Click here to switch to the map view.

The map label for this gene is menF [H]

Identifier: 170680455

GI number: 170680455

Start: 2468727

End: 2470022

Strand: Reverse

Name: menF [H]

Synonym: EcSMS35_2420

Alternate gene names: 170680455

Gene position: 2470022-2468727 (Counterclockwise)

Preceding gene: 170681985

Following gene: 170683335

Centisome position: 48.73

GC content: 53.32

Gene sequence:

>1296_bases
GTGCAATCACTTACTACGGCGCTGGAAAATCTACTGCGCCATTTGTCGCAAGAGATTCCGGCGACACCCGGCATTCGGGT
TATCGATATTCCTTTCCCTCTCAAAGACGCTTTTGATGCCTTGAACTGGCTGGCCAGTCAGCAGGTGTATCCGCAATTCT
ACTGGCAACAACGTAATGGTGATGAAGAAGCTGCCGTCCTGGGTGCGATAACCCGTTTTACGTCGTTGGACCAGGCACAA
CGTTTTCTTCGCCAGCACCCGGAACACGCCGACTTACGCATCTGGGGGTTGAATGCGTTTGACCCGTCGCAGGGCAATTT
ACTTTTACCACGCCTCGAATGGCGACGCTGTGGCGGTAAAGCCACGCTGCGGCTGACGCTATTCAGCGAAAGATCCCTTC
AGCATGATGCGATTAAGGCAAAAGAATTTATCGCCACACTGGTGAGTATTAAACCCTTGCCTGGGTTACATTTAACCACC
ACCCGAGAACAACACTGGCCGGACAAAACGGGCTGGACGCAATTAATCGAACTGGCAACGAAAACCATCGCCGAAGGCGA
GCTCGACAAAGTGGTGCTTGCTCGGGCAACTGACCTGCATTTCGCAAGTCCGGTCAATGCGGCGGCGATGATGGCTGCCA
GTCGTCGACTGAATCTGAATTGCTACCATTTTTACATGGCCTTTGATGGCGAAAATGCTTTTCTTGGCTCTTCACCGGAA
CGATTATGGTGGCGGCGTGACAAAGCGCTGCGTACTGAAGCGCTGGCGGGAACGGTAGCAAATCATCCTGATGATAAGCA
GGCGCAGCGGTTGGGTGAGTGGCTGATGGCGGATGATAAAAACCAGCGCGAGAACATGTTGGTGGTGGAGGATATCTGCC
AACGATTGCAGGCCGATACCCAGACGTTGGATGTTTTACCGCCGCAGGTACTGCGTCTGCGTAAAGTGCAGCATCTTCGC
CGCTGTATCTGGACTGCACTCAACAAAGCGGATGATGTGATCTGTTTACATCAGTTGCAGCCAACGGCAGCAGTTGCTGG
CTTACCGCGCGATCTGGCGCGACAGTTTATCGCCCGTCACGAACCGTTCACCCGAGAATGGTACGCCGGTTCTGCGGGCT
ATCTCTCATTACAACAAAGCGAATTCTGCGTTTCCCTGCGCTCAGCAAAAATTAGCGGCAATGTCGTGCGTCTATATGCT
GGCGCGGGCATTGTCCGTGGTTCCGACCCCGAGCAAGAGTGGCAGGAAATCGACAACAAAGCGGCAGGGCTGCGTACTTT
ATTACAAATGGAATAG

Upstream 100 bases:

>100_bases
ACTGTTGCTGGCACGCCGTTAAAACCTCCCTACACCGGGGTACTGCATTTTTTTTGTCGTACCCCGTATAATGTGAGGCT
TTTAACAGGGAGAGGTCCGC

Downstream 100 bases:

>100_bases
TAATGAGTCGCATCATTACCGATTCATATCAATAATCTATTTTTGTAGCTCCTTATACTTAGCCGCAATATTGATACCGG
ACAAACTCATGTCAGTAAGC

Product: isochorismate synthase, menaquinone-specific

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALNWLASQQVYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQ
RFLRQHPEHADLRIWGLNAFDPSQGNLLLPRLEWRRCGGKATLRLTLFSERSLQHDAIKAKEFIATLVSIKPLPGLHLTT
TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPE
RLWWRRDKALRTEALAGTVANHPDDKQAQRLGEWLMADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLR
RCIWTALNKADDVICLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKISGNVVRLYA
GAGIVRGSDPEQEWQEIDNKAAGLRTLLQME

Sequences:

>Translated_431_residues
MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALNWLASQQVYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQ
RFLRQHPEHADLRIWGLNAFDPSQGNLLLPRLEWRRCGGKATLRLTLFSERSLQHDAIKAKEFIATLVSIKPLPGLHLTT
TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPE
RLWWRRDKALRTEALAGTVANHPDDKQAQRLGEWLMADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLR
RCIWTALNKADDVICLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKISGNVVRLYA
GAGIVRGSDPEQEWQEIDNKAAGLRTLLQME
>Mature_431_residues
MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALNWLASQQVYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQ
RFLRQHPEHADLRIWGLNAFDPSQGNLLLPRLEWRRCGGKATLRLTLFSERSLQHDAIKAKEFIATLVSIKPLPGLHLTT
TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPE
RLWWRRDKALRTEALAGTVANHPDDKQAQRLGEWLMADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLR
RCIWTALNKADDVICLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKISGNVVRLYA
GAGIVRGSDPEQEWQEIDNKAAGLRTLLQME

Specific function: Menaquinone biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI87082077, Length=431, Percent_Identity=97.9118329466357, Blast_Score=853, Evalue=0.0,
Organism=Escherichia coli, GI1786809, Length=276, Percent_Identity=31.1594202898551, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1787518, Length=291, Percent_Identity=25.7731958762887, Blast_Score=72, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320935, Length=328, Percent_Identity=27.7439024390244, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 48896; Mature: 48896

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALNWLASQQVYPQFYWQQRNG
CCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHCCCC
DEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDPSQGNLLLPRLEWRRCGGK
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEECCCHHHHHCCCC
ATLRLTLFSERSLQHDAIKAKEFIATLVSIKPLPGLHLTTTREQHWPDKTGWTQLIELAT
EEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHCCCCCCHHHHHHHHHH
KTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPE
HHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCHH
RLWWRRDKALRTEALAGTVANHPDDKQAQRLGEWLMADDKNQRENMLVVEDICQRLQADT
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCC
QTLDVLPPQVLRLRKVQHLRRCIWTALNKADDVICLHQLQPTAAVAGLPRDLARQFIARH
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHC
EPFTREWYAGSAGYLSLQQSEFCVSLRSAKISGNVVRLYAGAGIVRGSDPEQEWQEIDNK
CCCCHHHCCCCCCEEEECHHHHHHHHHHCCCCCCEEEEEECCCEECCCCCHHHHHHHHHH
AAGLRTLLQME
HHHHHHHHHCC
>Mature Secondary Structure
MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALNWLASQQVYPQFYWQQRNG
CCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCHHHHHHCCCC
DEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDPSQGNLLLPRLEWRRCGGK
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEECCCHHHHHCCCC
ATLRLTLFSERSLQHDAIKAKEFIATLVSIKPLPGLHLTTTREQHWPDKTGWTQLIELAT
EEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHCCCCCCHHHHHHHHHH
KTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPE
HHHCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCHH
RLWWRRDKALRTEALAGTVANHPDDKQAQRLGEWLMADDKNQRENMLVVEDICQRLQADT
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCC
QTLDVLPPQVLRLRKVQHLRRCIWTALNKADDVICLHQLQPTAAVAGLPRDLARQFIARH
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHC
EPFTREWYAGSAGYLSLQQSEFCVSLRSAKISGNVVRLYAGAGIVRGSDPEQEWQEIDNK
CCCCHHHCCCCCCEEEECHHHHHHHHHHCCCCCCEEEEEECCCEECCCCCHHHHHHHHHH
AAGLRTLLQME
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8549818; 8764478; 9205837; 9278503; 2666397; 7984428 [H]