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The map label for this gene is yqfE [H]

Identifier: 170680003

GI number: 170680003

Start: 3137159

End: 3138055

Strand: Reverse

Name: yqfE [H]

Synonym: EcSMS35_3048

Alternate gene names: 170680003

Gene position: 3138055-3137159 (Counterclockwise)

Preceding gene: 170681536

Following gene: 170684222

Centisome position: 61.91

GC content: 57.53

Gene sequence:

>897_bases
ATGATCGACTGGAATGATTACTACTACTTTGCCCTGATTGCCGATGAAGGCAGCTACAGCCAGGCGGCGCTGCGTGCGGG
TGTGTCGAAATCGGTATTAAGCCGTCGAATGGCAGCGCTGGAAGAGCGCCTCGGCGTGAGGCTTATTCAGCGAACTACGC
GGCGTCTGGCCCTGACGGCGGCAGGGGAACAGTTTGCTGTCGAGTGTCGGGCGCTGGTGGCTCAGGGGGAACGGGCCAGA
GCGGTGGTGATGGCCGCCCAGGAAAGCCCACAGGGAATGCTGCGCGTCAGCGCGCCGGTATTTATGGCTGAAACATGGTT
TGGTGAGTTCGTCAGTGAATTTATCGCCCGCTGGCCCTTGGCGAAGGTGCAACTGCTGGCAATTAATCGCCCGGTGGATA
TGGTCGCTGAAGGCATTGATATTGCGCTGGTTACGGCTTCCGGCGCGCTTGCGGACTCGACGCTGCATTATAAAAAATTA
CATACCCAGGCTGATATTTTAGTTGCCAGTTCGCAGTGGCTGGCCCGCCATCCGCAGGTGAAGGCCATTGACGATCTTAA
CCGCGTCGACGGGATCCTCCGCCTGACGAACAATGGCGGGAGCCAGTGGCAGTTAATGAATGGCGACCAGGAGATTACGC
TGCATCCCGAGGCGCGTTTGCAGACAAATAACATTCGCGTCATGTTGCAGGCAGTGCTTGGCGGGAATGGCGTGGCGTTA
TTGCCTCAATTTGCCTGTAAACAGGGGCTTGCCAACGGTGAACTGGTGCGCCTGTTCGCACCGTGGAGCGGCATACCCAG
ACCGTTGTATGCTTTATTTGCCGGGCGAAAGGGGATGCCCGCCATTGCGCGATATTTTATGGATGAGTTAACCACGCGGC
TTGCCAACGGGGTCTGA

Upstream 100 bases:

>100_bases
GGGGTTGCTCTCATTTCTGCGATGAGAGAAGTTTAATTGTTGTTGATATGGTGATTAAATAGACATAATTGAACTTCATT
GTTGTGAAAATAGAACAATC

Downstream 100 bases:

>100_bases
ATCGCTTTTTTTGTATATAATGCGTGTGAAATTTCATACCACAGGCGAAACGATCATGACGCAGGATGAATTGAAAAAAG
CAGTAGGATGGGCGGCACTT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MIDWNDYYYFALIADEGSYSQAALRAGVSKSVLSRRMAALEERLGVRLIQRTTRRLALTAAGEQFAVECRALVAQGERAR
AVVMAAQESPQGMLRVSAPVFMAETWFGEFVSEFIARWPLAKVQLLAINRPVDMVAEGIDIALVTASGALADSTLHYKKL
HTQADILVASSQWLARHPQVKAIDDLNRVDGILRLTNNGGSQWQLMNGDQEITLHPEARLQTNNIRVMLQAVLGGNGVAL
LPQFACKQGLANGELVRLFAPWSGIPRPLYALFAGRKGMPAIARYFMDELTTRLANGV

Sequences:

>Translated_298_residues
MIDWNDYYYFALIADEGSYSQAALRAGVSKSVLSRRMAALEERLGVRLIQRTTRRLALTAAGEQFAVECRALVAQGERAR
AVVMAAQESPQGMLRVSAPVFMAETWFGEFVSEFIARWPLAKVQLLAINRPVDMVAEGIDIALVTASGALADSTLHYKKL
HTQADILVASSQWLARHPQVKAIDDLNRVDGILRLTNNGGSQWQLMNGDQEITLHPEARLQTNNIRVMLQAVLGGNGVAL
LPQFACKQGLANGELVRLFAPWSGIPRPLYALFAGRKGMPAIARYFMDELTTRLANGV
>Mature_298_residues
MIDWNDYYYFALIADEGSYSQAALRAGVSKSVLSRRMAALEERLGVRLIQRTTRRLALTAAGEQFAVECRALVAQGERAR
AVVMAAQESPQGMLRVSAPVFMAETWFGEFVSEFIARWPLAKVQLLAINRPVDMVAEGIDIALVTASGALADSTLHYKKL
HTQADILVASSQWLARHPQVKAIDDLNRVDGILRLTNNGGSQWQLMNGDQEITLHPEARLQTNNIRVMLQAVLGGNGVAL
LPQFACKQGLANGELVRLFAPWSGIPRPLYALFAGRKGMPAIARYFMDELTTRLANGV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To K.pneumoniae ltrA, E.coli yjiE, and yhcS [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=285, Percent_Identity=27.3684210526316, Blast_Score=120, Evalue=8e-29,
Organism=Escherichia coli, GI1789639, Length=283, Percent_Identity=26.1484098939929, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI87081978, Length=292, Percent_Identity=26.3698630136986, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1789440, Length=266, Percent_Identity=24.0601503759398, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI145693193, Length=283, Percent_Identity=27.208480565371, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787589, Length=263, Percent_Identity=26.9961977186312, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787128, Length=294, Percent_Identity=24.1496598639456, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI157672245, Length=241, Percent_Identity=29.4605809128631, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787879, Length=186, Percent_Identity=29.5698924731183, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1789173, Length=253, Percent_Identity=25.6916996047431, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786713, Length=286, Percent_Identity=26.9230769230769, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788706, Length=256, Percent_Identity=22.65625, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788748, Length=148, Percent_Identity=30.4054054054054, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005119 [H]

Pfam domain/function: PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32793; Mature: 32793

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDWNDYYYFALIADEGSYSQAALRAGVSKSVLSRRMAALEERLGVRLIQRTTRRLALTA
CCCCCCEEEEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
AGEQFAVECRALVAQGERARAVVMAAQESPQGMLRVSAPVFMAETWFGEFVSEFIARWPL
CCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCC
AKVQLLAINRPVDMVAEGIDIALVTASGALADSTLHYKKLHTQADILVASSQWLARHPQV
HHEEEEEECCCHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCHHEEEEECCHHHHHCCCC
KAIDDLNRVDGILRLTNNGGSQWQLMNGDQEITLHPEARLQTNNIRVMLQAVLGGNGVAL
HHHHHHHHHCCEEEEECCCCCEEEEECCCCEEEECCCCEEECCCCEEEEEEHHCCCCEEE
LPQFACKQGLANGELVRLFAPWSGIPRPLYALFAGRKGMPAIARYFMDELTTRLANGV
CCHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIDWNDYYYFALIADEGSYSQAALRAGVSKSVLSRRMAALEERLGVRLIQRTTRRLALTA
CCCCCCEEEEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
AGEQFAVECRALVAQGERARAVVMAAQESPQGMLRVSAPVFMAETWFGEFVSEFIARWPL
CCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCC
AKVQLLAINRPVDMVAEGIDIALVTASGALADSTLHYKKLHTQADILVASSQWLARHPQV
HHEEEEEECCCHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCHHEEEEECCHHHHHCCCC
KAIDDLNRVDGILRLTNNGGSQWQLMNGDQEITLHPEARLQTNNIRVMLQAVLGGNGVAL
HHHHHHHHHCCEEEEECCCCCEEEEECCCCEEEECCCCEEECCCCEEEEEEHHCCCCEEE
LPQFACKQGLANGELVRLFAPWSGIPRPLYALFAGRKGMPAIARYFMDELTTRLANGV
CCHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]