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The map label for this gene is pbpG [H]

Identifier: 170025107

GI number: 170025107

Start: 3182192

End: 3183163

Strand: Reverse

Name: pbpG [H]

Synonym: YPK_2885

Alternate gene names: 170025107

Gene position: 3183163-3182192 (Counterclockwise)

Preceding gene: 170025109

Following gene: 170025104

Centisome position: 67.88

GC content: 45.47

Gene sequence:

>972_bases
ATGTCCACTCCCGATAATTTATTTAAGCTGACAAACATGTATGTAAAAATCCGTTTTGCACTATTGAGCTTTTTGCTTTT
ATCCACCGGTATCAGTGTTGCGCCTTTGGCCATTGCCCGGGGATCAGCGGTTGAGGTTAAAGGGACGGCACCTTTGGAAT
TGGCATCGGGTAGCGCTATGGTGGTTGATTTACAAACGAATAAAGTCATTTATGCCAATAATGCAGATAAAGTTGTGCCT
ATTGCCTCAATCACTAAACTGATGACGGCCATGGTGGTATTAGATGCCAAATTGCCTCTTGATGAAATTCTCTCCGTAGA
TATTGATCAAACTAAAGAGTTGAAAGGGGTGTTTTCCCGCGTTCGTGTTAACAGTGAAATTAGCCGTAAGGACATGCTGC
TACTGACACTGATGTCGTCAGAAAATCGTGCTGCGGCCAGCCTGGCCCACCATTACCCTGGGGGCTACAACGCATTTATA
AAAGCCATGAATGCAAAAGCCAAGTCTTTGGGCATGAGCAGTACGCATTATGTTGAGCCAACAGGGCTGTCGATTAATAA
CGTCTCGACAGCGCGTGATTTAGCCAAATTACTGATGGCGACCAAACAGTATCCGTTAATTGGCCAGTTAAGTACCACTA
CCGAAAAAATGGCTACTTTCCGTGAACCAAATTATACGTTGCCATTCCGCAATACTAACCATTTGGTTTATAACGACAAA
TGGAATATTCAACTGACCAAAACCGGTTTTACCAACCAGGCTGGACACTGCTTGGTCATGCGTACCGTGATTGGCAAGCG
GCCGGTCGCATTGGTGGTATTGGATGCTTTTGGTAAATATACCCATTTTGCGGATGCAAACCGCCTGCGTAGCTGGATCG
AAACAGGTAAAGCGGCGCCCATCCCTGGTGCTGCCAAAAGTTATCGTCAGCAAAAAGATTCACAGGGCCGTTTAGCGCAG
GTATCAGAATAA

Upstream 100 bases:

>100_bases
TGCTGCATAACAAACAGTCAACTTTAATCTTTGTAACAATCATTTAAAGAAACCGTGATGCGTGCGTCAGGCCGGTAATT
TATAATGCGCGACACCAATC

Downstream 100 bases:

>100_bases
GAGCAGCGCATCGCGACTGAGAAGCGGAATTTGCTAGCAGAGGCAAATTCCGCTTTAGTGACTAATTGTTTGCTTTCAGA
TAATGCCTAAGCCTAATGGT

Product: D-alanyl-D-alanine endopeptidase

Products: NA

Alternate protein names: DD-endopeptidase; Penicillin-binding protein 7; PBP-7 [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MSTPDNLFKLTNMYVKIRFALLSFLLLSTGISVAPLAIARGSAVEVKGTAPLELASGSAMVVDLQTNKVIYANNADKVVP
IASITKLMTAMVVLDAKLPLDEILSVDIDQTKELKGVFSRVRVNSEISRKDMLLLTLMSSENRAAASLAHHYPGGYNAFI
KAMNAKAKSLGMSSTHYVEPTGLSINNVSTARDLAKLLMATKQYPLIGQLSTTTEKMATFREPNYTLPFRNTNHLVYNDK
WNIQLTKTGFTNQAGHCLVMRTVIGKRPVALVVLDAFGKYTHFADANRLRSWIETGKAAPIPGAAKSYRQQKDSQGRLAQ
VSE

Sequences:

>Translated_323_residues
MSTPDNLFKLTNMYVKIRFALLSFLLLSTGISVAPLAIARGSAVEVKGTAPLELASGSAMVVDLQTNKVIYANNADKVVP
IASITKLMTAMVVLDAKLPLDEILSVDIDQTKELKGVFSRVRVNSEISRKDMLLLTLMSSENRAAASLAHHYPGGYNAFI
KAMNAKAKSLGMSSTHYVEPTGLSINNVSTARDLAKLLMATKQYPLIGQLSTTTEKMATFREPNYTLPFRNTNHLVYNDK
WNIQLTKTGFTNQAGHCLVMRTVIGKRPVALVVLDAFGKYTHFADANRLRSWIETGKAAPIPGAAKSYRQQKDSQGRLAQ
VSE
>Mature_322_residues
STPDNLFKLTNMYVKIRFALLSFLLLSTGISVAPLAIARGSAVEVKGTAPLELASGSAMVVDLQTNKVIYANNADKVVPI
ASITKLMTAMVVLDAKLPLDEILSVDIDQTKELKGVFSRVRVNSEISRKDMLLLTLMSSENRAAASLAHHYPGGYNAFIK
AMNAKAKSLGMSSTHYVEPTGLSINNVSTARDLAKLLMATKQYPLIGQLSTTTEKMATFREPNYTLPFRNTNHLVYNDKW
NIQLTKTGFTNQAGHCLVMRTVIGKRPVALVVLDAFGKYTHFADANRLRSWIETGKAAPIPGAAKSYRQQKDSQGRLAQV
SE

Specific function: Cell wall formation. May play a specialized role in remodeling the cell wall. Specifically hydrolyze the DD- diaminopimelate-alanine bonds in high-molecular-mass murein sacculi [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082054, Length=256, Percent_Identity=81.640625, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1787062, Length=285, Percent_Identity=30.1754385964912, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87082022, Length=255, Percent_Identity=28.2352941176471, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786851, Length=289, Percent_Identity=24.2214532871972, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR018044
- InterPro:   IPR001967 [H]

Pfam domain/function: PF00768 Peptidase_S11 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 35338; Mature: 35207

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPDNLFKLTNMYVKIRFALLSFLLLSTGISVAPLAIARGSAVEVKGTAPLELASGSAM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEECCCEEEECCCCCEEECCCCEE
VVDLQTNKVIYANNADKVVPIASITKLMTAMVVLDAKLPLDEILSVDIDQTKELKGVFSR
EEEEECCEEEEECCCCCEEHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHH
VRVNSEISRKDMLLLTLMSSENRAAASLAHHYPGGYNAFIKAMNAKAKSLGMSSTHYVEP
HHCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHCCHHHHCCCCCCCEECC
TGLSINNVSTARDLAKLLMATKQYPLIGQLSTTTEKMATFREPNYTLPFRNTNHLVYNDK
CCCEECCCHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHCCCCEEECCCCCCEEEECCC
WNIQLTKTGFTNQAGHCLVMRTVIGKRPVALVVLDAFGKYTHFADANRLRSWIETGKAAP
EEEEEEECCCCCCCCCEEEEEHHHCCCCEEEEEEEHHCCHHHHHHHHHHHHHHHCCCCCC
IPGAAKSYRQQKDSQGRLAQVSE
CCCHHHHHHHHCCCCCCEEECCC
>Mature Secondary Structure 
STPDNLFKLTNMYVKIRFALLSFLLLSTGISVAPLAIARGSAVEVKGTAPLELASGSAM
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEECCCEEEECCCCCEEECCCCEE
VVDLQTNKVIYANNADKVVPIASITKLMTAMVVLDAKLPLDEILSVDIDQTKELKGVFSR
EEEEECCEEEEECCCCCEEHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHH
VRVNSEISRKDMLLLTLMSSENRAAASLAHHYPGGYNAFIKAMNAKAKSLGMSSTHYVEP
HHCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCHHHHHHHHCCHHHHCCCCCCCEECC
TGLSINNVSTARDLAKLLMATKQYPLIGQLSTTTEKMATFREPNYTLPFRNTNHLVYNDK
CCCEECCCHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHCCCCEEECCCCCCEEEECCC
WNIQLTKTGFTNQAGHCLVMRTVIGKRPVALVVLDAFGKYTHFADANRLRSWIETGKAAP
EEEEEEECCCCCCCCCEEEEEHHHCCCCEEEEEEEHHCCHHHHHHHHHHHHHHHCCCCCC
IPGAAKSYRQQKDSQGRLAQVSE
CCCHHHHHHHHCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 6386470; 7721700; 7925376 [H]