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The map label for this gene is priC [H]

Identifier: 170680349

GI number: 170680349

Start: 518598

End: 519125

Strand: Reverse

Name: priC [H]

Synonym: EcSMS35_0510

Alternate gene names: 170680349

Gene position: 519125-518598 (Counterclockwise)

Preceding gene: 170680190

Following gene: 170682816

Centisome position: 10.24

GC content: 59.47

Gene sequence:

>528_bases
GTGAAAACCGCCCTGCTGCTGGAAAAACTGGAAGGACAGCTCGCCACGCTGCGTCAGCGTTGTGCCCCGGTAGCGCAATT
TGCCACGCTAAGTGCCCGTTTTGACAGGCACCTTTTTCAGACCCGTGCGACAACACTACAGGCTTGTCTCGATGAGGCGG
GCGATAATCTGTCTGCGCTTCGTCATGCCGTTGAGCAACAGCAACTGCCGCAAGTGGCCTGGCTGGCGGAACATCTGGCG
GCGCAACTGGAGGCTATAGCGCGTGAAGCCACAGCCTGGTCGCTACGCGAGTGGGACAGTGCGCCACCGAAAATTGCCCG
CTGGCAGCGTAAACGTATTCAGCATCAGGATTTTGAGCGGCGGCTACGTGAGATGGTTGCCGAACGTCGAGCCCGCCTGG
CGCGGGTGACCGATCTCGTGGAACAGCAAACGCTGCATCGTGAAGTGGAAGCCTATGAAGCGCGCCTGGCACGCTGCCGC
CATGCGCTGGATAAAATCGAAAACAGGCTAGCGCGTTTAACCCGCTAG

Upstream 100 bases:

>100_bases
AGCCAATGATGATTAAAATGATTCGTTGCATATTGAGTGTTGTCAGCTTACAGAGTTGCTACTTTAGCATAACAATTATC
ATTTTCATTGAGGTCTTATC

Downstream 100 bases:

>100_bases
CAATGGAGAAAGTATGTCACTGGAAAATGCCCCTGACGATGTCAAACTGGCTGTCGATTTGATTGTACTGCTGGAAGAAA
ATCAGATCCCAGCCCGCACC

Product: primosomal replication protein N''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRATTLQACLDEAGDNLSALRHAVEQQQLPQVAWLAEHLA
AQLEAIAREATAWSLREWDSAPPKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
HALDKIENRLARLTR

Sequences:

>Translated_175_residues
MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRATTLQACLDEAGDNLSALRHAVEQQQLPQVAWLAEHLA
AQLEAIAREATAWSLREWDSAPPKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
HALDKIENRLARLTR
>Mature_175_residues
MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRATTLQACLDEAGDNLSALRHAVEQQQLPQVAWLAEHLA
AQLEAIAREATAWSLREWDSAPPKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
HALDKIENRLARLTR

Specific function: PriA recognizes a specific hairpin sequence on bacteriophage phi X174 ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase [H]

COG id: COG3923

COG function: function code L; Primosomal replication protein N''

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786673, Length=175, Percent_Identity=97.7142857142857, Blast_Score=337, Evalue=3e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010890 [H]

Pfam domain/function: PF07445 priB_priC [H]

EC number: NA

Molecular weight: Translated: 20376; Mature: 20376

Theoretical pI: Translated: 10.61; Mature: 10.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRATTLQACLDEAGDNLSAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAPPKIARWQRKRIQHQDFER
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCRHALDKIENRLARLTR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRATTLQACLDEAGDNLSAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
RHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAPPKIARWQRKRIQHQDFER
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCRHALDKIENRLARLTR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1856227; 9278503 [H]