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The map label for this gene is insB [H]

Identifier: 170026051

GI number: 170026051

Start: 4229720

End: 4229995

Strand: Reverse

Name: insB [H]

Synonym: YPK_3838

Alternate gene names: 170026051

Gene position: 4229995-4229720 (Counterclockwise)

Preceding gene: 170026052

Following gene: 170026048

Centisome position: 90.2

GC content: 42.39

Gene sequence:

>276_bases
ATGATCACCCGCGATGACTGGGGTAGCTATGCTAGGCTCGTACCGCAAGATAGCCATCTGACTGGCAAAATCTTTACTCA
GCGCATTGAACGCAACAACCTGACTTTACGTACATACATCAAAAGGCTCGCGCGCAAAACTATCTGCTTCTCACGCTCTA
CTGAGCTACATGAAAAAGTTATCGAAACCTGCATTGAAAAATACATGTTCTACTCATTGGAGGTATCACCCAAAAAATCA
GACAAATTGAATCTTTTTAGTCGAATATGGCTTTAG

Upstream 100 bases:

>100_bases
TAGAGGTGATTTAATTTATGTACTGGCCTATACTTTCGGACCGTGCACGAATGAAACTTGTCGTGAATTACTGGTGCTGC
TTACGCCCTTCAATATCGGT

Downstream 100 bases:

>100_bases
AAATAACAAAACCCGCACATTGGCGGGTTCGTTGACATTAAAAGGAAACATTCACTTTAATGTCAATGCACGGTAAATTT
ATGTTTTTTTCTATCCGGGC

Product: IS1 transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 91; Mature: 91

Protein sequence:

>91_residues
MITRDDWGSYARLVPQDSHLTGKIFTQRIERNNLTLRTYIKRLARKTICFSRSTELHEKVIETCIEKYMFYSLEVSPKKS
DKLNLFSRIWL

Sequences:

>Translated_91_residues
MITRDDWGSYARLVPQDSHLTGKIFTQRIERNNLTLRTYIKRLARKTICFSRSTELHEKVIETCIEKYMFYSLEVSPKKS
DKLNLFSRIWL
>Mature_91_residues
MITRDDWGSYARLVPQDSHLTGKIFTQRIERNNLTLRTYIKRLARKTICFSRSTELHEKVIETCIEKYMFYSLEVSPKKS
DKLNLFSRIWL

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=57, Percent_Identity=49.1228070175439, Blast_Score=60, Evalue=3e-11,
Organism=Escherichia coli, GI1788203, Length=57, Percent_Identity=49.1228070175439, Blast_Score=60, Evalue=3e-11,
Organism=Escherichia coli, GI1786203, Length=57, Percent_Identity=49.1228070175439, Blast_Score=60, Evalue=3e-11,
Organism=Escherichia coli, GI1787223, Length=57, Percent_Identity=49.1228070175439, Blast_Score=59, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 10930; Mature: 10930

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITRDDWGSYARLVPQDSHLTGKIFTQRIERNNLTLRTYIKRLARKTICFSRSTELHEKV
CCCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
IETCIEKYMFYSLEVSPKKSDKLNLFSRIWL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MITRDDWGSYARLVPQDSHLTGKIFTQRIERNNLTLRTYIKRLARKTICFSRSTELHEKV
CCCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
IETCIEKYMFYSLEVSPKKSDKLNLFSRIWL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6265806 [H]