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The map label for this gene is leuB [H]

Identifier: 170078949

GI number: 170078949

Start: 2434948

End: 2436033

Strand: Reverse

Name: leuB [H]

Synonym: SYNPCC7002_A2354

Alternate gene names: 170078949

Gene position: 2436033-2434948 (Counterclockwise)

Preceding gene: 170078954

Following gene: 170078948

Centisome position: 80.98

GC content: 54.14

Gene sequence:

>1086_bases
ATGACCCAGCAATACCGGATCACCCTCCTCCCTGGCGATGGCATTGGCCCCGAGATTTTGGCCGTGGCAGTGGACGTTTT
AAAAACCATTGGCCAACAATTTGATCTAGAGTTTGTTTTTACCGAAGCTTTGATGGGGGGCGCAGCCATTGATGCCACTG
GGGAACCCCTCCCCCAAGCGACCCTAGATGCTTGTAAAGCCAGTGATGCGGTATTGTTTGCGGCCATTGGCGGCTACAAA
TGGGATAACCTTCCCCGGGCGCAACGGCCAGAAACGGGTCTATTGGGTTTGCGGGCGGGCCTGGGTCTGTTTGCAAATTT
GCGTCCGGCCACGATTTTGCCTCAATTGATTGATGCGTCTACCCTGAAGCGGGACGTGGTCGAAGGGGTAGACATTATGG
TGGTGCGGGAACTTACTGGCGGTATTTATTTCGGGAAGCCCAAAGGCAGCGTCACTTTAGAAACGGGCGAGAAACGGGGC
TTTAACACCATGGTCTATGACGAATCGGAAGTGGATCGCATTGCGAAAGTCGCCTTTGACATTGCCCAAAAACGGGGTAA
ACGTCTCTGTTCTGTGGATAAGGCGAATGTCCTCGATGTGTCCCAACTGTGGCGCGATCGCCTGAATCTAATGGCCGAAA
ATTATCCCGATGTGGAACTGTCCCATCTCTACGTTGATAATGCCGCCATGCAGCTTATCCGCGCTCCCAAACAGTTTGAC
ACCATTGTCACGGGGAATTTATTTGGGGACATTCTTTCCGATGCGGCCGCCATGCTTACGGGGAGTATCGGGATGTTGCC
CTCGGCTAGTTTGGGGGCGGATGGCCCTGGGGTTTACGAGCCCGTCCATGGGTCTGCGCCAGATATTGCTGGCCAGGATA
AGGCGAATCCTCTGGCGATGGTGCTCAGTGCGGCGATGATGTTGCGCTATTCTTTTGACCAACCAGAAGCAGCAGCCAAA
ATCGAACAAGCGGTACAAACGGTACTCGATCAAGGCTTCCGCACCGGCGACATCATGTCCGAGGGCATGACTCTCCTGGG
CTGTAAAGGCATGGGCGAAAAACTCTTGGCGGCCCTCAATGCTTAG

Upstream 100 bases:

>100_bases
CCGGGTCAAATAACGTCTCTAGTTTAACATTTGCCCCTTGGGGCCGCCCAAAACCCGCTATCATAAACAGTTGCAAAAAT
ATCTAGATCAACACCCTCCT

Downstream 100 bases:

>100_bases
GTCTCACCTTGGAGGTTGATGCGGGTAAAAAATTCGGCGGGCTATTTTTTTGAAAACACCTTTTGGCCATGGGGAACCTG
AACTTTTCGAGACGGATTCC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MTQQYRITLLPGDGIGPEILAVAVDVLKTIGQQFDLEFVFTEALMGGAAIDATGEPLPQATLDACKASDAVLFAAIGGYK
WDNLPRAQRPETGLLGLRAGLGLFANLRPATILPQLIDASTLKRDVVEGVDIMVVRELTGGIYFGKPKGSVTLETGEKRG
FNTMVYDESEVDRIAKVAFDIAQKRGKRLCSVDKANVLDVSQLWRDRLNLMAENYPDVELSHLYVDNAAMQLIRAPKQFD
TIVTGNLFGDILSDAAAMLTGSIGMLPSASLGADGPGVYEPVHGSAPDIAGQDKANPLAMVLSAAMMLRYSFDQPEAAAK
IEQAVQTVLDQGFRTGDIMSEGMTLLGCKGMGEKLLAALNA

Sequences:

>Translated_361_residues
MTQQYRITLLPGDGIGPEILAVAVDVLKTIGQQFDLEFVFTEALMGGAAIDATGEPLPQATLDACKASDAVLFAAIGGYK
WDNLPRAQRPETGLLGLRAGLGLFANLRPATILPQLIDASTLKRDVVEGVDIMVVRELTGGIYFGKPKGSVTLETGEKRG
FNTMVYDESEVDRIAKVAFDIAQKRGKRLCSVDKANVLDVSQLWRDRLNLMAENYPDVELSHLYVDNAAMQLIRAPKQFD
TIVTGNLFGDILSDAAAMLTGSIGMLPSASLGADGPGVYEPVHGSAPDIAGQDKANPLAMVLSAAMMLRYSFDQPEAAAK
IEQAVQTVLDQGFRTGDIMSEGMTLLGCKGMGEKLLAALNA
>Mature_360_residues
TQQYRITLLPGDGIGPEILAVAVDVLKTIGQQFDLEFVFTEALMGGAAIDATGEPLPQATLDACKASDAVLFAAIGGYKW
DNLPRAQRPETGLLGLRAGLGLFANLRPATILPQLIDASTLKRDVVEGVDIMVVRELTGGIYFGKPKGSVTLETGEKRGF
NTMVYDESEVDRIAKVAFDIAQKRGKRLCSVDKANVLDVSQLWRDRLNLMAENYPDVELSHLYVDNAAMQLIRAPKQFDT
IVTGNLFGDILSDAAAMLTGSIGMLPSASLGADGPGVYEPVHGSAPDIAGQDKANPLAMVLSAAMMLRYSFDQPEAAAKI
EQAVQTVLDQGFRTGDIMSEGMTLLGCKGMGEKLLAALNA

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=330, Percent_Identity=33.6363636363636, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI28178821, Length=312, Percent_Identity=30.1282051282051, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI28178816, Length=312, Percent_Identity=30.1282051282051, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI4758582, Length=346, Percent_Identity=29.1907514450867, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI28178838, Length=332, Percent_Identity=28.9156626506024, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI28178819, Length=148, Percent_Identity=35.8108108108108, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI87081683, Length=355, Percent_Identity=51.5492957746479, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1788101, Length=356, Percent_Identity=33.7078651685393, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1787381, Length=142, Percent_Identity=35.2112676056338, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=340, Percent_Identity=32.9411764705882, Blast_Score=161, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI25144293, Length=339, Percent_Identity=29.7935103244838, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17550882, Length=343, Percent_Identity=29.7376093294461, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17505779, Length=340, Percent_Identity=27.6470588235294, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=349, Percent_Identity=45.5587392550143, Blast_Score=272, Evalue=6e-74,
Organism=Saccharomyces cerevisiae, GI6322097, Length=350, Percent_Identity=34.5714285714286, Blast_Score=166, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6324709, Length=356, Percent_Identity=31.4606741573034, Blast_Score=135, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6324291, Length=349, Percent_Identity=30.945558739255, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24643268, Length=336, Percent_Identity=31.8452380952381, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24643270, Length=341, Percent_Identity=31.9648093841642, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24661184, Length=343, Percent_Identity=32.0699708454811, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI161078635, Length=339, Percent_Identity=27.1386430678466, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI161078633, Length=339, Percent_Identity=27.1386430678466, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24650122, Length=339, Percent_Identity=27.1386430678466, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI161078637, Length=339, Percent_Identity=27.1386430678466, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI161078639, Length=334, Percent_Identity=27.5449101796407, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI281362242, Length=345, Percent_Identity=29.8550724637681, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648872, Length=345, Percent_Identity=29.8550724637681, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI20130355, Length=170, Percent_Identity=28.8235294117647, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38538; Mature: 38407

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQYRITLLPGDGIGPEILAVAVDVLKTIGQQFDLEFVFTEALMGGAAIDATGEPLPQA
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEECCCCCCCCHH
TLDACKASDAVLFAAIGGYKWDNLPRAQRPETGLLGLRAGLGLFANLRPATILPQLIDAS
HHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
TLKRDVVEGVDIMVVRELTGGIYFGKPKGSVTLETGEKRGFNTMVYDESEVDRIAKVAFD
HHHHHHHCCCCEEEEEEHHCCEEEECCCCCEEEECCCCCCCCEEEECHHHHHHHHHHHHH
IAQKRGKRLCSVDKANVLDVSQLWRDRLNLMAENYPDVELSHLYVDNAAMQLIRAPKQFD
HHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCHHHHH
TIVTGNLFGDILSDAAAMLTGSIGMLPSASLGADGPGVYEPVHGSAPDIAGQDKANPLAM
HEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
VLSAAMMLRYSFDQPEAAAKIEQAVQTVLDQGFRTGDIMSEGMTLLGCKGMGEKLLAALN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEEECCCCHHHHHHHHC
A
C
>Mature Secondary Structure 
TQQYRITLLPGDGIGPEILAVAVDVLKTIGQQFDLEFVFTEALMGGAAIDATGEPLPQA
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEECCCCCCCCHH
TLDACKASDAVLFAAIGGYKWDNLPRAQRPETGLLGLRAGLGLFANLRPATILPQLIDAS
HHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
TLKRDVVEGVDIMVVRELTGGIYFGKPKGSVTLETGEKRGFNTMVYDESEVDRIAKVAFD
HHHHHHHCCCCEEEEEEHHCCEEEECCCCCEEEECCCCCCCCEEEECHHHHHHHHHHHHH
IAQKRGKRLCSVDKANVLDVSQLWRDRLNLMAENYPDVELSHLYVDNAAMQLIRAPKQFD
HHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCHHHHH
TIVTGNLFGDILSDAAAMLTGSIGMLPSASLGADGPGVYEPVHGSAPDIAGQDKANPLAM
HEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
VLSAAMMLRYSFDQPEAAAKIEQAVQTVLDQGFRTGDIMSEGMTLLGCKGMGEKLLAALN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEEECCCCHHHHHHHHC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA