Click here to switch to the map view.

The map label for this gene is mepA [H]

Identifier: 170025934

GI number: 170025934

Start: 4109150

End: 4110211

Strand: Reverse

Name: mepA [H]

Synonym: YPK_3719

Alternate gene names: 170025934

Gene position: 4110211-4109150 (Counterclockwise)

Preceding gene: 170025936

Following gene: 170025933

Centisome position: 87.65

GC content: 52.92

Gene sequence:

>1062_bases
GTGGTGACCTTAAAAACTCAACCGGTCACATTGAGTAGCGATTTATCAGGCCGAACGGTGGCGGCAATGACGTCTGAAGT
CAGGCCACAGGTCGATGGTATCATCAAGAAACGCTTGTTTACTGAAGGATCTGAAGTCACCGCGGGACAAGTGCTCTATC
AAATAGACCCTGCTAGTTATCAGGCCGCTTATGACACCGCAAAAGCCGCGCTACAAAATGTTCAGGTCAGCGTAAAATCG
GCCAAGCTGAAAGCCCAGCGCTATGCGGCATTAGCCAAAGAAAATGGTGTGTCGCAGCAAGATGCTGATGACGCCCAAAC
CAGCTATCAGCAAGCGCTGGCCAATGTGGCCGAAAAAACTGCTGCGCTGGAAACGGCGCGTATCAATTTGGCCTATACCC
AAGTGCGGGCACCCATTTCAGGTCGCATCGGCATTTCCTCTGTGACACCAGGGGCGCTGGTGACGGCTAATCAAACCACG
GCATTAGCCACCATCCGTAACCTTGATCCCATTTATGTTGATTTAACCCAGTCCAGTGCGCAGTTGCTTGCTTTACGCAA
ACAGCAGCAGGCAGGCAATGACACAGTAGCGAATGCGCCGGTTCAGCTAACGCTGGAAGATGGCTCGGTTTATGCCCACG
AAGGTTCGCTGCAACTGACCGAGGTGGCCGTGGATGAAGCGACCGGTGCGGTGACCTTGCGTGCGAAATTCCCTAACCCT
GAACACCAATTATTGCCGGGCATGTTTGTTCGCGCTTCAGTACGGAACGGTGTAAATAACACCGCTATTTTGGCACCACA
GCAAGGCATCACTCATGATGCGAAAGGGAATGCTACCGCGTTGGTGGTTAATCAACAGCAGCAAGTTGAGCGGCGTGAAG
TGGTAACCGAACGCACGATTGATAGCTACTGGTTGATTAGCCGTGGGCTGGCTGCAGGTGACCGCCTGATTGTTGAAGGA
ACCGAAAAGGTCAGTGTTGGCGATGACGTCAAACCGGTAGAGGTGAGCACTACGCTGCCCGTTGTGGCCGAACCGACTAC
GCCATCAACTGGGGAGAAATAA

Upstream 100 bases:

>100_bases
TATGCACAGTAAAATTATTTTGGCCTGTTTGGTGTTCACGCTTGTCGCTTGTGATCAATCCTCTTCACCTTCAGCGACAC
CCTCGCGACAAGAGGTGGGG

Downstream 100 bases:

>100_bases
TCGATGTTTGCCCGTTTCTTTATTTATCGCCCGGTTTTTGCCTGGGTCATTGCCATTGTTATTATGTTGGGCGGGGTGGT
TGCGCTGGAAACCTTGCCGA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MVTLKTQPVTLSSDLSGRTVAAMTSEVRPQVDGIIKKRLFTEGSEVTAGQVLYQIDPASYQAAYDTAKAALQNVQVSVKS
AKLKAQRYAALAKENGVSQQDADDAQTSYQQALANVAEKTAALETARINLAYTQVRAPISGRIGISSVTPGALVTANQTT
ALATIRNLDPIYVDLTQSSAQLLALRKQQQAGNDTVANAPVQLTLEDGSVYAHEGSLQLTEVAVDEATGAVTLRAKFPNP
EHQLLPGMFVRASVRNGVNNTAILAPQQGITHDAKGNATALVVNQQQQVERREVVTERTIDSYWLISRGLAAGDRLIVEG
TEKVSVGDDVKPVEVSTTLPVVAEPTTPSTGEK

Sequences:

>Translated_353_residues
MVTLKTQPVTLSSDLSGRTVAAMTSEVRPQVDGIIKKRLFTEGSEVTAGQVLYQIDPASYQAAYDTAKAALQNVQVSVKS
AKLKAQRYAALAKENGVSQQDADDAQTSYQQALANVAEKTAALETARINLAYTQVRAPISGRIGISSVTPGALVTANQTT
ALATIRNLDPIYVDLTQSSAQLLALRKQQQAGNDTVANAPVQLTLEDGSVYAHEGSLQLTEVAVDEATGAVTLRAKFPNP
EHQLLPGMFVRASVRNGVNNTAILAPQQGITHDAKGNATALVVNQQQQVERREVVTERTIDSYWLISRGLAAGDRLIVEG
TEKVSVGDDVKPVEVSTTLPVVAEPTTPSTGEK
>Mature_353_residues
MVTLKTQPVTLSSDLSGRTVAAMTSEVRPQVDGIIKKRLFTEGSEVTAGQVLYQIDPASYQAAYDTAKAALQNVQVSVKS
AKLKAQRYAALAKENGVSQQDADDAQTSYQQALANVAEKTAALETARINLAYTQVRAPISGRIGISSVTPGALVTANQTT
ALATIRNLDPIYVDLTQSSAQLLALRKQQQAGNDTVANAPVQLTLEDGSVYAHEGSLQLTEVAVDEATGAVTLRAKFPNP
EHQLLPGMFVRASVRNGVNNTAILAPQQGITHDAKGNATALVVNQQQQVERREVVTERTIDSYWLISRGLAAGDRLIVEG
TEKVSVGDDVKPVEVSTTLPVVAEPTTPSTGEK

Specific function: The periplasmic linker protein component of an organic solvent and antibiotic efflux pump; confers resistance to toluene, hexane, p-xylene, ampicillin, penicillin G, erythromycin, novobiocin and tetracycline [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=330, Percent_Identity=52.4242424242424, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI1786668, Length=340, Percent_Identity=50.8823529411765, Blast_Score=313, Evalue=8e-87,
Organism=Escherichia coli, GI1789929, Length=329, Percent_Identity=46.8085106382979, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI87082036, Length=326, Percent_Identity=28.8343558282209, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI87081796, Length=320, Percent_Identity=24.6875, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 37516; Mature: 37516

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTLKTQPVTLSSDLSGRTVAAMTSEVRPQVDGIIKKRLFTEGSEVTAGQVLYQIDPASY
CEEEECCCEEECCCCCCCEEEEHHHHCCHHHHHHHHHHHHCCCCCEECCEEEEEECCCHH
QAAYDTAKAALQNVQVSVKSAKLKAQRYAALAKENGVSQQDADDAQTSYQQALANVAEKT
HHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
AALETARINLAYTQVRAPISGRIGISSVTPGALVTANQTTALATIRNLDPIYVDLTQSSA
HHHHHEEEEEEEEEEECCCCCCEEECCCCCCEEEECCCCEEEEECCCCCEEEEEECCCHH
QLLALRKQQQAGNDTVANAPVQLTLEDGSVYAHEGSLQLTEVAVDEATGAVTLRAKFPNP
HHHHHHHHHHCCCCCCCCCCEEEEEECCEEEEECCCEEEEEEEEECCCCEEEEEEECCCC
EHQLLPGMFVRASVRNGVNNTAILAPQQGITHDAKGNATALVVNQQQQVERREVVTERTI
CHHHCCCCEEEHHHHCCCCCCEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCC
DSYWLISRGLAAGDRLIVEGTEKVSVGDDVKPVEVSTTLPVVAEPTTPSTGEK
CHHHHHHCCCCCCCEEEEECCCEEECCCCCCCEEEECCCCEEECCCCCCCCCC
>Mature Secondary Structure
MVTLKTQPVTLSSDLSGRTVAAMTSEVRPQVDGIIKKRLFTEGSEVTAGQVLYQIDPASY
CEEEECCCEEECCCCCCCEEEEHHHHCCHHHHHHHHHHHHCCCCCEECCEEEEEECCCHH
QAAYDTAKAALQNVQVSVKSAKLKAQRYAALAKENGVSQQDADDAQTSYQQALANVAEKT
HHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
AALETARINLAYTQVRAPISGRIGISSVTPGALVTANQTTALATIRNLDPIYVDLTQSSA
HHHHHEEEEEEEEEEECCCCCCEEECCCCCCEEEECCCCEEEEECCCCCEEEEEECCCHH
QLLALRKQQQAGNDTVANAPVQLTLEDGSVYAHEGSLQLTEVAVDEATGAVTLRAKFPNP
HHHHHHHHHHCCCCCCCCCCEEEEEECCEEEEECCCEEEEEEEEECCCCEEEEEEECCCC
EHQLLPGMFVRASVRNGVNNTAILAPQQGITHDAKGNATALVVNQQQQVERREVVTERTI
CHHHCCCCEEEHHHHCCCCCCEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCC
DSYWLISRGLAAGDRLIVEGTEKVSVGDDVKPVEVSTTLPVVAEPTTPSTGEK
CHHHHHHCCCCCCCEEEEECCCEEECCCCCCCEEEECCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9827328 [H]