Click here to switch to the map view.

The map label for this gene is fixJ [H]

Identifier: 170078637

GI number: 170078637

Start: 2114782

End: 2115441

Strand: Direct

Name: fixJ [H]

Synonym: SYNPCC7002_A2033

Alternate gene names: 170078637

Gene position: 2114782-2115441 (Clockwise)

Preceding gene: 170078633

Following gene: 170078638

Centisome position: 70.3

GC content: 45.61

Gene sequence:

>660_bases
ATGAAACGAATTCTTGTGATCGATGATGATCCTATCTTGCAAAAGATCCTGGAAAATTTACTCACGCAAGCTGGATATCT
CGTCGCGGTCGTTCTGTCTGGAAAGCAGGGACTCGATATTTGGCTGGAATTTCAACCGGATCTAATCGTTTCTGATGTGC
GTATGCCAGAAATGGATGGTTTTGAATTCTGCCGGTATATTCGTTCTCAACCGACAGGACAGTTGATTCCTTTTCTATTT
TTGTCGAGTCAGACTGATGTGGAGGCGCGTGTTGAGGGTCATTTTTCAGGGGCCGACGACTATATCGCCAAACCTTTTGA
AATGAAGGAATTACTCGCAAAGATTGAGCGCCAGCTAGCCAGAATAGAGAACATTCGCGCAGAAATGCAACGGCTCGCTG
CCCAGGCTAACGTTGAGCAGATGAAGCAAGCCCCAGAACCACTCCCGTTAACCCCCGCCGAGATGCGTGTTTTTTGGGAG
GCAGTTCAAGGTTGGACCAATAAGCAAATCAGTGAGCATCTTTATGTCAGTCCGCGCACTGTCCAGACGCACCTTAGTAG
TATTTTGAATAAATTAAACCTTGAAAATCGGTCTCAATTGGTGCGGTTTGCCTATGAAAATGGCTATGAAAAACCAGCAA
ATCATTCTGAGGACAACTAA

Upstream 100 bases:

>100_bases
CCTTGATCTAACCACGTTTTTTGGGTGGAGAAGAGCTTTCCGAAATGTCTGTAGTCTGTAGACTAACCCGTAAATCTTTA
TTTTCAGTTGGTGCAATGAC

Downstream 100 bases:

>100_bases
TGGGACAGGTTTATGAATTGGCGGTATTGCAGCGGGCGATCGCCACTGATCCAGACCGCTGGCGTCCCCTTGTGTTTACC
AATGGCTGTTTTGATCTGCT

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MKRILVIDDDPILQKILENLLTQAGYLVAVVLSGKQGLDIWLEFQPDLIVSDVRMPEMDGFEFCRYIRSQPTGQLIPFLF
LSSQTDVEARVEGHFSGADDYIAKPFEMKELLAKIERQLARIENIRAEMQRLAAQANVEQMKQAPEPLPLTPAEMRVFWE
AVQGWTNKQISEHLYVSPRTVQTHLSSILNKLNLENRSQLVRFAYENGYEKPANHSEDN

Sequences:

>Translated_219_residues
MKRILVIDDDPILQKILENLLTQAGYLVAVVLSGKQGLDIWLEFQPDLIVSDVRMPEMDGFEFCRYIRSQPTGQLIPFLF
LSSQTDVEARVEGHFSGADDYIAKPFEMKELLAKIERQLARIENIRAEMQRLAAQANVEQMKQAPEPLPLTPAEMRVFWE
AVQGWTNKQISEHLYVSPRTVQTHLSSILNKLNLENRSQLVRFAYENGYEKPANHSEDN
>Mature_219_residues
MKRILVIDDDPILQKILENLLTQAGYLVAVVLSGKQGLDIWLEFQPDLIVSDVRMPEMDGFEFCRYIRSQPTGQLIPFLF
LSSQTDVEARVEGHFSGADDYIAKPFEMKELLAKIERQLARIENIRAEMQRLAAQANVEQMKQAPEPLPLTPAEMRVFWE
AVQGWTNKQISEHLYVSPRTVQTHLSSILNKLNLENRSQLVRFAYENGYEKPANHSEDN

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=228, Percent_Identity=31.5789473684211, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786599, Length=221, Percent_Identity=27.6018099547511, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1789402, Length=220, Percent_Identity=30.4545454545455, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1790437, Length=217, Percent_Identity=32.258064516129, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI87082012, Length=159, Percent_Identity=31.4465408805031, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1788550, Length=132, Percent_Identity=30.3030303030303, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1786784, Length=156, Percent_Identity=30.7692307692308, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787229, Length=161, Percent_Identity=31.055900621118, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789809, Length=166, Percent_Identity=34.9397590361446, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790299, Length=117, Percent_Identity=31.6239316239316, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790860, Length=208, Percent_Identity=26.9230769230769, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788521, Length=200, Percent_Identity=29.5, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786911, Length=163, Percent_Identity=26.9938650306748, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1786747, Length=204, Percent_Identity=25.4901960784314, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1787375, Length=120, Percent_Identity=31.6666666666667, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790552, Length=227, Percent_Identity=25.9911894273128, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1790863, Length=122, Percent_Identity=27.8688524590164, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 25172; Mature: 25172

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRILVIDDDPILQKILENLLTQAGYLVAVVLSGKQGLDIWLEFQPDLIVSDVRMPEMDG
CCEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCEEECCCCCCCCCH
FEFCRYIRSQPTGQLIPFLFLSSQTDVEARVEGHFSGADDYIAKPFEMKELLAKIERQLA
HHHHHHHHCCCCCCCHHHHHCCCCCCCHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHH
RIENIRAEMQRLAAQANVEQMKQAPEPLPLTPAEMRVFWEAVQGWTNKQISEHLYVSPRT
HHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCHH
VQTHLSSILNKLNLENRSQLVRFAYENGYEKPANHSEDN
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKRILVIDDDPILQKILENLLTQAGYLVAVVLSGKQGLDIWLEFQPDLIVSDVRMPEMDG
CCEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCEEECCCCCCCCCH
FEFCRYIRSQPTGQLIPFLFLSSQTDVEARVEGHFSGADDYIAKPFEMKELLAKIERQLA
HHHHHHHHCCCCCCCHHHHHCCCCCCCHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHH
RIENIRAEMQRLAAQANVEQMKQAPEPLPLTPAEMRVFWEAVQGWTNKQISEHLYVSPRT
HHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCHH
VQTHLSSILNKLNLENRSQLVRFAYENGYEKPANHSEDN
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]