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The map label for this gene is amiC [H]

Identifier: 170077203

GI number: 170077203

Start: 603209

End: 605026

Strand: Direct

Name: amiC [H]

Synonym: SYNPCC7002_A0578

Alternate gene names: 170077203

Gene position: 603209-605026 (Clockwise)

Preceding gene: 170077202

Following gene: 170077204

Centisome position: 20.05

GC content: 54.13

Gene sequence:

>1818_bases
GTGAGATTTTTCTGGTTTTTTCTGACGCTACTGACGCTATCCACTTGGCAACTACCGGCTTGGGCAGGTCAATTGGTCTT
TTGGGAATTTAATACCCAACGACGTCAATTGCAGTTTCGCACCAATGAACGGGTGCAGCCCGAAGCGCAACTGATCCCAG
ATCCCAGCCGTTTGGTGATTGATTTACCGGGCACTGTCCTGGGAAGCTCTACGGTCAATCGCAGCTATGGCGGCAAAATT
ACGAGCGTACGGCTAGGTCAGTTTGCGCCAGATGTCACACGGTTGGTGGTGGAATTAGCCCCTGGCTATACCTTAGATCC
TGAGGCAATTCGTTTTGAAGGGCGATCGCCGACGGATTGGGTTGTGAATTTACCAGAACCTGAACTCATTCCCGTGGCCC
CAGTAGCTCCCAGTACCCCAGAAATTTTTAACAATTCTACCAATCCTGGAAATCCCAGTAACGCCGCTCCAGAAGAAGCT
TTTTTTAATAACTTGGTGCGGGTGACCCAACAGGGATTCCTCGTCAACCTGGATCCCCGGCCCGGTAGTCGCATCACCAC
CGACCAAGATGGCGATCGCCTTACCTTTACCCTGCGGGATTTGCAATTGCCCCGGAGCTTAGAAAATCAGACCCTAGATG
TTAACCGCTACAAAGTCCGCCAAATCAAATTTGAGCAACCCAGCCGTACCGAAGCCCGCATTACCTTAAATTTAGAACCC
GGTAGCCCTCGGTGGCGCGCGCTGTATACCACCTACGGTGATGGCGGTTTGGTACTGCTTCCGGATGGGGGCACCAGTAG
CCTAGGGGGAAGAGAAACCGGGGCGATCACCGTCCCCACCCAACAATCAGTCGCCACAGCTCAACCCCAACAAACCATCG
TTGAAGCCCTAGAACTTGCCCGCAACAGTACCCAGTTAGTCATCCGTGGCAGCGATCGCCTCCAGGCCGATGGTGGTTGG
AATCGTAACGCCGGCTTCTACCAAATTCGCATTAACAACGCCCGCCTTGCCCAACCTGTGCGTGGCCCCCAGTGGGGGGG
AGATAGTCCCATTTCCCGCATTGCCATCCGCCAGGAAACGGCCAATACGGTGCTCGTCCAGATTGAACCCGCCGAGGGGG
TACAAATTGGAAGTCTCAGTCAACCCAATGCCCAGTTTCTTGCCCTAGACCTCAATCGTCCCCAAACGGCAAGCCCCCTG
TTTGTGCCGCCGGCCCCAACGGCACCGCCCCCCACAACCCAAGCGGTACTGCCAAATTTTCGCCCGCCCCGGAGCAATAT
TATGGTCATGATCGACCCCGGCCATGGTGGTCGTGATCCCGGTGCTGTGGGGATTGGTGGCCTACAGGAAAAAAACGTGA
TTCTCCCCATCTCCCAGGAAGTAGCCCGAATTCTGCAACAAAGTGGCATCACTGCCCGAATGACCCGGGATACGGATTAT
TTCGTCAGCCTAGAAGGACGTACTCAACTGGCTAACAATGCCAACGCCGATTTATTCGTAAGTATCCATGCCAACGCCAT
CAGCATGAGTCGCCCTGATGTGAACGGTCTAGAGGTGTATTATCACCAGACCGGGAAGACTTTAGCCGAGTCTATTCATC
GCAATATTATGCGGCGGATTCAAATGCGAGACCGGGGGGTGAGAACGGCGCGCTTTTATGTATTGCGTAATACATCCATG
CCTTCAGTGCTCGTGGAAACAGGTTTTGTCACGGGTCGGGAAGATGCTCCCCGCTTGAATGATCCTGCATTTCGGAGCCA
AATGGCCCAGGCGATCGCCGCTGGCATCCTGGAATATATCCAAAACAATGTGCGCTAG

Upstream 100 bases:

>100_bases
CCTTGGGAGAAGGTAGGAAGAGGTTTTATCTCTATCCACCGGAATTGTTCTATATCTTGAGGGCGTGGCATTTTCCCTTG
TGAGTCGAGGATTTTTAACT

Downstream 100 bases:

>100_bases
CCCAGGGGGTTGCCAGAAAATCAGTAATTCGCCACAGAAATAAGCGCAAATACCTTAAAACTGCGCTAATCTTTGTTTAA
TTTATTTGGTGTGAACGAGA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Cell wall hydrolase; Cell wall-associated polypeptide CWBP49; CWBP49; Major autolysin [H]

Number of amino acids: Translated: 605; Mature: 605

Protein sequence:

>605_residues
MRFFWFFLTLLTLSTWQLPAWAGQLVFWEFNTQRRQLQFRTNERVQPEAQLIPDPSRLVIDLPGTVLGSSTVNRSYGGKI
TSVRLGQFAPDVTRLVVELAPGYTLDPEAIRFEGRSPTDWVVNLPEPELIPVAPVAPSTPEIFNNSTNPGNPSNAAPEEA
FFNNLVRVTQQGFLVNLDPRPGSRITTDQDGDRLTFTLRDLQLPRSLENQTLDVNRYKVRQIKFEQPSRTEARITLNLEP
GSPRWRALYTTYGDGGLVLLPDGGTSSLGGRETGAITVPTQQSVATAQPQQTIVEALELARNSTQLVIRGSDRLQADGGW
NRNAGFYQIRINNARLAQPVRGPQWGGDSPISRIAIRQETANTVLVQIEPAEGVQIGSLSQPNAQFLALDLNRPQTASPL
FVPPAPTAPPPTTQAVLPNFRPPRSNIMVMIDPGHGGRDPGAVGIGGLQEKNVILPISQEVARILQQSGITARMTRDTDY
FVSLEGRTQLANNANADLFVSIHANAISMSRPDVNGLEVYYHQTGKTLAESIHRNIMRRIQMRDRGVRTARFYVLRNTSM
PSVLVETGFVTGREDAPRLNDPAFRSQMAQAIAAGILEYIQNNVR

Sequences:

>Translated_605_residues
MRFFWFFLTLLTLSTWQLPAWAGQLVFWEFNTQRRQLQFRTNERVQPEAQLIPDPSRLVIDLPGTVLGSSTVNRSYGGKI
TSVRLGQFAPDVTRLVVELAPGYTLDPEAIRFEGRSPTDWVVNLPEPELIPVAPVAPSTPEIFNNSTNPGNPSNAAPEEA
FFNNLVRVTQQGFLVNLDPRPGSRITTDQDGDRLTFTLRDLQLPRSLENQTLDVNRYKVRQIKFEQPSRTEARITLNLEP
GSPRWRALYTTYGDGGLVLLPDGGTSSLGGRETGAITVPTQQSVATAQPQQTIVEALELARNSTQLVIRGSDRLQADGGW
NRNAGFYQIRINNARLAQPVRGPQWGGDSPISRIAIRQETANTVLVQIEPAEGVQIGSLSQPNAQFLALDLNRPQTASPL
FVPPAPTAPPPTTQAVLPNFRPPRSNIMVMIDPGHGGRDPGAVGIGGLQEKNVILPISQEVARILQQSGITARMTRDTDY
FVSLEGRTQLANNANADLFVSIHANAISMSRPDVNGLEVYYHQTGKTLAESIHRNIMRRIQMRDRGVRTARFYVLRNTSM
PSVLVETGFVTGREDAPRLNDPAFRSQMAQAIAAGILEYIQNNVR
>Mature_605_residues
MRFFWFFLTLLTLSTWQLPAWAGQLVFWEFNTQRRQLQFRTNERVQPEAQLIPDPSRLVIDLPGTVLGSSTVNRSYGGKI
TSVRLGQFAPDVTRLVVELAPGYTLDPEAIRFEGRSPTDWVVNLPEPELIPVAPVAPSTPEIFNNSTNPGNPSNAAPEEA
FFNNLVRVTQQGFLVNLDPRPGSRITTDQDGDRLTFTLRDLQLPRSLENQTLDVNRYKVRQIKFEQPSRTEARITLNLEP
GSPRWRALYTTYGDGGLVLLPDGGTSSLGGRETGAITVPTQQSVATAQPQQTIVEALELARNSTQLVIRGSDRLQADGGW
NRNAGFYQIRINNARLAQPVRGPQWGGDSPISRIAIRQETANTVLVQIEPAEGVQIGSLSQPNAQFLALDLNRPQTASPL
FVPPAPTAPPPTTQAVLPNFRPPRSNIMVMIDPGHGGRDPGAVGIGGLQEKNVILPISQEVARILQQSGITARMTRDTDY
FVSLEGRTQLANNANADLFVSIHANAISMSRPDVNGLEVYYHQTGKTLAESIHRNIMRRIQMRDRGVRTARFYVLRNTSM
PSVLVETGFVTGREDAPRLNDPAFRSQMAQAIAAGILEYIQNNVR

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. Has a high affinity for teich

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=232, Percent_Identity=32.3275862068966, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI87082163, Length=247, Percent_Identity=29.5546558704453, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1790611, Length=225, Percent_Identity=30.2222222222222, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007253
- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3; PF04122 CW_binding_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 66889; Mature: 66889

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFFWFFLTLLTLSTWQLPAWAGQLVFWEFNTQRRQLQFRTNERVQPEAQLIPDPSRLVI
CHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCCHHHCCCCCCEEEE
DLPGTVLGSSTVNRSYGGKITSVRLGQFAPDVTRLVVELAPGYTLDPEAIRFEGRSPTDW
ECCCCEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCHHEEECCCCCCCE
VVNLPEPELIPVAPVAPSTPEIFNNSTNPGNPSNAAPEEAFFNNLVRVTQQGFLVNLDPR
EEECCCCCEEEECCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCC
PGSRITTDQDGDRLTFTLRDLQLPRSLENQTLDVNRYKVRQIKFEQPSRTEARITLNLEP
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEECCEEEEEEEEECCCCCCEEEEEEEECC
GSPRWRALYTTYGDGGLVLLPDGGTSSLGGRETGAITVPTQQSVATAQPQQTIVEALELA
CCCCEEEEEEEECCCCEEEEECCCCCCCCCCCCCEEECCCCCCHHHCCHHHHHHHHHHHC
RNSTQLVIRGSDRLQADGGWNRNAGFYQIRINNARLAQPVRGPQWGGDSPISRIAIRQET
CCCCEEEEECCCEEECCCCCCCCCCEEEEEECCCEECCCCCCCCCCCCCCHHHEEEEECC
ANTVLVQIEPAEGVQIGSLSQPNAQFLALDLNRPQTASPLFVPPAPTAPPPTTQAVLPNF
CCEEEEEEECCCCEEECCCCCCCCEEEEEECCCCCCCCCEEECCCCCCCCCCCHHHCCCC
RPPRSNIMVMIDPGHGGRDPGAVGIGGLQEKNVILPISQEVARILQQSGITARMTRDTDY
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCEEEEECCCEE
FVSLEGRTQLANNANADLFVSIHANAISMSRPDVNGLEVYYHQTGKTLAESIHRNIMRRI
EEEECCCHHHCCCCCCEEEEEEECCEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHH
QMRDRGVRTARFYVLRNTSMPSVLVETGFVTGREDAPRLNDPAFRSQMAQAIAAGILEYI
HHHHCCCEEEEEEEEECCCCCEEEEEECCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QNNVR
HHCCC
>Mature Secondary Structure
MRFFWFFLTLLTLSTWQLPAWAGQLVFWEFNTQRRQLQFRTNERVQPEAQLIPDPSRLVI
CHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCCHHHCCCCCCEEEE
DLPGTVLGSSTVNRSYGGKITSVRLGQFAPDVTRLVVELAPGYTLDPEAIRFEGRSPTDW
ECCCCEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCHHEEECCCCCCCE
VVNLPEPELIPVAPVAPSTPEIFNNSTNPGNPSNAAPEEAFFNNLVRVTQQGFLVNLDPR
EEECCCCCEEEECCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCC
PGSRITTDQDGDRLTFTLRDLQLPRSLENQTLDVNRYKVRQIKFEQPSRTEARITLNLEP
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEECCEEEEEEEEECCCCCCEEEEEEEECC
GSPRWRALYTTYGDGGLVLLPDGGTSSLGGRETGAITVPTQQSVATAQPQQTIVEALELA
CCCCEEEEEEEECCCCEEEEECCCCCCCCCCCCCEEECCCCCCHHHCCHHHHHHHHHHHC
RNSTQLVIRGSDRLQADGGWNRNAGFYQIRINNARLAQPVRGPQWGGDSPISRIAIRQET
CCCCEEEEECCCEEECCCCCCCCCCEEEEEECCCEECCCCCCCCCCCCCCHHHEEEEECC
ANTVLVQIEPAEGVQIGSLSQPNAQFLALDLNRPQTASPLFVPPAPTAPPPTTQAVLPNF
CCEEEEEEECCCCEEECCCCCCCCEEEEEECCCCCCCCCEEECCCCCCCCCCCHHHCCCC
RPPRSNIMVMIDPGHGGRDPGAVGIGGLQEKNVILPISQEVARILQQSGITARMTRDTDY
CCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCEEEEECCCEE
FVSLEGRTQLANNANADLFVSIHANAISMSRPDVNGLEVYYHQTGKTLAESIHRNIMRRI
EEEECCCHHHCCCCCCEEEEEEECCEEECCCCCCCCEEEEEECCCHHHHHHHHHHHHHHH
QMRDRGVRTARFYVLRNTSMPSVLVETGFVTGREDAPRLNDPAFRSQMAQAIAAGILEYI
HHHHCCCEEEEEEEEECCCCCEEEEEECCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QNNVR
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1357079; 1682302; 9384377 [H]