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The map label for this gene is dusA [H]

Identifier: 170026071

GI number: 170026071

Start: 4250459

End: 4251496

Strand: Reverse

Name: dusA [H]

Synonym: YPK_3858

Alternate gene names: 170026071

Gene position: 4251496-4250459 (Counterclockwise)

Preceding gene: 170026073

Following gene: 170026070

Centisome position: 90.66

GC content: 51.93

Gene sequence:

>1038_bases
ATGCACGAAGCTCAGACTTTTTCCAGTACGCCGGCCACCAAGCCGCAATACCCCCTGCAACGCTTCTCCGTCGCGCCGAT
GCTCGATTGGACGGATCGTCATTGCCGTTATTTTCACCGTCTGCTCACTAAGCAAGCTCTGTTGTATACCGAAATGGTGA
CCACCGGCGCGATTATCCACGGTAAAGCCGATTATCTGGCTTATAGCGAGCAGGACCATCCGGTCGCTTTGCAATTAGGG
GGCAGCGATCCACAGGCATTGGCGCACTGTGCCAAGCTGGCTGAGCAACGCGGTTATAACGAAATCAATCTGAATGTGGG
TTGTCCGTCAGATCGAGTACAAAATGGCCGCTTTGGTGCTTGTTTGATGGGGGAGGCCGATCTGGTTGCCGATTGCATTA
AAGCTATGCGTGATGCGGTAGCTATTCCGGTGACGGTAAAAACCCGTATTGGGATTGACCAACTGGATAGCTATGAGTTT
CTATGCGAATTCGTGCAAACCGTCGCAGAACGTGGCGAATGCGAAATTTTCACTATTCATGCCCGTAAAGCCTGGCTCTC
AGGGCTAAGCCCGAAAGAGAATCGTGAAGTGCCGCCACTGGATTATGAGCGTGTTTATCAGCTTAAGTGTGATTTCCCTG
CGTTGACGATTGCCATTAATGGTGGGGTGAAAACATTGGCTGAAGCTAAAGAGCACCTCAAGCACCTTGATGGCGTGATG
ATGGGCCGTGAAGCCTATCAGAATCCGGGAATTTTGACGCAAGTAGACCGCGAGCTGTTTGATCCTCACGCGCCAGTGGT
TGATAGCGTTAAAGCCATTGAGGCGCTGTATCCTTATATTGAGCAGGAATTGTCTCAAGGGGCCTATTTGGGCCACATAA
CCCGCCATATTCTGGGTATTTTCCAAGGAATCCCCGGAGCGCGCCAATGGCGTCGGCATTTAAGCGAAAATGCCCATAAG
CCCGGAGCGGGGGTTTCTGTTGTGGAGGAGGCTTTAGCGCTGGTATCCCCTTCGTACTACGAATCCGTAGGGGGTTAG

Upstream 100 bases:

>100_bases
TTCTATGGTATAATCAACGGTTCCACCCCTTTGTATCGTTCGCTCAGTGAGCAACATCTGGGGGAAATTAATGCAGTAAT
AACTGATGAAATCACCGTAA

Downstream 100 bases:

>100_bases
CGGCGTTCGTTACTTGTCCTCTTCTACAAATAATTATTATACACATATGGGCTTCACCTCTGCGAGGCCGCTGTCAGCCG
TATTCAAATATGCTTTCGGC

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MHEAQTFSSTPATKPQYPLQRFSVAPMLDWTDRHCRYFHRLLTKQALLYTEMVTTGAIIHGKADYLAYSEQDHPVALQLG
GSDPQALAHCAKLAEQRGYNEINLNVGCPSDRVQNGRFGACLMGEADLVADCIKAMRDAVAIPVTVKTRIGIDQLDSYEF
LCEFVQTVAERGECEIFTIHARKAWLSGLSPKENREVPPLDYERVYQLKCDFPALTIAINGGVKTLAEAKEHLKHLDGVM
MGREAYQNPGILTQVDRELFDPHAPVVDSVKAIEALYPYIEQELSQGAYLGHITRHILGIFQGIPGARQWRRHLSENAHK
PGAGVSVVEEALALVSPSYYESVGG

Sequences:

>Translated_345_residues
MHEAQTFSSTPATKPQYPLQRFSVAPMLDWTDRHCRYFHRLLTKQALLYTEMVTTGAIIHGKADYLAYSEQDHPVALQLG
GSDPQALAHCAKLAEQRGYNEINLNVGCPSDRVQNGRFGACLMGEADLVADCIKAMRDAVAIPVTVKTRIGIDQLDSYEF
LCEFVQTVAERGECEIFTIHARKAWLSGLSPKENREVPPLDYERVYQLKCDFPALTIAINGGVKTLAEAKEHLKHLDGVM
MGREAYQNPGILTQVDRELFDPHAPVVDSVKAIEALYPYIEQELSQGAYLGHITRHILGIFQGIPGARQWRRHLSENAHK
PGAGVSVVEEALALVSPSYYESVGG
>Mature_345_residues
MHEAQTFSSTPATKPQYPLQRFSVAPMLDWTDRHCRYFHRLLTKQALLYTEMVTTGAIIHGKADYLAYSEQDHPVALQLG
GSDPQALAHCAKLAEQRGYNEINLNVGCPSDRVQNGRFGACLMGEADLVADCIKAMRDAVAIPVTVKTRIGIDQLDSYEF
LCEFVQTVAERGECEIFTIHARKAWLSGLSPKENREVPPLDYERVYQLKCDFPALTIAINGGVKTLAEAKEHLKHLDGVM
MGREAYQNPGILTQVDRELFDPHAPVVDSVKAIEALYPYIEQELSQGAYLGHITRHILGIFQGIPGARQWRRHLSENAHK
PGAGVSVVEEALALVSPSYYESVGG

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=296, Percent_Identity=23.9864864864865, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI31742496, Length=239, Percent_Identity=28.0334728033473, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI145693211, Length=316, Percent_Identity=77.8481012658228, Blast_Score=531, Evalue=1e-152,
Organism=Escherichia coli, GI1789660, Length=246, Percent_Identity=26.8292682926829, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1788462, Length=225, Percent_Identity=29.3333333333333, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17507177, Length=168, Percent_Identity=26.1904761904762, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=268, Percent_Identity=25, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24580595, Length=245, Percent_Identity=25.7142857142857, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19920448, Length=245, Percent_Identity=25.7142857142857, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 38303; Mature: 38303

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEAQTFSSTPATKPQYPLQRFSVAPMLDWTDRHCRYFHRLLTKQALLYTEMVTTGAIIH
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEE
GKADYLAYSEQDHPVALQLGGSDPQALAHCAKLAEQRGYNEINLNVGCPSDRVQNGRFGA
CCHHHEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCE
CLMGEADLVADCIKAMRDAVAIPVTVKTRIGIDQLDSYEFLCEFVQTVAERGECEIFTIH
EECCCHHHHHHHHHHHHHHEECCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
ARKAWLSGLSPKENREVPPLDYERVYQLKCDFPALTIAINGGVKTLAEAKEHLKHLDGVM
HHHHHHCCCCCCCCCCCCCCCHHHHEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
MGREAYQNPGILTQVDRELFDPHAPVVDSVKAIEALYPYIEQELSQGAYLGHITRHILGI
HHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
FQGIPGARQWRRHLSENAHKPGAGVSVVEEALALVSPSYYESVGG
HHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MHEAQTFSSTPATKPQYPLQRFSVAPMLDWTDRHCRYFHRLLTKQALLYTEMVTTGAIIH
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEE
GKADYLAYSEQDHPVALQLGGSDPQALAHCAKLAEQRGYNEINLNVGCPSDRVQNGRFGA
CCHHHEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCE
CLMGEADLVADCIKAMRDAVAIPVTVKTRIGIDQLDSYEFLCEFVQTVAERGECEIFTIH
EECCCHHHHHHHHHHHHHHEECCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
ARKAWLSGLSPKENREVPPLDYERVYQLKCDFPALTIAINGGVKTLAEAKEHLKHLDGVM
HHHHHHCCCCCCCCCCCCCCCHHHHEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
MGREAYQNPGILTQVDRELFDPHAPVVDSVKAIEALYPYIEQELSQGAYLGHITRHILGI
HHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
FQGIPGARQWRRHLSENAHKPGAGVSVVEEALALVSPSYYESVGG
HHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]