| Definition | Yersinia pseudotuberculosis YPIII chromosome, complete genome. |
|---|---|
| Accession | NC_010465 |
| Length | 4,689,441 |
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The map label for this gene is nadE [H]
Identifier: 170023507
GI number: 170023507
Start: 1388819
End: 1390441
Strand: Direct
Name: nadE [H]
Synonym: YPK_1261
Alternate gene names: 170023507
Gene position: 1388819-1390441 (Clockwise)
Preceding gene: 170023506
Following gene: 170023508
Centisome position: 29.62
GC content: 49.91
Gene sequence:
>1623_bases ATGAGCAGATCACTTTCCATTGCGTTAGCCCAGTTGAATTGGCTGGTCGGCGATATTGAAGGCAACACTGAACGTATGTT GCAAACCCTAAACGAGCAGCAGAAGGCGGGTGCCGATCTGGTCATGTTCTCCGAGCTGGCTTTGTCCGGTTATCCACCGG AAGATCTTCTGTACCGTGATGACTTTTATCAACGTTGTGAAGTGCAACTTGAGCGTTTGCAAGCGGCAACCCCGCATATT GCCGTGCTGGTGGGCCACCCATGGCGGGAAGGGGATAAGCTGTATAATGCACTGTCGCTGTTTGCACAAGGTAAACTGCT GGGGCGCTATTTTAAACAACAACTGCCTAATTACGGCGTATTTGATGAAAAACGCTATTTCTCCGCAGGTCATCAAAGCT GTGTCGTTGAGCTGAAAGGCTACCGCCTGGGTCTACTGATTTGTGAAGACTTATGGCTTGATGGTCCGATAGATGCAGTG AAAGCCGCGGGCGCAGAAGTGGTATTGTCGATCAATGCCTCGCCTTATAATCGTGAAAAACCCTATATTCGTAAAACGCT GATGGCCGCTCATTGCCAGCGTACGGGCTTACCACTGGTGTATCTCAACCAGGTTGGTGGGCAGGATGAGCTGATTTTTG ATGGCTGCTCCAAAGTCTTCGATGCGGCGGGTAACATGACCCATCGTTTGGCTGCATTTGCAGAGCAAACCACGCTGTTG CAGTTAAATGAGTGTAACGTGATCCCGATGACGGCACCTGTGGCGGAACTGCCTCCTCTGGCTCAAGTCTATGAAGCCTT GGTATTGGCGGTACGTGACTACGTCACCAAGAATGGCTTTAATGGGGCAGTGCTGGGTTTATCTGGCGGCATTGACTCAG CATTGACATTGGCCATTGCGGTTGATGCGTTGGGCAAAGACAAGGTTCAGGCATTGATGATGCCATTCCGCTATACCGCA GATATCAGTATTGCTGATGCCAAAGAAGAAGCTGAAATACTGGGTGTCAAATTTGATGTGATGTCTATCGAACCCATGTT TGATGCTTTTATGGGCCAGTTGGCACCGATGTTTGCCGATACCGGGCGCGATACCACGGAGGAGAACCTTCAGGCTCGTT GCCGTGGCGTGGTTCTGATGGCTTTATCGAATAAACGCCGCAGTATTGTATTAACTACGGGTAACAAAAGTGAAATGGCC GTGGGCTACGCGACGCTGTACGGTGATATGGCGGGGGGCTTTGATGTCCTGAAAGATGTACCGAAGACGTTAGTATTCAA ATTAGCGGAATACCGTAATACGGTCTCTTATGTGATCCCGCAGCGCGTGATCACGCGTCCGCCTTCAGCCGAATTGGCAC CTGATCAGAAAGATGAAGATAGCCTGCCTCCTTATGATATTTTGGACGCTATCCTTGAAGGATATGTTGAACAGGATAAG TCAGTGGCTGAGCTGGTGGCTGACGGTTTTGATGAAGCTATCGTGCGTAAAGTCATCCGCCTGGTCGACATTAATGAATA TAAACGCCGCCAGTCAGCTGTAGGGCCACGTATTACCGCCCGCAGTTTTGGTAAAGATAGACGCTATCCGATCACATCAG GCTTTGGCCGCAAGAATTGGTAA
Upstream 100 bases:
>100_bases GTAAAGGCGGGGCCCGTGATGGGCCAGCCAATGCCCCTGTGGTTTTGAAAGATGAGGGTATATTATTCCCTGACAATGCT GAAACAGAGTTTAAACACCA
Downstream 100 bases:
>100_bases AACAGGGATTCACCATGAAAAAAATTGACGCGATTATTAAACCATTCAAACTGGATGACGTACGTGAAGCGCTGGCTGAA GTCGGTATTACCGGGATGAC
Product: NAD synthetase
Products: NA
Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]
Number of amino acids: Translated: 540; Mature: 539
Protein sequence:
>540_residues MSRSLSIALAQLNWLVGDIEGNTERMLQTLNEQQKAGADLVMFSELALSGYPPEDLLYRDDFYQRCEVQLERLQAATPHI AVLVGHPWREGDKLYNALSLFAQGKLLGRYFKQQLPNYGVFDEKRYFSAGHQSCVVELKGYRLGLLICEDLWLDGPIDAV KAAGAEVVLSINASPYNREKPYIRKTLMAAHCQRTGLPLVYLNQVGGQDELIFDGCSKVFDAAGNMTHRLAAFAEQTTLL QLNECNVIPMTAPVAELPPLAQVYEALVLAVRDYVTKNGFNGAVLGLSGGIDSALTLAIAVDALGKDKVQALMMPFRYTA DISIADAKEEAEILGVKFDVMSIEPMFDAFMGQLAPMFADTGRDTTEENLQARCRGVVLMALSNKRRSIVLTTGNKSEMA VGYATLYGDMAGGFDVLKDVPKTLVFKLAEYRNTVSYVIPQRVITRPPSAELAPDQKDEDSLPPYDILDAILEGYVEQDK SVAELVADGFDEAIVRKVIRLVDINEYKRRQSAVGPRITARSFGKDRRYPITSGFGRKNW
Sequences:
>Translated_540_residues MSRSLSIALAQLNWLVGDIEGNTERMLQTLNEQQKAGADLVMFSELALSGYPPEDLLYRDDFYQRCEVQLERLQAATPHI AVLVGHPWREGDKLYNALSLFAQGKLLGRYFKQQLPNYGVFDEKRYFSAGHQSCVVELKGYRLGLLICEDLWLDGPIDAV KAAGAEVVLSINASPYNREKPYIRKTLMAAHCQRTGLPLVYLNQVGGQDELIFDGCSKVFDAAGNMTHRLAAFAEQTTLL QLNECNVIPMTAPVAELPPLAQVYEALVLAVRDYVTKNGFNGAVLGLSGGIDSALTLAIAVDALGKDKVQALMMPFRYTA DISIADAKEEAEILGVKFDVMSIEPMFDAFMGQLAPMFADTGRDTTEENLQARCRGVVLMALSNKRRSIVLTTGNKSEMA VGYATLYGDMAGGFDVLKDVPKTLVFKLAEYRNTVSYVIPQRVITRPPSAELAPDQKDEDSLPPYDILDAILEGYVEQDK SVAELVADGFDEAIVRKVIRLVDINEYKRRQSAVGPRITARSFGKDRRYPITSGFGRKNW >Mature_539_residues SRSLSIALAQLNWLVGDIEGNTERMLQTLNEQQKAGADLVMFSELALSGYPPEDLLYRDDFYQRCEVQLERLQAATPHIA VLVGHPWREGDKLYNALSLFAQGKLLGRYFKQQLPNYGVFDEKRYFSAGHQSCVVELKGYRLGLLICEDLWLDGPIDAVK AAGAEVVLSINASPYNREKPYIRKTLMAAHCQRTGLPLVYLNQVGGQDELIFDGCSKVFDAAGNMTHRLAAFAEQTTLLQ LNECNVIPMTAPVAELPPLAQVYEALVLAVRDYVTKNGFNGAVLGLSGGIDSALTLAIAVDALGKDKVQALMMPFRYTAD ISIADAKEEAEILGVKFDVMSIEPMFDAFMGQLAPMFADTGRDTTEENLQARCRGVVLMALSNKRRSIVLTTGNKSEMAV GYATLYGDMAGGFDVLKDVPKTLVFKLAEYRNTVSYVIPQRVITRPPSAELAPDQKDEDSLPPYDILDAILEGYVEQDKS VAELVADGFDEAIVRKVIRLVDINEYKRRQSAVGPRITARSFGKDRRYPITSGFGRKNW
Specific function: Can use both glutamine or ammonia as a nitrogen source [H]
COG id: COG0171
COG function: function code H; NAD synthase
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Contains 1 CN hydrolase domain [H]
Homologues:
Organism=Saccharomyces cerevisiae, GI6321865, Length=590, Percent_Identity=23.2203389830508, Blast_Score=74, Evalue=8e-14,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR014445 - InterPro: IPR022310 - InterPro: IPR003694 - InterPro: IPR000132 - InterPro: IPR003010 - InterPro: IPR014729 [H]
Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]
EC number: =6.3.5.1 [H]
Molecular weight: Translated: 59780; Mature: 59649
Theoretical pI: Translated: 5.02; Mature: 5.02
Prosite motif: PS00920 NITRIL_CHT_1 ; PS50263 CN_HYDROLASE
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.3 %Cys (Translated Protein) 2.8 %Met (Translated Protein) 4.1 %Cys+Met (Translated Protein) 1.3 %Cys (Mature Protein) 2.6 %Met (Mature Protein) 3.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSRSLSIALAQLNWLVGDIEGNTERMLQTLNEQQKAGADLVMFSELALSGYPPEDLLYRD CCCCHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCHHCCCHH DFYQRCEVQLERLQAATPHIAVLVGHPWREGDKLYNALSLFAQGKLLGRYFKQQLPNYGV HHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC FDEKRYFSAGHQSCVVELKGYRLGLLICEDLWLDGPIDAVKAAGAEVVLSINASPYNREK CCCHHHHHCCCCEEEEEECCCEEEEEEEEHHCCCCCHHHHHHCCCEEEEEECCCCCCCCC PYIRKTLMAAHCQRTGLPLVYLNQVGGQDELIFDGCSKVFDAAGNMTHRLAAFAEQTTLL HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCEEE QLNECNVIPMTAPVAELPPLAQVYEALVLAVRDYVTKNGFNGAVLGLSGGIDSALTLAIA EECCCCEEECCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHH VDALGKDKVQALMMPFRYTADISIADAKEEAEILGVKFDVMSIEPMFDAFMGQLAPMFAD HHHCCHHHHHHHHHCCEEECCEEEECCCCCHHEEEEEEEEEECCHHHHHHHHHHHHHHHC TGRDTTEENLQARCRGVVLMALSNKRRSIVLTTGNKSEMAVGYATLYGDMAGGFDVLKDV CCCCCCHHHHHHHHCEEEEEEECCCCCEEEEEECCCCCCEEEHHHHHHCCCCCHHHHHHH PKTLVFKLAEYRNTVSYVIPQRVITRPPSAELAPDQKDEDSLPPYDILDAILEGYVEQDK HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCH SVAELVADGFDEAIVRKVIRLVDINEYKRRQSAVGPRITARSFGKDRRYPITSGFGRKNW HHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEHHHCCCCCCCCCCCCCCCCCC >Mature Secondary Structure SRSLSIALAQLNWLVGDIEGNTERMLQTLNEQQKAGADLVMFSELALSGYPPEDLLYRD CCCHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCHHCCCHH DFYQRCEVQLERLQAATPHIAVLVGHPWREGDKLYNALSLFAQGKLLGRYFKQQLPNYGV HHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC FDEKRYFSAGHQSCVVELKGYRLGLLICEDLWLDGPIDAVKAAGAEVVLSINASPYNREK CCCHHHHHCCCCEEEEEECCCEEEEEEEEHHCCCCCHHHHHHCCCEEEEEECCCCCCCCC PYIRKTLMAAHCQRTGLPLVYLNQVGGQDELIFDGCSKVFDAAGNMTHRLAAFAEQTTLL HHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCEEE QLNECNVIPMTAPVAELPPLAQVYEALVLAVRDYVTKNGFNGAVLGLSGGIDSALTLAIA EECCCCEEECCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHH VDALGKDKVQALMMPFRYTADISIADAKEEAEILGVKFDVMSIEPMFDAFMGQLAPMFAD HHHCCHHHHHHHHHCCEEECCEEEECCCCCHHEEEEEEEEEECCHHHHHHHHHHHHHHHC TGRDTTEENLQARCRGVVLMALSNKRRSIVLTTGNKSEMAVGYATLYGDMAGGFDVLKDV CCCCCCHHHHHHHHCEEEEEEECCCCCEEEEEECCCCCCEEEHHHHHHCCCCCHHHHHHH PKTLVFKLAEYRNTVSYVIPQRVITRPPSAELAPDQKDEDSLPPYDILDAILEGYVEQDK HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCH SVAELVADGFDEAIVRKVIRLVDINEYKRRQSAVGPRITARSFGKDRRYPITSGFGRKNW HHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEHHHCCCCCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10910347 [H]