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The map label for this gene is hisF [H]

Identifier: 170679723

GI number: 170679723

Start: 1056480

End: 1057256

Strand: Reverse

Name: hisF [H]

Synonym: EcSMS35_1035

Alternate gene names: 170679723

Gene position: 1057256-1056480 (Counterclockwise)

Preceding gene: 170680218

Following gene: 170679746

Centisome position: 20.86

GC content: 52.38

Gene sequence:

>777_bases
ATGCTGGCAAAACGCATAATTCCGTGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGA
AATCATTGGCGATATCGTGCCGCTAGCAAAACGCTACGCCGAAGAAGGTGCGGACGAGCTGGTGTTCTACGATATCACCG
CTTCCAGCGATGGACGGGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCG
GGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCTCCTGCGCT
GGCGGACCCGACGTTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTCGGTATTGATACCTGGTACG
ACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTC
GACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGG
TTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGG
AACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAAT
ATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAA

Upstream 100 bases:

>100_bases
ACATTAATGATGTCGCTGCCCTGCGTGGCACTGGCGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTC
ACCGTGAAGGAGGCCATCGC

Downstream 100 bases:

>100_bases
CAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGACTTATGCCGGTGATTGTGCAACACGCGGTATCCGGC
GAAGTGCTAATGCTGGGCTA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVA
GGIKSLEDAAKILSFGADKISINSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
IGELKAYLATQGVEIRIC

Sequences:

>Translated_258_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVA
GGIKSLEDAAKILSFGADKISINSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
IGELKAYLATQGVEIRIC
>Mature_258_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVA
GGIKSLEDAAKILSFGADKISINSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
IGELKAYLATQGVEIRIC

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=100, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI87082028, Length=231, Percent_Identity=25.974025974026, Blast_Score=66, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319725, Length=318, Percent_Identity=31.4465408805031, Blast_Score=133, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 28455; Mature: 28455

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISINSPALADPTLITRLADRF
ECHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETLDWVQEVQKRGAGEIVLNMMN
CCEEEEEEECCCCCCCCCCEEEEEECCCCHHHEHHHHHHHHHHHHHHHCCCCCEEHHHHC
QDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IGELKAYLATQGVEIRIC
HHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISINSPALADPTLITRLADRF
ECHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETLDWVQEVQKRGAGEIVLNMMN
CCEEEEEEECCCCCCCCCCEEEEEECCCCHHHEHHHHHHHHHHHHHHHCCCCCEEHHHHC
QDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IGELKAYLATQGVEIRIC
HHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA