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The map label for this gene is nuoF

Identifier: 170680196

GI number: 170680196

Start: 2487094

End: 2488431

Strand: Reverse

Name: nuoF

Synonym: EcSMS35_2438

Alternate gene names: 170680196

Gene position: 2488431-2487094 (Counterclockwise)

Preceding gene: 170683908

Following gene: 170680130

Centisome position: 49.1

GC content: 56.43

Gene sequence:

>1338_bases
ATGAAAAACATTATCCGTACTCCCGAAACGCATCCGCTGACCTGGCGTCTGCGCGATGACAAACAGCCAGTGTGGCTGGA
CGAATACCGCAGCAAAAACGGTTACGAAGGGGCACGTAAAGCGCTGACCGGGCTGTCGCCTGACGAGATCGTCAACCAGG
TGAAAGATGCTGGCCTGAAAGGGCGCGGCGGCGCAGGCTTCTCGACGGGCCTGAAGTGGAGCCTGATGCCGAAAGACGAA
TCCATGAACATCCGTTACCTGCTGTGTAACGCTGATGAAATGGAGCCGGGCACCTATAAAGACCGCCTGCTGATGGAGCA
ACTGCCGCACCTGCTGGTGGAAGGTATGCTTATCTCCGCGTTTGCGCTGAAAGCTTACCGTGGCTACATCTTCCTGCGCG
GCGAATATATTGAAGCGGCAGTGAATCTGCGCCGTGCCATTGCCGAAGCCACCGAAGCGGGTCTGCTTGGCAAAAACATT
ATGGGAACAGGTTTCGACTTCGAATTGTTCGTCCATACCGGGGCAGGGCGCTACATCTGCGGTGAAGAAACAGCGTTAAT
CAACTCCCTGGAAGGGCGTCGCGCTAACCCACGCTCGAAGCCTCCATTCCCGGCAACCTCCGGTGCATGGGGCAAACCGA
CCTGTGTCAATAACGTCGAAACCCTGTGTAACGTTCCGGCGATCCTTGCTAACGGCGTGGAGTGGTATCAGAACATCTCG
AAAAGTAAAGATGCTGGCACCAAGCTGATGGGCTTCTCCGGTCGGGTGAAAAATCCGGGTCTGTGGGAACTGCCGTTTGG
CACCACCGCACGCGAGATCCTCGAAGATTACGCCGGTGGTATGCGTGACGGTCTGAAATTCAAAGCCTGGCAGCCAGGCG
GCGCGGGCACCGACTTCCTGACCGAAGCGCACCTTGACCTGCCGATGGAATTCGAAAGTATCGGTAAAGCGGGAAGCCGT
CTGGGTACGGCGCTGGCGATGGCTGTTGACCACGAGATCAACATGGTGTCGCTGGTGCGTAACCTGGAAGAGTTTTTCGC
CCGTGAGTCCTGCGGCTGGTGTACGCCGTGCCGCGACGGTCTGCCGTGGAGCGTGAAAATTCTGCGCGCGCTGGAGCGTG
GCGAAGGTCAGCCGGGCGATATCGAAACACTTGAGCAACTGTGTCGATTCTTAGGTCCGGGTAAAACTTTCTGTGCCCAC
GCACCTGGTGCAGTGGAGCCGTTACAGAGCGCCATCAAATATTTCCGCGAAGAATTTGAAGCGGGAATTAAACAGCCGTT
CAGCAATACCCATTTGATTAATGGGATTCAGCCGAACCTGCTGAAAGAGCGCTGGTAA

Upstream 100 bases:

>100_bases
GCCTGGGGAACTGTGATAAAGGGCCAAACATGATGATCGATGAGGATACTCACGCACATCTGACCCCGGAAGCGATCCCT
GAACTGCTGGAGCGGTATAA

Downstream 100 bases:

>100_bases
CCGAATTTCGATTAACGCTCAGTCTCTGACTGAGAAAACTGGAAGCATGCTAATGGCTACAATTCATGTAGACGGCAAAG
AATACGAGGTTAACGGAGCG

Product: NADH dehydrogenase I subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F; NDH-1 subunit F; NUO6

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTGLSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDE
SMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNNVETLCNVPAILANGVEWYQNIS
KSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILEDYAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSR
LGTALAMAVDHEINMVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLGPGKTFCAH
APGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW

Sequences:

>Translated_445_residues
MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTGLSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDE
SMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNNVETLCNVPAILANGVEWYQNIS
KSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILEDYAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSR
LGTALAMAVDHEINMVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLGPGKTFCAH
APGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW
>Mature_445_residues
MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTGLSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDE
SMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNNVETLCNVPAILANGVEWYQNIS
KSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILEDYAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSR
LGTALAMAVDHEINMVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLGPGKTFCAH
APGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family

Homologues:

Organism=Homo sapiens, GI260656005, Length=376, Percent_Identity=44.1489361702128, Blast_Score=322, Evalue=3e-88,
Organism=Homo sapiens, GI20149568, Length=376, Percent_Identity=44.1489361702128, Blast_Score=322, Evalue=4e-88,
Organism=Escherichia coli, GI1788620, Length=445, Percent_Identity=100, Blast_Score=916, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17531727, Length=375, Percent_Identity=42.4, Blast_Score=304, Evalue=6e-83,
Organism=Drosophila melanogaster, GI19920794, Length=375, Percent_Identity=44.5333333333333, Blast_Score=325, Evalue=4e-89,
Organism=Drosophila melanogaster, GI45550452, Length=378, Percent_Identity=43.9153439153439, Blast_Score=315, Evalue=5e-86,
Organism=Drosophila melanogaster, GI24653819, Length=377, Percent_Identity=34.7480106100796, Blast_Score=240, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24653817, Length=352, Percent_Identity=32.6704545454545, Blast_Score=209, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUOF_ECOLI (P31979)

Other databases:

- EMBL:   X68301
- EMBL:   L25055
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   L19569
- PIR:   B65000
- RefSeq:   AP_002882.1
- RefSeq:   NP_416787.1
- ProteinModelPortal:   P31979
- SMR:   P31979
- DIP:   DIP-10382N
- STRING:   P31979
- EnsemblBacteria:   EBESCT00000004753
- EnsemblBacteria:   EBESCT00000004754
- EnsemblBacteria:   EBESCT00000015685
- GeneID:   946753
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2279
- KEGG:   eco:b2284
- EchoBASE:   EB1723
- EcoGene:   EG11774
- eggNOG:   COG1894
- GeneTree:   EBGT00050000011308
- HOGENOM:   HBG669338
- OMA:   LHTGAGR
- ProtClustDB:   PRK11278
- BioCyc:   EcoCyc:NUOF-MONOMER
- BioCyc:   MetaCyc:NUOF-MONOMER
- Genevestigator:   P31979
- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554
- SMART:   SM00928
- TIGRFAMs:   TIGR01959

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB

EC number: =1.6.99.5

Molecular weight: Translated: 49293; Mature: 49293

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00644 COMPLEX1_51K_1; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTGLSPDEIVNQVKDAGLK
CCCCCCCCCCCCEEEEECCCCCCCCHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCC
GRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLISA
CCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNIMGTGFDFELFVHTGAGRYIC
HHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEE
GEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNNVETLCNVPAILANGVEWYQNIS
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHCCHHHHHHHH
KSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILEDYAGGMRDGLKFKAWQPGGAGTDFL
CCCCCCCEEECCCCCCCCCCEEECCCCCHHHHHHHHHCCCHHCCCEEEEECCCCCCCHHH
TEAHLDLPMEFESIGKAGSRLGTALAMAVDHEINMVSLVRNLEEFFARESCGWCTPCRDG
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
LPWSVKILRALERGEGQPGDIETLEQLCRFLGPGKTFCAHAPGAVEPLQSAIKYFREEFE
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHH
AGIKQPFSNTHLINGIQPNLLKERW
HHHHCCCCCCCEECCCCHHHHHCCC
>Mature Secondary Structure
MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTGLSPDEIVNQVKDAGLK
CCCCCCCCCCCCEEEEECCCCCCCCHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCC
GRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDRLLMEQLPHLLVEGMLISA
CCCCCCCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
FALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNIMGTGFDFELFVHTGAGRYIC
HHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEE
GEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNNVETLCNVPAILANGVEWYQNIS
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHCCHHHHHHHH
KSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILEDYAGGMRDGLKFKAWQPGGAGTDFL
CCCCCCCEEECCCCCCCCCCEEECCCCCHHHHHHHHHCCCHHCCCEEEEECCCCCCCHHH
TEAHLDLPMEFESIGKAGSRLGTALAMAVDHEINMVSLVRNLEEFFARESCGWCTPCRDG
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
LPWSVKILRALERGEGQPGDIETLEQLCRFLGPGKTFCAHAPGAVEPLQSAIKYFREEFE
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHH
AGIKQPFSNTHLINGIQPNLLKERW
HHHHCCCCCCCEECCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7690854; 8157582; 9205837; 9278503; 8366049