Click here to switch to the map view.

The map label for this gene is tgt [H]

Identifier: 170079371

GI number: 170079371

Start: 2902453

End: 2903610

Strand: Reverse

Name: tgt [H]

Synonym: SYNPCC7002_A2780

Alternate gene names: 170079371

Gene position: 2903610-2902453 (Counterclockwise)

Preceding gene: 170079372

Following gene: 170079370

Centisome position: 96.53

GC content: 49.91

Gene sequence:

>1158_bases
TTGATCTTCGGAACATCATCTGTGAGCCATTTTCATTTTTCGCTGCATAAGGAATGTCGCCATACGAATGCCCGAGTCGG
CGTGTTTCATACCCCCCACGGAATTGTGGAAACACCCCGATTTATGCCTGTGGGTACCCTTGCGAATGTAAAAGGCATCA
CGCCGGATCATTTAGTCCAGGCCCAGGCCCAGATGGTTTTATCAAATACTTACCACCTCCACTTACAACCGGGAGAGGAG
ATTGTCCAGAAAGCCGGAGGACTGCATCGGTTTATGGCTTGGGAGGGGCCGATCCTCACGGATTCCGGGGGATTCCAGGT
TTTTAGTCTCAGTGAAATGCGTAAGATTCGTGAGGAAGGGGTAGTTTTCCGTTCGCCGAAGGATGGACGTTTGATTGATA
TGACCCCGGAGCGGTCAATCCAGATTCAAAATGCCCTGGGTGCTGATGTGATCATGGCCTTTGATGAATGTCCACCAGGA
GATGCGGGCCGGGAGGCAGTGGCGGCGGCAACAGCACGAACCTATCGTTGGCTAGAGCGATGTATTGAAGCCCATGAACG
GGACGATCAAGCGTTGTTTGGGATTGTTCAGGGGGGCATTTTCTTAGATTTACGCTCCCAAGCGGTGACAGATTTAACGA
AATTAGACCTACCGGGCTATGCCATCGGGGGCGTTAGTGTGGGGGAATCGCCGGAAAATATTCGTAAAGTGGTACAGCAT
ACAGCGCCGCTCCTGCCGAAAAACAAGATTCGTTATTTGATGGGGGTGGGCACCTATCGGGAAATGGCCCAGGCGATCGC
CGCGGGGATTGATGTGTTTGATTGTGTGATTCCGACGCGGTTGGGGCGTCATGGTGCGGCCCTGGTGCAAGGCGATCGCT
GGAATTTAAAAAATGCCCAGTTTAAAGAAGATTTTCGCCCCTTGGATGAAACTTGTCCTTGTTATACCTGCCAGAATTTC
ACGCGGGCCTATTTGCACCATTTAATCAAAGCGAAAGAAATGTTAGCCTACATGCTACTTTCTTTACATAACGTTACAGA
ATTGATTCAATTTACCGTGAGAATTCGTGAATCAATCCTTAGCGATCGCTTCGTGGAAGAATTCGGCGAATGGCTAGAAC
CCGAACCAACTCATGCTAGGATTGACTGCAAACCATAA

Upstream 100 bases:

>100_bases
GGATGTCCACATGGATGACGATGAAAATTTCAATTTCTAGGTTCTGTTTCCTGAACTCGGTAGAATAAGCGGTGGCTTTT
GCTGCCGTTTTTATTTTTTA

Downstream 100 bases:

>100_bases
GAAAAATCAGTTCAACAAGGAAATATATGGAAGCGGCATTATTACTTGCGAAATTACCTGAGGCCTACTCAATTTTCGAT
CCCCTCGTGGATGTTTTGCC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MIFGTSSVSHFHFSLHKECRHTNARVGVFHTPHGIVETPRFMPVGTLANVKGITPDHLVQAQAQMVLSNTYHLHLQPGEE
IVQKAGGLHRFMAWEGPILTDSGGFQVFSLSEMRKIREEGVVFRSPKDGRLIDMTPERSIQIQNALGADVIMAFDECPPG
DAGREAVAAATARTYRWLERCIEAHERDDQALFGIVQGGIFLDLRSQAVTDLTKLDLPGYAIGGVSVGESPENIRKVVQH
TAPLLPKNKIRYLMGVGTYREMAQAIAAGIDVFDCVIPTRLGRHGAALVQGDRWNLKNAQFKEDFRPLDETCPCYTCQNF
TRAYLHHLIKAKEMLAYMLLSLHNVTELIQFTVRIRESILSDRFVEEFGEWLEPEPTHARIDCKP

Sequences:

>Translated_385_residues
MIFGTSSVSHFHFSLHKECRHTNARVGVFHTPHGIVETPRFMPVGTLANVKGITPDHLVQAQAQMVLSNTYHLHLQPGEE
IVQKAGGLHRFMAWEGPILTDSGGFQVFSLSEMRKIREEGVVFRSPKDGRLIDMTPERSIQIQNALGADVIMAFDECPPG
DAGREAVAAATARTYRWLERCIEAHERDDQALFGIVQGGIFLDLRSQAVTDLTKLDLPGYAIGGVSVGESPENIRKVVQH
TAPLLPKNKIRYLMGVGTYREMAQAIAAGIDVFDCVIPTRLGRHGAALVQGDRWNLKNAQFKEDFRPLDETCPCYTCQNF
TRAYLHHLIKAKEMLAYMLLSLHNVTELIQFTVRIRESILSDRFVEEFGEWLEPEPTHARIDCKP
>Mature_385_residues
MIFGTSSVSHFHFSLHKECRHTNARVGVFHTPHGIVETPRFMPVGTLANVKGITPDHLVQAQAQMVLSNTYHLHLQPGEE
IVQKAGGLHRFMAWEGPILTDSGGFQVFSLSEMRKIREEGVVFRSPKDGRLIDMTPERSIQIQNALGADVIMAFDECPPG
DAGREAVAAATARTYRWLERCIEAHERDDQALFGIVQGGIFLDLRSQAVTDLTKLDLPGYAIGGVSVGESPENIRKVVQH
TAPLLPKNKIRYLMGVGTYREMAQAIAAGIDVFDCVIPTRLGRHGAALVQGDRWNLKNAQFKEDFRPLDETCPCYTCQNF
TRAYLHHLIKAKEMLAYMLLSLHNVTELIQFTVRIRESILSDRFVEEFGEWLEPEPTHARIDCKP

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=367, Percent_Identity=46.3215258855586, Blast_Score=334, Evalue=9e-92,
Organism=Homo sapiens, GI13375872, Length=285, Percent_Identity=24.2105263157895, Blast_Score=102, Evalue=4e-22,
Organism=Escherichia coli, GI1786607, Length=350, Percent_Identity=44.5714285714286, Blast_Score=320, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17544680, Length=346, Percent_Identity=45.3757225433526, Blast_Score=305, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI25146502, Length=234, Percent_Identity=27.3504273504274, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19920510, Length=385, Percent_Identity=44.9350649350649, Blast_Score=335, Evalue=4e-92,
Organism=Drosophila melanogaster, GI21355311, Length=248, Percent_Identity=26.6129032258064, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 43183; Mature: 43183

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFGTSSVSHFHFSLHKECRHTNARVGVFHTPHGIVETPRFMPVGTLANVKGITPDHLVQ
CEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
AQAQMVLSNTYHLHLQPGEEIVQKAGGLHRFMAWEGPILTDSGGFQVFSLSEMRKIREEG
HHHHHHHCCCEEEEECCHHHHHHHCCCCEEEHCCCCCEEECCCCEEEEEHHHHHHHHHCC
VVFRSPKDGRLIDMTPERSIQIQNALGADVIMAFDECPPGDAGREAVAAATARTYRWLER
CEEECCCCCEEEEECCCCCEEEHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
CIEAHERDDQALFGIVQGGIFLDLRSQAVTDLTKLDLPGYAIGGVSVGESPENIRKVVQH
HHHHHCCCCHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHH
TAPLLPKNKIRYLMGVGTYREMAQAIAAGIDVFDCVIPTRLGRHGAALVQGDRWNLKNAQ
HCCCCCCHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCC
FKEDFRPLDETCPCYTCQNFTRAYLHHLIKAKEMLAYMLLSLHNVTELIQFTVRIRESIL
HHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SDRFVEEFGEWLEPEPTHARIDCKP
HHHHHHHHHHHCCCCCCCCEEECCC
>Mature Secondary Structure
MIFGTSSVSHFHFSLHKECRHTNARVGVFHTPHGIVETPRFMPVGTLANVKGITPDHLVQ
CEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
AQAQMVLSNTYHLHLQPGEEIVQKAGGLHRFMAWEGPILTDSGGFQVFSLSEMRKIREEG
HHHHHHHCCCEEEEECCHHHHHHHCCCCEEEHCCCCCEEECCCCEEEEEHHHHHHHHHCC
VVFRSPKDGRLIDMTPERSIQIQNALGADVIMAFDECPPGDAGREAVAAATARTYRWLER
CEEECCCCCEEEEECCCCCEEEHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
CIEAHERDDQALFGIVQGGIFLDLRSQAVTDLTKLDLPGYAIGGVSVGESPENIRKVVQH
HHHHHCCCCHHHHHHHCCCEEEEEHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHH
TAPLLPKNKIRYLMGVGTYREMAQAIAAGIDVFDCVIPTRLGRHGAALVQGDRWNLKNAQ
HCCCCCCHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCC
FKEDFRPLDETCPCYTCQNFTRAYLHHLIKAKEMLAYMLLSLHNVTELIQFTVRIRESIL
HHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SDRFVEEFGEWLEPEPTHARIDCKP
HHHHHHHHHHHCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA