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The map label for this gene is hisH [H]

Identifier: 170680632

GI number: 170680632

Start: 1057975

End: 1058565

Strand: Reverse

Name: hisH [H]

Synonym: EcSMS35_1037

Alternate gene names: 170680632

Gene position: 1058565-1057975 (Counterclockwise)

Preceding gene: 170683419

Following gene: 170680218

Centisome position: 20.89

GC content: 57.36

Gene sequence:

>591_bases
ATGAACGTGGTGATCCTTGATACCGGCTGCGCCAATCTGAACTCGGTGAAGTCTGCCATTGCGCGCCACGGTTATGAACC
CAAAGTCAGCCGTGACCCGGACGTCGTGTTGCTGGCCGATAAACTGTTTTTACCCGGCGTTGGCACCGCGCAAGCGGCGA
TGGACCAGGTGCGTGAGCGCGAGCTGTTTGATCTCATCAAAGCCTGTACCCAACCGGTGCTGGGCATCTGCTTAGGGATG
CAACTGCTGGGGCGGCGCAGCGAAGAGAGCAACGGCATCGACTTGCTGGGCATCATCGACGAAGACGTGCCGAAAATGAC
CGACTTTGGTCTGCCGCTGCCGCATATGGGCTGGAACCGCGTTTACCCGCAGGCAGGCAACCGCCTGTTTCAGGGGATTG
AAGACGGCGCGTACTTTTACTTTGTTCACAGCTACGCAATGCCAGTCAATCCGTGGACCATCGCCCAGTGTAACTACGGC
GAACCGTTCACCGCGGCGGTACAAAAAGATAACTTCTATGGCGTGCAGTTCCACCCGGAGCGTTCTGGTGCCGCTGGCGC
TAAGTTGCTGAAAAACTTCCTGGAGATGTGA

Upstream 100 bases:

>100_bases
ATCACCGTGTAGAGAGCCTGTTCAAAGCCTTTGGTCGCACCCTGCGCCAGGCCATCCGCGTGGAAGGCGACACCCTGCCC
TCGTCAAAAGGAGTGCTGTA

Downstream 100 bases:

>100_bases
TGATTATTCCGGCATTAGATTTAATCGACGGCACCGTGGTGCGTCTCCATCAGGGCGATTATGGCAAACAACGCGATTAC
GGTAACGACCCGCTGCCACG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFLPGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGM
QLLGRRSEESNGIDLLGIIDEDVPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM

Sequences:

>Translated_196_residues
MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFLPGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGM
QLLGRRSEESNGIDLLGIIDEDVPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM
>Mature_196_residues
MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFLPGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGM
QLLGRRSEESNGIDLLGIIDEDVPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=196, Percent_Identity=99.4897959183673, Blast_Score=409, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6319725, Length=209, Percent_Identity=35.4066985645933, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 21667; Mature: 21667

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00442 GATASE_TYPE_I ; PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFLPGVGTAQAAMDQVRER
CEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCEECCCCCHHHHHHHHHHHH
ELFDLIKACTQPVLGICLGMQLLGRRSEESNGIDLLGIIDEDVPKMTDFGLPLPHMGWNR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCC
VYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYGEPFTAAVQKDNFYGVQFHPE
CCCHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEEECCCCCCEEEEEECCCEEEEEECCC
RSGAAGAKLLKNFLEM
CCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFLPGVGTAQAAMDQVRER
CEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCEECCCCCHHHHHHHHHHHH
ELFDLIKACTQPVLGICLGMQLLGRRSEESNGIDLLGIIDEDVPKMTDFGLPLPHMGWNR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCC
VYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYGEPFTAAVQKDNFYGVQFHPE
CCCHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEEECCCCCCEEEEEECCCEEEEEECCC
RSGAAGAKLLKNFLEM
CCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10222209; 11206551; 11258796 [H]