Click here to switch to the map view.

The map label for this gene is nfo [H]

Identifier: 170024981

GI number: 170024981

Start: 3039982

End: 3040839

Strand: Direct

Name: nfo [H]

Synonym: YPK_2756

Alternate gene names: 170024981

Gene position: 3039982-3040839 (Clockwise)

Preceding gene: 170024980

Following gene: 170024990

Centisome position: 64.83

GC content: 49.3

Gene sequence:

>858_bases
ATGAAATTTGTCGGTGCACATGTCAGCGCAGCGGGTGGTGTAGATCAAGCGGTAATTAGAGCGCATGAACTTGAGGCCAC
GGCCTTTGCGCTGTTTACCAAGAATCAACGTCAATGGCGGGCCGCCCCTTTAGCGGAAGACGTAATTGAGAAATTTAAAC
TCGCGTGTGAGAAGTACGGCTATACCTCGGCACAAATTCTGCCTCACGATAGTTACCTGATTAATCTCGGGCACCCAGTC
ACCGAGGCACTGGAAAAATCCCGTGAAGCCTTTATCGATGAGTTAGTTCGCTGCCAGCAACTGGGGTTATCATTACTGAA
CTTCCATCCCGGTAGCCATTTACTGCAAATTGATGAAGACCAATGCTTGGCGCGGATTGCCGAATCGATCAACATCGCGT
TAGACGCCACTGAAGGCGTGACTGCAGTAATTGAAAATACCGCAGGTCAGGGCAGTAACCTGGGCTTTAAGTTTGAACAT
TTAGCCGCCATCATTGAAAGAGTGGAAGATAAAAGCCGCGTCGGCGTCTGTATTGATACCTGCCATGCTTTCGCCGCTGG
CTATGATTTACGGACTGAAGAAGATTGTGAGCACACCTTCGCGGCATTGGGCAAGATCGTCGGCTTCCAGTATCTGCGTG
GGATGCATCTTAATGATGCGAAAAGCGAATTTAACAGCCGGGTTGACCGCCACCACAGCCTGGGTGAAGGCAATATTGGC
AAAACCGTATTCAGCTATATTATGCGCGACTCACGTTTCGATAATATCCCATTGATTCTGGAAACGGTGAATATGGATAT
CTGGGCCGAAGAGATCGCCTGGCTGAAATCACAGGCAGAGATTGAGCCCTCGTTGTAA

Upstream 100 bases:

>100_bases
TAAGGGCCGTATCCGCGGCCCTTATTTCCCTTCACCGCTTATCCCATTCAATCATGTCCCTATCAATGCCATAATGGCCC
GGTTATCAAAGGAGAATGGA

Downstream 100 bases:

>100_bases
CGTAGAAAATGCCCGCCAGTAAACCTGGCGGGAATTGAACCTGACGGGAATTGAACCTAACGGGAATTGTGTCATCGCGG
ATGCCTTTAATGGCATACTC

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MKFVGAHVSAAGGVDQAVIRAHELEATAFALFTKNQRQWRAAPLAEDVIEKFKLACEKYGYTSAQILPHDSYLINLGHPV
TEALEKSREAFIDELVRCQQLGLSLLNFHPGSHLLQIDEDQCLARIAESINIALDATEGVTAVIENTAGQGSNLGFKFEH
LAAIIERVEDKSRVGVCIDTCHAFAAGYDLRTEEDCEHTFAALGKIVGFQYLRGMHLNDAKSEFNSRVDRHHSLGEGNIG
KTVFSYIMRDSRFDNIPLILETVNMDIWAEEIAWLKSQAEIEPSL

Sequences:

>Translated_285_residues
MKFVGAHVSAAGGVDQAVIRAHELEATAFALFTKNQRQWRAAPLAEDVIEKFKLACEKYGYTSAQILPHDSYLINLGHPV
TEALEKSREAFIDELVRCQQLGLSLLNFHPGSHLLQIDEDQCLARIAESINIALDATEGVTAVIENTAGQGSNLGFKFEH
LAAIIERVEDKSRVGVCIDTCHAFAAGYDLRTEEDCEHTFAALGKIVGFQYLRGMHLNDAKSEFNSRVDRHHSLGEGNIG
KTVFSYIMRDSRFDNIPLILETVNMDIWAEEIAWLKSQAEIEPSL
>Mature_285_residues
MKFVGAHVSAAGGVDQAVIRAHELEATAFALFTKNQRQWRAAPLAEDVIEKFKLACEKYGYTSAQILPHDSYLINLGHPV
TEALEKSREAFIDELVRCQQLGLSLLNFHPGSHLLQIDEDQCLARIAESINIALDATEGVTAVIENTAGQGSNLGFKFEH
LAAIIERVEDKSRVGVCIDTCHAFAAGYDLRTEEDCEHTFAALGKIVGFQYLRGMHLNDAKSEFNSRVDRHHSLGEGNIG
KTVFSYIMRDSRFDNIPLILETVNMDIWAEEIAWLKSQAEIEPSL

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=278, Percent_Identity=81.294964028777, Blast_Score=478, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17531193, Length=261, Percent_Identity=46.360153256705, Blast_Score=273, Evalue=7e-74,
Organism=Saccharomyces cerevisiae, GI6322735, Length=284, Percent_Identity=41.5492957746479, Blast_Score=229, Evalue=4e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 31672; Mature: 31672

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVGAHVSAAGGVDQAVIRAHELEATAFALFTKNQRQWRAAPLAEDVIEKFKLACEKYG
CCEECCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCHHHHCCCHHHHHHHHHHHHHHHCC
YTSAQILPHDSYLINLGHPVTEALEKSREAFIDELVRCQQLGLSLLNFHPGSHLLQIDED
CCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCEEEECHH
QCLARIAESINIALDATEGVTAVIENTAGQGSNLGFKFEHLAAIIERVEDKSRVGVCIDT
HHHHHHHHHHEEEEECCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHH
CHAFAAGYDLRTEEDCEHTFAALGKIVGFQYLRGMHLNDAKSEFNSRVDRHHSLGEGNIG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHH
KTVFSYIMRDSRFDNIPLILETVNMDIWAEEIAWLKSQAEIEPSL
HHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKFVGAHVSAAGGVDQAVIRAHELEATAFALFTKNQRQWRAAPLAEDVIEKFKLACEKYG
CCEECCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCHHHHCCCHHHHHHHHHHHHHHHCC
YTSAQILPHDSYLINLGHPVTEALEKSREAFIDELVRCQQLGLSLLNFHPGSHLLQIDED
CCCEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCEEEECHH
QCLARIAESINIALDATEGVTAVIENTAGQGSNLGFKFEHLAAIIERVEDKSRVGVCIDT
HHHHHHHHHHEEEEECCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHH
CHAFAAGYDLRTEEDCEHTFAALGKIVGFQYLRGMHLNDAKSEFNSRVDRHHSLGEGNIG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHH
KTVFSYIMRDSRFDNIPLILETVNMDIWAEEIAWLKSQAEIEPSL
HHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA