Click here to switch to the map view.

The map label for this gene is priA [H]

Identifier: 170076993

GI number: 170076993

Start: 390377

End: 392788

Strand: Direct

Name: priA [H]

Synonym: SYNPCC7002_A0365

Alternate gene names: 170076993

Gene position: 390377-392788 (Clockwise)

Preceding gene: 170076991

Following gene: 170077003

Centisome position: 12.98

GC content: 53.23

Gene sequence:

>2412_bases
TTGGATTGGCTTGAGGTCTTAGTTGATTGTGGTTATACTCAGGGGCTTTTTACCTATGGAGTGCCGCTAGAATTGGCGGT
GGTGCCAGGGGATATCGTATTGGTTGGGTTTGGGGCGCAACAGGTGGGAGCGATCGCCATTCGCTTCATTGCAAAGCTCC
CCCAGGGACTAGATCCTGAGCAAATCCGTCCGGTGCAGGGGATCATCGCGCGGGGCTTTTTTTCGCCCCACTATTGGCAA
CTTTTGTTGCGGGTTGCCCAGGCCTATCAAACGGATATGATGACGGTGGTACGAATGGCTTTGCCGCCGGGTTTATTGCG
GCGATCGCAACCCCGTGTTCGGCTTTTGAAAACCGATCTCGCCTCCATCGACATCGACTTTTTACCACCGACGGCCCAAC
AACTTTTCCAGTTACTCCAGCGGGGATCAACCCAGGACTACAGTGTGAAGTACCTCCAACGACAGATTAAGTCGGTCTAT
CGCGGGATACAGGAATTGGAAAAGGCGGGCCTCGCAGAACGATATTTGGCGACGCCCCAAGTCATCCACGGGCAGCGGCA
AAAGTGGGTGAGCTACGTGAGCGAACGGGAAGGGCTCACCAAACGGCAACGGGAAATTTTGCTGGTGTTGCGCCATGAAG
GGGGCGAAATGTGGCTCTCGGAATTGGTGGGGGTGGCGAAAACAACGGCGACAACGGTGACGAAAATGGCAAAGGTCGGT
TGTGTGGCGATCGCCGAACGGGAAAAATTACGCTTACACCAGTCGCCGTCCCAGGGCGTAGACCCTGCCAAAACATTAAC
TAATGCCCAACAAGCAGCTCTAGAAACCATCAAAAAAATCCAGGGTTACGGGGAAGTTCTGTTACATGGGGTGACGGGTT
CAGGGAAAACGGAGGTTTATCTCCAGGCGATCGCCCCCCTGTTATCTCAACAAAAATCCGCCCTTGTCCTCGTGCCAGAA
ATTGGCCTCACACCGCAACTAATGGATCGATTTCGGTCACGGTTTGGCGATCGCGTTTTGACTTACCACAGCGGTTTATC
AGCAGGAGAACGCTACGATACATGGCGGCAAATGTTGCGCCCCGACAGCCAAATTATCATCGGCACCCGTTCCGCTGTTT
TTGCGCCCCTGCCCAATTTAGGGATGATCATCCTCGACGAAGAACACGACAGTAGTTACAAACAGGATCAGCCCGCCCCC
ACCTACCATGCCCGCACCGTCGCCCGGTGGCGATCGCAGCAGGAAAATTGCCCATTGCTTCTTGGTTCTGCCACCCCAGC
CCTGGACACTTGGGTAGAATTTCAAACCGCCCAAAACTCAAATTACTATTACATTTCCCTGCCTGAGCGGGTGTATGCTC
GTCCGCTGCCCCCCGTGGAAATTGTTGATATGCGCCATGAACTGCGCGTCGGCAACCGCTCCCTTTTTAGTCAGTCCCTC
CGGCAGGCCCTCGAACGTATTACCCAAACCCACGAACAGGGGATTTTATTTATTGCCCGTCGCGGTCACAGTACCTTTGT
TTCCTGTCGCAGTTGCGGTTATGTGATGGAATGCCCCCATTGTGATGTGTCCCTCTCCTACCATTACGTTCAGGAGGGCA
GTTTACCGCTATTGCGTTGCCATTATTGCGATCATAGCCAGATCCAACCCAAACATTGCCCAAGCTGTAATTCCCCCTAT
TTCAAGTTTTTCGGGAACGGCACCCAAAAAGTTTTCCAGGCGCTGCAAAAAGAATTTCCTCAGTTACGCTGTCTGCGATT
TGATAGTGATACCACCCGTCGAAAAGGGGCGCACCGCGACTTATTGGGGCAATTTGCCCGGGGCGAGGCGGATATTTTAC
TAGGTACCCAGATGCTCACGAAGGGTTTGGATGTGGCTCAAGTGACCCTTGTGGGGGTGATTGCCGCCGATGGTCTATTA
CACCAAAGTGATTATCGGGCTGCGGAACGCACCTTTCAAACCCTAACCCAGGTGGCAGGTCGGGCGGGTCGGGGTGATGA
ACCGGGCCAAGTGATCATTCAGACCTATTCCCCAGAGCATCCGGTGATTCAAGCGGTAAAAACCCATGATTACCTGACCT
TTGCCGAGCGGGAAATAAGTCAACGGCAGGAATTGGACTATCCGCCCTTTGGCAAATTAATTTTGCTGCGCTTTAGTGGA
GAAAACCACGATCAGGTGCGCTACACCGCCGAGGCGATCGCCGAAAAATGTTTCCCACTGCTCGAACCCGAAGACGAACT
TCTTGGCCCCGTGCCAGCGAATATTCTGCGAGTGGCACGGCGTTACCGTTGGCAGGTGGTTTTAAAATTTCCCCAGCGCA
AAACCGAGATCCCCGATTTGACTTTTTTGCGGGAATTTTGCCCGCGTCCGGTAAGCCTTTCGATTAATGTTGATCCGTTG
TACATCGATTAG

Upstream 100 bases:

>100_bases
TGTATATAGAGAAAAATAATTGCCCTTATTTGAATTGATAAAAAATCATGGGGTATAGTGCTTTTGCAGTGAGTTTTGGG
AATCTTAGGAGATTGGGGAT

Downstream 100 bases:

>100_bases
CCATTCAGCGAAAAATTTTTTAAAACTAAGCCGTAATTTTCATGCTGATATCGAGCCACGGCGCACGGGTAATCGTCGCA
CTGGTAGAAATGTAATCGAT

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 803; Mature: 803

Protein sequence:

>803_residues
MDWLEVLVDCGYTQGLFTYGVPLELAVVPGDIVLVGFGAQQVGAIAIRFIAKLPQGLDPEQIRPVQGIIARGFFSPHYWQ
LLLRVAQAYQTDMMTVVRMALPPGLLRRSQPRVRLLKTDLASIDIDFLPPTAQQLFQLLQRGSTQDYSVKYLQRQIKSVY
RGIQELEKAGLAERYLATPQVIHGQRQKWVSYVSEREGLTKRQREILLVLRHEGGEMWLSELVGVAKTTATTVTKMAKVG
CVAIAEREKLRLHQSPSQGVDPAKTLTNAQQAALETIKKIQGYGEVLLHGVTGSGKTEVYLQAIAPLLSQQKSALVLVPE
IGLTPQLMDRFRSRFGDRVLTYHSGLSAGERYDTWRQMLRPDSQIIIGTRSAVFAPLPNLGMIILDEEHDSSYKQDQPAP
TYHARTVARWRSQQENCPLLLGSATPALDTWVEFQTAQNSNYYYISLPERVYARPLPPVEIVDMRHELRVGNRSLFSQSL
RQALERITQTHEQGILFIARRGHSTFVSCRSCGYVMECPHCDVSLSYHYVQEGSLPLLRCHYCDHSQIQPKHCPSCNSPY
FKFFGNGTQKVFQALQKEFPQLRCLRFDSDTTRRKGAHRDLLGQFARGEADILLGTQMLTKGLDVAQVTLVGVIAADGLL
HQSDYRAAERTFQTLTQVAGRAGRGDEPGQVIIQTYSPEHPVIQAVKTHDYLTFAEREISQRQELDYPPFGKLILLRFSG
ENHDQVRYTAEAIAEKCFPLLEPEDELLGPVPANILRVARRYRWQVVLKFPQRKTEIPDLTFLREFCPRPVSLSINVDPL
YID

Sequences:

>Translated_803_residues
MDWLEVLVDCGYTQGLFTYGVPLELAVVPGDIVLVGFGAQQVGAIAIRFIAKLPQGLDPEQIRPVQGIIARGFFSPHYWQ
LLLRVAQAYQTDMMTVVRMALPPGLLRRSQPRVRLLKTDLASIDIDFLPPTAQQLFQLLQRGSTQDYSVKYLQRQIKSVY
RGIQELEKAGLAERYLATPQVIHGQRQKWVSYVSEREGLTKRQREILLVLRHEGGEMWLSELVGVAKTTATTVTKMAKVG
CVAIAEREKLRLHQSPSQGVDPAKTLTNAQQAALETIKKIQGYGEVLLHGVTGSGKTEVYLQAIAPLLSQQKSALVLVPE
IGLTPQLMDRFRSRFGDRVLTYHSGLSAGERYDTWRQMLRPDSQIIIGTRSAVFAPLPNLGMIILDEEHDSSYKQDQPAP
TYHARTVARWRSQQENCPLLLGSATPALDTWVEFQTAQNSNYYYISLPERVYARPLPPVEIVDMRHELRVGNRSLFSQSL
RQALERITQTHEQGILFIARRGHSTFVSCRSCGYVMECPHCDVSLSYHYVQEGSLPLLRCHYCDHSQIQPKHCPSCNSPY
FKFFGNGTQKVFQALQKEFPQLRCLRFDSDTTRRKGAHRDLLGQFARGEADILLGTQMLTKGLDVAQVTLVGVIAADGLL
HQSDYRAAERTFQTLTQVAGRAGRGDEPGQVIIQTYSPEHPVIQAVKTHDYLTFAEREISQRQELDYPPFGKLILLRFSG
ENHDQVRYTAEAIAEKCFPLLEPEDELLGPVPANILRVARRYRWQVVLKFPQRKTEIPDLTFLREFCPRPVSLSINVDPL
YID
>Mature_803_residues
MDWLEVLVDCGYTQGLFTYGVPLELAVVPGDIVLVGFGAQQVGAIAIRFIAKLPQGLDPEQIRPVQGIIARGFFSPHYWQ
LLLRVAQAYQTDMMTVVRMALPPGLLRRSQPRVRLLKTDLASIDIDFLPPTAQQLFQLLQRGSTQDYSVKYLQRQIKSVY
RGIQELEKAGLAERYLATPQVIHGQRQKWVSYVSEREGLTKRQREILLVLRHEGGEMWLSELVGVAKTTATTVTKMAKVG
CVAIAEREKLRLHQSPSQGVDPAKTLTNAQQAALETIKKIQGYGEVLLHGVTGSGKTEVYLQAIAPLLSQQKSALVLVPE
IGLTPQLMDRFRSRFGDRVLTYHSGLSAGERYDTWRQMLRPDSQIIIGTRSAVFAPLPNLGMIILDEEHDSSYKQDQPAP
TYHARTVARWRSQQENCPLLLGSATPALDTWVEFQTAQNSNYYYISLPERVYARPLPPVEIVDMRHELRVGNRSLFSQSL
RQALERITQTHEQGILFIARRGHSTFVSCRSCGYVMECPHCDVSLSYHYVQEGSLPLLRCHYCDHSQIQPKHCPSCNSPY
FKFFGNGTQKVFQALQKEFPQLRCLRFDSDTTRRKGAHRDLLGQFARGEADILLGTQMLTKGLDVAQVTLVGVIAADGLL
HQSDYRAAERTFQTLTQVAGRAGRGDEPGQVIIQTYSPEHPVIQAVKTHDYLTFAEREISQRQELDYPPFGKLILLRFSG
ENHDQVRYTAEAIAEKCFPLLEPEDELLGPVPANILRVARRYRWQVVLKFPQRKTEIPDLTFLREFCPRPVSLSINVDPL
YID

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=612, Percent_Identity=38.0718954248366, Blast_Score=374, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 90819; Mature: 90819

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDWLEVLVDCGYTQGLFTYGVPLELAVVPGDIVLVGFGAQQVGAIAIRFIAKLPQGLDPE
CCHHHHHHHCCCCCCCEEECCCEEEEEECCCEEEEECCHHHHHHHHHHHHHHCCCCCCHH
QIRPVQGIIARGFFSPHYWQLLLRVAQAYQTDMMTVVRMALPPGLLRRSQPRVRLLKTDL
HHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHH
ASIDIDFLPPTAQQLFQLLQRGSTQDYSVKYLQRQIKSVYRGIQELEKAGLAERYLATPQ
HHEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCH
VIHGQRQKWVSYVSEREGLTKRQREILLVLRHEGGEMWLSELVGVAKTTATTVTKMAKVG
HHHCHHHHHHHHHHHHCCHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
CVAIAEREKLRLHQSPSQGVDPAKTLTNAQQAALETIKKIQGYGEVLLHGVTGSGKTEVY
HHEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHH
LQAIAPLLSQQKSALVLVPEIGLTPQLMDRFRSRFGDRVLTYHSGLSAGERYDTWRQMLR
HHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHC
PDSQIIIGTRSAVFAPLPNLGMIILDEEHDSSYKQDQPAPTYHARTVARWRSQQENCPLL
CCCEEEEEECCHHCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEE
LGSATPALDTWVEFQTAQNSNYYYISLPERVYARPLPPVEIVDMRHELRVGNRSLFSQSL
EECCCCHHHHHHEEEECCCCCEEEEECCHHHHCCCCCCHHHHHCHHHHHCCCHHHHHHHH
RQALERITQTHEQGILFIARRGHSTFVSCRSCGYVMECPHCDVSLSYHYVQEGSLPLLRC
HHHHHHHHHHHCCCEEEEEECCCCCEEEHHCCCCEEECCCCCEEEEEEEEECCCCCEEEE
HYCDHSQIQPKHCPSCNSPYFKFFGNGTQKVFQALQKEFPQLRCLRFDSDTTRRKGAHRD
ECCCCCCCCCCCCCCCCCCHHEECCCCHHHHHHHHHHHCCCEEEEEECCCHHHHCCCHHH
LLGQFARGEADILLGTQMLTKGLDVAQVTLVGVIAADGLLHQSDYRAAERTFQTLTQVAG
HHHHHHCCCCCEEECHHHHHCCCCHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHC
RAGRGDEPGQVIIQTYSPEHPVIQAVKTHDYLTFAEREISQRQELDYPPFGKLILLRFSG
CCCCCCCCCEEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECC
ENHDQVRYTAEAIAEKCFPLLEPEDELLGPVPANILRVARRYRWQVVLKFPQRKTEIPDL
CCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHEEEEEECCCCCCCCCCH
TFLREFCPRPVSLSINVDPLYID
HHHHHHCCCCEEEEEECCCEEEC
>Mature Secondary Structure
MDWLEVLVDCGYTQGLFTYGVPLELAVVPGDIVLVGFGAQQVGAIAIRFIAKLPQGLDPE
CCHHHHHHHCCCCCCCEEECCCEEEEEECCCEEEEECCHHHHHHHHHHHHHHCCCCCCHH
QIRPVQGIIARGFFSPHYWQLLLRVAQAYQTDMMTVVRMALPPGLLRRSQPRVRLLKTDL
HHCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHH
ASIDIDFLPPTAQQLFQLLQRGSTQDYSVKYLQRQIKSVYRGIQELEKAGLAERYLATPQ
HHEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCH
VIHGQRQKWVSYVSEREGLTKRQREILLVLRHEGGEMWLSELVGVAKTTATTVTKMAKVG
HHHCHHHHHHHHHHHHCCHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
CVAIAEREKLRLHQSPSQGVDPAKTLTNAQQAALETIKKIQGYGEVLLHGVTGSGKTEVY
HHEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHH
LQAIAPLLSQQKSALVLVPEIGLTPQLMDRFRSRFGDRVLTYHSGLSAGERYDTWRQMLR
HHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHC
PDSQIIIGTRSAVFAPLPNLGMIILDEEHDSSYKQDQPAPTYHARTVARWRSQQENCPLL
CCCEEEEEECCHHCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEE
LGSATPALDTWVEFQTAQNSNYYYISLPERVYARPLPPVEIVDMRHELRVGNRSLFSQSL
EECCCCHHHHHHEEEECCCCCEEEEECCHHHHCCCCCCHHHHHCHHHHHCCCHHHHHHHH
RQALERITQTHEQGILFIARRGHSTFVSCRSCGYVMECPHCDVSLSYHYVQEGSLPLLRC
HHHHHHHHHHHCCCEEEEEECCCCCEEEHHCCCCEEECCCCCEEEEEEEEECCCCCEEEE
HYCDHSQIQPKHCPSCNSPYFKFFGNGTQKVFQALQKEFPQLRCLRFDSDTTRRKGAHRD
ECCCCCCCCCCCCCCCCCCHHEECCCCHHHHHHHHHHHCCCEEEEEECCCHHHHCCCHHH
LLGQFARGEADILLGTQMLTKGLDVAQVTLVGVIAADGLLHQSDYRAAERTFQTLTQVAG
HHHHHHCCCCCEEECHHHHHCCCCHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHC
RAGRGDEPGQVIIQTYSPEHPVIQAVKTHDYLTFAEREISQRQELDYPPFGKLILLRFSG
CCCCCCCCCEEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECC
ENHDQVRYTAEAIAEKCFPLLEPEDELLGPVPANILRVARRYRWQVVLKFPQRKTEIPDL
CCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHEEEEEECCCCCCCCCCH
TFLREFCPRPVSLSINVDPLYID
HHHHHHCCCCEEEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]