWFASTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSAHTATMLPVILA
VGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN
>Mature_475_residues
APLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQAL
KWGLAGFSSTTVWLVFGAFIFALGYEVTGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNTARTGGTVFPVIK
NLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQISWLQWFLSFLPVGIILLIVAP
WLSYVLYKPEVTHSAEVAAWAGDELKTMGALSRKEWTLIGLVLLSLGLWVFGGEMIDATAVGLLAVSLMLALHVVPWKDI
TRYNSAWNTLVNLATLVVMANGLTRSGFIDWFASTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSAHTATMLPVILAV
GKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN

Specific function: Responsible for the uptake of citrate in exchange to the efflux of succinate. Has a relatively broad specificity for C(4)- dicarboxylates and tricarboxylates [H]

COG id: COG0471

COG function: function code P; Di- and tricarboxylate transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SLC13A transporter (TC 2.A.47) family. SODIT1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786829, Length=476, Percent_Identity=97.0588235294118, Blast_Score=925, Evalue=0.0,
Organism=Escherichia coli, GI1789444, Length=468, Percent_Identity=45.2991452991453, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1786986, Length=473, Percent_Identity=34.46088794926, Blast_Score=250, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001898 [H]

Pfam domain/function: PF00939 Na_sulph_symp [H]

EC number: NA

Molecular weight: Translated: 51698; Mature: 51566

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISFIAVTICVIGSN
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
YLLFDAKELADPAFNAQKQALKWGLAGFSSTTVWLVFGAFIFALGYEVTGLGRRIALFLV
EEEEEHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHH
KFMGKRTLTLGYAIVIIDILLAPFTPSNTARTGGTVFPVIKNLPPLFKSFPNDPSARRIG
HHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHCCCHHHHCCCCCCCHHHHH
GYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQISWLQWFLSFLPVGIILLIVA
HHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
PWLSYVLYKPEVTHSAEVAAWAGDELKTMGALSRKEWTLIGLVLLSLGLWVFGGEMIDAT
HHHHHHCCCCCCCCCCCHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
AVGLLAVSLMLALHVVPWKDITRYNSAWNTLVNLATLVVMANGLTRSGFIDWFASTMSTH
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LEGFSPNATVIVLVLVFYFAHYLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLS
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
IGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN
HHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure 
APLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISFIAVTICVIGSN
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
YLLFDAKELADPAFNAQKQALKWGLAGFSSTTVWLVFGAFIFALGYEVTGLGRRIALFLV
EEEEEHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHH
KFMGKRTLTLGYAIVIIDILLAPFTPSNTARTGGTVFPVIKNLPPLFKSFPNDPSARRIG
HHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHCCCHHHHCCCCCCCHHHHH
GYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQISWLQWFLSFLPVGIILLIVA
HHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
PWLSYVLYKPEVTHSAEVAAWAGDELKTMGALSRKEWTLIGLVLLSLGLWVFGGEMIDAT
HHHHHHCCCCCCCCCCCHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
AVGLLAVSLMLALHVVPWKDITRYNSAWNTLVNLATLVVMANGLTRSGFIDWFASTMSTH
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LEGFSPNATVIVLVLVFYFAHYLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLS
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
IGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN
HHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]