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The map label for this gene is thiD [H]

Identifier: 170023563

GI number: 170023563

Start: 1450495

End: 1451295

Strand: Direct

Name: thiD [H]

Synonym: YPK_1321

Alternate gene names: 170023563

Gene position: 1450495-1451295 (Clockwise)

Preceding gene: 170023562

Following gene: 170023565

Centisome position: 30.93

GC content: 55.93

Gene sequence:

>801_bases
ATGAAGCGTATTAATACACTGACGATTGCCGGTACTGACCCGAGTGGTGGGGCGGGTATTCAGGCTGATCTGAAAACATT
CTCTGCTTTACGGGCCTATGGTACCAGTGTCATTACCGCGCTGGTGGCACAAAACACCCGTGGTGTGCAGTCGGTTTATC
CTATCGATCCTCATTTTGTTGCCGCTCAGTTGGATTCCGTTCTCAGTGACGTGCGTATCGACAGCGCCAAAATTGGTATG
CTGGCGAATACTGCGGTGGTCGAAATGGTCGCCGAGCAGTTGCGCCGTTATCCTATTCCGTTCGTGGTTTTGGATACGGT
GATGGTGGCCAAAAGTGGAGATCCGCTATTGGCCCCTGACGCCGTGGAGGCCGTTCGTCAGTGGCTGTTGCCGCAAGTCG
CATTAATTACGCCGAATTTGCCAGAGGCCGCCGCGTTACTGGGGTGTCCACCGGCCTGCGATGAACAACAAATGCGTGAG
CAGGGACGGGCGCTATTAGCCTTAGGCTGTCAGGGGGTGTTAATGAAAGGCGGGCACTTAACCGGCGAGGATAGCCCAGA
CTGGTTGTTTACCGCAGAGGGGGAGCAGCGTTTTGTGGCTCAACGCGTGGCTACCCGGAATACTCATGGTACGGGGTGTA
CGCTATCGGCGGCATTGGCAGCACTGCGCCCGCGATACGGCCATTGGGGGGAAACGGTGGCGGTAGCTAAACACTATTTG
CAATGTGCGCTGGAACAGGCCGATACCCTGGAGGTGGGGCAGGGCGTTGGGCCTGTTCATCATTTTCATCAATGGTGGTA
A

Upstream 100 bases:

>100_bases
TAGGAGGGAATATGTACGTTCTACCTGCCGTTCTCTTTTCAGCCACTGCGGCTGAATTTACGCCATTAGCCCCCTCAATG
ATGAGTACGGAGACGTATTC

Downstream 100 bases:

>100_bases
TGAAAATGATGAAAATTGCAGGTGGGTTTATTGATTGGTACGCTCTGGCGTCGCCGTGCTGTCGCCAGTGGCTGGTCCCG
ACAAATATTGCTGTGCCAGA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MKRINTLTIAGTDPSGGAGIQADLKTFSALRAYGTSVITALVAQNTRGVQSVYPIDPHFVAAQLDSVLSDVRIDSAKIGM
LANTAVVEMVAEQLRRYPIPFVVLDTVMVAKSGDPLLAPDAVEAVRQWLLPQVALITPNLPEAAALLGCPPACDEQQMRE
QGRALLALGCQGVLMKGGHLTGEDSPDWLFTAEGEQRFVAQRVATRNTHGTGCTLSAALAALRPRYGHWGETVAVAKHYL
QCALEQADTLEVGQGVGPVHHFHQWW

Sequences:

>Translated_266_residues
MKRINTLTIAGTDPSGGAGIQADLKTFSALRAYGTSVITALVAQNTRGVQSVYPIDPHFVAAQLDSVLSDVRIDSAKIGM
LANTAVVEMVAEQLRRYPIPFVVLDTVMVAKSGDPLLAPDAVEAVRQWLLPQVALITPNLPEAAALLGCPPACDEQQMRE
QGRALLALGCQGVLMKGGHLTGEDSPDWLFTAEGEQRFVAQRVATRNTHGTGCTLSAALAALRPRYGHWGETVAVAKHYL
QCALEQADTLEVGQGVGPVHHFHQWW
>Mature_266_residues
MKRINTLTIAGTDPSGGAGIQADLKTFSALRAYGTSVITALVAQNTRGVQSVYPIDPHFVAAQLDSVLSDVRIDSAKIGM
LANTAVVEMVAEQLRRYPIPFVVLDTVMVAKSGDPLLAPDAVEAVRQWLLPQVALITPNLPEAAALLGCPPACDEQQMRE
QGRALLALGCQGVLMKGGHLTGEDSPDWLFTAEGEQRFVAQRVATRNTHGTGCTLSAALAALRPRYGHWGETVAVAKHYL
QCALEQADTLEVGQGVGPVHHFHQWW

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=266, Percent_Identity=76.6917293233083, Blast_Score=409, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6325378, Length=270, Percent_Identity=32.2222222222222, Blast_Score=121, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324997, Length=271, Percent_Identity=36.1623616236162, Blast_Score=116, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6324517, Length=271, Percent_Identity=36.1623616236162, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28524; Mature: 28524

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRINTLTIAGTDPSGGAGIQADLKTFSALRAYGTSVITALVAQNTRGVQSVYPIDPHFV
CCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHH
AAQLDSVLSDVRIDSAKIGMLANTAVVEMVAEQLRRYPIPFVVLDTVMVAKSGDPLLAPD
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCH
AVEAVRQWLLPQVALITPNLPEAAALLGCPPACDEQQMREQGRALLALGCQGVLMKGGHL
HHHHHHHHHCCCEEEECCCCCHHHHHHCCCCCCCHHHHHHHCCEEEEECCCEEEEECCCC
TGEDSPDWLFTAEGEQRFVAQRVATRNTHGTGCTLSAALAALRPRYGHWGETVAVAKHYL
CCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHH
QCALEQADTLEVGQGVGPVHHFHQWW
HHHHHHCCHHHHCCCCCHHHHHHCCC
>Mature Secondary Structure
MKRINTLTIAGTDPSGGAGIQADLKTFSALRAYGTSVITALVAQNTRGVQSVYPIDPHFV
CCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHH
AAQLDSVLSDVRIDSAKIGMLANTAVVEMVAEQLRRYPIPFVVLDTVMVAKSGDPLLAPD
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCH
AVEAVRQWLLPQVALITPNLPEAAALLGCPPACDEQQMREQGRALLALGCQGVLMKGGHL
HHHHHHHHHCCCEEEECCCCCHHHHHHCCCCCCCHHHHHHHCCEEEEECCCEEEEECCCC
TGEDSPDWLFTAEGEQRFVAQRVATRNTHGTGCTLSAALAALRPRYGHWGETVAVAKHYL
CCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHH
QCALEQADTLEVGQGVGPVHHFHQWW
HHHHHHCCHHHHCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]