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The map label for this gene is rlmN [H]

Identifier: 170023536

GI number: 170023536

Start: 1419271

End: 1420467

Strand: Direct

Name: rlmN [H]

Synonym: YPK_1290

Alternate gene names: 170023536

Gene position: 1419271-1420467 (Clockwise)

Preceding gene: 170023535

Following gene: 170023537

Centisome position: 30.27

GC content: 48.45

Gene sequence:

>1197_bases
ATGTCTGAACAATTATTAACTGCATCAACGTCAATTGATGCCGCACCTCTGTCTGACAATACCGTTCAGACCACCGCACC
TGCTACCAGCAAAATCAATCTGCTCGATTTAAATCGTCAGCAAATGCGCGAGTTTTTTGCCGAAATGGGAGAAAAACCTT
TCCGGGCAGATCAGGTCATGAAGTGGATGTATCACTATTGCTACGACGATTTTGAGCAAATGACCGACATTAATAAAGGG
CTGCGGGCGAAATTGCAGCGTGTGGCAGAGATCCGTGCGCCGGAAGTTGCAGAAGAGCAACGCTCTGTCGATGGCACCAT
TAAATGGGCAATTAAAGTCGGCGATCAACAAGTTGAAACGGTATATATCCCTGAAGCAGACCGCGCCACGCTGTGTGTGT
CATCCCAAGTCGGTTGTGCATTGGAGTGTAAATTCTGTTCGACCGCACAGCAGGGCTTTAACCGTAATCTTCGCGTATCT
GAAATTATCGGTCAGGTATGGCGTGCGGCAAAAATTATTGGTTCATTGAAATCAACCGGTACCCGGCCCATAACCAACGT
GGTCATGATGGGGATGGGGGAACCACTACTGAATCTGAATAATGTCGTACCAGCGATGGATATCATGATGGACGACTTCG
GTTTTGGTTTATCCAAACGCCGTGTGACGCTGTCCACCTCTGGGGTGGTTCCGGCGTTGGATAAACTGGGCGATATGATT
GACGTTGCGTTGGCTATCTCGTTACATGCGCCTACCGATGATATTCGCGATGAAATCGTCCCTATCAACCGTAAATATAA
TATTGAAACGTTCCTGGCTGCAGTGCGCCGTTATTTGGATAAATCCAAAGCCAACGGCGGGCGGGTTACCGTTGAGTACG
TTATGCTGGATCATATCAATGACAGCACTGAGCAAGCCCATCAGTTAGCTGAGTGTTTGAAAGATACGCCCTGCAAGATC
AATTTGATCCCATGGAACCCGTTCCCTGGTGCACCTTATGGCCGTAGCTCAAACAGCCGGGTAGATCGCTTTTCTAAGGT
GTTGATGGAATACGGCTTTACCACTATCGTGCGTAAGACCCGTGGCGATGACATTGATGCCGCATGTGGCCAATTGGCCG
GTGAGGTTATCGACCGTACTAAACGCACCTTGAAAAAGAAAATGGCGGGTGAACCTATTGCGATTAAGACAGTCTGA

Upstream 100 bases:

>100_bases
TTCTATTATTTATACATTCTATTATTTATCGCCGTTTCGGAAGCCGTACCCGGTTTAAAGCGCAGCGCCGTAAAGTGTAA
CAACGAGGCCACGACAACTC

Downstream 100 bases:

>100_bases
TTCATAAGATTGATTCACCAGATAACTAATTGTTATTTAACTTATAATTTGTTGAAGTCTCATCGCGTAAAGGTGGCTAA
AAACCCGTGATGAGAATACC

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSEQLLTASTSIDAAPLSDNTVQTTAPATSKINLLDLNRQQMREFFAEMGEKPFRADQVMKWMYHYCYDDFEQMTDINKG
LRAKLQRVAEIRAPEVAEEQRSVDGTIKWAIKVGDQQVETVYIPEADRATLCVSSQVGCALECKFCSTAQQGFNRNLRVS
EIIGQVWRAAKIIGSLKSTGTRPITNVVMMGMGEPLLNLNNVVPAMDIMMDDFGFGLSKRRVTLSTSGVVPALDKLGDMI
DVALAISLHAPTDDIRDEIVPINRKYNIETFLAAVRRYLDKSKANGGRVTVEYVMLDHINDSTEQAHQLAECLKDTPCKI
NLIPWNPFPGAPYGRSSNSRVDRFSKVLMEYGFTTIVRKTRGDDIDAACGQLAGEVIDRTKRTLKKKMAGEPIAIKTV

Sequences:

>Translated_398_residues
MSEQLLTASTSIDAAPLSDNTVQTTAPATSKINLLDLNRQQMREFFAEMGEKPFRADQVMKWMYHYCYDDFEQMTDINKG
LRAKLQRVAEIRAPEVAEEQRSVDGTIKWAIKVGDQQVETVYIPEADRATLCVSSQVGCALECKFCSTAQQGFNRNLRVS
EIIGQVWRAAKIIGSLKSTGTRPITNVVMMGMGEPLLNLNNVVPAMDIMMDDFGFGLSKRRVTLSTSGVVPALDKLGDMI
DVALAISLHAPTDDIRDEIVPINRKYNIETFLAAVRRYLDKSKANGGRVTVEYVMLDHINDSTEQAHQLAECLKDTPCKI
NLIPWNPFPGAPYGRSSNSRVDRFSKVLMEYGFTTIVRKTRGDDIDAACGQLAGEVIDRTKRTLKKKMAGEPIAIKTV
>Mature_397_residues
SEQLLTASTSIDAAPLSDNTVQTTAPATSKINLLDLNRQQMREFFAEMGEKPFRADQVMKWMYHYCYDDFEQMTDINKGL
RAKLQRVAEIRAPEVAEEQRSVDGTIKWAIKVGDQQVETVYIPEADRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSE
IIGQVWRAAKIIGSLKSTGTRPITNVVMMGMGEPLLNLNNVVPAMDIMMDDFGFGLSKRRVTLSTSGVVPALDKLGDMID
VALAISLHAPTDDIRDEIVPINRKYNIETFLAAVRRYLDKSKANGGRVTVEYVMLDHINDSTEQAHQLAECLKDTPCKIN
LIPWNPFPGAPYGRSSNSRVDRFSKVLMEYGFTTIVRKTRGDDIDAACGQLAGEVIDRTKRTLKKKMAGEPIAIKTV

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=398, Percent_Identity=83.1658291457286, Blast_Score=693, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 44295; Mature: 44164

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQLLTASTSIDAAPLSDNTVQTTAPATSKINLLDLNRQQMREFFAEMGEKPFRADQVM
CCCCEEECCCCCCCCCCCCCCEEECCCCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHHH
KWMYHYCYDDFEQMTDINKGLRAKLQRVAEIRAPEVAEEQRSVDGTIKWAIKVGDQQVET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEEEECCCEEEE
VYIPEADRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIIGSLKSTG
EECCCCCCCEEEECCCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
TRPITNVVMMGMGEPLLNLNNVVPAMDIMMDDFGFGLSKRRVTLSTSGVVPALDKLGDMI
CCHHHHHHHHCCCCCHHCHHCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHH
DVALAISLHAPTDDIRDEIVPINRKYNIETFLAAVRRYLDKSKANGGRVTVEYVMLDHIN
HHHHEEEECCCCHHHHHCEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCC
DSTEQAHQLAECLKDTPCKINLIPWNPFPGAPYGRSSNSRVDRFSKVLMEYGFTTIVRKT
CHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHC
RGDDIDAACGQLAGEVIDRTKRTLKKKMAGEPIAIKTV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure 
SEQLLTASTSIDAAPLSDNTVQTTAPATSKINLLDLNRQQMREFFAEMGEKPFRADQVM
CCCEEECCCCCCCCCCCCCCEEECCCCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHHH
KWMYHYCYDDFEQMTDINKGLRAKLQRVAEIRAPEVAEEQRSVDGTIKWAIKVGDQQVET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEEEECCCEEEE
VYIPEADRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIIGSLKSTG
EECCCCCCCEEEECCCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
TRPITNVVMMGMGEPLLNLNNVVPAMDIMMDDFGFGLSKRRVTLSTSGVVPALDKLGDMI
CCHHHHHHHHCCCCCHHCHHCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHH
DVALAISLHAPTDDIRDEIVPINRKYNIETFLAAVRRYLDKSKANGGRVTVEYVMLDHIN
HHHHEEEECCCCHHHHHCEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCC
DSTEQAHQLAECLKDTPCKINLIPWNPFPGAPYGRSSNSRVDRFSKVLMEYGFTTIVRKT
CHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHC
RGDDIDAACGQLAGEVIDRTKRTLKKKMAGEPIAIKTV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA