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The map label for this gene is yafV [C]

Identifier: 170078616

GI number: 170078616

Start: 2090174

End: 2090986

Strand: Reverse

Name: yafV [C]

Synonym: SYNPCC7002_A2012

Alternate gene names: 170078616

Gene position: 2090986-2090174 (Counterclockwise)

Preceding gene: 170078617

Following gene: 170078613

Centisome position: 69.51

GC content: 50.55

Gene sequence:

>813_bases
ATGAAATCCTATCTCGCGGCAGCGATCCAAATGACCAGTAAGCCCGACTTAGACCACAACTTGGGCGAAGCAGAAGACTT
GATTCAGTTGGCTGTCAACCAGGGGGCGAAACTTGTGACGCTGCCGGAAAATTTTTCTTTCCTCGGCGAAGAAGCCGCTA
AGCTTGCTCAGGCCGATGTCATTGCCCAAAAAAGCGAAAAATTTCTCAAGACTATGGCCCAGAGATTTCAAATCACCCTC
CTGGGTGGTGGGTTTCCGGTGCCTGGCGGTGACAATAAAGTCTTTAACACGGCCCTGCTGATTTCCCCTGATGGCCAAGA
ATTAGCCCGCTATCACAAGGTGCATTTGTTTGATGTGAATTTGCCCGACGGAAATACCTACAAAGAATCAAGTACAGTCC
AGGCGGGCGATACACTCCCCCAAATCTTTGCTTCGCCGGAGTTGGGCAAAATTGGTTTATCCGTTTGTTATGATGTGCGC
TTTCCGGAGCTATATCGTTCGTTATCAAAGCAAGGAGCCGATATTCTTTGCATTCCCGCCGCATTTACAGCCTATACCGG
CAAAGATCACTGGCAAACCTTGATTCAAGCCAGAGCCATTGAAAATACCTGTTATGTAATCGCGCCAGCACAAACAGGCA
ACCATTACGGCATGCGTTATTCCCACGGCCACGCGATGATCGTCGATCCTTGGGGAACTGTGCTGAATGATGCGGGTGTT
CACCCAGGGGTGGCGATCGCCGAAATTAATCCCAATCGCCTAGCCCAAGTGCGCCGTCAAATGCCCTCCCTCGAACACCG
GGTTTTCGGTTAG

Upstream 100 bases:

>100_bases
GTGGGGAAAGATTCTGCTATGGTATTAAACCAGTTTAACCATCAAAACTTCTGGTTGACGGTTTTCCCAGATCCCTATTT
CTAAAAACATCTCAGGCCAT

Downstream 100 bases:

>100_bases
GCTGCAACCAAGACAGCGGTGGGCTTTGGTAAAGTTTCTCCTGCCGCTTCGTAGTCTTCAATTAAGCTTTCTAGGCATTC
AACACCATTTTGAACAGCTT

Product: putative nitrilase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MKSYLAAAIQMTSKPDLDHNLGEAEDLIQLAVNQGAKLVTLPENFSFLGEEAAKLAQADVIAQKSEKFLKTMAQRFQITL
LGGGFPVPGGDNKVFNTALLISPDGQELARYHKVHLFDVNLPDGNTYKESSTVQAGDTLPQIFASPELGKIGLSVCYDVR
FPELYRSLSKQGADILCIPAAFTAYTGKDHWQTLIQARAIENTCYVIAPAQTGNHYGMRYSHGHAMIVDPWGTVLNDAGV
HPGVAIAEINPNRLAQVRRQMPSLEHRVFG

Sequences:

>Translated_270_residues
MKSYLAAAIQMTSKPDLDHNLGEAEDLIQLAVNQGAKLVTLPENFSFLGEEAAKLAQADVIAQKSEKFLKTMAQRFQITL
LGGGFPVPGGDNKVFNTALLISPDGQELARYHKVHLFDVNLPDGNTYKESSTVQAGDTLPQIFASPELGKIGLSVCYDVR
FPELYRSLSKQGADILCIPAAFTAYTGKDHWQTLIQARAIENTCYVIAPAQTGNHYGMRYSHGHAMIVDPWGTVLNDAGV
HPGVAIAEINPNRLAQVRRQMPSLEHRVFG
>Mature_270_residues
MKSYLAAAIQMTSKPDLDHNLGEAEDLIQLAVNQGAKLVTLPENFSFLGEEAAKLAQADVIAQKSEKFLKTMAQRFQITL
LGGGFPVPGGDNKVFNTALLISPDGQELARYHKVHLFDVNLPDGNTYKESSTVQAGDTLPQIFASPELGKIGLSVCYDVR
FPELYRSLSKQGADILCIPAAFTAYTGKDHWQTLIQARAIENTCYVIAPAQTGNHYGMRYSHGHAMIVDPWGTVLNDAGV
HPGVAIAEINPNRLAQVRRQMPSLEHRVFG

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632348, Length=268, Percent_Identity=40.6716417910448, Blast_Score=205, Evalue=3e-53,
Organism=Homo sapiens, GI297632350, Length=268, Percent_Identity=40.6716417910448, Blast_Score=205, Evalue=3e-53,
Organism=Homo sapiens, GI5031947, Length=268, Percent_Identity=40.6716417910448, Blast_Score=205, Evalue=3e-53,
Organism=Homo sapiens, GI9910460, Length=267, Percent_Identity=34.0823970037453, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI297632346, Length=193, Percent_Identity=36.7875647668394, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1786412, Length=241, Percent_Identity=29.4605809128631, Blast_Score=69, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17556280, Length=269, Percent_Identity=40.5204460966543, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6322335, Length=298, Percent_Identity=34.8993288590604, Blast_Score=183, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6323383, Length=271, Percent_Identity=33.5793357933579, Blast_Score=162, Evalue=5e-41,
Organism=Drosophila melanogaster, GI17933642, Length=265, Percent_Identity=38.4905660377359, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI21355835, Length=263, Percent_Identity=30.7984790874525, Blast_Score=148, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 29505; Mature: 29505

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSYLAAAIQMTSKPDLDHNLGEAEDLIQLAVNQGAKLVTLPENFSFLGEEAAKLAQADV
CCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHH
IAQKSEKFLKTMAQRFQITLLGGGFPVPGGDNKVFNTALLISPDGQELARYHKVHLFDVN
HHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCEEEEEEEECCCHHHHHHHEEEEEEEEE
LPDGNTYKESSTVQAGDTLPQIFASPELGKIGLSVCYDVRFPELYRSLSKQGADILCIPA
CCCCCCCCCCCCEECCCCCHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEECC
AFTAYTGKDHWQTLIQARAIENTCYVIAPAQTGNHYGMRYSHGHAMIVDPWGTVLNDAGV
HHHCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEECCCEEEECCCHHHHHCCCC
HPGVAIAEINPNRLAQVRRQMPSLEHRVFG
CCCEEEEEECHHHHHHHHHHCCCHHHHCCC
>Mature Secondary Structure
MKSYLAAAIQMTSKPDLDHNLGEAEDLIQLAVNQGAKLVTLPENFSFLGEEAAKLAQADV
CCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHH
IAQKSEKFLKTMAQRFQITLLGGGFPVPGGDNKVFNTALLISPDGQELARYHKVHLFDVN
HHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCEEEEEEEECCCHHHHHHHEEEEEEEEE
LPDGNTYKESSTVQAGDTLPQIFASPELGKIGLSVCYDVRFPELYRSLSKQGADILCIPA
CCCCCCCCCCCCEECCCCCHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCEEEECC
AFTAYTGKDHWQTLIQARAIENTCYVIAPAQTGNHYGMRYSHGHAMIVDPWGTVLNDAGV
HHHCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEECCCEEEECCCHHHHHCCCC
HPGVAIAEINPNRLAQVRRQMPSLEHRVFG
CCCEEEEEECHHHHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]