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The map label for this gene is purH [H]

Identifier: 170680627

GI number: 170680627

Start: 4550447

End: 4552036

Strand: Reverse

Name: purH [H]

Synonym: EcSMS35_4455

Alternate gene names: 170680627

Gene position: 4552036-4550447 (Counterclockwise)

Preceding gene: 170683355

Following gene: 170683731

Centisome position: 89.81

GC content: 55.66

Gene sequence:

>1590_bases
ATGCAACAACGTCGTCCAGTCCGCCGCGCTCTGCTCAGTGTTTCTGACAAAGCCGGTATCGTCGAATTCGCCCAGGCACT
TTCCGCACGCGGTGTGGAGCTGCTGTCTACAGGGGGCACTGCCCGTCTGTTAGCAGAAAAAGGTCTGCCGGTAACCGAAG
TTTCCGATTACACCGGTTTCCCGGAGATGATGGATGGACGCGTGAAGACCCTGCATCCGAAAGTACATGGTGGCATTCTG
GGCCGTCGCGGCCAGGACGATACCATTATGGAAGAACATCAGATCCAGCCTATCGATATGGTGGTTGTTAACCTGTATCC
GTTCGCCCAGACCGTTGCTCGTGAAGGCTGCTCGCTGGAAGATGCGGTTGAGAACATCGATATCGGCGGCCCGACGATGG
TGCGCTCTGCCGCCAAGAACCATAAAGATGTCGCCATCGTGGTGAAGAGCAGCGACTACGACGCCATTATTAAAGAGATA
GATGCCAATGAAGGCTCCCTGACTCTGGAAACTCGCTTTGACCTTGCCATCAAAGCCTTCGAACACACCGCCGCCTACGA
CAGCATGATTGCCAACTACTTCGGCAGCATGGTTCCGGCTTACCACGGTGAAAGCAAAGAAGCCGCCGGTCGCTTCCCAC
GCACGCTGAACCTGAACTTCATTAAGAAGCAGGATATGCGTTACGGCGAGAACAGCCACCAGCAGGCTGCCTTCTATATA
GAAGAGAATGTCAAAGAAGCCTCCGTTGCTACCGCAACCCAGGTTCAGGGTAAAGCCCTCTCTTATAACAACATCGCCGA
TACCGATGCGGCGCTGGAGTGCGTGAAAGAGTTCGCCGAGCCGGCATGTGTGATTGTGAAGCACGCCAACCCTTGCGGCG
TGGCTATCGGCAATTCCATTCTTGATGCTTACGATCGCGCGTACAAAACCGACCCGACCTCCGCATTCGGCGGCATTATC
GCCTTTAACCGCGAGCTGGATGCGGAAACCGCGCAGGCCATCATTTCTCGTCAGTTTGTTGAAGTGATTATTGCGCCGTC
CGCCAGCGAAGAAGCCCTGAAAATCACCGCCGCCAAACAGAACGTACGCGTCCTGACCTGCGGTCAGTGGGGCGAGCGTG
TTCCGGGTCTTGATTTCAAACGCGTGAACGGCGGTCTGCTGGTTCAGGATCGAGACCTGGGGATGGTCGGTGCAGAAGAA
CTGCGCGTCGTCACCAAACGTCAGCCGAGCGAACAGGAACTGCGTGATGCGCTGTTCTGCTGGAAAGTGGCGAAGTTCGT
GAAATCCAATGCTATCGTCTATGCCAAAAACAATATGACCATCGGTATTGGCGCAGGCCAGATGAGCCGTGTGTACTCCG
CGAAAATCGCCGGTATTAAAGCGGCCGATGAAGGCCTGGAAGTGAAAGGTTCCTCGATGGCTTCTGACGCATTCTTCCCG
TTCCGCGACGGTATTGATGCCGCCGCCGCTGCGGGTGTGACCTGTGTAATCCAGCCTGGCGGTTCTATCCGTGATGACGA
AGTAATTGCCGCCGCCGACGAGCACGGTATTGCAATGCTCTTCACCGACATGCGCCACTTCCGCCATTAA

Upstream 100 bases:

>100_bases
CGGGCGAAAAATAAGGATGCCCCGTTAGGGGCGTTAGCTGAGTTTTTCGCGAAAAATTCAGCTAACGCTCTCTGTAATAG
TCAAATCCAGGGGATTTACC

Downstream 100 bases:

>100_bases
TGGAGCAATAGATGAAAGTATTAGTGATTGGTAACGGCGGGCGCGAGCACGCGCTGGCCTGGAAAGCGGCCCAGTCGCCG
CTGGTTGAAACTGTTTTTGT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLLAEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRGQDDTIMEEHQIQPIDMVVVNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEI
DANEGSLTLETRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI
EENVKEASVATATQVQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGII
AFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRDLGMVGAEE
LRVVTKRQPSEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSSMASDAFFP
FRDGIDAAAAAGVTCVIQPGGSIRDDEVIAAADEHGIAMLFTDMRHFRH

Sequences:

>Translated_529_residues
MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLLAEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRGQDDTIMEEHQIQPIDMVVVNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEI
DANEGSLTLETRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI
EENVKEASVATATQVQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGII
AFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRDLGMVGAEE
LRVVTKRQPSEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSSMASDAFFP
FRDGIDAAAAAGVTCVIQPGGSIRDDEVIAAADEHGIAMLFTDMRHFRH
>Mature_529_residues
MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLLAEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRGQDDTIMEEHQIQPIDMVVVNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEI
DANEGSLTLETRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI
EENVKEASVATATQVQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGII
AFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRDLGMVGAEE
LRVVTKRQPSEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSSMASDAFFP
FRDGIDAAAAAGVTCVIQPGGSIRDDEVIAAADEHGIAMLFTDMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=485, Percent_Identity=39.3814432989691, Blast_Score=280, Evalue=2e-75,
Organism=Escherichia coli, GI1790439, Length=529, Percent_Identity=99.054820415879, Blast_Score=1080, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=614, Percent_Identity=33.8762214983713, Blast_Score=294, Evalue=6e-80,
Organism=Caenorhabditis elegans, GI71985574, Length=354, Percent_Identity=27.4011299435028, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71985556, Length=115, Percent_Identity=37.3913043478261, Blast_Score=76, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323056, Length=490, Percent_Identity=35.1020408163265, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6323768, Length=485, Percent_Identity=35.6701030927835, Blast_Score=266, Evalue=7e-72,
Organism=Drosophila melanogaster, GI24649832, Length=611, Percent_Identity=35.0245499181669, Blast_Score=306, Evalue=2e-83,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 57371; Mature: 57371

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLLAEKGLPVTEVSDYTGF
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCEEEHHHCCCCCEECCCCCCC
PEMMDGRVKTLHPKVHGGILGRRGQDDTIMEEHQIQPIDMVVVNLYPFAQTVAREGCSLE
HHHHCCCEEEECCHHHCCCCCCCCCCCCHHHHCCCCCEEEEEEEHHHHHHHHHHCCCCHH
DAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEIDANEGSLTLETRFDLAIKAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCCCEEEEEECCCHHHHH
EHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEEEEHHHCCCCCCCCCEEEEEE
EENVKEASVATATQVQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVAIGNSI
ECCCCHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHH
LDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQ
HHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHEEECCCCCCCEEEEEEECC
NVRVLTCGQWGERVPGLDFKRVNGGLLVQDRDLGMVGAEELRVVTKRQPSEQELRDALFC
CEEEEECCCCCCCCCCCCEEECCCCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHH
WKVAKFVKSNAIVYAKNNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSSMASDAFFP
HHHHHHHHCCEEEEEECCEEEEECCCHHHHHHHHHHCCCEECCCCCEECCCCCCCCCCCC
FRDGIDAAAAAGVTCVIQPGGSIRDDEVIAAADEHGIAMLFTDMRHFRH
CCCCCCHHHHCCEEEEECCCCCCCCCCEEEECCCCCEEEEEHHHHHHCC
>Mature Secondary Structure
MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLLAEKGLPVTEVSDYTGF
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCEEEHHHCCCCCEECCCCCCC
PEMMDGRVKTLHPKVHGGILGRRGQDDTIMEEHQIQPIDMVVVNLYPFAQTVAREGCSLE
HHHHCCCEEEECCHHHCCCCCCCCCCCCHHHHCCCCCEEEEEEEHHHHHHHHHHCCCCHH
DAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEIDANEGSLTLETRFDLAIKAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCCCEEEEEECCCHHHHH
EHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEEEEHHHCCCCCCCCCEEEEEE
EENVKEASVATATQVQGKALSYNNIADTDAALECVKEFAEPACVIVKHANPCGVAIGNSI
ECCCCHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHH
LDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQ
HHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHEEECCCCCCCEEEEEEECC
NVRVLTCGQWGERVPGLDFKRVNGGLLVQDRDLGMVGAEELRVVTKRQPSEQELRDALFC
CEEEEECCCCCCCCCCCCEEECCCCEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHH
WKVAKFVKSNAIVYAKNNMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSSMASDAFFP
HHHHHHHHCCEEEEEECCEEEEECCCHHHHHHHHHHCCCEECCCCCEECCCCCCCCCCCC
FRDGIDAAAAAGVTCVIQPGGSIRDDEVIAAADEHGIAMLFTDMRHFRH
CCCCCCHHHHCCEEEEECCCCCCCCCCEEEECCCCCEEEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA