Click here to switch to the map view.

The map label for this gene is poxB [H]

Identifier: 170024928

GI number: 170024928

Start: 2986028

End: 2987749

Strand: Direct

Name: poxB [H]

Synonym: YPK_2703

Alternate gene names: 170024928

Gene position: 2986028-2987749 (Clockwise)

Preceding gene: 170024927

Following gene: 170024929

Centisome position: 63.68

GC content: 52.21

Gene sequence:

>1722_bases
ATGAAGCAAACTGTGGCAACACTGATCGCTAAAACGTTGGAACAGGCAGGCGTTAAGCGGATTTGGGGCGTAACCGGGGA
TTCACTCAATGGTCTGAGTGATAGCCTGCATCGCATGGGAACTATCGAGTGGCTGGGCACACGTCACGAAGAAGTGGCCG
CTTTCGCCGCCGGGGCGGAAGCACAGCTCACCGGGCAGCTAGCTGTATGCGCGGGTTCCTGTGGCCCCGGTAACCTGCAT
TTGATTAATGGCTTATTTGATTGTCACCGTAACCATGTCCCGGTACTCGCCATTGCTGCGCATATTCCCTCCAGTGAGAT
TGGTAGCGGTTATTTTCAGGAAACTCACCCACAGGAGTTATTCCGCGAATGTAGCCACTACTGTGAACTCGTTTCGAACC
CAGAACAGTTACCAAGAGTGTTAGAAATCGCCATGCGCCAAGCCATTCTTAATCGTGGCGTATCGGTTATTGTTCTGCCG
GGCGATGTTGCGTTACAGGCAGCGCCAGAAGATGCCGCCGTCATCTGGCAAACACCTAAATTACCATTAGTTCAGCCACC
GATGAGTGAGTTAACGCTCTTGGCTGACACCCTGAATAAAGCTAAAAATATTACGTTGATGTGCGGTAGCGGCTGCGCTG
ATGCTCATGATGAAGTGGTCAAATTGGCTGAGAGACTACAAGCCCCGGTGGTTCACGCCCTGCGTGGAAAAGAGCATATT
GAGTGGGACAACCCTTACAGTGTCGGTATGACCGGGCTGATTGGTTTCTCCTCAGGCTATCAAGCGATGCTCAATGCCGA
TACCCTGATTCTGCTGGGGACGCAGTTCCCTTACCGCGCGTTTTATCCCACTCACGCCAATATCATCCAGATTGATATTA
ATCCCGGAAGTCTTGGCGCTCATTGCCCCGTCAACATGGCGCTGGTTGGCGATATCAAAACCACCCTGACCGCGCTGTTG
CCGCAGTTAGATGCCAAAAGTGACCGCGCGTTTCTGGATAAAGCGCTGGAACATTACCGTACCACCCGCCAAGATCTGGA
TGCTCTCGCCACCGCGAATGATAACCAACCGATCCACCCACAATATCTGGCACAACAAATCAGTCACTACGCCACAGAAG
ATGCGATTTTCACCTGCGATGTGGGTACCCCAACCGTTTGGGCCGCACGTTATTTGCAGATGAACGGCAAGCGCCGTTTA
CTGGGGTCGTTTAACCATGGCTCAATGGCTAACGCCATGCCGCAAGCAATTGGTGCCCAAGCAACCGCCCCTGACCGACA
AGTCGTGGCCTTGTGCGGCGACGGTGGTTTCACCATGCTAATGGGGGATTTCCTCTCACTGACCCAGCTAAAATTACCCG
TAAAAATCGTGGTATTTAATAACAGCGTACTGGGGTTCGTGGCAATGGAGATGAAAGCGGGCGGCTATCTGACGGATGGT
ACGGACCTGCATAATCCAGACTTCGCCGCCATTGCCAACGCTGCTGGCATCAAAGGGATTCGGGTAGAAAAAGCCTCTGA
GTTAAATGCGGCACTGGAAAGTGCCTTTGCACATCCTGGCCCCGTATTGGTGGATGTCGTGACGGCGAAACAAGAGCTCT
CAATGCCACCACAGATTAAGTTTGAGCAAGCCAAAGGATTTAGTCTTTATATGCTCAGGGCCATTATTAATGGCCGGGGG
GATGAAGTGATCGAGCTGGCAAAAACTAACTGGTTACGCTAA

Upstream 100 bases:

>100_bases
AGTGCCTATCCGCATCATCAATGGGGCATTTATCCTCAAACACGCCATACTTTGACCTCATAGAATCATAACTTCGCTAA
TAGACCTTGAGGTAACAACT

Downstream 100 bases:

>100_bases
TCAAAAGGTCTTTCAATCTAGAGAGGTCTTGTAGCCAATAAAGATCTTTGTCATTTATCCAGCCAGTCGTATCGAATATG
TACCGACACGACTGGCGACA

Product: pyruvate dehydrogenase

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MKQTVATLIAKTLEQAGVKRIWGVTGDSLNGLSDSLHRMGTIEWLGTRHEEVAAFAAGAEAQLTGQLAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQELFRECSHYCELVSNPEQLPRVLEIAMRQAILNRGVSVIVLP
GDVALQAAPEDAAVIWQTPKLPLVQPPMSELTLLADTLNKAKNITLMCGSGCADAHDEVVKLAERLQAPVVHALRGKEHI
EWDNPYSVGMTGLIGFSSGYQAMLNADTLILLGTQFPYRAFYPTHANIIQIDINPGSLGAHCPVNMALVGDIKTTLTALL
PQLDAKSDRAFLDKALEHYRTTRQDLDALATANDNQPIHPQYLAQQISHYATEDAIFTCDVGTPTVWAARYLQMNGKRRL
LGSFNHGSMANAMPQAIGAQATAPDRQVVALCGDGGFTMLMGDFLSLTQLKLPVKIVVFNNSVLGFVAMEMKAGGYLTDG
TDLHNPDFAAIANAAGIKGIRVEKASELNAALESAFAHPGPVLVDVVTAKQELSMPPQIKFEQAKGFSLYMLRAIINGRG
DEVIELAKTNWLR

Sequences:

>Translated_573_residues
MKQTVATLIAKTLEQAGVKRIWGVTGDSLNGLSDSLHRMGTIEWLGTRHEEVAAFAAGAEAQLTGQLAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQELFRECSHYCELVSNPEQLPRVLEIAMRQAILNRGVSVIVLP
GDVALQAAPEDAAVIWQTPKLPLVQPPMSELTLLADTLNKAKNITLMCGSGCADAHDEVVKLAERLQAPVVHALRGKEHI
EWDNPYSVGMTGLIGFSSGYQAMLNADTLILLGTQFPYRAFYPTHANIIQIDINPGSLGAHCPVNMALVGDIKTTLTALL
PQLDAKSDRAFLDKALEHYRTTRQDLDALATANDNQPIHPQYLAQQISHYATEDAIFTCDVGTPTVWAARYLQMNGKRRL
LGSFNHGSMANAMPQAIGAQATAPDRQVVALCGDGGFTMLMGDFLSLTQLKLPVKIVVFNNSVLGFVAMEMKAGGYLTDG
TDLHNPDFAAIANAAGIKGIRVEKASELNAALESAFAHPGPVLVDVVTAKQELSMPPQIKFEQAKGFSLYMLRAIINGRG
DEVIELAKTNWLR
>Mature_573_residues
MKQTVATLIAKTLEQAGVKRIWGVTGDSLNGLSDSLHRMGTIEWLGTRHEEVAAFAAGAEAQLTGQLAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQELFRECSHYCELVSNPEQLPRVLEIAMRQAILNRGVSVIVLP
GDVALQAAPEDAAVIWQTPKLPLVQPPMSELTLLADTLNKAKNITLMCGSGCADAHDEVVKLAERLQAPVVHALRGKEHI
EWDNPYSVGMTGLIGFSSGYQAMLNADTLILLGTQFPYRAFYPTHANIIQIDINPGSLGAHCPVNMALVGDIKTTLTALL
PQLDAKSDRAFLDKALEHYRTTRQDLDALATANDNQPIHPQYLAQQISHYATEDAIFTCDVGTPTVWAARYLQMNGKRRL
LGSFNHGSMANAMPQAIGAQATAPDRQVVALCGDGGFTMLMGDFLSLTQLKLPVKIVVFNNSVLGFVAMEMKAGGYLTDG
TDLHNPDFAAIANAAGIKGIRVEKASELNAALESAFAHPGPVLVDVVTAKQELSMPPQIKFEQAKGFSLYMLRAIINGRG
DEVIELAKTNWLR

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=557, Percent_Identity=25.4937163375224, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1787096, Length=573, Percent_Identity=79.0575916230366, Blast_Score=928, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=554, Percent_Identity=28.158844765343, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1790104, Length=549, Percent_Identity=28.9617486338798, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI1786717, Length=549, Percent_Identity=26.0473588342441, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1788716, Length=555, Percent_Identity=25.045045045045, Blast_Score=106, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17531299, Length=569, Percent_Identity=26.713532513181, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17531301, Length=569, Percent_Identity=26.713532513181, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=572, Percent_Identity=26.048951048951, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6320816, Length=543, Percent_Identity=24.1252302025783, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922626, Length=540, Percent_Identity=26.6666666666667, Blast_Score=143, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 61832; Mature: 61832

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTVATLIAKTLEQAGVKRIWGVTGDSLNGLSDSLHRMGTIEWLGTRHEEVAAFAAGAE
CCHHHHHHHHHHHHHCCHHHECCCCCCCCCCHHHHHHHCCCHHHHCCCHHHHHHHHCCCC
AQLTGQLAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQEL
CEEECEEEEEECCCCCCCEEEEHHHHHHCCCCCCEEEEEECCCCHHCCCCCCCCCCHHHH
FRECSHYCELVSNPEQLPRVLEIAMRQAILNRGVSVIVLPGDVALQAAPEDAAVIWQTPK
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCEEEEECCC
LPLVQPPMSELTLLADTLNKAKNITLMCGSGCADAHDEVVKLAERLQAPVVHALRGKEHI
CCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
EWDNPYSVGMTGLIGFSSGYQAMLNADTLILLGTQFPYRAFYPTHANIIQIDINPGSLGA
CCCCCCCCCCEEEEECCCCCHHHCCCCEEEEEECCCCCEEECCCCCCEEEEEECCCCCCC
HCPVNMALVGDIKTTLTALLPQLDAKSDRAFLDKALEHYRTTRQDLDALATANDNQPIHP
CCCCCEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
QYLAQQISHYATEDAIFTCDVGTPTVWAARYLQMNGKRRLLGSFNHGSMANAMPQAIGAQ
HHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCC
ATAPDRQVVALCGDGGFTMLMGDFLSLTQLKLPVKIVVFNNSVLGFVAMEMKAGGYLTDG
CCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEEECCCCEEECC
TDLHNPDFAAIANAAGIKGIRVEKASELNAALESAFAHPGPVLVDVVTAKQELSMPPQIK
CCCCCCCHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCCEEEEEEECHHHCCCCCCEE
FEQAKGFSLYMLRAIINGRGDEVIELAKTNWLR
ECCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKQTVATLIAKTLEQAGVKRIWGVTGDSLNGLSDSLHRMGTIEWLGTRHEEVAAFAAGAE
CCHHHHHHHHHHHHHCCHHHECCCCCCCCCCHHHHHHHCCCHHHHCCCHHHHHHHHCCCC
AQLTGQLAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQEL
CEEECEEEEEECCCCCCCEEEEHHHHHHCCCCCCEEEEEECCCCHHCCCCCCCCCCHHHH
FRECSHYCELVSNPEQLPRVLEIAMRQAILNRGVSVIVLPGDVALQAAPEDAAVIWQTPK
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCEEEEECCC
LPLVQPPMSELTLLADTLNKAKNITLMCGSGCADAHDEVVKLAERLQAPVVHALRGKEHI
CCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
EWDNPYSVGMTGLIGFSSGYQAMLNADTLILLGTQFPYRAFYPTHANIIQIDINPGSLGA
CCCCCCCCCCEEEEECCCCCHHHCCCCEEEEEECCCCCEEECCCCCCEEEEEECCCCCCC
HCPVNMALVGDIKTTLTALLPQLDAKSDRAFLDKALEHYRTTRQDLDALATANDNQPIHP
CCCCCEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
QYLAQQISHYATEDAIFTCDVGTPTVWAARYLQMNGKRRLLGSFNHGSMANAMPQAIGAQ
HHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCC
ATAPDRQVVALCGDGGFTMLMGDFLSLTQLKLPVKIVVFNNSVLGFVAMEMKAGGYLTDG
CCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEEECCCCEEECC
TDLHNPDFAAIANAAGIKGIRVEKASELNAALESAFAHPGPVLVDVVTAKQELSMPPQIK
CCCCCCCHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCCEEEEEEECHHHCCCCCCEE
FEQAKGFSLYMLRAIINGRGDEVIELAKTNWLR
ECCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]