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The map label for this gene is rph [H]

Identifier: 170078680

GI number: 170078680

Start: 2157488

End: 2158210

Strand: Direct

Name: rph [H]

Synonym: SYNPCC7002_A2077

Alternate gene names: 170078680

Gene position: 2157488-2158210 (Clockwise)

Preceding gene: 170078676

Following gene: 170078682

Centisome position: 71.72

GC content: 52.7

Gene sequence:

>723_bases
ATGGTTTGGCAGCGTCTGGATAATCGGTTCTTCGATCAACTGCGTCCCTTTAAGTTTGAGCTAGATTTCACAAAATTTGC
CCAGGCTTCTGTTTTAACAAGCTGTGGCGATACCAAGGTTTTGTGCACGGTTTCCGTAGAGGAAGGGGTGCCGCCATTTT
TACGGGATAGTGGCCAAGGCTGGCTCACCGCAGAATATCGGATGATGCCCGGCGCAACCCCAGAACGGCAACGGCGCGAG
TTTATGAAATTATCGGGGCGCACCCAGGAAATTCAACGGCTGATCGGCCGCAGTTTACGGGCGGCAATCGATCTCAAGGC
TTTGGGCGAGCGAACAATTACCATCGATGCGGATGTGCTCCAGGCCGATGGGGGAACGCGGACCACGGCAATCACAGGGG
GTTATGTGGGCTTGGCGATCGCCTGTGAGCGTTTAGTCAAAGCAGGCATCCTAGGAAAATCACCGATTATTGCACCGGTG
GCGGCCATTTCTGTGGGTCTCATCGCCGGGCAAGCTTACCTCGATTTGAATTACCTCGAAGATGTGGCGGCGGATGTGGA
TTTCAATGTGGTGATGATCGGCGGCCAAGGGCTAAACCTCATTGAAGTGCAGGGCACCGCCGAGGCAGGCAGTTATAACC
GCGCCCAACTGAATCAAATTCTCGATGTTGCAGAAAAAGGGATTCAAACCATTATGACCCTACAAACAAAAGCCATTCAC
TAA

Upstream 100 bases:

>100_bases
CCTGCAAGCATCATGTTTGTGGGACTTTCTCGTTTTTTCTCCACGCGGCCTTTGGGAAAGGTTCCGGTATGATGGGCTGT
GTTTGTGTTTTGAGTAAGCT

Downstream 100 bases:

>100_bases
TGGATTTTTTAGGAAAAGGCGATCGCCTTTTTCAGACCCTATTGCGAATTTTGGAGTTGTTGCCGGAGGGTTTGAACTGC
TTCTAGCCAGGGAGCCTGTT

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MVWQRLDNRFFDQLRPFKFELDFTKFAQASVLTSCGDTKVLCTVSVEEGVPPFLRDSGQGWLTAEYRMMPGATPERQRRE
FMKLSGRTQEIQRLIGRSLRAAIDLKALGERTITIDADVLQADGGTRTTAITGGYVGLAIACERLVKAGILGKSPIIAPV
AAISVGLIAGQAYLDLNYLEDVAADVDFNVVMIGGQGLNLIEVQGTAEAGSYNRAQLNQILDVAEKGIQTIMTLQTKAIH

Sequences:

>Translated_240_residues
MVWQRLDNRFFDQLRPFKFELDFTKFAQASVLTSCGDTKVLCTVSVEEGVPPFLRDSGQGWLTAEYRMMPGATPERQRRE
FMKLSGRTQEIQRLIGRSLRAAIDLKALGERTITIDADVLQADGGTRTTAITGGYVGLAIACERLVKAGILGKSPIIAPV
AAISVGLIAGQAYLDLNYLEDVAADVDFNVVMIGGQGLNLIEVQGTAEAGSYNRAQLNQILDVAEKGIQTIMTLQTKAIH
>Mature_240_residues
MVWQRLDNRFFDQLRPFKFELDFTKFAQASVLTSCGDTKVLCTVSVEEGVPPFLRDSGQGWLTAEYRMMPGATPERQRRE
FMKLSGRTQEIQRLIGRSLRAAIDLKALGERTITIDADVLQADGGTRTTAITGGYVGLAIACERLVKAGILGKSPIIAPV
AAISVGLIAGQAYLDLNYLEDVAADVDFNVVMIGGQGLNLIEVQGTAEAGSYNRAQLNQILDVAEKGIQTIMTLQTKAIH

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=212, Percent_Identity=55.188679245283, Blast_Score=219, Evalue=9e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 26136; Mature: 26136

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00097 CARBAMOYLTRANSFERASE ; PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVWQRLDNRFFDQLRPFKFELDFTKFAQASVLTSCGDTKVLCTVSVEEGVPPFLRDSGQG
CCHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHCCCCC
WLTAEYRMMPGATPERQRREFMKLSGRTQEIQRLIGRSLRAAIDLKALGERTITIDADVL
EEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHEE
QADGGTRTTAITGGYVGLAIACERLVKAGILGKSPIIAPVAAISVGLIAGQAYLDLNYLE
ECCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHEEEHHHHH
DVAADVDFNVVMIGGQGLNLIEVQGTAEAGSYNRAQLNQILDVAEKGIQTIMTLQTKAIH
HHHCCCCCEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVWQRLDNRFFDQLRPFKFELDFTKFAQASVLTSCGDTKVLCTVSVEEGVPPFLRDSGQG
CCHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHCCCCC
WLTAEYRMMPGATPERQRREFMKLSGRTQEIQRLIGRSLRAAIDLKALGERTITIDADVL
EEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHEE
QADGGTRTTAITGGYVGLAIACERLVKAGILGKSPIIAPVAAISVGLIAGQAYLDLNYLE
ECCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCHHEEEHHHHH
DVAADVDFNVVMIGGQGLNLIEVQGTAEAGSYNRAQLNQILDVAEKGIQTIMTLQTKAIH
HHHCCCCCEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA