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The map label for this gene is aroK [H]

Identifier: 170680023

GI number: 170680023

Start: 3725332

End: 3726009

Strand: Reverse

Name: aroK [H]

Synonym: EcSMS35_3666

Alternate gene names: 170680023

Gene position: 3726009-3725332 (Counterclockwise)

Preceding gene: 170681657

Following gene: 170680643

Centisome position: 73.51

GC content: 47.94

Gene sequence:

>678_bases
ATGGGTGATTTATTCAGTTGCCAAACCCGCTGGAGTATTGAGATAATTTTCAGTCTGACTCTCGCAATATCTTATGAGGT
TTCAGTTCATGTCCTGCGGCGCTCTCTGAGCGAAGCGGGTTTATCATTAACGAATAGTCTTAGTAGTACCGAAAAAATGG
CAGAGAAACGCAATATCTTTCTGGTTGGGCCTATGGGTGCCGGAAAAAGCACTATTGGGCGCCAGTTAGCTCAACAACTC
AATATGGAATTTTACGATTCCGATCAAGAGATTGAGAAACGAACCGGAGCTGATGTGGGCTGGGTTTTCGATTTAGAAGG
CGAAGAAGGCTTCCGCGATCGCGAAGAAAAGGTCATCAATGAGTTGACCGAGAAACAGGGTATTGTGCTGGCTACTGGCG
GCGGCTCTGTGAAATCCCGCGAAACGCGTAACCGTCTTTCCGCTCGTGGCGTTGTCGTTTATCTTGAAACGACCATCGAA
AAGCAACTTGCACGCACGCAGCGTGATAAAAAACGCCCGTTGCTGCACGTTGAAACACCGCCGCGTGAAGTTCTGGAAGC
GTTGGCCAATGAACGCAATCCGCTGTATGAAGAGATTGCCGACGTGACCATTCGTACTGATGATCAAAGCGCTAAAGTGG
TCGCAAACCAGATTATTCACATGCTGGAAAGCAACTAA

Upstream 100 bases:

>100_bases
GGCATTTTGCTGTCTTTTTTACGCTAATCTTACCCGGTGATTTATCGCCAGAGCGGTGGTAGCAAGGCAGCGCGCTTGCA
GCGACCAGATATGCAGAGGG

Downstream 100 bases:

>100_bases
TTCTGGCTTTATATACACTCGTCTGCGGGTACAGTAATTAAGGTGGATGTCGCGTTATGGAGAGGATTGTCGTTACTCTC
GGGGAACGTAGTTACCCAAT

Product: shikimate kinase I

Products: NA

Alternate protein names: SK 1 [H]

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MGDLFSCQTRWSIEIIFSLTLAISYEVSVHVLRRSLSEAGLSLTNSLSSTEKMAEKRNIFLVGPMGAGKSTIGRQLAQQL
NMEFYDSDQEIEKRTGADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVYLETTIE
KQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAKVVANQIIHMLESN

Sequences:

>Translated_225_residues
MGDLFSCQTRWSIEIIFSLTLAISYEVSVHVLRRSLSEAGLSLTNSLSSTEKMAEKRNIFLVGPMGAGKSTIGRQLAQQL
NMEFYDSDQEIEKRTGADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVYLETTIE
KQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAKVVANQIIHMLESN
>Mature_224_residues
GDLFSCQTRWSIEIIFSLTLAISYEVSVHVLRRSLSEAGLSLTNSLSSTEKMAEKRNIFLVGPMGAGKSTIGRQLAQQLN
MEFYDSDQEIEKRTGADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVYLETTIEK
QLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAKVVANQIIHMLESN

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate [H]

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family [H]

Homologues:

Organism=Escherichia coli, GI87082255, Length=173, Percent_Identity=100, Blast_Score=354, Evalue=2e-99,
Organism=Escherichia coli, GI1786587, Length=160, Percent_Identity=33.75, Blast_Score=88, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6320332, Length=186, Percent_Identity=31.1827956989247, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000623
- InterPro:   IPR023000 [H]

Pfam domain/function: PF01202 SKI [H]

EC number: =2.7.1.71 [H]

Molecular weight: Translated: 25298; Mature: 25167

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDLFSCQTRWSIEIIFSLTLAISYEVSVHVLRRSLSEAGLSLTNSLSSTEKMAEKRNIF
CCCCCCCCCCCHHEEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCEE
LVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTGADVGWVFDLEGEEGFRDREEKVIN
EEECCCCCHHHHHHHHHHHCCCHHCCCHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHH
ELTEKQGIVLATGGGSVKSRETRNRLSARGVVVYLETTIEKQLARTQRDKKRPLLHVETP
HHHHHCCEEEEECCCCCCHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHCCEEEECCC
PREVLEALANERNPLYEEIADVTIRTDDQSAKVVANQIIHMLESN
HHHHHHHHHCCCCHHHHHHHCCEEEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GDLFSCQTRWSIEIIFSLTLAISYEVSVHVLRRSLSEAGLSLTNSLSSTEKMAEKRNIF
CCCCCCCCCCHHEEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCEE
LVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTGADVGWVFDLEGEEGFRDREEKVIN
EEECCCCCHHHHHHHHHHHCCCHHCCCHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHH
ELTEKQGIVLATGGGSVKSRETRNRLSARGVVVYLETTIEKQLARTQRDKKRPLLHVETP
HHHHHCCEEEEECCCCCCHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHCCEEEECCC
PREVLEALANERNPLYEEIADVTIRTDDQSAKVVANQIIHMLESN
HHHHHHHHHCCCCHHHHHHHCCEEEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA