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The map label for this gene is leuC

Identifier: 170077967

GI number: 170077967

Start: 1410391

End: 1411794

Strand: Reverse

Name: leuC

Synonym: SYNPCC7002_A1353

Alternate gene names: 170077967

Gene position: 1411794-1410391 (Counterclockwise)

Preceding gene: 170077968

Following gene: 170077963

Centisome position: 46.93

GC content: 53.06

Gene sequence:

>1404_bases
ATGAGCAAAGGCACTCTTTTTGATAAGGTTTGGGACTTACACACCGTTAAAGTTTTACCCTCTGGCCAAACGCAACTATT
TATCGGACTGCACCTGATCCATGAAGTGACCAGTCCCCAGGCCTTTGCCATGCTCAGAGACCGCAATCTCCAGGTCATGT
ATCCAGAACGCACGGTCGCTACGGTGGATCACATTGTCCCGACGGAAAATCAAGCGCGACCCTTTGCGGATCCCCTCGCC
GAAGCGATGATGCAGGAACTGGAAAAAAATACGGCTACCAACAAAATTCGTTTTTATAATGTCGGCTCCGGGGGCCAGGG
GATTGTCCATGTGATTGCCCCAGAACAGGGATTAACTCAACCGGGCATGACCGTTGCTTGCGGCGATTCTCACACTTCTA
CCCACGGCGCTTTTGGGGCGATCGCCTTTGGGATTGGGACTTCCCAAGTGCGGGATGTGCTCGCCTCCCAAACCCTAGCT
CTGGCGAAGTTAAAAGTCCGTAAAATCGAAGTAAATGGTGATCTCCAGCCCGGAGTTTATGCCAAAGATGTAATCCTACA
CATTATCCGCAAACTGGGCGTTAAAGGGGGCGTCGGCTACGCCTATGAATATGCCGGGACAACCTTTGAAAAAATGTCCA
TGGAAGAGCGGATGACGGTTTGTAATATGTCCATCGAAGGGGGCGCCCGCTGCGGCTATGTCAACCCCGATTCAGTAACC
TATGACTATTTGAAAGACCGTCCTTTTGCACCCAAGGGAGAAGCATGGGAAAAGGCGATCGCCTGGTGGGATAGTCTCCG
CAGTGAAGCCGACGCCGAATATGACGATGTGGTCACCTTTGACGCAGCAGACATTGCCCCCACCGTCACCTGGGGGATCA
CACCGGGTCAGGGCATTGGCGTTGATGAATCAGTGCCGACCCCAGAAATGATGGCCGAAGATGAACAGGCGATCGCCGCT
GAAGCTTACAAATATATGCAGCTTCAACCAGGCCAAGCGATCCAAGGCACTAAAATTGATGTCTGTTTTATCGGTAGTTG
CACCAATGGTCGCTTGAGCGATCTCCAAGAAGCCGCTAAATATGCCAAAGGCCATCACGTTGCTCCGGGAGTAAAAGCCT
TTGTGGTGCCCGGTTCTGAGCAAGTGAAACAACAGGCTGAGGCCGAGGGACTCGATCGGATTTTTACCGAAGCAGGCTTT
GAATGGCGGGAACCCGGCTGCTCCATGTGTCTCGCCATGAACCCCGATAAGCTCGAAGGCAACCAGATCAGCGCCTCTTC
TTCGAATCGCAATTTTAAAGGAAGGCAGGGTTCTGCGACGGGGCGCACCCTACTCATGAGTCCGGCGATGGTCGTTGCTG
CCGCTGTGACGGGTCAAGTCACCGATGTGCGCACCCTCAACTAA

Upstream 100 bases:

>100_bases
TTGCCCCTCTCCCAAACCCCTGCCCAAAGCGAGGAAAAAAAAGTTGTATGATTGAAGCTCTGCACAGAATATTATTGGCG
TACTTGAGCAATTCAGTTCT

Downstream 100 bases:

>100_bases
ATTATTTCTTCCCCCAAGGTTACTCTTTCACCGAAGATAATACCCTCCGCCTTCAGCACCTCTCTCAAGGGCAATCTTTT
AACGCTCTTCCACTAGAGGG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSKGTLFDKVWDLHTVKVLPSGQTQLFIGLHLIHEVTSPQAFAMLRDRNLQVMYPERTVATVDHIVPTENQARPFADPLA
EAMMQELEKNTATNKIRFYNVGSGGQGIVHVIAPEQGLTQPGMTVACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQTLA
LAKLKVRKIEVNGDLQPGVYAKDVILHIIRKLGVKGGVGYAYEYAGTTFEKMSMEERMTVCNMSIEGGARCGYVNPDSVT
YDYLKDRPFAPKGEAWEKAIAWWDSLRSEADAEYDDVVTFDAADIAPTVTWGITPGQGIGVDESVPTPEMMAEDEQAIAA
EAYKYMQLQPGQAIQGTKIDVCFIGSCTNGRLSDLQEAAKYAKGHHVAPGVKAFVVPGSEQVKQQAEAEGLDRIFTEAGF
EWREPGCSMCLAMNPDKLEGNQISASSSNRNFKGRQGSATGRTLLMSPAMVVAAAVTGQVTDVRTLN

Sequences:

>Translated_467_residues
MSKGTLFDKVWDLHTVKVLPSGQTQLFIGLHLIHEVTSPQAFAMLRDRNLQVMYPERTVATVDHIVPTENQARPFADPLA
EAMMQELEKNTATNKIRFYNVGSGGQGIVHVIAPEQGLTQPGMTVACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQTLA
LAKLKVRKIEVNGDLQPGVYAKDVILHIIRKLGVKGGVGYAYEYAGTTFEKMSMEERMTVCNMSIEGGARCGYVNPDSVT
YDYLKDRPFAPKGEAWEKAIAWWDSLRSEADAEYDDVVTFDAADIAPTVTWGITPGQGIGVDESVPTPEMMAEDEQAIAA
EAYKYMQLQPGQAIQGTKIDVCFIGSCTNGRLSDLQEAAKYAKGHHVAPGVKAFVVPGSEQVKQQAEAEGLDRIFTEAGF
EWREPGCSMCLAMNPDKLEGNQISASSSNRNFKGRQGSATGRTLLMSPAMVVAAAVTGQVTDVRTLN
>Mature_466_residues
SKGTLFDKVWDLHTVKVLPSGQTQLFIGLHLIHEVTSPQAFAMLRDRNLQVMYPERTVATVDHIVPTENQARPFADPLAE
AMMQELEKNTATNKIRFYNVGSGGQGIVHVIAPEQGLTQPGMTVACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQTLAL
AKLKVRKIEVNGDLQPGVYAKDVILHIIRKLGVKGGVGYAYEYAGTTFEKMSMEERMTVCNMSIEGGARCGYVNPDSVTY
DYLKDRPFAPKGEAWEKAIAWWDSLRSEADAEYDDVVTFDAADIAPTVTWGITPGQGIGVDESVPTPEMMAEDEQAIAAE
AYKYMQLQPGQAIQGTKIDVCFIGSCTNGRLSDLQEAAKYAKGHHVAPGVKAFVVPGSEQVKQQAEAEGLDRIFTEAGFE
WREPGCSMCLAMNPDKLEGNQISASSSNRNFKGRQGSATGRTLLMSPAMVVAAAVTGQVTDVRTLN

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI41352693, Length=446, Percent_Identity=24.8878923766816, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI8659555, Length=455, Percent_Identity=23.956043956044, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI4501867, Length=394, Percent_Identity=29.1878172588832, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1786259, Length=463, Percent_Identity=52.9157667386609, Blast_Score=475, Evalue=1e-135,
Organism=Escherichia coli, GI1787531, Length=366, Percent_Identity=26.775956284153, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI2367097, Length=362, Percent_Identity=24.0331491712707, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI25149337, Length=373, Percent_Identity=30.5630026809651, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32564738, Length=373, Percent_Identity=30.5630026809651, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI25149342, Length=318, Percent_Identity=29.874213836478, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=459, Percent_Identity=24.6187363834423, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=475, Percent_Identity=49.4736842105263, Blast_Score=452, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6322261, Length=373, Percent_Identity=31.3672922252011, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6323335, Length=373, Percent_Identity=29.7587131367292, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320440, Length=448, Percent_Identity=27.0089285714286, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI281365315, Length=363, Percent_Identity=28.3746556473829, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI17864292, Length=363, Percent_Identity=28.3746556473829, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI161076999, Length=363, Percent_Identity=28.3746556473829, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28571643, Length=372, Percent_Identity=28.494623655914, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24645686, Length=462, Percent_Identity=25.5411255411255, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI17137564, Length=376, Percent_Identity=25, Blast_Score=85, Evalue=9e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_SYNP2 (B1XLQ6)

Other databases:

- EMBL:   CP000951
- RefSeq:   YP_001734605.1
- ProteinModelPortal:   B1XLQ6
- SMR:   B1XLQ6
- GeneID:   6055123
- GenomeReviews:   CP000951_GR
- KEGG:   syp:SYNPCC7002_A1353
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 50532; Mature: 50401

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKGTLFDKVWDLHTVKVLPSGQTQLFIGLHLIHEVTSPQAFAMLRDRNLQVMYPERTVA
CCCCCCHHHHHCEEEEEEECCCCCEEEEHHHHHHHHCCCHHHHHHHCCCEEEECCCCHHH
TVDHIVPTENQARPFADPLAEAMMQELEKNTATNKIRFYNVGSGGQGIVHVIAPEQGLTQ
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCCCCC
PGMTVACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQTLALAKLKVRKIEVNGDLQPGVY
CCCEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCH
AKDVILHIIRKLGVKGGVGYAYEYAGTTFEKMSMEERMTVCNMSIEGGARCGYVNPDSVT
HHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHEEEEEECCCCEECCCCCCCEE
YDYLKDRPFAPKGEAWEKAIAWWDSLRSEADAEYDDVVTFDAADIAPTVTWGITPGQGIG
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEECCCCCCCCC
VDESVPTPEMMAEDEQAIAAEAYKYMQLQPGQAIQGTKIDVCFIGSCTNGRLSDLQEAAK
CCCCCCCHHHHHCCHHHHHHHHHHHHEECCCCCCCCCEEEEEEEECCCCCHHHHHHHHHH
YAKGHHVAPGVKAFVVPGSEQVKQQAEAEGLDRIFTEAGFEWREPGCSMCLAMNPDKLEG
HHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCC
NQISASSSNRNFKGRQGSATGRTLLMSPAMVVAAAVTGQVTDVRTLN
CEEECCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure 
SKGTLFDKVWDLHTVKVLPSGQTQLFIGLHLIHEVTSPQAFAMLRDRNLQVMYPERTVA
CCCCCHHHHHCEEEEEEECCCCCEEEEHHHHHHHHCCCHHHHHHHCCCEEEECCCCHHH
TVDHIVPTENQARPFADPLAEAMMQELEKNTATNKIRFYNVGSGGQGIVHVIAPEQGLTQ
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEECCCCCCC
PGMTVACGDSHTSTHGAFGAIAFGIGTSQVRDVLASQTLALAKLKVRKIEVNGDLQPGVY
CCCEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCH
AKDVILHIIRKLGVKGGVGYAYEYAGTTFEKMSMEERMTVCNMSIEGGARCGYVNPDSVT
HHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHEEEEEECCCCEECCCCCCCEE
YDYLKDRPFAPKGEAWEKAIAWWDSLRSEADAEYDDVVTFDAADIAPTVTWGITPGQGIG
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEECCCCCCCCC
VDESVPTPEMMAEDEQAIAAEAYKYMQLQPGQAIQGTKIDVCFIGSCTNGRLSDLQEAAK
CCCCCCCHHHHHCCHHHHHHHHHHHHEECCCCCCCCCEEEEEEEECCCCCHHHHHHHHHH
YAKGHHVAPGVKAFVVPGSEQVKQQAEAEGLDRIFTEAGFEWREPGCSMCLAMNPDKLEG
HHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCC
NQISASSSNRNFKGRQGSATGRTLLMSPAMVVAAAVTGQVTDVRTLN
CEEECCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA