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The map label for this gene is rlmN

Identifier: 170077338

GI number: 170077338

Start: 746668

End: 747708

Strand: Direct

Name: rlmN

Synonym: SYNPCC7002_A0715

Alternate gene names: 170077338

Gene position: 746668-747708 (Clockwise)

Preceding gene: 170077332

Following gene: 170077339

Centisome position: 24.82

GC content: 54.27

Gene sequence:

>1041_bases
ATGCCCCAAGACGTACTCCTCGGCAAAAGTCTCCCGGAACTGACCGACTGGATCGAAACCACTGGTCAACCCGCCTACCG
TGGCAAACAGCTCTACAATTGGCTCTACCAAAAAGGGATCCATGACCTGAGTGAAATCACCGTCTTTCCGAAGGCTTGGC
GTGAGCAAATGGGCACCTATCCCGTGGGGCGATCGCAGATCCACCACCAACGCACCGCCCCCGATGGCACCCGCAAATAT
CTCCTCCAACTCCATGACGGTTTAATTATTGAGACCGTTGGCATCCCCACGGAAAAACGCCTCACTGTCTGTGTTTCTTC
TCAGGTGGGCTGTGCCATGGCCTGTGATTTTTGTGCCACCGGCAAAAGTGGCTTTACCCGGCACCTCCAGGCCCACGAAA
TTATTGATCAAGTGCTCACTGTCCAAACAGACTTCCAGCAACGGGTCAGCCATGTGGTGTTTATGGGCATGGGGGAACCC
CTCGCCAACCTTGAACAGGTGCTCAAATCGATCCAAAGCCTGAACCAAGACATCGGTATCGGCCAGCGATCGCTGACAGT
TTCTACCGTGGGGGTGCCGGATCAGATCCGCGCCCTGGCCCAGCAGAATCTGCAAATTACCCTAGCGGTAAGCCTCCACG
CCCCGAACCAAGCCCTCCGGGAATCGATTATTCCCACCGCTGTCCACTATCCCATTGAGGCTCTGTTGGACGAATGCCGC
GAGTACGTCGCCATCACCCGTCGCCGCCTCAGTTTTGAATACATTTTGCTGGCGGGGGTGAATGATCTGCCCGACCATGC
CGCCGAATTAGCCAAAAAACTAAAGGGGTTCCAAAGCCATGTGAATTTGATTCCCTACAATCCCATTACCGAAGTGCCTT
TCCAGCGACCAGGGAAAAAACGGATCAATGTCTTTAAGCAAATCCTCCAGGATCACAAGATTGCTGTGAGCGTACGTTAT
TCTAAGGGCTTGGAGGCGGATGCGGCCTGTGGTCAACTGCGATCAAACCTCCGACGATCTGCCCCGGCAACGGTAAAATA
A

Upstream 100 bases:

>100_bases
TTACCAGGAACCAACGGCCCCTATGCCACAATGGAAAAGGAATTTTTTAACTTTATCCCTTAACCCAATCACAGGCGATC
GCCCCTTTCCCTTGCAACCC

Downstream 100 bases:

>100_bases
AACAGCAAAAACCCACCCTTAACCCCCAGAATCTATGAATATTTTCGGGATTGGCCTGCCAGAAATGGCCCTCATTTTTA
TTATTGCTCTGTTAATCTTT

Product: radical SAM protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MPQDVLLGKSLPELTDWIETTGQPAYRGKQLYNWLYQKGIHDLSEITVFPKAWREQMGTYPVGRSQIHHQRTAPDGTRKY
LLQLHDGLIIETVGIPTEKRLTVCVSSQVGCAMACDFCATGKSGFTRHLQAHEIIDQVLTVQTDFQQRVSHVVFMGMGEP
LANLEQVLKSIQSLNQDIGIGQRSLTVSTVGVPDQIRALAQQNLQITLAVSLHAPNQALRESIIPTAVHYPIEALLDECR
EYVAITRRRLSFEYILLAGVNDLPDHAAELAKKLKGFQSHVNLIPYNPITEVPFQRPGKKRINVFKQILQDHKIAVSVRY
SKGLEADAACGQLRSNLRRSAPATVK

Sequences:

>Translated_346_residues
MPQDVLLGKSLPELTDWIETTGQPAYRGKQLYNWLYQKGIHDLSEITVFPKAWREQMGTYPVGRSQIHHQRTAPDGTRKY
LLQLHDGLIIETVGIPTEKRLTVCVSSQVGCAMACDFCATGKSGFTRHLQAHEIIDQVLTVQTDFQQRVSHVVFMGMGEP
LANLEQVLKSIQSLNQDIGIGQRSLTVSTVGVPDQIRALAQQNLQITLAVSLHAPNQALRESIIPTAVHYPIEALLDECR
EYVAITRRRLSFEYILLAGVNDLPDHAAELAKKLKGFQSHVNLIPYNPITEVPFQRPGKKRINVFKQILQDHKIAVSVRY
SKGLEADAACGQLRSNLRRSAPATVK
>Mature_345_residues
PQDVLLGKSLPELTDWIETTGQPAYRGKQLYNWLYQKGIHDLSEITVFPKAWREQMGTYPVGRSQIHHQRTAPDGTRKYL
LQLHDGLIIETVGIPTEKRLTVCVSSQVGCAMACDFCATGKSGFTRHLQAHEIIDQVLTVQTDFQQRVSHVVFMGMGEPL
ANLEQVLKSIQSLNQDIGIGQRSLTVSTVGVPDQIRALAQQNLQITLAVSLHAPNQALRESIIPTAVHYPIEALLDECRE
YVAITRRRLSFEYILLAGVNDLPDHAAELAKKLKGFQSHVNLIPYNPITEVPFQRPGKKRINVFKQILQDHKIAVSVRYS
KGLEADAACGQLRSNLRRSAPATVK

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=345, Percent_Identity=35.0724637681159, Blast_Score=220, Evalue=9e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_SYNP2 (B1XQH8)

Other databases:

- EMBL:   CP000951
- RefSeq:   YP_001733976.1
- ProteinModelPortal:   B1XQH8
- SMR:   B1XQH8
- GeneID:   6057154
- GenomeReviews:   CP000951_GR
- KEGG:   syp:SYNPCC7002_A0715
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 38672; Mature: 38541

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQDVLLGKSLPELTDWIETTGQPAYRGKQLYNWLYQKGIHDLSEITVFPKAWREQMGTY
CCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHCCC
PVGRSQIHHQRTAPDGTRKYLLQLHDGLIIETVGIPTEKRLTVCVSSQVGCAMACDFCAT
CCCHHHHHHHCCCCCHHHHHHHHHHCCEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHC
GKSGFTRHLQAHEIIDQVLTVQTDFQQRVSHVVFMGMGEPLANLEQVLKSIQSLNQDIGI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHCCCCCHHHHHHHHHHHHHHHHHHCC
GQRSLTVSTVGVPDQIRALAQQNLQITLAVSLHAPNQALRESIIPTAVHYPIEALLDECR
CCCEEEEEECCCCHHHHHHHHCCCEEEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHH
EYVAITRRRLSFEYILLAGVNDLPDHAAELAKKLKGFQSHVNLIPYNPITEVPFQRPGKK
HHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
RINVFKQILQDHKIAVSVRYSKGLEADAACGQLRSNLRRSAPATVK
HHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PQDVLLGKSLPELTDWIETTGQPAYRGKQLYNWLYQKGIHDLSEITVFPKAWREQMGTY
CCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHCCC
PVGRSQIHHQRTAPDGTRKYLLQLHDGLIIETVGIPTEKRLTVCVSSQVGCAMACDFCAT
CCCHHHHHHHCCCCCHHHHHHHHHHCCEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHC
GKSGFTRHLQAHEIIDQVLTVQTDFQQRVSHVVFMGMGEPLANLEQVLKSIQSLNQDIGI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHCCCCCHHHHHHHHHHHHHHHHHHCC
GQRSLTVSTVGVPDQIRALAQQNLQITLAVSLHAPNQALRESIIPTAVHYPIEALLDECR
CCCEEEEEECCCCHHHHHHHHCCCEEEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHH
EYVAITRRRLSFEYILLAGVNDLPDHAAELAKKLKGFQSHVNLIPYNPITEVPFQRPGKK
HHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHH
RINVFKQILQDHKIAVSVRYSKGLEADAACGQLRSNLRRSAPATVK
HHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA