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The map label for this gene is hisF [H]

Identifier: 170078244

GI number: 170078244

Start: 1713942

End: 1714724

Strand: Reverse

Name: hisF [H]

Synonym: SYNPCC7002_A1636

Alternate gene names: 170078244

Gene position: 1714724-1713942 (Counterclockwise)

Preceding gene: 170078246

Following gene: 170078240

Centisome position: 57.0

GC content: 56.07

Gene sequence:

>783_bases
ATGCTCGCCAAACGAATTTTGCCTTGCCTCGATGTGAATGCTGGACGGGTTGTCAAAGGTGTGAATTTTGTCGATCTCAA
GGATGCTGGCGATCCGGTAGAGTTGGCGAAGGTTTATAACGATGCGGGGGCTGACGAATTGGTTTTCCTCGACATTACCG
CCACCCACGAAGACCGCGACACCATTATCGATGTGGTCTACCAAACCGCAGAACAGGTCTTTATTCCCCTCACCGTCGGC
GGTGGCATCAGTACCACGGCCCAGATTAGAAACCTCCTGCGGGCCGGGGCCGATAAAGTCAGTATCAATTCTTCGGCGGT
GCGGAACCCGGATTTTATCAATGAAGCAGCCGAACGGTTTGGCAATCAGTGCATTGTGGTGGCGATTGACGCCCGACGTC
GGCCAGATCCGGCAAATCCGGGTTGGGATGTTTATGTGCGGGGAGGCCGCGAAAATACCGGCATTGATGCCCTGGGCTGG
GCGAAGGAAATGGCCCAACGGGGCGCTGGGGAACTGCTCGTCACGAGTATGGATGCTGATGGTACCCAAGCGGGTTACGA
CCTCGAACTGACCAAGGCGATCGCCAATGCCGTCGAAATTCCGGTCATTGCCTCCGGGGGAGCGGGCAACACCCAGCATA
TTTACGAAGCCCTGACGACGGGGAAAGCAGAGGCCGCCCTTTTAGCCTCGTTGCTCCACTATGGTCAACTGACCGTCGCC
GAAATCAAGACCTACCTCCAACAGCATCAAGTGCCCGTGCGCCCAACCCAAATTACTCAGTAA

Upstream 100 bases:

>100_bases
TGAAATTTCTGCTAATTTCTTGGGGGCGATCGCAATTGTGGTGTTGCGAGCAAGGGGTAAAATATGACCTTGCCCCCCGA
CCTAGGAGACCCCCTTTAAA

Downstream 100 bases:

>100_bases
GGGGCAGTAATCGTTGGCCGTAAACCAAGGCTTGGTGGCAATAAACGAGTCCTTCGGCTCGGCTGGCGATCGCCTGAGGT
TTGAGCATTAATTTTTCCTG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLAKRILPCLDVNAGRVVKGVNFVDLKDAGDPVELAKVYNDAGADELVFLDITATHEDRDTIIDVVYQTAEQVFIPLTVG
GGISTTAQIRNLLRAGADKVSINSSAVRNPDFINEAAERFGNQCIVVAIDARRRPDPANPGWDVYVRGGRENTGIDALGW
AKEMAQRGAGELLVTSMDADGTQAGYDLELTKAIANAVEIPVIASGGAGNTQHIYEALTTGKAEAALLASLLHYGQLTVA
EIKTYLQQHQVPVRPTQITQ

Sequences:

>Translated_260_residues
MLAKRILPCLDVNAGRVVKGVNFVDLKDAGDPVELAKVYNDAGADELVFLDITATHEDRDTIIDVVYQTAEQVFIPLTVG
GGISTTAQIRNLLRAGADKVSINSSAVRNPDFINEAAERFGNQCIVVAIDARRRPDPANPGWDVYVRGGRENTGIDALGW
AKEMAQRGAGELLVTSMDADGTQAGYDLELTKAIANAVEIPVIASGGAGNTQHIYEALTTGKAEAALLASLLHYGQLTVA
EIKTYLQQHQVPVRPTQITQ
>Mature_260_residues
MLAKRILPCLDVNAGRVVKGVNFVDLKDAGDPVELAKVYNDAGADELVFLDITATHEDRDTIIDVVYQTAEQVFIPLTVG
GGISTTAQIRNLLRAGADKVSINSSAVRNPDFINEAAERFGNQCIVVAIDARRRPDPANPGWDVYVRGGRENTGIDALGW
AKEMAQRGAGELLVTSMDADGTQAGYDLELTKAIANAVEIPVIASGGAGNTQHIYEALTTGKAEAALLASLLHYGQLTVA
EIKTYLQQHQVPVRPTQITQ

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=43.7984496124031, Blast_Score=199, Evalue=1e-52,
Organism=Escherichia coli, GI87082028, Length=240, Percent_Identity=28.75, Blast_Score=82, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6319725, Length=313, Percent_Identity=33.5463258785943, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27822; Mature: 27822

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRILPCLDVNAGRVVKGVNFVDLKDAGDPVELAKVYNDAGADELVFLDITATHEDRD
CCHHHHCCCCCCCCCCEEECCCEEEECCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCHH
TIIDVVYQTAEQVFIPLTVGGGISTTAQIRNLLRAGADKVSINSSAVRNPDFINEAAERF
HHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHH
GNQCIVVAIDARRRPDPANPGWDVYVRGGRENTGIDALGWAKEMAQRGAGELLVTSMDAD
CCEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCC
GTQAGYDLELTKAIANAVEIPVIASGGAGNTQHIYEALTTGKAEAALLASLLHYGQLTVA
CCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCHHH
EIKTYLQQHQVPVRPTQITQ
HHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLAKRILPCLDVNAGRVVKGVNFVDLKDAGDPVELAKVYNDAGADELVFLDITATHEDRD
CCHHHHCCCCCCCCCCEEECCCEEEECCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCHH
TIIDVVYQTAEQVFIPLTVGGGISTTAQIRNLLRAGADKVSINSSAVRNPDFINEAAERF
HHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHH
GNQCIVVAIDARRRPDPANPGWDVYVRGGRENTGIDALGWAKEMAQRGAGELLVTSMDAD
CCEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCC
GTQAGYDLELTKAIANAVEIPVIASGGAGNTQHIYEALTTGKAEAALLASLLHYGQLTVA
CCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCHHH
EIKTYLQQHQVPVRPTQITQ
HHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]