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The map label for this gene is trmJ [H]

Identifier: 170076970

GI number: 170076970

Start: 359997

End: 360749

Strand: Direct

Name: trmJ [H]

Synonym: SYNPCC7002_A0342

Alternate gene names: 170076970

Gene position: 359997-360749 (Clockwise)

Preceding gene: 170076968

Following gene: 170076973

Centisome position: 11.97

GC content: 52.86

Gene sequence:

>753_bases
TTGGCGAGTTTGACGCATATTCGGATTGTGGTTGTAGAACCTGCAGGGGCCCTAAATCTTGGCTCCATTGCCCGGGTGAT
GAAAAATATGGGCCTGGGGCAATTGTTCTTAGTGAATCCCCATTGTAACCATTTAGGCCAAGAGGCTTTTTTAATGGCAG
TCCATGGGCGGGAATTTCTCGAACGGGCGGTGGTTGTTCCCGATTTGACAACGGCATTGACGGGTTGCAAAAAGGCGATC
GCCACCACTGGACTCCAGCGCAGTTTACCGATGCCCGTGGAAACCCCCAAGGCAGCTTTGCCCTGGTTTTTAGAAGACGA
TCAACCCAGTGCGCTAATTTTCGGGCGGGAAGACCACGGCTTGAGTAATAGCGAATTAAACCAAGCCCAAAGGCTGGTCT
GCATTCCCACGGGGGATGCATATACGTCCCTCAATTTGGCCCAGGCGGTCGCTGTTTGTAGCTACGAATTGCGCAGTTTG
GCCACCGAGCAACAGGGTTTTCCTCCCAAGGAAAAGGCCGCACCACTGCCTGATTTGGCCGATCTCAGTGAATTAGAGGG
CTACTATCAGCACCTAGAGCGGGTGTTGTTGCAGGTGGGATTTCTATATCCCCACACCAGCACCTCACGGATGGAAACGT
TTCGTAATTTGTACCACCGTGCCAGTCCCAGCAGTCGAGAAGTGGCGATGTTACGGGGAATTTTACGGCAGGTGGAATGG
GCGATCGCCAATGCGGCTGCACCAGAACAATAA

Upstream 100 bases:

>100_bases
TGAGCCGATGGAATCAGAATGAGATTTGGCGATCGCCAGGGTTTTTTTGGGGTCACGCCCACGGCACAATGGGGAGTTGC
ACTGCGATGGAGAAAATAGC

Downstream 100 bases:

>100_bases
TATTTAGATCTGGTTTATACCTTTATATAGATGGATAATCTTAGCGACCACCGCTTCAATGCTGAGATCATCGGTAATTA
ACTCCACCGCATCGGGGGCC

Product: rRNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MASLTHIRIVVVEPAGALNLGSIARVMKNMGLGQLFLVNPHCNHLGQEAFLMAVHGREFLERAVVVPDLTTALTGCKKAI
ATTGLQRSLPMPVETPKAALPWFLEDDQPSALIFGREDHGLSNSELNQAQRLVCIPTGDAYTSLNLAQAVAVCSYELRSL
ATEQQGFPPKEKAAPLPDLADLSELEGYYQHLERVLLQVGFLYPHTSTSRMETFRNLYHRASPSSREVAMLRGILRQVEW
AIANAAAPEQ

Sequences:

>Translated_250_residues
MASLTHIRIVVVEPAGALNLGSIARVMKNMGLGQLFLVNPHCNHLGQEAFLMAVHGREFLERAVVVPDLTTALTGCKKAI
ATTGLQRSLPMPVETPKAALPWFLEDDQPSALIFGREDHGLSNSELNQAQRLVCIPTGDAYTSLNLAQAVAVCSYELRSL
ATEQQGFPPKEKAAPLPDLADLSELEGYYQHLERVLLQVGFLYPHTSTSRMETFRNLYHRASPSSREVAMLRGILRQVEW
AIANAAAPEQ
>Mature_249_residues
ASLTHIRIVVVEPAGALNLGSIARVMKNMGLGQLFLVNPHCNHLGQEAFLMAVHGREFLERAVVVPDLTTALTGCKKAIA
TTGLQRSLPMPVETPKAALPWFLEDDQPSALIFGREDHGLSNSELNQAQRLVCIPTGDAYTSLNLAQAVAVCSYELRSLA
TEQQGFPPKEKAAPLPDLADLSELEGYYQHLERVLLQVGFLYPHTSTSRMETFRNLYHRASPSSREVAMLRGILRQVEWA
IANAAAPEQ

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=240, Percent_Identity=40.4166666666667, Blast_Score=152, Evalue=1e-38,
Organism=Escherichia coli, GI1790865, Length=159, Percent_Identity=38.3647798742138, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27439; Mature: 27308

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLTHIRIVVVEPAGALNLGSIARVMKNMGLGQLFLVNPHCNHLGQEAFLMAVHGREFL
CCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHCCHHHHHHHHHHHHH
ERAVVVPDLTTALTGCKKAIATTGLQRSLPMPVETPKAALPWFLEDDQPSALIFGREDHG
HHHHCCCCHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCEECCCCCCEEEEECCCCC
LSNSELNQAQRLVCIPTGDAYTSLNLAQAVAVCSYELRSLATEQQGFPPKEKAAPLPDLA
CCHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHH
DLSELEGYYQHLERVLLQVGFLYPHTSTSRMETFRNLYHRASPSSREVAMLRGILRQVEW
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AIANAAAPEQ
HHHCCCCCCC
>Mature Secondary Structure 
ASLTHIRIVVVEPAGALNLGSIARVMKNMGLGQLFLVNPHCNHLGQEAFLMAVHGREFL
CCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHCCHHHHHHHHHHHHH
ERAVVVPDLTTALTGCKKAIATTGLQRSLPMPVETPKAALPWFLEDDQPSALIFGREDHG
HHHHCCCCHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCCCEECCCCCCEEEEECCCCC
LSNSELNQAQRLVCIPTGDAYTSLNLAQAVAVCSYELRSLATEQQGFPPKEKAAPLPDLA
CCHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHH
DLSELEGYYQHLERVLLQVGFLYPHTSTSRMETFRNLYHRASPSSREVAMLRGILRQVEW
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AIANAAAPEQ
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA