Click here to switch to the map view.

The map label for this gene is truA

Identifier: 170077660

GI number: 170077660

Start: 1075377

End: 1076201

Strand: Reverse

Name: truA

Synonym: SYNPCC7002_A1040

Alternate gene names: 170077660

Gene position: 1076201-1075377 (Counterclockwise)

Preceding gene: 170077661

Following gene: 170077659

Centisome position: 35.78

GC content: 48.24

Gene sequence:

>825_bases
ATGCCCATGTCCCAAGCCAACCCCAGAAAACGCATCGCGTTGGTCATCCAATATATCGGAACTCACTTTTGTGGTTGGCA
ACGCCAAACAGCAGAAAGAACAGTGCAAGCCGTCATTGAAGAGACAATCTCCACCGTTGTCGGCCAAAAAATCACAATTC
ACGGTGCCGGCCGAACCGATAGCGGTGTCCATGCCGCCGGACAATTCGCCCACTTTGAATGTGAAAGCCTAATTCCTGGT
TATAAATGGGCCAAAATCCTCAACGATCGCCTGCCACCCGATATCAATATCCGTGGTGCTTGCGAAGTTTCCCAGGATTG
GCACGCACAATTTTCTGCCCAATGGCGACGGTATCGATACACCATCTATACCCACCCAACCCCAAACCTGTTCATTCAGC
CCTATGCTTGGCATTATTACCAAGCCCCCCTCGATGAGACTCTCATCCAAGCAGCCCTGACGCCCCTATTGGGTGAACAT
CACCTCGCTGCTTTTCAACGGACTGGTTCCAAGCGATCCCATGCATGGGTCAATGTACAGGCAGCAGAATGTTCCCGTCG
AGGCTCCTTTATTCATATAGAAATTCAAGCCAATGGGTTTTTGTATGGGATGGTTCGCCTACTGGTCGGGATGCTAGTGG
ACATTGGACAAGGTAAACTTTCTTTAGCGCAATTCCAAGATATTTGGAAAAATGAGAAGCGACACCTTGTAAAATATTCA
GCCCCAGCAAAAGGCTTATGTTTACTTAGGGTTGGTTATCCTGAGCCTTTGTTTCCACCCCATATTTGGTTTGATACCCA
GCCAACTTTTCTACTAACTCATTAG

Upstream 100 bases:

>100_bases
GGGCTATAGCCGCGTTGTGCGCACCGTTCGTCGTCGCGGTGACAACGCAGAAATGGCAATTATCGAATTAGTCTAACTCA
GACTAATCTGCCAACTGTCC

Downstream 100 bases:

>100_bases
TTGAATTCCTTATGCGTCATCTAGCGCAAAAATTTTATGATTAAGACACCTTTACCGAAACAAGAAACCCTCGACCAGAA
ATGGTACGTGGTCGATGCTG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MPMSQANPRKRIALVIQYIGTHFCGWQRQTAERTVQAVIEETISTVVGQKITIHGAGRTDSGVHAAGQFAHFECESLIPG
YKWAKILNDRLPPDINIRGACEVSQDWHAQFSAQWRRYRYTIYTHPTPNLFIQPYAWHYYQAPLDETLIQAALTPLLGEH
HLAAFQRTGSKRSHAWVNVQAAECSRRGSFIHIEIQANGFLYGMVRLLVGMLVDIGQGKLSLAQFQDIWKNEKRHLVKYS
APAKGLCLLRVGYPEPLFPPHIWFDTQPTFLLTH

Sequences:

>Translated_274_residues
MPMSQANPRKRIALVIQYIGTHFCGWQRQTAERTVQAVIEETISTVVGQKITIHGAGRTDSGVHAAGQFAHFECESLIPG
YKWAKILNDRLPPDINIRGACEVSQDWHAQFSAQWRRYRYTIYTHPTPNLFIQPYAWHYYQAPLDETLIQAALTPLLGEH
HLAAFQRTGSKRSHAWVNVQAAECSRRGSFIHIEIQANGFLYGMVRLLVGMLVDIGQGKLSLAQFQDIWKNEKRHLVKYS
APAKGLCLLRVGYPEPLFPPHIWFDTQPTFLLTH
>Mature_273_residues
PMSQANPRKRIALVIQYIGTHFCGWQRQTAERTVQAVIEETISTVVGQKITIHGAGRTDSGVHAAGQFAHFECESLIPGY
KWAKILNDRLPPDINIRGACEVSQDWHAQFSAQWRRYRYTIYTHPTPNLFIQPYAWHYYQAPLDETLIQAALTPLLGEHH
LAAFQRTGSKRSHAWVNVQAAECSRRGSFIHIEIQANGFLYGMVRLLVGMLVDIGQGKLSLAQFQDIWKNEKRHLVKYSA
PAKGLCLLRVGYPEPLFPPHIWFDTQPTFLLTH

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=277, Percent_Identity=32.129963898917, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1788657, Length=255, Percent_Identity=37.2549019607843, Blast_Score=173, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_SYNP2 (B1XJI2)

Other databases:

- EMBL:   CP000951
- RefSeq:   YP_001734298.1
- ProteinModelPortal:   B1XJI2
- SMR:   B1XJI2
- GeneID:   6057759
- GenomeReviews:   CP000951_GR
- KEGG:   syp:SYNPCC7002_A1040
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 31242; Mature: 31111

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMSQANPRKRIALVIQYIGTHFCGWQRQTAERTVQAVIEETISTVVGQKITIHGAGRTD
CCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCC
SGVHAAGQFAHFECESLIPGYKWAKILNDRLPPDINIRGACEVSQDWHAQFSAQWRRYRY
CCCCCCCCEEEEEHHHCCCCHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHCEEEEEE
TIYTHPTPNLFIQPYAWHYYQAPLDETLIQAALTPLLGEHHLAAFQRTGSKRSHAWVNVQ
EEEECCCCCEEEECCEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEEE
AAECSRRGSFIHIEIQANGFLYGMVRLLVGMLVDIGQGKLSLAQFQDIWKNEKRHLVKYS
HHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEC
APAKGLCLLRVGYPEPLFPPHIWFDTQPTFLLTH
CCCCCEEEEEECCCCCCCCCEEEECCCCCEEEEC
>Mature Secondary Structure 
PMSQANPRKRIALVIQYIGTHFCGWQRQTAERTVQAVIEETISTVVGQKITIHGAGRTD
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCC
SGVHAAGQFAHFECESLIPGYKWAKILNDRLPPDINIRGACEVSQDWHAQFSAQWRRYRY
CCCCCCCCEEEEEHHHCCCCHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHCEEEEEE
TIYTHPTPNLFIQPYAWHYYQAPLDETLIQAALTPLLGEHHLAAFQRTGSKRSHAWVNVQ
EEEECCCCCEEEECCEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEEE
AAECSRRGSFIHIEIQANGFLYGMVRLLVGMLVDIGQGKLSLAQFQDIWKNEKRHLVKYS
HHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEC
APAKGLCLLRVGYPEPLFPPHIWFDTQPTFLLTH
CCCCCEEEEEECCCCCCCCCEEEECCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA