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The map label for this gene is dcuB [H]

Identifier: 170025574

GI number: 170025574

Start: 3685284

End: 3686627

Strand: Direct

Name: dcuB [H]

Synonym: YPK_3359

Alternate gene names: 170025574

Gene position: 3685284-3686627 (Clockwise)

Preceding gene: 170025573

Following gene: 170025575

Centisome position: 78.59

GC content: 45.83

Gene sequence:

>1344_bases
ATGATAACTCTACAATTTATAATTATAATTCTATGTTTGCTGTTAGGTACGCATTACGGAGGGATGGGGCTGGGGTTAAT
AAGTGGTATCGGCTTATTTATTCTGACCTTTATCTTTGGCCTCGAACCAGGAAAACCACCTGTAGATGTAATGCTAACAA
TATTAGCGGTTATTGGTTGTGCTTCAGTACTACAAACCGCAGGCGGGTTAAATGTAATGATGCAATTTGCCGAGCGATTA
TTACGCCGCCACCCACAGCACATTACGTTATTAGCGCCCTTCACGACGTGGTCATTAACCTTTCTTTGCGGCACCGGACA
TGTCGTTTACACCATGTTCCCCATAATTTCCGACATCGCCATTAAAAAAGGTATTCGTCCAGAACGACCAATGGCGGTTG
CATCCGTTGCCTCACAGATGGCAATTACGGCATCTCCAGTATCGGTTGCTGTCGTATCATTAGTTTCAATTATTGGTGCT
AACCATGGCATCGGCCATGCCTATAGCATTCTGGAAATTCTGGCTATTTCAGTTCCCGCCTCTTTGGTTGGAGTGCTAAT
CGCCGCCCTGTGGAGCCTGCGCCGTGGTAAAGATCTGGATAAAGATCCTGATTTTCAGAAAAAAATAAAAGATCCAGAGC
AGCGGGCCTATATATATGGCTCAACAGATACGTTGCTGAATCAGGTTTTTCCAAAACAAGCTTATTGGTCGACCTGGATA
TTCTTTGCCGCTATCGTCATTGTCGTCTTGTTGGGCGCTTTTGCAGACCTGCGCCCTGCTTTTGAAGTTAAAGGCAAGTT
ACAGTCTTTGTCGATGAATCTGGTGATTCAGATGATGATGCTGATCGCCGGTGCCGTTATTTTGATTGGCTGCAAAGTCA
AACCCAGTGATATCTCTAACGGTGCCGTATTCAAAGCCGGAATGGTTGCTATCTTCTCGGTATTCGGCGTTGCCTGGATG
AGTGACACCTTCTTCCAGGCTCATATGGGTGAACTGAGATTAGTGCTGGAAGATGTGGTAAAAGGCCACCCTTGGACTTA
TGCCATTGTGCTGTTCTTAGTCTCGAAGCTGGTGAACAGCCAAGCCGCGGCGCTAGCAGCGATCGCACCAATGGGCTTAC
AATTAGGTGTTGAACCTAAGATGCTCATCGCATTTTTCCCAGCAGCTTACGGATATTTTGTTCTGCCAACCTACCCGAGT
GATCTGGCTTGTATCGGTTTTGACCGCTCTGGTACCACCAAAATTGGTAGATTCATTATCAACCATAGCTTTATCATTCC
AGGTCTTATTGGCGTCATTTGCTCCTGTATCACGGGCTATCTACTGGTAACGACGTTTATGTAG

Upstream 100 bases:

>100_bases
TCTGTGCTCTGATAAGAGAAATCGAGTAAGGATTATAGTCATCGAATAAAACCAACAAATACATAACCGAATTTCATTCA
TATTTGAAGCGAGGGACATC

Downstream 100 bases:

>100_bases
TGAATCATAAATCCAAGGGTCTACTTTGTAGGGCCAGTGTATTTTCTGGCTCTTAATCCCCTTCTAATTTGACGCTGCCA
CAGTATTAATGGCATTCATT

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MITLQFIIIILCLLLGTHYGGMGLGLISGIGLFILTFIFGLEPGKPPVDVMLTILAVIGCASVLQTAGGLNVMMQFAERL
LRRHPQHITLLAPFTTWSLTFLCGTGHVVYTMFPIISDIAIKKGIRPERPMAVASVASQMAITASPVSVAVVSLVSIIGA
NHGIGHAYSILEILAISVPASLVGVLIAALWSLRRGKDLDKDPDFQKKIKDPEQRAYIYGSTDTLLNQVFPKQAYWSTWI
FFAAIVIVVLLGAFADLRPAFEVKGKLQSLSMNLVIQMMMLIAGAVILIGCKVKPSDISNGAVFKAGMVAIFSVFGVAWM
SDTFFQAHMGELRLVLEDVVKGHPWTYAIVLFLVSKLVNSQAAALAAIAPMGLQLGVEPKMLIAFFPAAYGYFVLPTYPS
DLACIGFDRSGTTKIGRFIINHSFIIPGLIGVICSCITGYLLVTTFM

Sequences:

>Translated_447_residues
MITLQFIIIILCLLLGTHYGGMGLGLISGIGLFILTFIFGLEPGKPPVDVMLTILAVIGCASVLQTAGGLNVMMQFAERL
LRRHPQHITLLAPFTTWSLTFLCGTGHVVYTMFPIISDIAIKKGIRPERPMAVASVASQMAITASPVSVAVVSLVSIIGA
NHGIGHAYSILEILAISVPASLVGVLIAALWSLRRGKDLDKDPDFQKKIKDPEQRAYIYGSTDTLLNQVFPKQAYWSTWI
FFAAIVIVVLLGAFADLRPAFEVKGKLQSLSMNLVIQMMMLIAGAVILIGCKVKPSDISNGAVFKAGMVAIFSVFGVAWM
SDTFFQAHMGELRLVLEDVVKGHPWTYAIVLFLVSKLVNSQAAALAAIAPMGLQLGVEPKMLIAFFPAAYGYFVLPTYPS
DLACIGFDRSGTTKIGRFIINHSFIIPGLIGVICSCITGYLLVTTFM
>Mature_447_residues
MITLQFIIIILCLLLGTHYGGMGLGLISGIGLFILTFIFGLEPGKPPVDVMLTILAVIGCASVLQTAGGLNVMMQFAERL
LRRHPQHITLLAPFTTWSLTFLCGTGHVVYTMFPIISDIAIKKGIRPERPMAVASVASQMAITASPVSVAVVSLVSIIGA
NHGIGHAYSILEILAISVPASLVGVLIAALWSLRRGKDLDKDPDFQKKIKDPEQRAYIYGSTDTLLNQVFPKQAYWSTWI
FFAAIVIVVLLGAFADLRPAFEVKGKLQSLSMNLVIQMMMLIAGAVILIGCKVKPSDISNGAVFKAGMVAIFSVFGVAWM
SDTFFQAHMGELRLVLEDVVKGHPWTYAIVLFLVSKLVNSQAAALAAIAPMGLQLGVEPKMLIAFFPAAYGYFVLPTYPS
DLACIGFDRSGTTKIGRFIINHSFIIPGLIGVICSCITGYLLVTTFM

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790565, Length=410, Percent_Identity=57.8048780487805, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI1790580, Length=444, Percent_Identity=37.1621621621622, Blast_Score=277, Evalue=1e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 48247; Mature: 48247

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLQFIIIILCLLLGTHYGGMGLGLISGIGLFILTFIFGLEPGKPPVDVMLTILAVIGC
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
ASVLQTAGGLNVMMQFAERLLRRHPQHITLLAPFTTWSLTFLCGTGHVVYTMFPIISDIA
HHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCHHHEEEEEECCCHHHHHHHHHHHHHH
IKKGIRPERPMAVASVASQMAITASPVSVAVVSLVSIIGANHGIGHAYSILEILAISVPA
HHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCH
SLVGVLIAALWSLRRGKDLDKDPDFQKKIKDPEQRAYIYGSTDTLLNQVFPKQAYWSTWI
HHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHCEEEECCHHHHHHHHCCCHHHHHHHH
FFAAIVIVVLLGAFADLRPAFEVKGKLQSLSMNLVIQMMMLIAGAVILIGCKVKPSDISN
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCC
GAVFKAGMVAIFSVFGVAWMSDTFFQAHMGELRLVLEDVVKGHPWTYAIVLFLVSKLVNS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
QAAALAAIAPMGLQLGVEPKMLIAFFPAAYGYFVLPTYPSDLACIGFDRSGTTKIGRFII
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCEEEEECCCCCCHHHHHHHH
NHSFIIPGLIGVICSCITGYLLVTTFM
CCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MITLQFIIIILCLLLGTHYGGMGLGLISGIGLFILTFIFGLEPGKPPVDVMLTILAVIGC
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
ASVLQTAGGLNVMMQFAERLLRRHPQHITLLAPFTTWSLTFLCGTGHVVYTMFPIISDIA
HHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCHHHEEEEEECCCHHHHHHHHHHHHHH
IKKGIRPERPMAVASVASQMAITASPVSVAVVSLVSIIGANHGIGHAYSILEILAISVPA
HHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCH
SLVGVLIAALWSLRRGKDLDKDPDFQKKIKDPEQRAYIYGSTDTLLNQVFPKQAYWSTWI
HHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCHHCEEEECCHHHHHHHHCCCHHHHHHHH
FFAAIVIVVLLGAFADLRPAFEVKGKLQSLSMNLVIQMMMLIAGAVILIGCKVKPSDISN
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCC
GAVFKAGMVAIFSVFGVAWMSDTFFQAHMGELRLVLEDVVKGHPWTYAIVLFLVSKLVNS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
QAAALAAIAPMGLQLGVEPKMLIAFFPAAYGYFVLPTYPSDLACIGFDRSGTTKIGRFII
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCEEEEECCCCCCHHHHHHHH
NHSFIIPGLIGVICSCITGYLLVTTFM
CCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]