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The map label for this gene is pykA [H]

Identifier: 170024370

GI number: 170024370

Start: 2367143

End: 2368585

Strand: Direct

Name: pykA [H]

Synonym: YPK_2136

Alternate gene names: 170024370

Gene position: 2367143-2368585 (Clockwise)

Preceding gene: 170024369

Following gene: 170024371

Centisome position: 50.48

GC content: 50.24

Gene sequence:

>1443_bases
ATGTCCAGACGGCTCAGAAGGACCAAGATTGTTACTACACTGGGGCCGGCTACTGACCGCGACAATAATCTGGAAAAGAT
TATTGCTGCAGGTGCGAACGTAGTCCGCCTGAATTTTTCCCATGGTAGCGCTGAAGATCATGAATTACGGGCCAATAAAG
TCCGTGAAATCGCTGCCAGACTGGGGCGTCATGTAGCTATTCTTGGTGATTTGCAAGGTCCTAAAATCCGGGTATCGACG
TTTAAAGAAGGTAAAGTTTTCCTTAATATAAACGATAAATTCCTGCTGGATGCCAATATGGCAAAAGGCGAAGGTGATAA
AAACAAAGTGGGTATCGATTACAAAGGCCTGCCAGCTGATGTGGTTCCCGGTGATATTTTATTGCTTGATGATGGCCGCG
TGCAGTTAAAAGTGGTTGAAGTTCAGGGCATGAAAGTCTTCACCGAAGTGACCGTCGGTGGCCCGCTCTCTAACAATAAA
GGCATCAACAAGTTAGGTGGCGGGTTATCAGCCGAAGCCCTAACCGAAAAAGATAAAGCCGATATTGTTACCGCTGCAAA
AATTGGTGTGGACTTCCTGGCGGTCTCTTTCCCACGTACTGGTGAAGATTTGCATTATGCACGCCGCCTGGCCCGTGATG
CTGGCTGCAATGCACAGATCGTCGCAAAGGTTGAGCGCGCTGAAGCGGTCGCCACTGATGAGGCGATGGATGATATCATT
CTGGCCTCTGACGTCGTCATGGTCGCTCGTGGTGATTTGGGTGTTGAAATTGGCGACCCAGAACTTGTTGGTATCCAAAA
GAAACTGATCCGCCGTGCCCGCCAGCTAAACCGTGCCGTGATCACGGCGACTCAGATGATGGAGTCAATGATCACCAACC
CGATGCCAACCCGAGCAGAGGTGATGGATGTTGCCAACGCAGTGCTGGATGGTACCGATGCGGTCATGTTGTCAGCAGAA
ACAGCAGCAGGCCAGTACCCGGCAGAAACCGTAGCGGCAATGGCGCGTGTCTGTCTAGGCGCGGAAAAAATCCCAAGCAT
CAATGTGTCAAAACACCGTCTAGATGTGCAATTCGATAACGTCGAAGAAGCTATCGCGATGTCGACCATGTATGCGGCAA
ACCACCTCAAAGGTATCACCGCGATTATCGCCATGACTGAATCAGGCCGCACGGCTTTGATGATGTCGCGTATCAGTTCC
GGTTTACCTATTTTCGCCATGTCGCGCCATGATCATACTTTAAACCTAACCGCCATCTATCGTGGCGTGACGCCGGTCTA
TTGTGATCCCCATACTGACGGTGTTCTTGCCGCCACAGAAGCAGTAAAACGCTTGCAAGATAAAGGGTTCTTGGTGTCAG
GTGATTTGGTTATCGTTACTCAGGGCGATGTGATGGGCACCATTGGTACGACCAATACCAGTCGTATCATCCGCGTCGAA
TAA

Upstream 100 bases:

>100_bases
CGATTTTATCGTAAAATTTGTAGATTAATGACATTTTACCATCGGATCCATTATCCGCTGTTTTAACAACACCATGCTTC
ACCAGTCAACGGAGTAAGAG

Downstream 100 bases:

>100_bases
GTTATTTTTAAGATAGGCAGAAAAGCAAAGGCCCGCTATCACTAGCGGGCCTCAGACTGCTGACAAACCCCGATGACTCG
ATCTTGAACGGTGAGGATAG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MSRRLRRTKIVTTLGPATDRDNNLEKIIAAGANVVRLNFSHGSAEDHELRANKVREIAARLGRHVAILGDLQGPKIRVST
FKEGKVFLNINDKFLLDANMAKGEGDKNKVGIDYKGLPADVVPGDILLLDDGRVQLKVVEVQGMKVFTEVTVGGPLSNNK
GINKLGGGLSAEALTEKDKADIVTAAKIGVDFLAVSFPRTGEDLHYARRLARDAGCNAQIVAKVERAEAVATDEAMDDII
LASDVVMVARGDLGVEIGDPELVGIQKKLIRRARQLNRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAE
TAAGQYPAETVAAMARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSTMYAANHLKGITAIIAMTESGRTALMMSRISS
GLPIFAMSRHDHTLNLTAIYRGVTPVYCDPHTDGVLAATEAVKRLQDKGFLVSGDLVIVTQGDVMGTIGTTNTSRIIRVE

Sequences:

>Translated_480_residues
MSRRLRRTKIVTTLGPATDRDNNLEKIIAAGANVVRLNFSHGSAEDHELRANKVREIAARLGRHVAILGDLQGPKIRVST
FKEGKVFLNINDKFLLDANMAKGEGDKNKVGIDYKGLPADVVPGDILLLDDGRVQLKVVEVQGMKVFTEVTVGGPLSNNK
GINKLGGGLSAEALTEKDKADIVTAAKIGVDFLAVSFPRTGEDLHYARRLARDAGCNAQIVAKVERAEAVATDEAMDDII
LASDVVMVARGDLGVEIGDPELVGIQKKLIRRARQLNRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAE
TAAGQYPAETVAAMARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSTMYAANHLKGITAIIAMTESGRTALMMSRISS
GLPIFAMSRHDHTLNLTAIYRGVTPVYCDPHTDGVLAATEAVKRLQDKGFLVSGDLVIVTQGDVMGTIGTTNTSRIIRVE
>Mature_479_residues
SRRLRRTKIVTTLGPATDRDNNLEKIIAAGANVVRLNFSHGSAEDHELRANKVREIAARLGRHVAILGDLQGPKIRVSTF
KEGKVFLNINDKFLLDANMAKGEGDKNKVGIDYKGLPADVVPGDILLLDDGRVQLKVVEVQGMKVFTEVTVGGPLSNNKG
INKLGGGLSAEALTEKDKADIVTAAKIGVDFLAVSFPRTGEDLHYARRLARDAGCNAQIVAKVERAEAVATDEAMDDIIL
ASDVVMVARGDLGVEIGDPELVGIQKKLIRRARQLNRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAET
AAGQYPAETVAAMARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSTMYAANHLKGITAIIAMTESGRTALMMSRISSG
LPIFAMSRHDHTLNLTAIYRGVTPVYCDPHTDGVLAATEAVKRLQDKGFLVSGDLVIVTQGDVMGTIGTTNTSRIIRVE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=502, Percent_Identity=37.4501992031873, Blast_Score=264, Evalue=2e-70,
Organism=Homo sapiens, GI33286420, Length=502, Percent_Identity=37.4501992031873, Blast_Score=264, Evalue=2e-70,
Organism=Homo sapiens, GI33286418, Length=495, Percent_Identity=37.3737373737374, Blast_Score=258, Evalue=9e-69,
Organism=Homo sapiens, GI10835121, Length=496, Percent_Identity=35.8870967741936, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI32967597, Length=496, Percent_Identity=35.8870967741936, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=35.5172413793103, Blast_Score=149, Evalue=8e-36,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=35.5172413793103, Blast_Score=149, Evalue=8e-36,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=33.8983050847458, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=33.8983050847458, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=33.4883720930233, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=89.5833333333333, Blast_Score=874, Evalue=0.0,
Organism=Escherichia coli, GI1787965, Length=490, Percent_Identity=36.3265306122449, Blast_Score=282, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI17544584, Length=493, Percent_Identity=36.105476673428, Blast_Score=260, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI17506829, Length=495, Percent_Identity=36.969696969697, Blast_Score=238, Evalue=5e-63,
Organism=Caenorhabditis elegans, GI71984413, Length=495, Percent_Identity=36.969696969697, Blast_Score=238, Evalue=5e-63,
Organism=Caenorhabditis elegans, GI71984406, Length=495, Percent_Identity=36.969696969697, Blast_Score=238, Evalue=6e-63,
Organism=Caenorhabditis elegans, GI17506831, Length=495, Percent_Identity=36.969696969697, Blast_Score=238, Evalue=6e-63,
Organism=Saccharomyces cerevisiae, GI6324923, Length=496, Percent_Identity=34.4758064516129, Blast_Score=249, Evalue=8e-67,
Organism=Saccharomyces cerevisiae, GI6319279, Length=489, Percent_Identity=35.5828220858896, Blast_Score=246, Evalue=7e-66,
Organism=Drosophila melanogaster, GI24648964, Length=501, Percent_Identity=36.3273453093812, Blast_Score=263, Evalue=3e-70,
Organism=Drosophila melanogaster, GI28571814, Length=501, Percent_Identity=36.3273453093812, Blast_Score=263, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24648966, Length=455, Percent_Identity=33.4065934065934, Blast_Score=226, Evalue=4e-59,
Organism=Drosophila melanogaster, GI24581235, Length=494, Percent_Identity=31.7813765182186, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24646914, Length=270, Percent_Identity=35.1851851851852, Blast_Score=145, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51544; Mature: 51413

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRLRRTKIVTTLGPATDRDNNLEKIIAAGANVVRLNFSHGSAEDHELRANKVREIAAR
CCCCCCHHEEEEEECCCCCCCCCHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHH
LGRHVAILGDLQGPKIRVSTFKEGKVFLNINDKFLLDANMAKGEGDKNKVGIDYKGLPAD
HCCEEEEEECCCCCEEEEEEEECCEEEEEECCEEEEECCCCCCCCCCCEECCEECCCCCC
VVPGDILLLDDGRVQLKVVEVQGMKVFTEVTVGGPLSNNKGINKLGGGLSAEALTEKDKA
CCCCCEEEEECCCEEEEEEEECCEEEEEEEEECCCCCCCCCCHHHCCCCCHHHHCCCCHH
DIVTAAKIGVDFLAVSFPRTGEDLHYARRLARDAGCNAQIVAKVERAEAVATDEAMDDII
CEEEEHHHCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHH
LASDVVMVARGDLGVEIGDPELVGIQKKLIRRARQLNRAVITATQMMESMITNPMPTRAE
HHCCEEEEEECCCCEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VMDVANAVLDGTDAVMLSAETAAGQYPAETVAAMARVCLGAEKIPSINVSKHRLDVQFDN
HHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCHHHCCCCCCCCEEEEEEECC
VEEAIAMSTMYAANHLKGITAIIAMTESGRTALMMSRISSGLPIFAMSRHDHTLNLTAIY
HHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHCCCCCEEEEECCCCEEEEEEEE
RGVTPVYCDPHTDGVLAATEAVKRLQDKGFLVSGDLVIVTQGDVMGTIGTTNTSRIIRVE
CCCCCEEECCCCCCCHHHHHHHHHHHHCCEEEECCEEEEECCCEEEEECCCCCCEEEEEC
>Mature Secondary Structure 
SRRLRRTKIVTTLGPATDRDNNLEKIIAAGANVVRLNFSHGSAEDHELRANKVREIAAR
CCCCCHHEEEEEECCCCCCCCCHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHH
LGRHVAILGDLQGPKIRVSTFKEGKVFLNINDKFLLDANMAKGEGDKNKVGIDYKGLPAD
HCCEEEEEECCCCCEEEEEEEECCEEEEEECCEEEEECCCCCCCCCCCEECCEECCCCCC
VVPGDILLLDDGRVQLKVVEVQGMKVFTEVTVGGPLSNNKGINKLGGGLSAEALTEKDKA
CCCCCEEEEECCCEEEEEEEECCEEEEEEEEECCCCCCCCCCHHHCCCCCHHHHCCCCHH
DIVTAAKIGVDFLAVSFPRTGEDLHYARRLARDAGCNAQIVAKVERAEAVATDEAMDDII
CEEEEHHHCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHH
LASDVVMVARGDLGVEIGDPELVGIQKKLIRRARQLNRAVITATQMMESMITNPMPTRAE
HHCCEEEEEECCCCEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VMDVANAVLDGTDAVMLSAETAAGQYPAETVAAMARVCLGAEKIPSINVSKHRLDVQFDN
HHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCHHHCCCCCCCCEEEEEEECC
VEEAIAMSTMYAANHLKGITAIIAMTESGRTALMMSRISSGLPIFAMSRHDHTLNLTAIY
HHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHCCCCCEEEEECCCCEEEEEEEE
RGVTPVYCDPHTDGVLAATEAVKRLQDKGFLVSGDLVIVTQGDVMGTIGTTNTSRIIRVE
CCCCCEEECCCCCCCHHHHHHHHHHHHCCEEEECCEEEEECCCEEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]