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The map label for this gene is pcm

Identifier: 170077301

GI number: 170077301

Start: 707723

End: 708391

Strand: Direct

Name: pcm

Synonym: SYNPCC7002_A0678

Alternate gene names: 170077301

Gene position: 707723-708391 (Clockwise)

Preceding gene: 170077300

Following gene: 170077302

Centisome position: 23.53

GC content: 55.31

Gene sequence:

>669_bases
ATGGATCTTCCACCCACCAATGATTTCTGTGCCGATCCCACCGTTGCTTTACGTCAGCGGATGGTTAAGCAGCAAATTAT
CGCCAGGGGCGTGAACGATCCAGCTGTCTTAGCCGCGCTCCAGCAAGTGCCCCGGCATCGCTTTGTGCCTGATTCTCTCC
AAAATCTCGCCTACGCCGATCAACCCCTTACCATCGGCTATGGCCAAACTATTTCCCAGCCCTATATCGTCGCCTACATG
ACCGAGGCGGCCCACCTGACGCCCAGCAGCAAAGTTTTAGAAATTGGTACGGGGTGCGGTTATCAAGCGGCGATCCTCGC
CGAAATTGCCCAGGAAGTTTTTACCGTTGAAGTTGTGCCCGAATTAGCCCGGCAAGCCCGCGATCGCCTCGAAGCATTGG
GCTATCAGAATATTCACTACAAGATTGGTGACGGTTACCAGGGCTGGTCAGAATTTGCTCCCTACGACGCGATCCTTGTT
ACGGCGGCCCCAGACCATCGGCCCCAACCGTTACTGCAACAATTGGCCGTGGGCGGTCACCTGGTAATACCTGTGGGCAC
CGTCGGCCAACGCCTTGAAGTCCTCCACAAAACCAGCACTGACTTGGAAATGGAAAAGGCGATCGCTGTGCGGTTTGTGC
CACTCCAGGGTCACAGCTACGGATTTTGA

Upstream 100 bases:

>100_bases
GACCCAACAACAGCGGCCCGACTTTCTCTAGATTCCAGGAGTACAATGGAGGTAGGAGGAAGTCGAAGGATTCATACTGA
AATGATCCTCGCCAGCGACC

Downstream 100 bases:

>100_bases
GTAAATTTGGATAGGAATTGTCCCATCTCTATTGCTCGCATCGAGACTTCCTGGAGAGTGACCTAGACGGTTTGGGAATC
CGGCGCAACGGCCACATATT

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MDLPPTNDFCADPTVALRQRMVKQQIIARGVNDPAVLAALQQVPRHRFVPDSLQNLAYADQPLTIGYGQTISQPYIVAYM
TEAAHLTPSSKVLEIGTGCGYQAAILAEIAQEVFTVEVVPELARQARDRLEALGYQNIHYKIGDGYQGWSEFAPYDAILV
TAAPDHRPQPLLQQLAVGGHLVIPVGTVGQRLEVLHKTSTDLEMEKAIAVRFVPLQGHSYGF

Sequences:

>Translated_222_residues
MDLPPTNDFCADPTVALRQRMVKQQIIARGVNDPAVLAALQQVPRHRFVPDSLQNLAYADQPLTIGYGQTISQPYIVAYM
TEAAHLTPSSKVLEIGTGCGYQAAILAEIAQEVFTVEVVPELARQARDRLEALGYQNIHYKIGDGYQGWSEFAPYDAILV
TAAPDHRPQPLLQQLAVGGHLVIPVGTVGQRLEVLHKTSTDLEMEKAIAVRFVPLQGHSYGF
>Mature_222_residues
MDLPPTNDFCADPTVALRQRMVKQQIIARGVNDPAVLAALQQVPRHRFVPDSLQNLAYADQPLTIGYGQTISQPYIVAYM
TEAAHLTPSSKVLEIGTGCGYQAAILAEIAQEVFTVEVVPELARQARDRLEALGYQNIHYKIGDGYQGWSEFAPYDAILV
TAAPDHRPQPLLQQLAVGGHLVIPVGTVGQRLEVLHKTSTDLEMEKAIAVRFVPLQGHSYGF

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=171, Percent_Identity=35.0877192982456, Blast_Score=82, Evalue=3e-16,
Organism=Escherichia coli, GI1789100, Length=201, Percent_Identity=48.7562189054726, Blast_Score=180, Evalue=7e-47,
Organism=Caenorhabditis elegans, GI71983477, Length=189, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI193207222, Length=187, Percent_Identity=32.620320855615, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17981723, Length=199, Percent_Identity=32.1608040201005, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_SYNP2 (B1XQE1)

Other databases:

- EMBL:   CP000951
- RefSeq:   YP_001733939.1
- ProteinModelPortal:   B1XQE1
- SMR:   B1XQE1
- GeneID:   6057047
- GenomeReviews:   CP000951_GR
- KEGG:   syp:SYNPCC7002_A0678
- HOGENOM:   HBG699907
- OMA:   MGAKEDL
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 24356; Mature: 24356

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS01279 PCMT; PS00430 TONB_DEPENDENT_REC_1

Important sites: ACT_SITE 72-72

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLPPTNDFCADPTVALRQRMVKQQIIARGVNDPAVLAALQQVPRHRFVPDSLQNLAYAD
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCC
QPLTIGYGQTISQPYIVAYMTEAAHLTPSSKVLEIGTGCGYQAAILAEIAQEVFTVEVVP
CCEEECCCCCCCCCEEEEEEEHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
ELARQARDRLEALGYQNIHYKIGDGYQGWSEFAPYDAILVTAAPDHRPQPLLQQLAVGGH
HHHHHHHHHHHHCCCCCEEEEECCCCCCHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCE
LVIPVGTVGQRLEVLHKTSTDLEMEKAIAVRFVPLQGHSYGF
EEEECCCHHHHHHHHHHCCCCHHHHHEEEEEEEEECCCCCCC
>Mature Secondary Structure
MDLPPTNDFCADPTVALRQRMVKQQIIARGVNDPAVLAALQQVPRHRFVPDSLQNLAYAD
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCC
QPLTIGYGQTISQPYIVAYMTEAAHLTPSSKVLEIGTGCGYQAAILAEIAQEVFTVEVVP
CCEEECCCCCCCCCEEEEEEEHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
ELARQARDRLEALGYQNIHYKIGDGYQGWSEFAPYDAILVTAAPDHRPQPLLQQLAVGGH
HHHHHHHHHHHHCCCCCEEEEECCCCCCHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCE
LVIPVGTVGQRLEVLHKTSTDLEMEKAIAVRFVPLQGHSYGF
EEEECCCHHHHHHHHHHCCCCHHHHHEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA