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The map label for this gene is ddlB [C]

Identifier: 170077261

GI number: 170077261

Start: 668893

End: 670047

Strand: Reverse

Name: ddlB [C]

Synonym: SYNPCC7002_A0638

Alternate gene names: 170077261

Gene position: 670047-668893 (Counterclockwise)

Preceding gene: 170077263

Following gene: 170077260

Centisome position: 22.28

GC content: 47.1

Gene sequence:

>1155_bases
ATGATGGGATTTGTTTACGATTTCAGTCAACTACCAAACTACCTTGAAATTCTCCGGAAAAAGTTAAGAATTGCTGTAAT
TCATGGGGGTGACAAAGCAGCGCAGGGGGCTGTGATCTACCAAACCCACAATCCCCGCTCCACCAAAACCTACGAAGTGG
TAGCCCATGACATTGCCGCGGCCCTCGAAGATTTAGGGTTTCAGCACGTGTTTGTGTTGCCCGATGATGCGACATTAGGC
GATCGCCTCCGGAAACATCAAATTCATCTGGCTTGGCTGAATACTGGTGGGGTGCAGGGTTACAATCCCGTCTGCCACAC
CCCGGCAATTTTAGAAATGTTGGGAGTACCCTATATCGGCCATAACCCTTTAAATAGCTCGATTTTAGATAATAAACACA
GCTTTAAACGGGAACTGCAAGGGCTCGGTTTTCCCACTGCGCCGTTCATGATTTGGCATCCAGCCCAGGGGAAATTTACT
GCCAAGGACAACGCTCGTTTCCAGCAGACTTTTGGCGATTACCAGGGCAGTTTTCTGGTTAAACCCGTGTCTGGTCGTGC
CTCTTTGAACATTCATTTTGTGGATCAGCCCCAGGATCTCGGTGAGGCGATCGCCAACATACATCAGCAAACCCACAACA
CCGCTTTGATTGAACCCTTCTTAACCGGGCGCGAATTTTGTGTCTCTGTGTGTGGCTATGTGCGCCATCGTCAAGGGCAG
TTTGAAAAATTAGAATCTCCCTTTACGTTTTCGATTTTAGAGCGTCTTTTAGAAGCCGATGAAAAGATTTTTACCTCGAT
GGATAAAAAGGCAATTACAGGCCAAAGAATTCGCTTTTTAGATGACAGTGACCCAGCCAAAAAAAAGCTAGCAAAACTCG
CCCAACAAGTCTACTGGGAATTTAGTTTAAATGCCTTAATTCGTATCGATGCCCGGATGGATGCGATGGGAAATATCCAA
ATTTTAGAGGCTAATCCCAAACCCGATCTTAAGCGGCCCGCTGCCAACGTCACCAGCTTGGTTGCCCAGGGACTATCAGA
AGAGGGAATGACCTACGAGGATTTAATTTTGTCGCTGATTAGCGATCGCCTGGATTATTTATTGACCAACCACGCCCAGA
TTATCCCCCACATTACGGCCCTGCTTAATCCCTAA

Upstream 100 bases:

>100_bases
TACTTTTAAAGACTCAATCTCTTTTTTTCTGCCTGCCATATCTTTCTCAGGGATCCTCTAAAATAACAAAACTGCTCAGA
ATTGTGGGAAAGTCACGTTA

Downstream 100 bases:

>100_bases
AGGTGCTTTTCTCCCAAGGCTCAAGGGGACTTCATAAATCTATTTACATTGGGGGCGATCGCCTGCTATATAATTAGACT
CTGTTCGGTTTTATCGGGGA

Product: hypothetical protein

Products: ADP; D-Alanyl-D-alanine; H+; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MMGFVYDFSQLPNYLEILRKKLRIAVIHGGDKAAQGAVIYQTHNPRSTKTYEVVAHDIAAALEDLGFQHVFVLPDDATLG
DRLRKHQIHLAWLNTGGVQGYNPVCHTPAILEMLGVPYIGHNPLNSSILDNKHSFKRELQGLGFPTAPFMIWHPAQGKFT
AKDNARFQQTFGDYQGSFLVKPVSGRASLNIHFVDQPQDLGEAIANIHQQTHNTALIEPFLTGREFCVSVCGYVRHRQGQ
FEKLESPFTFSILERLLEADEKIFTSMDKKAITGQRIRFLDDSDPAKKKLAKLAQQVYWEFSLNALIRIDARMDAMGNIQ
ILEANPKPDLKRPAANVTSLVAQGLSEEGMTYEDLILSLISDRLDYLLTNHAQIIPHITALLNP

Sequences:

>Translated_384_residues
MMGFVYDFSQLPNYLEILRKKLRIAVIHGGDKAAQGAVIYQTHNPRSTKTYEVVAHDIAAALEDLGFQHVFVLPDDATLG
DRLRKHQIHLAWLNTGGVQGYNPVCHTPAILEMLGVPYIGHNPLNSSILDNKHSFKRELQGLGFPTAPFMIWHPAQGKFT
AKDNARFQQTFGDYQGSFLVKPVSGRASLNIHFVDQPQDLGEAIANIHQQTHNTALIEPFLTGREFCVSVCGYVRHRQGQ
FEKLESPFTFSILERLLEADEKIFTSMDKKAITGQRIRFLDDSDPAKKKLAKLAQQVYWEFSLNALIRIDARMDAMGNIQ
ILEANPKPDLKRPAANVTSLVAQGLSEEGMTYEDLILSLISDRLDYLLTNHAQIIPHITALLNP
>Mature_384_residues
MMGFVYDFSQLPNYLEILRKKLRIAVIHGGDKAAQGAVIYQTHNPRSTKTYEVVAHDIAAALEDLGFQHVFVLPDDATLG
DRLRKHQIHLAWLNTGGVQGYNPVCHTPAILEMLGVPYIGHNPLNSSILDNKHSFKRELQGLGFPTAPFMIWHPAQGKFT
AKDNARFQQTFGDYQGSFLVKPVSGRASLNIHFVDQPQDLGEAIANIHQQTHNTALIEPFLTGREFCVSVCGYVRHRQGQ
FEKLESPFTFSILERLLEADEKIFTSMDKKAITGQRIRFLDDSDPAKKKLAKLAQQVYWEFSLNALIRIDARMDAMGNIQ
ILEANPKPDLKRPAANVTSLVAQGLSEEGMTYEDLILSLISDRLDYLLTNHAQIIPHITALLNP

Specific function: Could be involved in the biosynthesis of a cell wall component [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011095
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C [H]

EC number: 6.3.2.4 [C]

Molecular weight: Translated: 43109; Mature: 43109

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMGFVYDFSQLPNYLEILRKKLRIAVIHGGDKAAQGAVIYQTHNPRSTKTYEVVAHDIAA
CCCCCCCHHHCCHHHHHHHHHCEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
ALEDLGFQHVFVLPDDATLGDRLRKHQIHLAWLNTGGVQGYNPVCHTPAILEMLGVPYIG
HHHHCCCCEEEEECCCCCHHHHHHHCEEEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCC
HNPLNSSILDNKHSFKRELQGLGFPTAPFMIWHPAQGKFTAKDNARFQQTFGDYQGSFLV
CCCCCCHHHCCHHHHHHHHHHCCCCCCCEEEEECCCCCEECCCCCHHHHHHHCCCCCEEE
KPVSGRASLNIHFVDQPQDLGEAIANIHQQTHNTALIEPFLTGREFCVSVCGYVRHRQGQ
EECCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCC
FEKLESPFTFSILERLLEADEKIFTSMDKKAITGQRIRFLDDSDPAKKKLAKLAQQVYWE
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHH
FSLNALIRIDARMDAMGNIQILEANPKPDLKRPAANVTSLVAQGLSEEGMTYEDLILSLI
CCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHH
SDRLDYLLTNHAQIIPHITALLNP
HHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure
MMGFVYDFSQLPNYLEILRKKLRIAVIHGGDKAAQGAVIYQTHNPRSTKTYEVVAHDIAA
CCCCCCCHHHCCHHHHHHHHHCEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
ALEDLGFQHVFVLPDDATLGDRLRKHQIHLAWLNTGGVQGYNPVCHTPAILEMLGVPYIG
HHHHCCCCEEEEECCCCCHHHHHHHCEEEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCC
HNPLNSSILDNKHSFKRELQGLGFPTAPFMIWHPAQGKFTAKDNARFQQTFGDYQGSFLV
CCCCCCHHHCCHHHHHHHHHHCCCCCCCEEEEECCCCCEECCCCCHHHHHHHCCCCCEEE
KPVSGRASLNIHFVDQPQDLGEAIANIHQQTHNTALIEPFLTGREFCVSVCGYVRHRQGQ
EECCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCC
FEKLESPFTFSILERLLEADEKIFTSMDKKAITGQRIRFLDDSDPAKKKLAKLAQQVYWE
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHH
FSLNALIRIDARMDAMGNIQILEANPKPDLKRPAANVTSLVAQGLSEEGMTYEDLILSLI
CCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHH
SDRLDYLLTNHAQIIPHITALLNP
HHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1018 [C]

Specific activity: 31

Km value (mM): 15 {D-norleucine}} 6 {D-Phe}} 40 {ATP}} 2.5 {ATP}} 2.4 {ATP}} 0.179 {ATP}} 0.161 {ATP}} 0.055 {ATP}} 0.049 {ATP}} 0.033 {ATP}} 0.008 {ATP}} 9 {D-Met}} 86 {D-Ala}} 70.3 {D-Ala}} 60.4 {D-Ala}} 57.5 {D-

Substrates: D-Alanine; ATP [C]

Specific reaction: (2) D-Alanine + ATP <==> ADP + D-Alanyl-D-alanine + H+ + Phosphate [C]

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: [1 (S)-Aminoethyl] [(2RS) 2-carboxy-1-octyl] phosphinic acid; Aminoalkyl phosphinate; D-Ala-D-2-hydroxy; D-Ala-D-Ala; D-Ala-D-Alaphosphinate; D-Cycloserine; Gly; Gly-Gly; Phosphinates; Phosphonate dipeptide analogs [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]