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The map label for this gene is tolA [H]

Identifier: 170025179

GI number: 170025179

Start: 3255751

End: 3256932

Strand: Reverse

Name: tolA [H]

Synonym: YPK_2957

Alternate gene names: 170025179

Gene position: 3256932-3255751 (Counterclockwise)

Preceding gene: 170025180

Following gene: 170025178

Centisome position: 69.45

GC content: 50.85

Gene sequence:

>1182_bases
GTGTGGAAATCGAGCGTGGGTAAGGCAACCGAGCAAAATGATAAGCTGAATCGCGCCGTTATTGTTTCGGTGGTTCTGCA
CATCATATTGATTGCCCTGTTGATTTGGGGCTCTCTGACGCAGACGACTGAAATGGGCGGTGGCGGTGCTGGCGGCGAAG
TGATTGACGCTGTAATGGTCGATCCGGGTGCGGTGACAGAGCAATATAATCGTCAACAGCAACAGCAAACAGATGCTAAA
CGTGCCGAGCAGCAGCGTCAGAAAAAAGCTGAGCAGCAAGCCGAAGAATTGCAGCAAAAGCAGGCTGCTGAACAACAGCG
GCTGAAAGAATTAGAAAAAGAGCGTCTGCAAGCGCAAGAAGATGCGAAGCTGGCTGCTGAAGAGCAGAAAAAGCAAGTTG
CTGAACAACAAAAACAAATCGCTGAACAGCAAAAGCAGGCCGCTGAACAACAAAAAATTGCTGCTGCTGCCGTTGCAAAA
GCGAAAGAAGAGCAGAAGCAGGCTGAAACGGCTGCGGCTCAGGCAAAAGCTGAAGCAGATAAAATAGTGAAAGCGCAGGC
TGAAGCACAGAAAAAAGCCGAGGCTGAAGCCAAAAAAGAAGCTGCTGTTGCCGCTGCGGCGAAGAAACAAGCCGATGCCG
ATGCGAAGAAAGCGGTAGAGGTTGCCGAAAAGGCTGCGGCTGACGCAGCAGAAAAGAAAGCGGCCGCAGATGCAGAGAAA
AAAGCCGCTGCTGCTAAAAAAGTTGCTGCCGCCGCTGAGGCCAAGAAAAAAGCGGCAGCAGAAGCGGCTGCCTCAACGGA
TGTTGATGATTTGTTTGGTGGTTTGGCAAACGCTAAAAATGCACCGAAGAGTGGTTCGGGTGCAGGTGCCGCTGCTGCCG
GTAAAGGTGGCGGTAAGAAAAGCGGGGCATCAGGTGCTGATATCAGCGGCTACCTGGGGCAAATCACCGGCGCGATACAA
AGTAAATTTTATGATGCAGATCTTTACAAAGGCCGTACCTGTGATTTGCGAATCAAATTGGCACCAGACGGTCTATTAAT
CGATGTGAAAGCAGAAGGGGGGGACCCGGCACTGTGCCAGGCTGCTATCGCTGCGGCAAAACAAGCGAAAATTCCTAAGC
CTCCAAGTACGGATGTCTATGAACAATTTAAGAATGCTCCGCTTGTGTTTAAGCCTCAATAA

Upstream 100 bases:

>100_bases
ATAAATTTGCGTTGATATTGGTTGGGCTAATCGTTAGCTCAACCCGTAGATACCCTGTTTTTAGCAGTTCCGTTTTTTAG
CAATAGAGCGCGGACATCGA

Downstream 100 bases:

>100_bases
GGTTTGATTGACAGGGTGTAACGTTGTTAGTTAATGATGGATCATGTAGTTTTGTTTGCGGTGGTTTGTTAAAATTCTGC
TAATTATCGCGGGCATCCCG

Product: cell envelope integrity inner membrane protein TolA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MWKSSVGKATEQNDKLNRAVIVSVVLHIILIALLIWGSLTQTTEMGGGGAGGEVIDAVMVDPGAVTEQYNRQQQQQTDAK
RAEQQRQKKAEQQAEELQQKQAAEQQRLKELEKERLQAQEDAKLAAEEQKKQVAEQQKQIAEQQKQAAEQQKIAAAAVAK
AKEEQKQAETAAAQAKAEADKIVKAQAEAQKKAEAEAKKEAAVAAAAKKQADADAKKAVEVAEKAAADAAEKKAAADAEK
KAAAAKKVAAAAEAKKKAAAEAAASTDVDDLFGGLANAKNAPKSGSGAGAAAAGKGGGKKSGASGADISGYLGQITGAIQ
SKFYDADLYKGRTCDLRIKLAPDGLLIDVKAEGGDPALCQAAIAAAKQAKIPKPPSTDVYEQFKNAPLVFKPQ

Sequences:

>Translated_393_residues
MWKSSVGKATEQNDKLNRAVIVSVVLHIILIALLIWGSLTQTTEMGGGGAGGEVIDAVMVDPGAVTEQYNRQQQQQTDAK
RAEQQRQKKAEQQAEELQQKQAAEQQRLKELEKERLQAQEDAKLAAEEQKKQVAEQQKQIAEQQKQAAEQQKIAAAAVAK
AKEEQKQAETAAAQAKAEADKIVKAQAEAQKKAEAEAKKEAAVAAAAKKQADADAKKAVEVAEKAAADAAEKKAAADAEK
KAAAAKKVAAAAEAKKKAAAEAAASTDVDDLFGGLANAKNAPKSGSGAGAAAAGKGGGKKSGASGADISGYLGQITGAIQ
SKFYDADLYKGRTCDLRIKLAPDGLLIDVKAEGGDPALCQAAIAAAKQAKIPKPPSTDVYEQFKNAPLVFKPQ
>Mature_393_residues
MWKSSVGKATEQNDKLNRAVIVSVVLHIILIALLIWGSLTQTTEMGGGGAGGEVIDAVMVDPGAVTEQYNRQQQQQTDAK
RAEQQRQKKAEQQAEELQQKQAAEQQRLKELEKERLQAQEDAKLAAEEQKKQVAEQQKQIAEQQKQAAEQQKIAAAAVAK
AKEEQKQAETAAAQAKAEADKIVKAQAEAQKKAEAEAKKEAAVAAAAKKQADADAKKAVEVAEKAAADAAEKKAAADAEK
KAAAAKKVAAAAEAKKKAAAEAAASTDVDDLFGGLANAKNAPKSGSGAGAAAAGKGGGKKSGASGADISGYLGQITGAIQ
SKFYDADLYKGRTCDLRIKLAPDGLLIDVKAEGGDPALCQAAIAAAKQAKIPKPPSTDVYEQFKNAPLVFKPQ

Specific function: Involved in the tonB-independent uptake of group A colicins (colicins A, E1, E2, E3, and K). Necessary for the colicins to reach their respective targets after initial binding to the bacteria. Also involved in the translocation of bacteriophage DNA [H]

COG id: COG3064

COG function: function code M; Membrane protein involved in colicin uptake

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786960, Length=125, Percent_Identity=68.8, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014161 [H]

Pfam domain/function: PF06519 TolA [H]

EC number: NA

Molecular weight: Translated: 41012; Mature: 41012

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCCCCCCCCCHHHHHHCCCCCEECCC
>Mature Secondary Structure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HHHHHHHHCCCCCCCCCHHHHHHCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2687247; 8905232; 9278503; 2068069; 8978668; 10404600 [H]