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The map label for this gene is 170679704

Identifier: 170679704

GI number: 170679704

Start: 756699

End: 757940

Strand: Reverse

Name: 170679704

Synonym: EcSMS35_0749

Alternate gene names: NA

Gene position: 757940-756699 (Counterclockwise)

Preceding gene: 170683418

Following gene: 170680011

Centisome position: 14.95

GC content: 53.54

Gene sequence:

>1242_bases
ATGAAAATAAAACAGGCTCTGTTCACCGCTGGCTACTCCTCATTCTATTTCGATGACCAGCAGGCGATAAAAAACGGAGC
GGGTCATGACGGCTTTTTTTATACCGGGGAGCCGGTAACACAGGGGTTTAACGCCGTTCGTCAGGCCGGGGAGTGCGTAT
CGGTACAGTTGATTCTGGAAAACGGCGCGGTCGCCGTAGGTGACTGTGCTGCCGTACAGTATTCCGGGGCAGGTGGTCGC
GATCCACTGTTCCTCGCTGAGCACTTTATTCCGTTCCTCAACGACCATATCAAGCCATTACTGGTAGGCCGCGATGTGGA
TGCTTTCCTGCCGAATGCCCGTTTCTTCGACAAATTGCGTATTGACGGCAACTTGCTGCATACCGCCGTGCGCTACGGAT
TATCACAGGCGCTGCTTGATGCTACCGCGCTGGCAACCGGCCGTCTGAAAACTGAAGTGGTCTGTGATGAATGGCAGTTG
CCACGCGTGGCGGAATCCATTCCATTATTTGGTCAGAGCGGGGACGATCGATATATCGCCGTCGATAAGATGATCCTTAA
GGGCATCGACGTGCTGCCCCATGCACTGATTAATAACGTCGAAGAGAAACTGGGCTTTAAAGGTGAAAAACTGCGCGAAT
ATGTCCGCTGGCTGTCGGATCGTATTCTGAGCAAGCGCACCAGCGCACGCTACCACCCTACCCTGCACATCGATGTATAC
GGCACTATCGGTCTGATCTTCGATATGGATCCGCTTCGCTGTGCGCAATACATCGCCAGCCTGGAAAAAGAAGCGCAAGG
CCTGCCGCTCTACATTGAAGGGCCGGTCGATGCCGGTAACAAGCCCGATCAAATTCGCCTGCTGACCGCGATTACCAAAG
AGCTGACGCGCCTCGGTTCCGGCGTGAAAATTGTGGCCGATGAATGGTGTAACACCTACCAGGATATTGTTGATTTCACT
GATGCCGCCAGTTGCCACATGGTGCAAATCAAACCCCCGGATCTGGGCAGTATTCACAACATCGTCGATGCAGTTCTTTA
TTGCAACAGCCACAGCATGGAAGCGTACCAGGGCGGCACCTGCAATGAAACTGATGTCAGTGCCCGCACCTGTGTCCACG
TCGCCCTTGCCGCTCGCCCCATGCGTATGCTGGTAAAACCAGGGATGGGCTTTGACGAAGGCCTCGATATCGTCTTCAAC
GAAATGAATCGTACTATCGCGCTGTTGCAGGCTAAGGATTAA

Upstream 100 bases:

>100_bases
TTGCGGAACGCGCACGCTTTGAAGGACGCCAACCGACATTCCAGATGGTTGTAGATGACATCAATGCTGTATCCCACAGT
AAATTAATAGGAAGACCATA

Downstream 100 bases:

>100_bases
CAAATGGCTCGTTCTTTTAAAGTCTTATCGCCAACGGCAATCCTCGGGTATGGATTCCCGGAAGAGAGCTTCCGCAAAGC
GATGGCAGAATCGCCCGATC

Product: methylaspartate ammonia-lyase

Products: NA

Alternate protein names: Beta-methylaspartase [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFNAVRQAGECVSVQLILENGAVAVGDCAAVQYSGAGGR
DPLFLAEHFIPFLNDHIKPLLVGRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWLSDRILSKRTSARYHPTLHIDVY
GTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPVDAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFT
DAASCHMVQIKPPDLGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFDEGLDIVFN
EMNRTIALLQAKD

Sequences:

>Translated_413_residues
MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFNAVRQAGECVSVQLILENGAVAVGDCAAVQYSGAGGR
DPLFLAEHFIPFLNDHIKPLLVGRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWLSDRILSKRTSARYHPTLHIDVY
GTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPVDAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFT
DAASCHMVQIKPPDLGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFDEGLDIVFN
EMNRTIALLQAKD
>Mature_413_residues
MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFNAVRQAGECVSVQLILENGAVAVGDCAAVQYSGAGGR
DPLFLAEHFIPFLNDHIKPLLVGRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWLSDRILSKRTSARYHPTLHIDVY
GTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPVDAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFT
DAASCHMVQIKPPDLGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFDEGLDIVFN
EMNRTIALLQAKD

Specific function: Unknown

COG id: COG3799

COG function: function code E; Methylaspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006395
- InterPro:   IPR022662
- InterPro:   IPR022665 [H]

Pfam domain/function: PF07476 MAAL_C; PF05034 MAAL_N [H]

EC number: =4.3.1.2 [H]

Molecular weight: Translated: 45545; Mature: 45545

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFNAVRQAGECVSVQLILE
CCHHHHHHHCCCCCEEECCHHHHHCCCCCCCCEECCCHHHHHHHHHHHCCCEEEEEEEEE
NGAVAVGDCAAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLVGRDVDAFLPNARFFDKLR
CCCEEECCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHCCCCHHHHHEE
IDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQLPRVAESIPLFGQSGDDRYIA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCEEE
VDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWLSDRILSKRTSARYHPTLHIDVY
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
GTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPVDAGNKPDQIRLLTAITKELTRLGS
EEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCC
GVKIVADEWCNTYQDIVDFTDAASCHMVQIKPPDLGSIHNIVDAVLYCNSHSMEAYQGGT
CCEEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHCCCCC
CNETDVSARTCVHVALAARPMRMLVKPGMGFDEGLDIVFNEMNRTIALLQAKD
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEEEEECC
>Mature Secondary Structure
MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFNAVRQAGECVSVQLILE
CCHHHHHHHCCCCCEEECCHHHHHCCCCCCCCEECCCHHHHHHHHHHHCCCEEEEEEEEE
NGAVAVGDCAAVQYSGAGGRDPLFLAEHFIPFLNDHIKPLLVGRDVDAFLPNARFFDKLR
CCCEEECCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHCCCCHHHHHEE
IDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQLPRVAESIPLFGQSGDDRYIA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCEEE
VDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWLSDRILSKRTSARYHPTLHIDVY
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
GTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPVDAGNKPDQIRLLTAITKELTRLGS
EEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHCC
GVKIVADEWCNTYQDIVDFTDAASCHMVQIKPPDLGSIHNIVDAVLYCNSHSMEAYQGGT
CCEEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHCCCCC
CNETDVSARTCVHVALAARPMRMLVKPGMGFDEGLDIVFNEMNRTIALLQAKD
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1420191; 8454064; 8428631; 1397267 [H]