Definition Synechococcus sp. PCC 7002 chromosome, complete genome.
Accession NC_010475
Length 3,008,047

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The map label for this gene is pnpA

Identifier: 170077686

GI number: 170077686

Start: 1090538

End: 1092682

Strand: Direct

Name: pnpA

Synonym: SYNPCC7002_A1066

Alternate gene names: 170077686

Gene position: 1090538-1092682 (Clockwise)

Preceding gene: 170077655

Following gene: 170077687

Centisome position: 36.25

GC content: 52.21

Gene sequence:

>2145_bases
ATGCAAGAACATATTCAGTCGATTTCTTTCGATGGGCGAGAAATTCGACTAAAAACAGGTTTGTTTGCGCCTCAGGCCGG
CGGGTCAGTTTTAATTGAATCAGGAGATACAGCGGTTTTAGTCAGTGCAACGACAGCCCCTGGCAGACAAGGAATTGACT
TTCTACCTTTGTTGGTAGATTACGAAGAACGGTTATACGCAGCAGGACGAATTCCCGGTGGTTATTTAAGAAGAGAAGGC
CGCCCCCCAGAACGGGCAACGCTCATTTCGCGCCTCATTGACCGACCGATGCGCCCGCTATTTCCCCAGTGGTTGCGGGA
CGATATTCAGATTGTCGCTACAACGCTCTCCCTCGATGAAGAGGTACCGCCGGATGTCCTCGCTGTGACGGGGGCTTCTG
TGGCCACATTGCTCGCGGGTTTACCTTTCTACGGGCCGATGGCTGCCGTCCGGGTTGGTCTGGTGGGGGATGATTTTATC
CTCAATCCCACGTACCGAGAAATGATGAATGGGGATTTAGACCTCGTAGTTGCTGGCACCCCCGACGGTGTGATCATGGT
TGAGGCCGGAGCCAATCAGTTGCCAGAAGCGGACATGATCGAGGCGATTGATTTCGCCTACGAGGCGATCCAAGAGTTGA
TCGAAGCCCAACGCGCGCTGATGAAGGAACTTGGCGTTGAAATGGTCAGCGCTGAGCCACCTGCTAAGGATGAGGAACTC
GAAAAATTCATTGGCGATCGCACCACGGACAAGATCAAAAAAGTCTTGAGTCAATTTGATCTCGATAAAAATGGTCGCGA
TGCCGCCCTCGATGAAATCAAAGCAACAGAAATCATTGAGGCCATCGAAGCTCTGCCGGAAGATGCACCACTGCGGCTGA
AAACCACAGAAGAGCCGAAGCTCATTGATAATACCTTCAAGGCGCTGACGAAAAAGCTGATGCGGAGCCAAATCGTCGAA
GATGGGGTTCGGGTTGATGGTCGTAAATTGGATCAAGTGCGCCCCATTTCCTGCCGCACTGCTGTGTTACCGAGAGCCGT
CCATGGTAGCGGTTTATTTAACCGAGGTCTGACCCAAGTTTTATCCATTGCTACCCTCGGAACGCCTGGTGATGCCCAAG
AGATGGACGATTTACATCCCTCCGACGAAAAGCGCTACTTGCACCATTACAATTTCCCCCCCTACTCCGTGGGTGAAACT
CGCCCAATGCGATCGCCTGGCCGCCGGGAGATTGGCCACGGTGCCTTGGCCGAACGCGCCCTTTTACCCGTATTGCCGCC
GAAGGATGAATTTCCTTATGTCATTCGGGTCGTTTCTGAAGTCTTATCTTCCAACGGATCCACCTCCATGGGTTCTGTTT
GTGGCTCTACCCTCGCATTGATGGATGCTGGGGTGCCCATTGCCAAGCCCGTGAGTGGTGCCGCGATGGGGCTGATCAAA
GAAGGCAAAGAAGTCCGCATCCTCACCGATATCCAGGGGATTGAAGACTTCCTCGGCGACATGGACTTTAAGGTTGCCGG
GACAGCTGACGGAATTACCGCCCTCCAGATGGACATGAAGATCACTGGCCTAGCCGTTGAAGTGGTCGCAGAAGCGATTA
AGCAAGCTTTACCCGCTCGTCTCCATATCCTCGAAAAGATGACCGATGTCATTGCTGAGCCGAAGGAGTTGTCCCCGGCT
GCTCCCCGTCTACTCACCATTAAGATTGATCCTGATTTAATTGGGATGGTCATTGGCCCCGGTGGTAAAACCATTAAGGG
GATTACCGAACAAACCCGTGCCAAGGTGGATATTGCCGATGACGGTACCGTAACGATCGCCTCCTCTGAATCGGAAAATG
CCGAAAAAGCCAAGCGGCTGATCGTAAACATGACCCGCAAGCTCAATGAAGGGGATGTATATTTCGGTAAAGTCACCCGT
ATTATTCAGATCGGTGCCTTTGTGGAAATTATGCCGGGTAAAGAAGGGATGATTCACATTTCGCAACTGGCAGAAGGCCG
CGTGGGCAAAGTCGAAGATGAAGTTGCTGTCGGTGATGAAGTGGTGGTTAAAGTGCGCGAAATTGATAACAAAGGACGCA
TTAATCTCACCCGCCTAGGGATCCATCCTGATGAAGCAGCCGCTGCTCGGGTTGCTGCTCAATAA

Upstream 100 bases:

>100_bases
GGCGATCGCCAATGCTGTCTAGGATCGGGGCCAGCATAAAGTGTTACGATTGCCACAGAACGAAGAAAAGTGATCATAAA
CAAAAGGACAACACGCAATA

Downstream 100 bases:

>100_bases
AAATATAAAACATCACTATTTTTCGGTGTTGTGATTTAATGTAATTTCCCTCAAGTGACTTGGGGGATTTTTTTTGTTTG
GCACCAGAATTTATCGTTGT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 714; Mature: 714

Protein sequence:

>714_residues
MQEHIQSISFDGREIRLKTGLFAPQAGGSVLIESGDTAVLVSATTAPGRQGIDFLPLLVDYEERLYAAGRIPGGYLRREG
RPPERATLISRLIDRPMRPLFPQWLRDDIQIVATTLSLDEEVPPDVLAVTGASVATLLAGLPFYGPMAAVRVGLVGDDFI
LNPTYREMMNGDLDLVVAGTPDGVIMVEAGANQLPEADMIEAIDFAYEAIQELIEAQRALMKELGVEMVSAEPPAKDEEL
EKFIGDRTTDKIKKVLSQFDLDKNGRDAALDEIKATEIIEAIEALPEDAPLRLKTTEEPKLIDNTFKALTKKLMRSQIVE
DGVRVDGRKLDQVRPISCRTAVLPRAVHGSGLFNRGLTQVLSIATLGTPGDAQEMDDLHPSDEKRYLHHYNFPPYSVGET
RPMRSPGRREIGHGALAERALLPVLPPKDEFPYVIRVVSEVLSSNGSTSMGSVCGSTLALMDAGVPIAKPVSGAAMGLIK
EGKEVRILTDIQGIEDFLGDMDFKVAGTADGITALQMDMKITGLAVEVVAEAIKQALPARLHILEKMTDVIAEPKELSPA
APRLLTIKIDPDLIGMVIGPGGKTIKGITEQTRAKVDIADDGTVTIASSESENAEKAKRLIVNMTRKLNEGDVYFGKVTR
IIQIGAFVEIMPGKEGMIHISQLAEGRVGKVEDEVAVGDEVVVKVREIDNKGRINLTRLGIHPDEAAAARVAAQ

Sequences:

>Translated_714_residues
MQEHIQSISFDGREIRLKTGLFAPQAGGSVLIESGDTAVLVSATTAPGRQGIDFLPLLVDYEERLYAAGRIPGGYLRREG
RPPERATLISRLIDRPMRPLFPQWLRDDIQIVATTLSLDEEVPPDVLAVTGASVATLLAGLPFYGPMAAVRVGLVGDDFI
LNPTYREMMNGDLDLVVAGTPDGVIMVEAGANQLPEADMIEAIDFAYEAIQELIEAQRALMKELGVEMVSAEPPAKDEEL
EKFIGDRTTDKIKKVLSQFDLDKNGRDAALDEIKATEIIEAIEALPEDAPLRLKTTEEPKLIDNTFKALTKKLMRSQIVE
DGVRVDGRKLDQVRPISCRTAVLPRAVHGSGLFNRGLTQVLSIATLGTPGDAQEMDDLHPSDEKRYLHHYNFPPYSVGET
RPMRSPGRREIGHGALAERALLPVLPPKDEFPYVIRVVSEVLSSNGSTSMGSVCGSTLALMDAGVPIAKPVSGAAMGLIK
EGKEVRILTDIQGIEDFLGDMDFKVAGTADGITALQMDMKITGLAVEVVAEAIKQALPARLHILEKMTDVIAEPKELSPA
APRLLTIKIDPDLIGMVIGPGGKTIKGITEQTRAKVDIADDGTVTIASSESENAEKAKRLIVNMTRKLNEGDVYFGKVTR
IIQIGAFVEIMPGKEGMIHISQLAEGRVGKVEDEVAVGDEVVVKVREIDNKGRINLTRLGIHPDEAAAARVAAQ
>Mature_714_residues
MQEHIQSISFDGREIRLKTGLFAPQAGGSVLIESGDTAVLVSATTAPGRQGIDFLPLLVDYEERLYAAGRIPGGYLRREG
RPPERATLISRLIDRPMRPLFPQWLRDDIQIVATTLSLDEEVPPDVLAVTGASVATLLAGLPFYGPMAAVRVGLVGDDFI
LNPTYREMMNGDLDLVVAGTPDGVIMVEAGANQLPEADMIEAIDFAYEAIQELIEAQRALMKELGVEMVSAEPPAKDEEL
EKFIGDRTTDKIKKVLSQFDLDKNGRDAALDEIKATEIIEAIEALPEDAPLRLKTTEEPKLIDNTFKALTKKLMRSQIVE
DGVRVDGRKLDQVRPISCRTAVLPRAVHGSGLFNRGLTQVLSIATLGTPGDAQEMDDLHPSDEKRYLHHYNFPPYSVGET
RPMRSPGRREIGHGALAERALLPVLPPKDEFPYVIRVVSEVLSSNGSTSMGSVCGSTLALMDAGVPIAKPVSGAAMGLIK
EGKEVRILTDIQGIEDFLGDMDFKVAGTADGITALQMDMKITGLAVEVVAEAIKQALPARLHILEKMTDVIAEPKELSPA
APRLLTIKIDPDLIGMVIGPGGKTIKGITEQTRAKVDIADDGTVTIASSESENAEKAKRLIVNMTRKLNEGDVYFGKVTR
IIQIGAFVEIMPGKEGMIHISQLAEGRVGKVEDEVAVGDEVVVKVREIDNKGRINLTRLGIHPDEAAAARVAAQ

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=718, Percent_Identity=36.6295264623955, Blast_Score=434, Evalue=1e-121,
Organism=Escherichia coli, GI145693187, Length=695, Percent_Identity=48.4892086330935, Blast_Score=612, Evalue=1e-176,
Organism=Caenorhabditis elegans, GI115534063, Length=679, Percent_Identity=35.0515463917526, Blast_Score=356, Evalue=3e-98,
Organism=Drosophila melanogaster, GI281362905, Length=698, Percent_Identity=37.6790830945559, Blast_Score=444, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24651641, Length=698, Percent_Identity=37.6790830945559, Blast_Score=444, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24651643, Length=698, Percent_Identity=37.6790830945559, Blast_Score=444, Evalue=1e-124,
Organism=Drosophila melanogaster, GI161079377, Length=642, Percent_Identity=37.5389408099688, Blast_Score=411, Evalue=1e-114,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_SYNP2 (B1XJU0)

Other databases:

- EMBL:   CP000951
- RefSeq:   YP_001734324.1
- ProteinModelPortal:   B1XJU0
- GeneID:   6056074
- GenomeReviews:   CP000951_GR
- KEGG:   syp:SYNPCC7002_A1066
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 77303; Mature: 77303

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MQEHIQSISFDGREIRLKTGLFAPQAGGSVLIESGDTAVLVSATTAPGRQGIDFLPLLVD
CCHHHHHCCCCCCEEEEEECCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCCHHHHHHH
YEERLYAAGRIPGGYLRREGRPPERATLISRLIDRPMRPLFPQWLRDDIQIVATTLSLDE
HHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHEEHHHHHCCC
EVPPDVLAVTGASVATLLAGLPFYGPMAAVRVGLVGDDFILNPTYREMMNGDLDLVVAGT
CCCCCCEEECCHHHHHHHHCCCCCCCHHHHEEEECCCCEEECCHHHHHCCCCEEEEEECC
PDGVIMVEAGANQLPEADMIEAIDFAYEAIQELIEAQRALMKELGVEMVSAEPPAKDEEL
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHH
EKFIGDRTTDKIKKVLSQFDLDKNGRDAALDEIKATEIIEAIEALPEDAPLRLKTTEEPK
HHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCH
LIDNTFKALTKKLMRSQIVEDGVRVDGRKLDQVRPISCRTAVLPRAVHGSGLFNRGLTQV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHH
LSIATLGTPGDAQEMDDLHPSDEKRYLHHYNFPPYSVGETRPMRSPGRREIGHGALAERA
HHHHHCCCCCCHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHC
LLPVLPPKDEFPYVIRVVSEVLSSNGSTSMGSVCGSTLALMDAGVPIAKPVSGAAMGLIK
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
EGKEVRILTDIQGIEDFLGDMDFKVAGTADGITALQMDMKITGLAVEVVAEAIKQALPAR
CCCEEEEEEHHHHHHHHHCCCCEEEECCCCCCEEEEECCEEHHHHHHHHHHHHHHHHHHH
LHILEKMTDVIAEPKELSPAAPRLLTIKIDPDLIGMVIGPGGKTIKGITEQTRAKVDIAD
HHHHHHHHHHHCCCHHCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCHHHHCCEEEECC
DGTVTIASSESENAEKAKRLIVNMTRKLNEGDVYFGKVTRIIQIGAFVEIMPGKEGMIHI
CCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCEEEECCCCCCCEEH
SQLAEGRVGKVEDEVAVGDEVVVKVREIDNKGRINLTRLGIHPDEAAAARVAAQ
HHHCCCCCCCCCHHHHCCHHHEEEEEECCCCCCEEEEEECCCCCHHHHHHHCCC
>Mature Secondary Structure
MQEHIQSISFDGREIRLKTGLFAPQAGGSVLIESGDTAVLVSATTAPGRQGIDFLPLLVD
CCHHHHHCCCCCCEEEEEECCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCCHHHHHHH
YEERLYAAGRIPGGYLRREGRPPERATLISRLIDRPMRPLFPQWLRDDIQIVATTLSLDE
HHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHEEHHHHHCCC
EVPPDVLAVTGASVATLLAGLPFYGPMAAVRVGLVGDDFILNPTYREMMNGDLDLVVAGT
CCCCCCEEECCHHHHHHHHCCCCCCCHHHHEEEECCCCEEECCHHHHHCCCCEEEEEECC
PDGVIMVEAGANQLPEADMIEAIDFAYEAIQELIEAQRALMKELGVEMVSAEPPAKDEEL
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHH
EKFIGDRTTDKIKKVLSQFDLDKNGRDAALDEIKATEIIEAIEALPEDAPLRLKTTEEPK
HHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCH
LIDNTFKALTKKLMRSQIVEDGVRVDGRKLDQVRPISCRTAVLPRAVHGSGLFNRGLTQV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHH
LSIATLGTPGDAQEMDDLHPSDEKRYLHHYNFPPYSVGETRPMRSPGRREIGHGALAERA
HHHHHCCCCCCHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHC
LLPVLPPKDEFPYVIRVVSEVLSSNGSTSMGSVCGSTLALMDAGVPIAKPVSGAAMGLIK
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
EGKEVRILTDIQGIEDFLGDMDFKVAGTADGITALQMDMKITGLAVEVVAEAIKQALPAR
CCCEEEEEEHHHHHHHHHCCCCEEEECCCCCCEEEEECCEEHHHHHHHHHHHHHHHHHHH
LHILEKMTDVIAEPKELSPAAPRLLTIKIDPDLIGMVIGPGGKTIKGITEQTRAKVDIAD
HHHHHHHHHHHCCCHHCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCHHHHCCEEEECC
DGTVTIASSESENAEKAKRLIVNMTRKLNEGDVYFGKVTRIIQIGAFVEIMPGKEGMIHI
CCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCEEEECCCCCCCEEH
SQLAEGRVGKVEDEVAVGDEVVVKVREIDNKGRINLTRLGIHPDEAAAARVAAQ
HHHCCCCCCCCCHHHHCCHHHEEEEEECCCCCCEEEEEECCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA