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The map label for this gene is purB [H]

Identifier: 170079247

GI number: 170079247

Start: 2786141

End: 2787436

Strand: Direct

Name: purB [H]

Synonym: SYNPCC7002_A2653

Alternate gene names: 170079247

Gene position: 2786141-2787436 (Clockwise)

Preceding gene: 170079246

Following gene: 170079248

Centisome position: 92.62

GC content: 54.24

Gene sequence:

>1296_bases
GTGATTGAACGTTATACCCTCCCCGAAATGGGGAATCTCTGGACAAATGAAGCAAAACTCAAGGCCTGGCTGAAAGTCGA
AGTTGCCGTCTGTGAAGCGCAAGCAGAGTTAGGGTATATCCCCACAGAAGCCGTAGAAGAAATCAAAGCCAAAGCCCAGT
TCGACGAAGCGCGGGTTTTAGAAATTGAGAAAGAAGTGCGCCATGATGTGATCGCTTTTCTCACCAACGTCAATGAATAT
GTCGGCGATGCGGGGCGTTATATTCACCTCGGCATGACCAGTTCCGATGTTCTGGATACGGCCCTTGCTCTGCAGATGGT
GGATAGCCTCGATCTGATCCTGACCTGTGTCGAAGAACTGATCCAAGCGATTCGCTACCAAGCCCAACAACACCGCTACA
CCGTCATGGTGGGTCGTTCCCACGGCATCCACGCCGAACCGATGACCTTTGGGTTTAAGCTGGCGGGTTGGCTGGCTGAA
ATGCTCCGGAACCGCGATCGCCTAGTCGCTGTGCGCAAAGAAATTGCCGTGGGACAAATCTCTGGCGCTGTGGGAACCTA
TGCGAACATTGAACCCCGCGTTGAAGCTTTAGCTTGTCAAAAACTCGGCCTGGATCCCGATACTGCTTCGACCCAGGTGA
TTTCCCGCGATCGCCACGCCAACTACATGAACCAACTGGCCGTGTTAGCCGCCAGCATCGAACGCTTTGCCGTGGAAATT
CGCAACCTCCAACGCACCGATGTATTAGAAGTCGAAGAATATTTCTCCAAGGGTCAAAAAGGCTCTTCGGCGATGCCCCA
CAAACGCAACCCGATCCGTTCTGAACGCTTAACCGGGATGGCGCGCTTGGTGCGGGGTAATGCGATGGCCGCCCTCGAAA
ACGTTGCCCTCTGGCATGAGCGGGACATTTCCCACAGTTCCGTCGAGCGGGTCGCCGTGCCCGATAGCTGCATCCTCGTG
CATTTTATGCTGCGGGAAACCATTAGCCTCGTGAAGAATCTTTTGGTGTATCCAGAGAACATGAAACGTAACATGAACGT
CTATGGCGGCGTGATTTTCAGTCAGCGGGTACTGCTGGCCCTCGTCGAGAAAGGCTTGACCCGTGAGGATGCCTACCGCC
TTGTGCAAGGTTGCGCCCACACCGCCTGGAACACTGAAGGCGGGAACTTCCGCGCCAACGTCGAGCAAGATGCGGAAATT
ACCCAACACCTCTCTGGCGAGGAGATTGCAGCCTGCTTTGATCCGCAATTCCAACTGCGTCACCTCGATCAAGTCTACCA
ACGCCTCGGTATCTAA

Upstream 100 bases:

>100_bases
CCCAAAGGCGATCGCTTCCCCCGTAAACCCCCGTAAAAACATTGCCAAAATTTGAACAACCCCTTGTAAAATAGCTAGGT
TGTATTCTGCGGCACATCTC

Downstream 100 bases:

>100_bases
ACCTTTCGTTTAACCGAGGTCAATTTGGCGATCGCCTTTTTTGAACTAAGATCCAAGAGAGGCGATTTTTTATGGATAAA
TATTGATCTTCATGGAAACA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIERYTLPEMGNLWTNEAKLKAWLKVEVAVCEAQAELGYIPTEAVEEIKAKAQFDEARVLEIEKEVRHDVIAFLTNVNEY
VGDAGRYIHLGMTSSDVLDTALALQMVDSLDLILTCVEELIQAIRYQAQQHRYTVMVGRSHGIHAEPMTFGFKLAGWLAE
MLRNRDRLVAVRKEIAVGQISGAVGTYANIEPRVEALACQKLGLDPDTASTQVISRDRHANYMNQLAVLAASIERFAVEI
RNLQRTDVLEVEEYFSKGQKGSSAMPHKRNPIRSERLTGMARLVRGNAMAALENVALWHERDISHSSVERVAVPDSCILV
HFMLRETISLVKNLLVYPENMKRNMNVYGGVIFSQRVLLALVEKGLTREDAYRLVQGCAHTAWNTEGGNFRANVEQDAEI
TQHLSGEEIAACFDPQFQLRHLDQVYQRLGI

Sequences:

>Translated_431_residues
MIERYTLPEMGNLWTNEAKLKAWLKVEVAVCEAQAELGYIPTEAVEEIKAKAQFDEARVLEIEKEVRHDVIAFLTNVNEY
VGDAGRYIHLGMTSSDVLDTALALQMVDSLDLILTCVEELIQAIRYQAQQHRYTVMVGRSHGIHAEPMTFGFKLAGWLAE
MLRNRDRLVAVRKEIAVGQISGAVGTYANIEPRVEALACQKLGLDPDTASTQVISRDRHANYMNQLAVLAASIERFAVEI
RNLQRTDVLEVEEYFSKGQKGSSAMPHKRNPIRSERLTGMARLVRGNAMAALENVALWHERDISHSSVERVAVPDSCILV
HFMLRETISLVKNLLVYPENMKRNMNVYGGVIFSQRVLLALVEKGLTREDAYRLVQGCAHTAWNTEGGNFRANVEQDAEI
TQHLSGEEIAACFDPQFQLRHLDQVYQRLGI
>Mature_431_residues
MIERYTLPEMGNLWTNEAKLKAWLKVEVAVCEAQAELGYIPTEAVEEIKAKAQFDEARVLEIEKEVRHDVIAFLTNVNEY
VGDAGRYIHLGMTSSDVLDTALALQMVDSLDLILTCVEELIQAIRYQAQQHRYTVMVGRSHGIHAEPMTFGFKLAGWLAE
MLRNRDRLVAVRKEIAVGQISGAVGTYANIEPRVEALACQKLGLDPDTASTQVISRDRHANYMNQLAVLAASIERFAVEI
RNLQRTDVLEVEEYFSKGQKGSSAMPHKRNPIRSERLTGMARLVRGNAMAALENVALWHERDISHSSVERVAVPDSCILV
HFMLRETISLVKNLLVYPENMKRNMNVYGGVIFSQRVLLALVEKGLTREDAYRLVQGCAHTAWNTEGGNFRANVEQDAEI
TQHLSGEEIAACFDPQFQLRHLDQVYQRLGI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=406, Percent_Identity=27.8325123152709, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI183227688, Length=383, Percent_Identity=27.9373368146214, Blast_Score=144, Evalue=1e-34,
Organism=Escherichia coli, GI1787376, Length=279, Percent_Identity=31.1827956989247, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790398, Length=290, Percent_Identity=26.2068965517241, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17508577, Length=380, Percent_Identity=28.9473684210526, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32564234, Length=349, Percent_Identity=30.6590257879656, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323391, Length=396, Percent_Identity=27.7777777777778, Blast_Score=152, Evalue=8e-38,
Organism=Drosophila melanogaster, GI24647570, Length=380, Percent_Identity=26.3157894736842, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48547; Mature: 48547

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYTLPEMGNLWTNEAKLKAWLKVEVAVCEAQAELGYIPTEAVEEIKAKAQFDEARVL
CCCCCCCCCCCCCCCCCHHHHHHHEEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHH
EIEKEVRHDVIAFLTNVNEYVGDAGRYIHLGMTSSDVLDTALALQMVDSLDLILTCVEEL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IQAIRYQAQQHRYTVMVGRSHGIHAEPMTFGFKLAGWLAEMLRNRDRLVAVRKEIAVGQI
HHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
SGAVGTYANIEPRVEALACQKLGLDPDTASTQVISRDRHANYMNQLAVLAASIERFAVEI
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQRTDVLEVEEYFSKGQKGSSAMPHKRNPIRSERLTGMARLVRGNAMAALENVALWHE
HHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RDISHSSVERVAVPDSCILVHFMLRETISLVKNLLVYPENMKRNMNVYGGVIFSQRVLLA
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHH
LVEKGLTREDAYRLVQGCAHTAWNTEGGNFRANVEQDAEITQHLSGEEIAACFDPQFQLR
HHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHCCCCHHHHHCCCCHHHH
HLDQVYQRLGI
HHHHHHHHHCC
>Mature Secondary Structure
MIERYTLPEMGNLWTNEAKLKAWLKVEVAVCEAQAELGYIPTEAVEEIKAKAQFDEARVL
CCCCCCCCCCCCCCCCCHHHHHHHEEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHH
EIEKEVRHDVIAFLTNVNEYVGDAGRYIHLGMTSSDVLDTALALQMVDSLDLILTCVEEL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IQAIRYQAQQHRYTVMVGRSHGIHAEPMTFGFKLAGWLAEMLRNRDRLVAVRKEIAVGQI
HHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
SGAVGTYANIEPRVEALACQKLGLDPDTASTQVISRDRHANYMNQLAVLAASIERFAVEI
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQRTDVLEVEEYFSKGQKGSSAMPHKRNPIRSERLTGMARLVRGNAMAALENVALWHE
HHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RDISHSSVERVAVPDSCILVHFMLRETISLVKNLLVYPENMKRNMNVYGGVIFSQRVLLA
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHH
LVEKGLTREDAYRLVQGCAHTAWNTEGGNFRANVEQDAEITQHLSGEEIAACFDPQFQLR
HHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHCCCCHHHHHCCCCHHHH
HLDQVYQRLGI
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]